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Topic: Kinase

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Protein kinase

Pyruvate kinase

Tyrosine kinase

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	C&EN: Latest News - Kinase Assay By Mass Spec
		Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, the Chicago team evaluates kinase activity from the spectra of peptides, immobilized on a gold substrate, before and after exposure to a kinase-mediated phosphorylation.
		The kinase reaction yielded a mass change of 80, due to addition of a phosphate group to each substrate.
		Results for the inhibition of the kinase Ab1 by the anticancer drug Gleevec are within the range of values reported in the literature.
	pubs.acs.org /cen/news/8247/8247notw9.html (278 words)

	TransLucent in vivo Kinase Assay Kit
		Kinases mediate a number of physiological and pathological changes in cell function, including chromatin structure, gene expression, cell proliferation and metabolic activities.
		Inappropriate or deregulated kinase function is often associated with disease states, such as diabetes, cancer, heart disease, neurological and autoimmune diseases.
		A better understanding of the role of specific protein kinases in a particular signaling pathway is key to the understanding of diseases and their ultimate treatment.
	www.panomics.com /TADinvivo.cfm (353 words)

	Kinase
		A kinase is an enzyme designed to transfer phosphate groups from ATP and similar high-energy molecules to specific target molecules called substrates through a process called "phosphorylation." Because of this function, kinases and other enzymes that remove phosphate groups are called phosphatases.
		The most important kinase group are the protein kinases, which act on specific proteins to control complex cellular processes.
		Other kinases act on lipids, carbohydrates, amino acids, nucleotides, and other organic molecules to signal or prime them for metabolic biochemical reactions.
	www.iscid.org /encyclopedia/Kinase (160 words)

	FAQs: Kinase-Glo™ Luminescent Kinase Assay
		Kinase reaction conditions are first optimized with respect to the concentrations of ATP and kinase substrate.
		The optimal amount of kinase to use in subsequent compound screens and IC determinations will be the amount required for luminescence to be within the linear range of the kinase titration curve (sigmoidal dose response).
		The purpose for determining the Z´ factor is to assess the suitability of the assay for high-throughput screening of kinase inhibitors.
	www.promega.com /faq/kinaseglo.html (991 words)

	[No title]
		Creatine kinase, also known as phosphocreatine kinase or creatine phosphokinase, is an enzyme or type of protein that is found in several tissue types of the human body, including the muscle and the brain.
		The function of this enzyme is to catalyze the conversion of creatine to phosphocreatine by applying itself in the consumption of adenosine triphosphate, the generation of adenosine diphosphate, and the reverse reaction.
		A lower than normally low level of creatine kinase shows that you have been drinking excessively; alcohol liver disease and rheumatoid arthritis are two of the most common possibilities that exist with respect to lowered levels of creatine kinase.
	www.creatinejournal.com /creatine-supplement-creatine-kinase (2048 words)

	Kinase.com
		KinBase holds information on over 3,000 protein kinase genes found in the genomes of human, and many other sequenced genomes.
		Yeast and worm data were updated in a comparative Kinase Evolution analysis published in TiBS, and an update on nematode kinases, including those of C.
		We have also recently completed a comprehensive analysis of a wide variety of microbial kinases related to the eukaryotic protein kinase family, using genomic and metagenomic sequences.
	www.kinase.com (218 words)

	Report predicts kinase market to reach $58.6bn in 2010
		Now that many kinases have been sequenced and their structures have been determined, more efficient approaches to small molecule kinase inhibitor discovery are urgently needed.
		Kinases with a particular focus on the development of new assays for use in high throughput screening for potential inhibitors, specificity profiling of candidate drugs, and identifying physiological substrates of targeted kinases.
		The report concluded that although kinase targeted drugs are currently under investigation for over 50 unique indications, most commercial activity centred on the areas of inflammation and tissue injury, diabetes and cardiovascular disease.
	www.drugresearcher.com /news/ng.asp?n=71133-pharmaprojects-kinase-tyrosine (823 words)

	Products: HTRF® KinEASE™
		The kinase is incubated in the presence or absence of compounds and the appropriate substrate.
		For each enzyme the kinase, substrate and ATP concentrations used were KM values determined earlier in assay optimization process.
		Kinase reactions were run using various concentrations of Staurosporine for 30 minutes at room temperature followed by the addition of Eu3+ Cryptate labeled antibody and SA-XL665.
	www.htrf.com /products/kinase_oncology/kinease (589 words)

	Tyrosine Kinase Overview
		Of the 91 protein tyrosine kinases identified thus far, 59 are receptor tyrosine kinases and 32 are non-receptor tyrosine kinases.
		The intracellular kinase domains of receptor PTKs can be divided into two classes: those containing a stretch of amino acids separating the kinase domain and those in which the kinase domain is continuous.
		Activation of the kinase is achieved by ligand binding to the extracellular domain, which induces dimerization of the receptors.
	www.sigmaaldrich.com /Area_of_Interest/Biochemicals/Enzyme_Explorer/Key_Resources/Protein_Kinase_Explorer/Tyrosine_Kinase_Overview.html (714 words)

