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Topic: Kluyveromyces fragilis

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Yeast

Ascomycota

	Kluyveromyces
		fragilis, synchronized by various induction and selection procedures, revealed that there is a fairly steady fall in magnesium concentration as cells grow, terminating in a rapid influx of magnesium just before cell division.
		fragilis by flow microcalorimetry; Beezer AE et al.; The use of microcalorimetry in the routine identification of microorganisms is critically discussed and assessed.
		fragilis the role of physical parameters and that of oxygen tension are discussed.
	www.bionewsonline.com /4/1/kluyveromyces_c.htm (0 words)

	Kluyveromyces
		fragilis contain an ADH, the electrophoretical mobility of which is identical to that of the ADH of the groundplasma.
		fragilis and their relation to the cell cycle; Penman CS et al.; Protein fractions were obtained from
		fragilis by pH titration, a technique used for histone extraction, following the inclusion of a distilled water extraction stage, and the fractions partially characterized.
	www.bionewsonline.com /4/2/kluyveromyces_c.htm (0 words)

	International Journal of Chemical Reactor Engineering
		The biosorption of cadmium metal ions by Saccharomyces cerevisiae and Kluyveromyces fragilis yeast cells was studied under various operating conditions in a batch system using shaker flasks and a 2-L double draft tube fluidized bioreactor system.
		The effects of key parameters such as solution pH, initial metal concentration, biosorbent concentration, and age of the biosorbent cells were investigated.
		The maximum uptake capacity was estimated to be close to 35 and 40 mg Cd/g DW cell for S. cerevisiae and K. fragilis, respectively at a pH value of 5.0.
	www.bepress.com /ijcre/vol1/A47 (0 words)

	FDA/CFSAN: Agency Response Letter: GRAS Notice No. GRN 000088
		The subject of the notice is a group of ten microbially derived enzyme preparations, i.e., carbohydrase, pectinase, protease, glucose oxidase and catalase enzyme preparations from Aspergillus niger, carbohydrase and protease enzyme preparations from Aspergillus oryzae, carbohydrase enzyme preparation from Rhizopus oryzae, invertase enzyme preparation from Saccharomyces cerevisiae, and lactase enzyme preparation from Kluyveromyces marxianus.
		The notice informs FDA of the view of the Enzyme Technical Association (ETA) that these enzyme preparations are GRAS, through common use in food, for use as enzymes in catalyzing specific reactions in the processing of food.
		marxianus (formerly known as Saccharomyces fragilis and Kluyveromyces fragilis) are described in standard compendia (Refs.
	www.cfsan.fda.gov /~rdb/opa-g088.html (1589 words)

	NationMaster - Encyclopedia: Kluyveromyces fragilis (Site not responding. Last check: )
		EXAMPLE VI Kluyveromyces fragilis NRRL Y-2264 was grown under aqueous aerobic fermentation conditions employing a fermentation temperature of 37.degree.
		To 50 mL volume samples of the Kluyveromyces fragilis, each sample containing about 4.5 g (9 weight percent) yeast cells, was added sufficient dilute sodium sodium hydroxide to adjust the pH to about 7.5.
		Kluyveromyces fragilis Y-2264 was again grown under aqueous aerobic fermentation conditions similarly as described in Example VI above, employing a pH of about 4.7, fermentation temperature 37.degree.
	www.nationmaster.com /encyclopedia/Kluyveromyces-fragilis (248 words)

	Sepulchritude Forum
		"Kluyveromyces fragilis" is I guess what I was looking for.
		They ferment lactose with a Kluyveromyces fragilis yeast strain.
		Carbery also produces grain alcohol, and apparently it is the latter they use to make Boru Vodka, not the lactose-feedstock product.
	www.feeverte.net /archive/messages/16/972.html (0 words)

	Histone-like protein fractions of Kluyveromyces fragilis and their relation to the cell cycle.
		Histone-like protein fractions of Kluyveromyces fragilis and their relation to the cell cycle.
		Penman CS; Duffus JH Protein fractions were obtained from Kluyveromyces fragilis by pH titration, a technique used for histone extraction, following the inclusion of a distilled water extraction stage, and the fractions partially characterized.
		The inclusion of a distilled water step resulted in a tenfold purification of the fraction obtained at pH 2.20 as compared with pH titration alone.
	www.medscape.com /medline/abstract/9743 (202 words)

