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Topic: Isotopic labeling

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Isotope

Stable isotope

Atomic mass

Infrared spectroscopy

Isotope table (divided)

Radiocarbon dating

Oxygen

Periodic table (standard)

Periodic table

Atomic number

Isotope table (complete)

Nitrogen

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	Isotopic labeling - Biocrawler (Site not responding. Last check: 2007-10-26)
		Isotopic labeling is a technique for tracking the passage of a sample of substance through a system.
		The substance is 'labeled' by including unusual isotopes in its chemical composition.
		A common variation on this procedure is radioisotopic labelling, in which the unusual isotopes are radioactive and detected by their radioactivity.
	www.biocrawler.com /encyclopedia/Isotopic_labeling (220 words)

	Selective isotopic labeling of spin labels for electron spin resonance spectroscopy - Patent 5431901
		The linear increase in signal height with increasing concentration of perdeuterated spin label, at low concentrations of perdeuterated spin label, is eventually overcome by the self-broadening effect of the perdeuterated spin label at higher concentrations of perdeuterated spin label, therefore yielding a linear decrease of the signal height at higher perdeuterated spin label concentrations.
		Therefore, given the sensitivity of the spectral width to perdeuterated spin label concentration, and the difficulty of reliably determining the contribution of perdeuterated spin label concentrations from heterogeneous samples, a reliable and accurate quantitative measurement of oxygen tension from actively metabolizing heterogeneous tissue is difficult.
		Overall, the selectively isotopically-labeled nitroxide spin labels of the present invention increase the sensitivity of the ESR spectrum of a particular environment by maximizing the sensitivity of the T.sub.2 spectrum line-broadening method to oxygen tension and by optimizing the sensitivity of the spectrometer signal for a given concentration of spin label.
	www.freepatentsonline.com /5431901.html (9990 words)

	Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and ...
		Since the enriched isotope is typically heavier than the most abundant naturally occurring isotope (.sup.15 N versus.sup.14 N, for example), the peak at the higher m/z ratio is generally indicative of the relative abundance of the peptide from a labeled protein grown in the medium enriched in one or more isotopes.
		Isotopically labeling one of the two cell pools and observing the ratio between the peaks of the isotopically labeled and non-isotopically labeled peptides also compensates for differential effects between the cell pools themselves, such as the presence of a different number of cells in each, providing an internal normalization between the cell samples.
		As long as the abundance of at least one isotope in one of the media or the food fed one of the animals is different from the abundance of that isotope in the other, the method of the present invention may be applied.
	www.freepatentsonline.com /6391649.html (10506 words)

	Technical Article
		Isotopic labeling has played crucial roles in the development of solid-state NMR spectroscopy as a method for determining the structures of proteins that reside in membranes, aggregates, or other types of assemblies.
		Uniform labeling has the effect of shifting the experimental burdens from the isotopic labeling to the spectroscopy, which is fundamentally more powerful and flexible and now capable of yielding completely resolved spectra of membrane proteins in phospholipids bilayers (Marassi et al., 1997).
		N labeled samples is the basis for an approach to simultaneous resonance assignment and three-dimensional structure determination of membrane proteins in lipid bilayers.
	www.sigmaaldrich.com /Area_of_Interest/Chemistry/Stable_Isotopes__ISOTEC_/Biomolecular_NMR/Alpha_Keto_Acids.html (1891 words)

	Investigation of the Effects of Isotopic Labeling at a PS/PMMA Interface Using SIMS and Mean-Field Theory
		Investigation of the Effects of Isotopic Labeling at a PS/PMMA Interface Using SIMS and Mean-Field Theory
		Isotopic labeling (deuteration) is known to affect the phase behavior of polystyrene (PS) and poly(methyl methacrylate) (PMMA) blends, but little is known regarding the changes in the interfacial properties at the PS/PMMA interface due to deuteration of PS and/or PMMA.
		To investigate these potential changes, secondary ion mass spectrometry (SIMS) was used to measure real-space depth profiles of dPS in hPS:dPS/hPMMA bilayers, with the hPS:dPS blend being well within the single-phase region of the phase diagram.
	pubs.acs.org /cgi-bin/abstract.cgi/mamobx/2006/39/i04/abs/ma052236z.html (333 words)

	CiteULike: A method for efficient isotopic labeling of recombinant proteins (Site not responding. Last check: 2007-10-26)
		A rapid and efficient approach for preparing isotopically labeled recombinant proteins is presented.
		The method is demonstrated for 13C labeling of the C-terminal domain of angiopoietin-2, 15N labeling of ubiquitin and for 2H/13C/15N labeling of the Escherichia coli outer-membrane lipoprotein Lpp-56.
		The expression yields obtained provide a fourfold to eightfold reduction in isotope costs using simple shake flask growths.
	www.citeulike.org /user/viktor21614/article/1624592 (235 words)

	Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR -- Camarero et al. 99 ...
		Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR -- Camarero et al.
		This segmental isotopic labeling pattern was accomplished by
		because the labeled residues are known from the molecular synthesis.
	www.pnas.org /cgi/content/full/99/13/8536 (3032 words)

	[L] TERMS & DEFINITIONS IN NUCLEAR CHEMISTRY
		LABELING, CONJUGATION Labeling of a substance by conjugation with a labeled molecule.
		LABELING, GENERAL Labeling in which compounds are nonuniformly labeled where the position of the radioactive atom in the molecule varies from one molecule to the other.
		LABELING, WILZBACH Labeling of a substance by exposing it to tritium gas.
	www.radiochemistry.org /nomenclature/l.htm (860 words)