	[No title]
		Protein kinase C (PKC), a family of related serine-threonine kinases, is a key player in the cellular responses mediated by the second messenger diacylglycerol (DAG) and the phorbol ester tumor promoters.
		The data presented here show that the activity of p38 kinase is significantly inhibited by toxin A, Gd3+ ions, or EGTA treatment whereas no effect of these treatments was observed on the activity of ERK1/2.
		PKC constitutes a family of serine-threonine kinases that catalyze numerous biochemical reactions critical to the function of many cellular constituents [20, 21].
	www.lycos.com /info/protein-kinase--activation.html (908 words)

	Drug Discovery: Focus on Small Molecule Kinase Inhibitors - Health - RedOrbit (Site not responding. Last check: )
		In order to comprehensively assess recent progress and prospects in the kinase field, several surveys were carried out exclusively for this report, involving searches of the Pharmaprojects database and the U.S. Patent and Trademark Office database, and questioning of respondents from the pharmaceutical industry.
		Cancer and other proliferation-related disorders are associated with stimulation of intra-cellular signaling, and since kinases (most of the time) positively relay signaling events; their inhibition offers a powerful way to block aberrant signal transduction cascades.
		The focus throughout this report is on inhibitors of protein kinases, although, where appropriate, protein kinase stimulators are also discussed, as are modulators of lipid and sugar kinases.
	www.redorbit.com /news/health/675180/drug_discovery_focus_on_small_molecule_kinase_inhibitors/index.html?source=r_health (942 words)

	Promega Kinase-Glo Method for Veritas multiwell plate microplate luminometer from Turner BioSystems
		Kinases constitute a large class of enzymes that phosphorylate specific amino acids according to their original recognition motif.
		It is strongly recommended to optimize the kinase reaction with respect to the amount of ATP and kinase substrate.
		The optimal kinase concentration should be an amount that results in luminescence in the linear range of the kinase titration curve.
	www.turnerbiosystems.com /doc/appnotes/S_0088.html (1296 words)

	Pyruvate kinase deficiency
		Pyruvate kinase deficiency is an inherited deficiency of the enzyme pyruvate kinase, which is used by red blood cells.
		Pyruvate kinase deficiency is transmitted as an autosomal recessive trait, which means that a child must get the defective gene from both parents to develop the disorder.
		Although pyruvate kinase deficiency is found in people of all ethnic backgrounds, certain populations, such as the Amish, have a somewhat higher incidence.
	www.pennhealth.com /ency/article/001197.htm (551 words)

	Protein Kinase Inhibitor Specificity
		Communi, et al., Calcium-calmodulin-dependent protein kinase II and protein kinase C-mediated phosphorylation and activation of D-myo-inositol 1,4, 5-trisphosphate 3-kinase B in astrocytes.
		Alessi, et al., PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo.
		Cambien, et al., Src-regulated extracellular signal-related kinase and syk-regulated c-Jun N-terminal kinase pathways act in conjunction to induce IL-1 synthesis in response to microtubule disruption in HL60 cells.
	www.sigmaaldrich.com /Area_of_Interest/Biochemicals/Enzyme_Explorer/Key_Resources/Protein_Kinase_Explorer/PK_Inhibitor_Specificity.html (3242 words)

	Acumen Explorer Applications - protein kinase activation
		In a whole cell environment, all components required for kinase activation are present in native quantities, thereby eliminating stoichiometric imbalances.
		Validated protocols exist for ERK and JNK which are readily adapted to other kinases or transcription factors.
		Most use a phosphospecific antibody to signify protein kinase activation and are detected using a FITC or Alexa 488 secondary conjugate.
	www.ttplabtech.com /acumen/apps_kinase.htm (154 words)

	MedlinePlus Medical Encyclopedia: Pyruvate kinase
		Pyruvate kinase is a blood test to detect abnormally low levels of pyruvate kinase (PK), an enzyme that helps convert glucose to energy when oxygen is low (termed anaerobic metabolism).
		A deficiency in pyruvate kinase is fairly rare, and this test helps your health care provider determine the cause of your anemia.
		The pyruvate kinase level is measured in relation to the amount of hemoglobin (a normal part of red blood cells) in the sample.
	www.nlm.nih.gov /medlineplus/ency/article/003357.htm (598 words)

	Membrane-Bound Dictyostelium Myosin Heavy Chain Kinase: A Developmentally Regulated Substrate-Specific Member of the ...
		A cDNA clone corresponding to the Dictyostelium myosin heavy chain kinase (MHCK) gene was isolated using antibodies specific to the purified enzyme.
		Sequence analysis of the cDNA revealed that the Dictyostelium MHCK possesses all of the domains characteristic of members of the protein kinase C family.
		The amino-terminal region of the MHCK contains the cysteine-rich motif with an internal duplication that is present in all known protein kinase C species.
	www.pnas.org /cgi/content/abstract/89/13/5877 (676 words)

