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Topic: Knockout mouse

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	Knockout mouse - Wikipedia, the free encyclopedia
		A knockout mouse is a genetically engineered mouse one or more of whose genes have been made inoperable.
		Knockout is a route to learning about a gene that has been sequenced but has an unknown or incompletely known function.
		The Texas Institute for Genomic Medicine is a non-profit institute that generates knockout mice for Academic use.
	en.wikipedia.org /wiki/Knockout_mouse (786 words)

	Gene knockout - Wikipedia, the free encyclopedia
		Also known as knockout organisms or simply knockouts, their most direct use is for learning about a gene that has been sequenced, but has an unknown or incompletely known function.
		Knockout is accomplished through a combination of techniques, beginning in the test tube with a plasmid, a bacterial artificial chromosome or other DNA construct, and proceeding to cell culture.
		Individual cells are genetically transformed with the construct and--for knockouts in multi-cellular organisms--ultimately fused with a stem cell from a nascent embryo.
	en.wikipedia.org /wiki/Gene_knockout (382 words)

	genome.gov \| Knockout Mice
		A knockout mouse is a laboratory mouse in which researchers have inactivated, or "knocked out," an existing gene by replacing it or disrupting it with an artificial piece of DNA.
		For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumors by arresting cell division.
		For example, "Methuselah" is a knockout mouse model noted for longevity, while "Frantic" is a model useful for studying anxiety disorders.
	www.genome.gov /12514551 (1160 words)

	CMC Activity Center : Transgenic and Knockout Mice : Approaches : CMC–Nature Gateway
		This piece of DNA includes the structural gene of interest, a strong mouse gene promoter and enhancer to allow the gene to be expressed and vector DNA to enable the transgene to be inserted into the mouse genome.
		To generate a standard transgenic mouse, a bacterial or viral vector containing the transgene and any desired markers are injected into a fertilized mouse egg.
		The phenotypes of knockout mice can be very complex because all tissues of the mouse are affected, though it is not uncommon for a knockout mouse to display embryonic lethality or to show no phenotype at all.
	www.cellmigration.org /resource/komouse/komouse_approaches.shtml (3019 words)

	Homolgous Recombination & Knockout Mouse
		Mouse is good mammalian system but homologous recombination does not work as efficeintly in human cells.
		A knockout mouse has had both alleles of a particular gene replaced with an inactive allele.
		The pure breeding mouse strain is a "knockout mouse".
	www.bio.davidson.edu /courses/genomics/method/homolrecomb.html (1036 words)

	The Science Creative Quarterly » STUDYING GENE FUNCTION: CREATING KNOCKOUT MICE
		Therefore, knockout animals are considered an investigative technique that allows for a particular gene of interest to be removed in an attempt to define what effect that gene has in the life of the organism.
		The purpose of conditional knockouts, in contrast, is to delete a gene in a particular organ, cell type, or stage of development [3].
		The development of a gene knockout mouse has been a massive advance to the biomedical and pharmaceutical field presenting researchers with a very powerful tool for analyzing gene function during development, as well as in disease.
	www.scq.ubc.ca /?p=264 (2045 words)

	ScienceDaily: Spontaneous Mutation Produces New MAO A/B Knockout Mouse
		The MAO A/B knockout mouse should provide an excellent model in which to address the specific roles of these neurotransmitters and their receptors in anxiety and stress-related disorders.
		The scientists hypothesize that the mutation was due to elevated levels of the MAO B substrate phenylethylamine in the MAO B knockout mice.
		Mouse -- The mouse is a mammal that belongs to one of numerous species of small rodents in the genus Mus and various related genera of the family MuridÃ¦ (Old World...
	www.sciencedaily.com /releases/2004/09/040910075119.htm (1727 words)

	Gene Targeting and Knockout Mouse Facility (Site not responding. Last check: 2007-10-21)
		With these capabilities, the Facility can provide a majority of the "cutting-edge" technologies available in the field of knockout mouse technology, the most notable of which are tissue-specific inducible genetic alterations.
		The Facility has demonstrated expertise in the cryopreservation of mouse embryos and in the utilization of cryopreserved embryos for the generation of chimeric mice.
		Such a blastocyst injection station will significantly increase the efficiency and throughput of knockout mouse production, especially because 1) most embryonic stem cell lines are more amenable to blastocyst injection than to morula aggregation, and 2) blastocyst injection requires fewer manipulation and shorter incubation times outside of the female reproductive tract than does morula aggregation.
	bfs.itlab.musc.edu /urrf/cgi-bin/display/gene (1786 words)

	Whitehead Institute - Knockout Mouse Model
		Piotr Sicinski and his colleagues at the Whitehead Institute developed the mouse strain by replacing the normal mouse cyclin D1 gene with a nonfunctional version in embryonic stem cells.
		Because cyclin D1 is thought to be a vital component of the growth machinery in all cells, the researchers had expected the mutation to be lethal to any embryos developing from these stem cells.
		Their studies showed that the receptors are present at normal levels, leading the research team to conclude that the rapid growth of the mammary epithelium during pregnancy may be cyclin D1-driven.
	www.wi.mit.edu /news/archives/1995/rw_0825.html (930 words)

