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| | Title of Invention: Expression of DNA sequences enc oding a thermally stable cytosine deaminase from saccharomyces |
 | | CDase has been used therapeutically for the conversion of the prodrug 5-fluorocytosine (5-FC) to the anticancer drug 5-fluorouracil (5-FU) (Katsuragi et al., 1987; Nishiyama et al., 1985; Sakai et al., 1985; Senter et al., 1987). |
| | The current method of purifying CDase differs from reported methods (see, e.g., Katsuragi et al., 1987; Katsuragi et al., 1986; Ipata et al., 1978; Ipata et al., 1971) as well as from that of Katsuragi, 1988. |
| | Thus, CDase can be coadministered with 5FC such as by surgically placing CDase at or near the tumorous site and administering 5FC orally, as described by Katsuragi et al., 1987, the disclosure of which is incorporated herein by reference. |
| www.nal.usda.gov /bic/Biotech_Patents/1994patents/05338678.html (10209 words) |
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