
	Where results make sense

Topic: Kinetochores

Related Topics

Spindle checkpoint

Centromere

Saltwater aquarium

Cygnus Arm

Poecilia latipinna

History of nuclear weapons

Impotence

Stellar dynamics

Exosphere

Manhattan Project

USA PATRIOT Act

Poecilia

In the News (Tue 1 Dec 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	MIT Department of Biology: Peter K. Sorger (Site not responding. Last check: 2007-09-16)
		Analysis of budding yeast kinetochores in vitro: Kinetochores are multi-protein complexes that assemble on centromeric DNA and mediate the attachment of chromosomes to microtubules.
		Kinetochores have the remarkably ability to couple the energy of microtubule polymerization and depolymerization into the forces required for chromosome movement.
		Selected kinetochore proteins are also being subjected to high resolution crystallographic analysis in collaboration with Stephen Harrison’s lab at Harvard Medical School.
	web.mit.edu /biology/www/facultyareas/facresearch/sorger.shtml (1065 words)

	Kinetochores (Site not responding. Last check: 2007-09-16)
		In metazoans, the kinetochores are 100-500 nm in diameter and attach to from 3 to 40 microtubules.
		Kinetochores are located in the primary constrictions of chromosomes.
		Attachment of a pair of kinetochores to microtubules can be amphitelic (to opposite poles), syntelic (to the same pole), monotelic (only one kinetochore connected) or mesotelic (one kinetochore connected to both poles (rev).
	opbs.okstate.edu /~melcher/MG/MGW1/MG1365.html (658 words)

	spindle checkpoint (Site not responding. Last check: 2007-09-16)
		To achieve proper segregation, the two kinetochores on the sister chromatids must be attached to opposite spindle poles.
		When sister kinetochores are properly attached to opposite spindle poles, forces in the mitotic spindle generate tension at the kinetochores.
		Kinetochores that are attached to the mitotic spindle but that are not under tension also trigger the spindle checkpoint.
	en.mcfly.org /spindle_checkpoint (125 words)

	Chromosome motors on the move: From motion to spindle checkpoint activity -- Brunet and Vernos 2 (8): 669 -- EMBO ...
		Brunet, S., Maria, A.S., Guillaud, P., Dujardin, D., Kubiak, J.Z. and Maro, B. (1999) Kinetochore fibers are not involved in the formation of the first meiotic spindle in mouse oocytes, but control the exit from the first meiotic M phase.
		King, J.M., Hays, T.S. and Nicklas, R.B. (2000) Dynein is a transient kinetochore component whose binding is regulated by microtubule attachment, not tension.
		Rieder, C.L. and Alexander, S.P. (1990) Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells.
	emboreports.npgjournals.com /cgi/content/full/2/8/669 (3162 words)

	A Simple, Mechanistic Model for Directional Instability during Mitotic Chromosome Movements -- Joglekar and Hunt 83 ...
		The two kinetochores are connected to each other via the center spring to simulate the observed strains across the kinetochores.
		The trailing kinetochore loses kMTs that switch to shortening of the ejection forces are low and if the leading kinetochore has a larger number of such kMTs.
		Kinetochore microtubules shorten by loss of subunits at the kinetochores of prometaphase chromosomes.
	www.biophysj.org /cgi/content/full/83/1/42 (8772 words)

	Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I -- ...
		Sister proximal telomeric sequences, as detected with the anti-Rap1 antibody, are detected as a pair of dots partially colocalizing with the middle region of the T-shaped SCP3 signal (H).
		Sister kinetochores are closely associated during metaphase I. (A) Section of a metaphase I spermatocyte.
		Trilaminar sister kinetochores are tightly associated at the surface of the centromere, and associated with microtubules emanating from the same pole.
	jcs.biologists.org /cgi/content/full/117/7/1221 (7427 words)

	The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in ...
		and the intensity of Bub1 at kinetochores was diminished (Fig.
		The Aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous chromosomes during meiosis.
		Kinetochore localisation and phosphorylation of the mitotic checkpoint components Bub1 and BubR1 are differentially regulated by spindle events in human cells.
	www.jcb.org /cgi/content/full/161/2/281 (7479 words)

	Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with ...
		The chromosome distribution of an uninjected prometaphase cell is shown (a) with an enlargement of a kinetochore from that cell (b).
		Kinetochores of the injected cell (d) show a clear trilaminar structure with microtubules embedded in the outer plate.
		Pairs of kinetochores in the Cdc34- injected and control cell and the corresponding MCAK localization (arrows) are indicated.
	www.jcb.org /cgi/content/full/154/4/707 (5474 words)

	Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells -- ...
		Staining of the spindle for Mad2 and Bub1 is observed during metaphase, and weak staining of centrosomes (arrowheads) is observed for all proteins at anaphase.
		The distance between sister kinetochores in taxol-treated metaphase cells (right panel) is shorter than in control metaphase cells (middle panel) and closer to the distance in prophase cells (left panel).
		Dephosphorylation of kinetochore phosphoepitopes releases BubR1 and Bub3.
	jcs.biologists.org /cgi/content/full/117/9/1757 (7909 words)

	NEW SECRETS OF CELL DIVISION: Familiar Proteins Play an Unsuspected Role
		Spindles that pull the chromosome and its copy apart attach to the centromere region: kinetochores fasten the centromeres to the spindles.
		These spindles attach to constricted regions of chromosomes called centromeres: complexes of proteins called kinetochores fasten the centromeres to the spindles.
		To elucidate this process, Kaufman and his graduate students, Judith Sharp and Alexa Franco, focused their research on Saccharomyces cerevisiae, the familiar single-celled organism used for centuries to ferment beer and wine and to cause bread dough to rise.
	www.lbl.gov /Science-Articles/Archive/LSD-kinetochores.html (743 words)

	Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle ...
		D shows a histogram of flux velocities for kinetochore fibers (red) and for interpolar spindle fibers (green) obtained from the slopes of kymographs such as those in B and C. Note they have a similar distribution, with nonkinetochore flux being slightly faster.
		When polymerization is slow enough, the kinetochore switches to depolymerization, where speckles are seen to move toward and disappear at the kinetochores.
		The kinetochore in A persists in depolymerization, whereas the kinetochore in B switches back to polymerization during the interval analyzed.
	www.jcb.org /cgi/content/full/162/3/377 (3728 words)

	Localization of Mad2 to Kinetochores Depends on Microtubule Attachment, Not Tension -- Waters et al. 141 (5): 1181 -- ...
		in response to tension at the kinetochore (Nicklas, R.B., S.C. Ward, and G.J. Gorbsky.
		Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint.
		Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to unattached kinetochores.
	www.jcb.org /cgi/content/full/141/5/1181 (6547 words)

	Yeast Kinetochores Do Not Stabilize Stu2p-dependent Spindle Microtubule Dynamics -- Pearson et al. 14 (10): 4181 -- ...
		kinetochore, is the foremost governor of microtubule dynamics.
		that the kinetochore is not required for microtubule stabilization.
		The Caenorhabditis elegans Kinetochore Reorganizes at Prometaphase and in Response to Checkpoint Stimuli
	www.molbiolcell.org /cgi/content/abstract/14/10/4181 (406 words)

	POLO-LIKE KINASE-1 RECRUITS SPINDLE CHECKPOINT MOLECULES TO CENTROMERES AND CREATES THE TENSION-SENSING 3F3/2 ...
		The amount of Cdc20, a substrate specific activator of APC/C, was significantly reduced at the kinetochores of Plk1-depleted cells; three hours after entry into M-phase the intensity of Cdc20 fluorescence at the kinetochores had decreased by 80 ± 13% compared to the control cells.
		The 3F3/2 phosphoepitope is expressed at kinetochores that lack mechanical tension and disappears when tension, induced by attachment of spindle microtubules, is applied.
		We hypothesize that Plk1 phosphorylation of the APC/C at kinetochores may be regulated by tension and may contribute to signaling by the mitotic spindle checkpoint.
	www.med.miami.edu /mnbws/Kallio-.html (510 words)

	Yeast Kinetochores Do Not Stabilize Stu2p-dependent Spindle Microtubule Dynamics -- Pearson et al. 14 (10): 4181 -- ...
		in nucleation of new kinetochore microtubules and persistent
		Nicklas, R.B. The motor for poleward chromosome movement in anaphase is in or near the kinetochore.
		Skibbens, R.V., Skeen, V.P., and Salmon, E.D. Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism.
	www.molbiolcell.org /cgi/content/full/14/10/4181 (6014 words)

	Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes -- De Wulf et al. 17 (23): 2902 -- ... (Site not responding. Last check: 2007-09-16)
		Janke, C., Ortiz, J., Lechner, J., Shevchenko, A., Magiera, M.M., Schramm, C., and Schiebel, E. The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control.
		Li, Y., Bachant, J., Alcasabas, A.A., Wang, Y., Qin, J., and Elledge, S.J. The mitotic spindle is required for loading of the DASH complex onto the kinetochore.
		Luykx, P. The structure of the kinetochore in meiosis and mitosis in Urechis eggs.
	www.genesdev.org /cgi/content/full/17/23/2902 (7718 words)