	Directory of open access journals
		Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning
		PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-b-D-galactopyranoside (APGP) was attached to the activated PEG 4000.
		A new two-step method for extraction and purification of the enzyme b-galactosidase from Kluyveromyces fragilis was developed.
	www.doaj.org /doaj?func=abstract&id=9158&recNo=6&toc=1 (177 words)

	Revista de Microbiologia - Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity ... (Site not responding. Last check: )
		fragilis was fermented and the cells were disrupted with chloroform.
		According to the literature, the molecular mass of ß-galactosidase from Kluyveromyces fragilis is 201 KDa (20), calculated by size exclusion chromatography.
		As the enzyme from Kluyveromyces fragilis is very hydrophilic, being partitioned with the main contaminant proteins to the saline phase in conventional PEG/phosphate systems, a new two-step process was developed for the extraction and purification of ß-galactosidase.
	www.scielo.br /scielo.php?pid=S0001-37141999000400006&script=sci_arttext (3543 words)

	Kluyveromyces Fragilis - Karr.net (Site not responding. Last check: )
		Kluyveromyces marxianus is a species of yeast in the genus Kluyveromyces, and is the sexual form (teleomorph) of Candida kefyr.
		Find kluyveromyces fragilis info at Netster.com - Netster.com makes it fast and easy to find kluyveromyces fragilis information.
		Looking For kluyveromyces fragilis - Find kluyveromyces fragilis and more at Lycos Search.
	209.197.127.220 /encyclopedia/Kluyveromyces_fragilis (183 words)

	Highly stable recombinant yeasts for the production of recombinant proteins - Patent RE37767
		Yeast of the Kluyveromyces genus exhibiting the Pgk.sup.- phenotype.
		A host/vector pair which is stable in a complex medium, wherein the host is a yeast of the Kluyveromyces genus lacking a functional phosphoglycerate kinase gene, and wherein the vector comprises a functional copy of the phosphoglycerate kinase gene.
		Another subject of the invention relates to the expression vectors for yeasts of the Kluyveromyces genus carrying a functional copy of a gene which is essential for the growth of the Kluyveromyces yeast on a complex medium.
	www.freepatentsonline.com /RE37767.html (7887 words)

	Solvent-induced autolysis of cells - Patent 4795709
		Typical applications include Kluyveromyces fragilis for lactase, Pichia pastoris for alcohol oxidase.
		The aqueous slurry of the yeast cells was adjusted to a pH of 7.5 employing concentrated sodium hydroxide.
		Additional portions of the Kluyveromyces fragilis, shown as described above, were employed.
	www.freepatentsonline.com /4795709.html (3548 words)

	Kluyveromyces lactis killer system: analysis of cytoplasmic promoters of the linear plasmids -- Schickel et al. 24 ...
		All of the 14 genes encoded by the cytoplasmic linear killer plasmids of Kluyveromyces lactis are preceded by upstream conserved sequences (UCSs), cis-acting elements involved in plasmid gene transcription.
		In the dairy yeast Kluyveromyces lactis linear DNA plasmids (pGKL1 and pGKL2) are associated with a killer phenotype (1).
		The plasmids can be transfered to and stably maintained in several different yeasts such as Saccharomyces cerevisiae, Candida pseudotropicalis and Kluyveromyces fragilis (1, 19, 20).
	nar.oxfordjournals.org /cgi/content/full/24/10/1879 (3711 words)

	Bioline International Official Site (site up-dated regularly)
		The use of whey for the production of yeast biomass has the advantages that it is a simple treatment process, and the final discharge of the whey is facilitated since the pollutant load is significantly reduced and the whey lactose is converted into yeast biomass.
		The SCP could be produced from whey using yeasts such as Kluyveromyces, Candida and Trichosporon, as they are naturally able to metabolize lactose (Mansour et al.
		Samples containing whey, yogurt and cheese were collected from dairy producing centers in the City of Isfahan in sterile 200 mL bottles and brought to the laboratory in a cooler box.
	www.bioline.org.br /request?ej04028 (3234 words)

	Species Fungorum - Species synonymy
		Dekkeromyces fragilis (A. Jörg.) Santa María and Sánchez Nieto, Boln Inst.
		Guilliermondella fragilis (A. Jörg.) Boidin, Abadie, J.L. Jacob and Pignal, Bull.
		Kluyveromyces fragilis (A. Jörg.) Van der Walt, Antonie van Leeuwenhoek 31: 347 (1965)
	www.indexfungorum.org /Names/SynSpecies.asp?RecordID=316062 (123 words)