	Iostope Usage on SIO Ships
		During recent years there has been great concern about maintaining the utility of our research fleet for the study of natural isotopes and radioisotopes in the marine environment, while at the same time trying also to maintain the usefulness of the fleet for isotopic labeling experiments in which artificial isotopes and radioisotopes are used.
		Isotope users are urged to provide as detailed a protocol as possible for their intended isotope use.
		The isotope user, however, is required to submit a detailed protocol for the isotope usage envisioned at the time a shiptime request is made.
	sio.ucsd.edu /shipsked/general/forms/isotopes (1749 words)

	Reagents and methods for labeling terminal olefins invention
		Uniform labeling yields the labeled molecules in which the isotopes are distributed in a statistically uniform pattern.
		General labeling yields the molecules where the isotopes are distributed in a general or random pattern, not always known with any certainty.
		Nominal labeling is used to indicate the position of the isotopes where there is uncertainty as to whether the labeling is confined to the positions specified.
	www.freshpatents.com /Reagents-and-methods-for-labeling-terminal-olefins-dt20060302ptan20060045846.php?type=description (9431 words)

	Untitled Document
		A variation of pulse labeling is repeated pulse labeling, where labeled CO is applied to plants at different times during the growing season.
		The time period between application of labeled CO and the final experimental measurements is called the "chase period." The selection of the chase period is dependent on the amount of time needed for allocation of the labeled C (Paterson et al., 1997).
		In continuous labeling, plants are exposed to labeled CO throughout the growth of the plant, which results in uniform labeling of all plant C-pools, including labile metabolic substances and more stable structural components.
	www.biology.duke.edu /bio265/ajm21/isotopes.html (1130 words)

	Tutorial : Assay: Protein Production for Structural Analysis Genetic Engineering News - Biotechnology from Bench to ...
		At the end of a 15-minute isotopic labeling at cold shock temperatures, nonradioactive methionine was added and incubated for an additional five minutes.
		Labeled cells were washed in a Tris-Cl, NaCl buffer and then centrifuged to remove the buffer.
		The pulse labeling results demonstrate that using the SPP System, a union of pCold vectors and mazF, up to 90% of the newly synthesized protein expressed is the protein of interest.
	www.genengnews.com /articles/chtitem.aspx?tid=1450 (1377 words)

	In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics
		Stable isotope coding strategies are of immense value in determining protein concentration changes in cells and tissues triggered by regulatory stimuli (e.g., drugs and toxins) and disease (e.g., caused by mutational changes).
		Recognizing and identifying the small number of proteins whose expression levels differ as a consequence of disease or external stimuli are complicated by the complexity of biological extracts and the fact that protein posttranslational modifications are numerous and can occur at many sites on a protein.
		Using the following protocol, stable isotopes can be incorporated into metabolic products, and the relative difference between these products from cells grown in normal or isotope-enriched media can be readily quantified by MS analysis.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Proteomics_and_Protein_Expr/Protein_Expression/Protocols/Labeling_of_Proteins_for_Quantitation.html (361 words)

	DNA Labeling/Non-isotopic Labeling Protocols
		This can be achieved by using the quantification gel for PCR-labeled inserts, in which case start at step 2.
		If other labeling procedures have been used, start with step 1.
		Label fragmented E.coli genomic DNA with CyDye-dUTP directly using Klew Fragment
	www.protocol-online.org /prot/Molecular_Biology/Enzymatic_Manipulation_of_Nucleic_Acid/Labeling/DNA_Labeling/Non-isotopic_Labeling (175 words)

	The Sulfonium Ion Linkage in Myeloperoxidase. DIRECT SPECTROSCOPIC DETECTION BY ISOTOPIC LABELING AND EFFECT OF ...
		DIRECT SPECTROSCOPIC DETECTION BY ISOTOPIC LABELING AND EFFECT OF MUTATION -- Kooter et al.
		There is still debate on the nature of the sulfonium ion linkage, and we tried to specifically investigate this by isotopically
		reduced oxidized spectra of a labeled recombinant MPO are recorded.
	www.jbc.org /cgi/content/full/274/38/26794 (5171 words)

	ISB NHLBI Seattle Proteome Center
		The objective of this course is to instruct active proteomics researchers in the use of a suite of software tools designed for the analysis, validation, storage and interpretation of data obtained from large-scale quantitative proteomics experiments using the ICAT reagent labeling method, multi-dimensional chromatography and tandem mass spectrometry.
		Through lectures and hands-on exercises, each course participant should become proficient in the use of the tools.
		Day 4 - ASAPRatio: Isotopic Labeling and MS-based Quantitative Proteomics - Xiaojun Li
	www.proteomecenter.org /course.php (317 words)

	CARE online \| shop home \| Bruker Daltonics \| Enabling Life Science Tools Based On Mass Spectrometry - Index. Bruker ...
		SERVA-ICPL Kits — NOW without Dry Ice Shipment
		Bruker Daltonics offers and fully supports the ICPL — Isotope Code Protein Labeling technology for quantitative and qualitative analysis of proteins in proteome studies.
		Until now, all SERVA ICPL kits were shipped on dry ice to ensure their proper fu(...)
	www.bdal.de /care (547 words)

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