	MAP kinase and its relatives
		MEK and related kinases are in turn phosphorylated and activated by further ser/thr kinases, the MEK kinases or the MAP kinase kinase kinases.
		Often a small G protein is involved, such as ras for the MAP kinases, rac/rho for Jnk etc. The net effect is to funnel a lot of different inputs through the various MAP kinase kinase kinases onto a specific MAP kinase kinase and its associated MAP kinase.
		It may be generally true that kinases associate with scaffolding proteins which funnel their activity to specific substrates, and that these are particularly well characterized in the case of the MAP kinases since these are so well studied.
	www.ufbi.ufl.edu /~shaw/MAPK05.htm (2127 words)

	The Fiercest Competition in Kinase Inhibitor Drug Development is Found in Hematological, Breast, Lung, Prostate and ...
		In this triple analysis report we have analyzed three major and intertwined areas of cancer R&D, Lymphoma, Protein Kinase Inhibitors and Therapeutic Antibodies, which are all subjects to an extensive number of innovative drug candidates.
		The fiercest competition in kinase inhibitor drug development is found in hematological, breast, lung, prostate, and colorectal cancer.
		kinase inhibitors and antibodies in oncology in general.
	www.redorbit.com /news/health/630320/the_fiercest_competition_in_kinase_inhibitor_drug_development_is_found/index.html?source=r_health (594 words)

	B-213 Kinase Inhibitors (Site not responding. Last check: )
		Receptor tyrosine kinase inhibitors comprise 49.8% of the total market in 2005 and are projected to grow at an AAGR of 26.6% to account for 63.7% of the total in 2010.
		The major disease application for kinase inhibitors is oncology that comprised nearly 90% of kinase inhibitor sales in 2005 and is expected to grow at an AAGR of 21.5%.
		The field of kinase inhibitor development is still immature and the reader is cautioned to use the report data as a guide to assess trends, developments and challenges that the industry faces.
	www.bccresearch.com /bio/B213.html (1499 words)

	Kinase Assays: Specificity And Speed - Biocompare Buying Tips
		Just as there are many types of kinases in the “kinome” — the total collection of functional kinases in an organism’s living cells — so are there many kinds of kinase assays that researchers use to study them.
		If you are a novice when it comes to measuring the ability of your kinase of interest to phosphorylate a substrate (or the degree to which your substrate of interest acquires a phosphate group due to the phosphorylation activity of a kinase), the variety of kinase assays from which to choose can be befuddling.
		Kinase assays are continually getting better as their users ask vendors for more options.
	www.biocompare.com /buyingtip/36/Kinase-Assays-Specificity-And-Speed.html (1502 words)

	Promega Kinase-Glo® Plus Luminescent Kinase Assay
		Can be used for a wide range of kinases (including lipid, sugar and alcohol kinases) and substrates (peptides, proteins, lipids, sugars and alcohols).
		The kinase reaction is conducted under the appropriate conditions.
		There is a linear relationship between the luminescent signal and the amount of ATP in the kinase reaction from 0.100µM to 100µM (r2 = 0.9993).
	www.promega.com /kinasegloplus (399 words)

	Chemicon - Product #SGT410 - Tyrosine Kinase Activity Assay
		Protein tyrosine kinase activity is often associated with membrane receptor protein tyrosine kinases (e.g.
		The fraction of phosphorylated substrate is visualized using a phosphotyrosine monoclonal antibody conjugated to HRP and an ensuing chromagenic substrate reaction.
		Kinase inhibitors are required for stopping the kinase reaction after incubation with the peptide substrate.
	www.chemicon.com /browse/productdetail.asp?ProductID=SGT410 (798 words)

	GE Healthcare Life Sciences - Kinase Assays
		The EFC signal generated is proportional to the amount of phosphopeptide generated by the kinase enzyme.
		A kinase reaction is composed of kinase substrate, enzyme, ATP, compound and any co-factor required for activating phosphorylation.
		Because the components of the kinase reaction are specific to each kinase, each component and incubation time for the kinase should be optimized.
	www4.amershambiosciences.com /aptrix/upp01077.nsf/Content/discoverx_site~kinase_assays (161 words)

	Protein Kinase A
		The unique characteristic of protein kinase A is that its activity is regulated by fluctuating levels of cyclic AMP within cells (hence its alias as the cyclic AMP-dependent protein kinase).
		The classical example is that protein kinase A phosphorylates the enzyme phosphorylase kinase, which, in turn, phosphorylates glycogen phorphorylase, which leads to breakdown of glycogen in liver and muscle.
		Another medically important example is that protein kinase A phosphorylates and thus activates a chloride channel important in secretion of water in the small intestine.
	arbl.cvmbs.colostate.edu /hbooks/molecules/pka.html (816 words)

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