	Microbial Cell Factories \| Full text \| Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus ...
		The β-galactosidase from Kluyveromyces lactis is a protein of outstanding biotechnological interest in the food industry and milk whey reutilization.
		Dickson RC, Dickson LR, Markin JS: Purification and properties of an inducible β-galactosidase isolated from the yeast Kluyveromyces lactis.
		Tello-Solís SR, Jiménez-Guzmán J, Sarabia-Leos C, Gómez-Ruíz L, Cruz-Guerrero AE, Rodríguez-Serrano GM, García-Garibay M: Determination of the secondary structure of Kluyveromyces lactis β-galactosidase by circular dichroism and its structure-activity relationship as a function of the pH.
	www.microbialcellfactories.com /content/5/1/41 (4588 words)

	Purification and Characterization of a Carboxypeptidase Y from Kluyveromyces fragilis JSB95 -- Transfiguracion et al. ...
		Purification and Characterization of a Carboxypeptidase Y from Kluyveromyces fragilis JSB95 -- Transfiguracion et al.
		Articles by Transfiguracion, J. Articles by Voort, D. Purification and Characterization of a Carboxypeptidase Y from Kluyveromyces fragilis JSB95
		A carboxypeptidase Y was purified to homogeneity from the crude
	jds.fass.org /cgi/content/abstract/81/3/647 (213 words)

	I
		The remaining yeast in the batch tank (62% protein) was air dried and bagged.
		Kluyveromyces fragilis that has a higher yield of whey to alcohol and requires a two-stage continuous batch fermentor to be efficient.
		Saccharomyces cerevisiae) especially hybridized to convert whey or whey permeate into a high protein food supplement for man or beast.
	wriinc.com /yeast.htm (1218 words)

	Two tryptophans at the active site of UDP-glucose 4-epimerase from Kluyveromyces fragilis -- Ray et al. 270 (19): 11383 ...
		Two tryptophans at the active site of UDP-glucose 4-epimerase from Kluyveromyces fragilis -- Ray et al.
		Two tryptophans at the active site of UDP-glucose 4-epimerase from Kluyveromyces fragilis
		Kluyveromyces fragilis had been reported earlier (Mukherji, S., and
	www.jbc.org /cgi/content/abstract/270/19/11383 (262 words)

	BIP - Improvement of SCP production and BOD removal of whey with mixed yeast culture
		The SCP could be produced from whey using yeasts such as Kluyveromyces, Candida and Trichosporon, as they are naturally able to metabolize lactose (Galvez et al.
		From these, six strains were identified as Kluyveromyces lactis, four strains as Kluyveromyces marxianus and one as Candida versatilis.Comparing to other studies it seems that K.
		This enzyme is produced by many microorganisms that use lactose as energy source (In and Jin, 1998; Uhlig, 1998).
	www.ejbiotechnology.info /content/vol7/issue3/full/7/bip (1670 words)

	Raymond R. Mahoney publications (Site not responding. Last check: )
		Purification and properties of Beta-galactosidase from Kluyveromyces fragilis.
		Stability and enzymatic properties of Beta-galactosidase from Kluyveromyces fragilis.
		Purification and physicochemical properties of Beta-galactosidase from Kluyveromyces fragilis.
	www-unix.oit.umass.edu /~rmahoney/RRMpubs.html (1676 words)

	Enzyme and Microbial Technology. (Site not responding. Last check: )
		Raffael Schaffrath, Kestutis Sasnauskas, Peter A. Meacock, Use of gene shuffles to study the cytoplasmic transcription system operating on Kluyveromyces lactis linear DNA plasmids, Enzyme and Microbial Technology 26 (9-10) (2000) pp.
		Danguol Bartkeviit, Rimantas iektel, Kstutis Sasnauskas, Heterologous expression of the Kluyveromyces marxianus endopolygalacturonase gene (EPG1) using versatile autonomously replicating vector for a wide range of host, Enzyme and Microbial Technology 26 (9-10) (2000) pp.
		-galactosidase activity produced by a thermotolerant strain of Kluyveromyces marxianus during growth on lactose-containing media, Enzyme and Microbial Technology 17 (8) (1995) pp.
	www1.elsevier.com /cdweb/journals/01410229/viewer.htt?viewtype=keywords&rangeselected=136 (728 words)