	Boilerplate Overview: OSRR - Gene Targeting and Knockout Mouse Facility (Site not responding. Last check: 2007-10-21)
		The Gene Targeting and Knockout Mouse Facility has demonstrated expertise in the generation of knockout mice, cre-conditional knockout mice, and sequence-specific mutant mice.
		The facility has also demonstrated the ability to generate non-chimeric, entirely ES cell-derived embryos, and cre-recombinant knockout mice via infection of targeted ES cells with adenovirus that express cre recombinase.
		The Gene Targeting and Knockout Mouse Facility is located within the new integrated laboratory animal facilities on the 7th Floor of the Children’s Research Institute.
	research.musc.edu /bp/osrr_gtkmf.html (359 words)

	ScienceDaily: Mouse Gene Knockout Illuminates How Light Resets Clock
		Using mouse embryonic stem cells, Provencio, Kay and colleagues altered the gene to create a strain of mice that lacked a functioning gene for melanopsin.
		Then, the researchers exposed the melanopsin knockout mice to 15 minutes of blue light at a time in their cycle when normal mice show strong phase delays -- alterations in the time of onset of activity in response to light.
		To confirm this deficit in light input, the knockout mice were exposed to constant white light, which normally would trigger phase adjustments resulting in a longer internal clock day than in constant darkness.
	www.sciencedaily.com /releases/2002/12/021216071528.htm (2089 words)

	RFA-HG-05-007: Completion of a Comprehensive Mouse Knockout Resource
		The ultimate aim of the Knockout Mouse Project is to generate a null-mutant mouse resource comprising a null mutation marked with a reporter of high utility for each gene in mouse strain C57BL/6.
		The importance of the mouse as a model organism was indicated by the inclusion of a goal for the construction of genetic and physical maps of the mouse genome in the initial plan for the Human Genome Project (HGP).
		For example, to date, nearly 60% of the genes in the mouse genome have been knocked out using either gene trapping methods (http://www.igtc.ca) or gene targeting by homologous recombination, both of which, for technical reasons, have usually used strain 129 as the background.
	grants.nih.gov /grants/guide/rfa-files/RFA-HG-05-007.html (13445 words)

	NIH launches effort to place more knockout mice in public repositories
		In the two decades since recombinant DNA technology was first used to produce lines of mice in which specific genes have been disrupted, or "knocked out," such mice have proven to be one of the most powerful tools available to study the function of genes and to create animal models of human disease.
		Researchers have generated knockout mice to serve as useful models of human diseases such as cancer, heart disease, neurological disorders and even obesity.
		To increase the availability of these mouse models, the NIH Knockout Mouse Project has initiated an effort to encourage more NIH-supported researchers to place their knockout mouse lines into public repositories.
	www.eurekalert.org /pub_releases/2006-06/nhgr-nle061206.php (1042 words)

	News & Press Releases - New mdrla Knockout Mouse Now Available From Taconic (Site not responding. Last check: 2007-10-21)
		Germantown, NY - Taconic's mdrla Knockout Mouse is widely used by investigators studying the central nervous system and drug transport.
		Taconic's blood-brain barrier deficient animal model, the mdrla Knockout Mouse, lacks the protective function of the P-glycoprotein as it contains a disruption of the endogenous multi-drug resistant (mdrla) gene.
		The mdrla Knockout Mouse can be used to investigate central neurotoxicity of compounds as well as to test new drug design strategies for promoting or minimizing access to the brain.
	www.taconic.com /news-press/newmdrla.htm (172 words)

	Noc2 knockout mouse patent invention
		Disclosed are a mouse homozygous or heterozygous for the defect of the Noc2 gene, and a tissue and a cell of the mouse.
		The Noc2 knockout mice, which exhibit stress-related insulin hyposecretion and accumulation of secretory granules of increased size and irregular shape in exocrine cells, provide a test system used in the development of therapeutic drugs for related disorders.
		[0001] The present invention relates to a Noc2 knockout mouse, more specifically to a mouse in which the Noc2 gene is disrupted and which exhibits stress-related hyposecretion of insulin.
	freshpatents.com /Noc2-knockout-mouse-dt20060126ptan20060021073.php?... (1036 words)

	Crry, but not CD59 and DAF, is indispensable for murine erythrocyte protection in vivo from spontaneous complement ...
		To ascertain that the increased sensitivity of CD59a knockout mouse erythrocytes to complement lysis was not due to alteration
		in the testis was unaffected in the CD59a knockout mouse, and
		Mouse Crry/p65: characterization of monoclonal antibodies and the tissue distribution of a functional homologue of human MCP and DAF.
	www.bloodjournal.org /cgi/content/full/99/10/3707 (7016 words)

	genome.gov \| The Knockout Mouse Project
		The Knockout Mouse Project is a trans-National Insitutes of Health (NIH) initiative that aims to generate a comprehensive and public resource comprised of mice containing a null mutation in every gene in the mouse genome.
		By capitalizing on efficiencies of scale and a centralized production effort, the project intends to make this catalog of mutants available in mouse strain C57BL/6 for two reasons: it is the most widely used strain, and it is the strain for which complete genome sequence has been made available.
		For each mouse line, the contractors have provided not only the mouse line itself, but also detailed, objective data on the impact of the specific gene deletion on the mouse's phenotype.
	www.genome.gov /17515708 (752 words)