	Protein dynamics at the kinetochore: cell cycle regulation of the metaphase to anaphase transition -- GORBSKY et al. 13 ...
		the kinetochores of unaligned chromosomes and is broadcast to
		The active inhibitor complex (indicated by fl shading) is released from the kinetochore and blocks the ability of the anaphase-promoting complex/cyclosome (APC/C) to ubiquitinylate target anaphase inhibitor proteins such as Pds1 in budding yeast and Cut2 in fission yeast.
		However, as long as unattached kinetochores persist, active inhibitor complex is continuously regenerated, perhaps by recycling inactivated subunits.
	www.fasebj.org /cgi/content/full/13/9002/S231 (2279 words)

	KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans -- Desai et al. 17 (19): ... (Site not responding. Last check: 2007-09-16)
		KNL-1 is downstream of CeCENP-A and CeCENP-C in the kinetochore assembly hierarchy.
		HCP-1, and CeCLASP-2 in the kinetochore assembly hierarchy.
		Cleveland, D.W., Mao, Y., and Sullivan, K.F. Centromeres and kinetochores: From epigenetics to mitotic checkpoint signaling.
	www.genesdev.org /cgi/content/full/17/19/2421 (6607 words)

	Profile
		Chromosomes segregate using their kinetochores, the specialized protein structures that are assembled on centromeric DNA sequences and attach to spindle microtubules.
		Defects in assembling bioriented kinetochore attachments are detected by the spindle checkpoint that halts the cell cycle until the errors are corrected.
		Ipl1 is also required to activate the spindle checkpoint when there is a defect in kinetochore tension but not attachment and we are currently trying to determine how Ipl1 senses tension defects.
	myprofile.cos.com /sbiggins (1053 words)

	Intra-oocyte Localization of MAD2 and Its Relationship with Kinetochores, Microtubules, and Chromosomes in Rat Oocytes ...
		Confocal micrographs of immunostaining of kinetochores, MAD2, microtubules, and DNA (nucleus) of rat oocytes during meiotic maturation.
		Confocal micrographs of costaining of kinetochores and MAD2 of rat oocytes at GV and ProM-I stages.
		Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation.
	www.biolreprod.org /cgi/content/full/71/3/740 (5835 words)

	Kinetochores Prevent Repair of UV Damage in Saccharomyces cerevisiae Centromeres -- Capiaghi et al. 24 (16): 6907 -- ...
		Kinetochores Prevent Repair of UV Damage in Saccharomyces cerevisiae Centromeres -- Capiaghi et al.
		Kinetochores Prevent Repair of UV Damage in Saccharomyces cerevisiae Centromeres
		by disruption of the kinetochore structure in a ndc10-1 mutant,
	mcb.asm.org /cgi/content/abstract/24/16/6907 (230 words)

	Protein dynamics at the kinetochore: cell cycle regulation of the metaphase to anaphase transition -- GORBSKY et al. 13 ...
		Protein dynamics at the kinetochore: cell cycle regulation of the metaphase to anaphase transition -- GORBSKY et al.
		Protein dynamics at the kinetochore: cell cycle regulation of the metaphase to anaphase transition
		at the kinetochore: cell cycle regulation of the metaphase to
	www.fasebj.org /cgi/content/abstract/13/9002/S231 (221 words)

	The Effects of Vinflunine, Vinorelbine, and Vinblastine on Centromere Dynamics -- Okouneva et al. 2 (5): 427 -- ...
		on tension at the kinetochores and/or on occupancy of the kinetochores
		at the kinetochores (22) and because they are connected to the
		Waters, J. C., Skibbens, R. V., and Salmon, E. Oscillating mitotic newt lung cell kinetochores are, on average, under tension and rarely push.J. Cell Sci.
	mct.aacrjournals.org /cgi/content/full/2/5/427 (5247 words)

	Kelly Dawe's Lab :: Research Interests :: Kinetochores
		Kelly Dawe's Lab :: Research Interests :: Kinetochores
		Our long-term goal is to identify the complete collection of inner kinetochore proteins, and to develop a model for how these proteins are organized. We intend to pursue the tried-and-true method of identifying candidate inner kinetochore proteins by homology to animal and yeast counterparts (e.g.
		MIS12 and HEC1), but will move to proteomics as the maize genome sequence improves. Co-immunoprecipitation will be an important strategy for demonstrating interactions with other proteins, DNA, and RNA. We are also working on novel microscopy-based methods to further this work.
	www.botany.uga.edu /~kelly/researchInterests/kinetochores.html (177 words)

Try your search on: Qwika (all wikis)