	Home Distillation of Alcohol (Homemade Alcohol to Drink)
		To hydrolyse lactose you need the enzyme lactase which allows the feedstock to then be fermented by the common Saccharomyces cerevisiae yeasts.
		A principal source of lactase is the yeast Kluyveromyces fragilis which is more commonly used to ferment lactose directly to ethanol.
		Polysaccharides are large complex molecules containing 3 or more monosaccharides (and in some cases number more than several thousand simple sugar molecules) which are used by living organisms to store energy.
	homedistiller.org /sugar.htm (2040 words)

	Isolation, Purification, and Characterization of a Killer Protein from Schwanniomyces occidentalis -- Chen et al. 66 ...
		Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character.
		Efficient isolation of the linear DNA killer plasmid of Kluyveromyces lactis: evidence for location and expression in the cytoplasm and characterization of their terminally bound proteins.
		Ciani, M., Fatichenti, F. Killer Toxin of Kluyveromyces phaffii DBVPG 6076 as a Biopreservative Agent To Control Apiculate Wine Yeasts.
	aem.asm.org /cgi/content/full/66/12/5348 (3352 words)

	Yeast in Animal Feeds
		This yeast is important because it can utilize the pentose sugars from processed wood pulp used in making paper.
		A third useful yeast is Kluyveromyces marxianus, previously classified as Kluyveromyces fragilis.
		This is the Whey Yeast which can utilize milk sugar or lactose as a substrate.
	members.aol.com /ChazStone/Yeast.html (2248 words)

	Index Fungorum - Names Record
		Kluyveromyces fragilis (A. Jörg.) Van der Walt, Bothalia 10(3): 417 (1971)
		Kluyveromyces marxianus (E.C. Hansen) Van der Walt 1971
		Please contact Paul Kirk if you have any additions or errors to report.
	www.indexfungorum.org /Names/NamesRecord.asp?RecordID=316057 (32 words)

	IngentaConnect Degradation of Differently Substituted Xylogalacturonans by Endox... (Site not responding. Last check: )
		Galactosyl- and xylosyl-linkages appeared to be more stable, while some backbone degradation of the galacturonan took place upon prolonged acid treatment.
		Using XGA-35, endoxylogalacturonan hydrolase (XGH) from Aspergillus tubingensis, expressed in Kluyveromyces lactis, was characterised with respect to kinetic parameters, temperature and pH effects.
		tubingensis (PG-arf), from Kluyveromyces fragilis (PG-kluyv) and XGH from A.
	www.ingentaconnect.com /content/tandf/gbab/2003/00000021/F0020004/art00007 (377 words)

	UniProtKB/Swiss-Prot entry P07337 [BGLS_KLUMA] Beta-glucosidase
		They link to the user manual or other documents.
		Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
		Raynal A. Gerbaud C. Francingues M.C. Guerineau M. "Sequence and transcription of the beta-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyces cerevisiae.";
	www.expasy.org /uniprot/BGLS_KLUMA (219 words)

	J. Inst. Brew. vol.109:4 (2003) A Note - Production of Vinegar from Whey (Site not responding. Last check: )
		Cheese whey supplemented with lactose was employed to produce vinegar.
		Whey was first transformed into an alcoholic beverage via fermentation with the yeast Kluyveromyces fragilis, and then the alcoholic product was employed as a substrate for an acetic acid fermentation.
		The bacteria used for the acetic acid production process were isolated from a starter culture provided by a vinegar factory.
	www.scientificsocieties.org /jib/abstracts/2003/G-2003-1215-175.htm (174 words)

	TACTS-META \| Transatlantic Student Mobility
		While in the last decades, the effect of the high pressure in the bioprocess has been white of many studies, is few and dispersed the works on the influence of moderate pressures in the bioprocess.
		In the sequence of previous works on the effect of moderate pressures on the microbial activity, it was intended to study in this work the effect of moderate pressures (up to 6 bar) in the activity and in the thermo-stability of ß-galactosidase of Kluyveromyces fragilis, using the commercial commodity Lactozyme®
		In the present work, a stainless steel reactor was used to carry out discontinuous assays where the reaction mixtures were submitted to air pressures up to 6 bar, comparing the kinetics under pressure with the kinetic at atmospheric pressure.
	www.deb.uminho.pt /tacts-meta/um_abstract01.htm (349 words)

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