	Spontaneous mutation produces new MAO A/B knockout mouse
		Bethesda, MD - A combination of luck and scientific curiosity has produced a mouse lacking two isoenzymes, MAO A and MAO B, that have been linked to violent criminal behavior and Parkinson's disease.
		By making knockout mice lacking either MAO A or MAO B, Jean C. Shih, a Professor at the University of Southern California School of Pharmacy, and her collaborators previously showed how each isoenzyme functions in the body.
		Looking further into the matter, Dr. Shih determined that a spontaneous mutation in a single nucleic acid base pair in the MAO A gene created a premature stop which prevented it from being made into a protein.
	www.eurekalert.org /pub_releases/2004-09/asfb-smp090704.php (591 words)

	DATABASE OF GENE KNOCKOUTS
		In this section, data regarding the phenotypes rendered by the knockout of various molecules are presented.
		Please send your name, the name of your organization, your address, telephone and fax numbers along with, a brief description of the phenotype of the knockout mouse and the appropriate reference(s) to the editorial office for publication.
		This gene knockout database is created at Frontiers in Bioscience and is authored by Siamak Tabibzadeh and John Lee Elzie.
	www.bioscience.org /knockout/knochome.htm (259 words)

	In vivo Gene Knockout: secured your Knockout mouse creation!
		The constitutive gene knockout approach enables a total inactivation of the gene in any cell.
		In a conditional knockout mouse, the inactivation is restricted to specific tissues.
		Gene knockout - Knock in mice - Transgenics - Transgenesis - Gene knockdown
	www.genoway.com /transgenic-company/knockout-mouse.html (165 words)

	News & Press Releases - New PFP/RAG-2 Double Knockout Mouse (Site not responding. Last check: 2007-10-21)
		Deficient in T, B and NK cells, pfp/rag-2 Double Knockout Mouse models are useful research tools in studying overall regulation of the immune system or specific areas such as: NK or CTL activity, immune suppression and transplantation.
		The pfp/rag-2 Double Knockout Mouse is homozygous for both the disrupted rag-2 gene and the pfp gene.
		The pfp/rag-2 Double Knockout Mouse is currently being evaluated under an STTR (Small Business Technology Transfer) Grant to Taconic from the National Cancer Institute.
	www.taconic.com /news-press/newpfp.htm (266 words)

	Knockout Transgenic Mouse
		what is essentially a Uch-L1 drug to raise its levels in the brain, we were able to restore a great deal of brain activity in a transgenic mouse model of...
		When a mouse that does not have AD is placed in the same cage again, it...
		A Knockout Mouse is a mouse that has had the function of one or more of its genes (usually proteins) deleted or made non-functional.
	www.knockoutmousestation.com (162 words)

	RFA-HG-05-008: A Data Coordination Center for the Knockout Mouse Project (KOMP)
		The NIH plans to support the Knockout Mouse Project (KOMP) through a research network for production of a comprehensive resource of mouse mutants in which every gene in the mouse genome has been knocked out by a null mutation marked with a reporter system of high utility.
		The purpose of this RFA is to solicit applications to develop and implement a Data Coordination Center (DCC) to serve as a centralized resource to track the progress of the project and to deliver this information to the research network, NIH staff, and the public.
		The DCC should provide multiple mechanisms for delivering data about mouse knockout genes, including a simple web interface for standard queries and a genome browser view of the status of mouse genes targeted and generated by the KOMP and other projects.
	grants.nih.gov /grants/guide/rfa-files/RFA-HG-05-008.html (7467 words)

	CCMB produces first Gene Knockout Mouse in India:Director
		He said during the last decade and half researchers have perfected a technology called 'Gene Knockout Technology' that allows them to precisely remove a given gene in a mouse.
		Dr Singh said Gene Knockout Technology has tremendous applications not only in the field of basic biology but also for creation of human disease models and in the drug discovery process.
		He said recognizing the tremendous potential of the technology, CCMB has created a National facility for Transgenic and Gene Knockout Mice with support from the Department of Science and Technology (DST), New Delhi, and the Council of Scientific Industrial Research (CSIR).
	news.webindia123.com /news/Articles/India/20060509/328644.html (222 words)

	NIH Deltagen/Lexicon Knockout Mouse Resource - Center for Rodent Genetics - NIEHS
		The mouse lines will be divided among the NIH-sponsored repositories of the Mouse Regional Resource Center and The Jackson Laboratory and will be distributed to researchers from there.
		The NIEHS contributed $400K to this project and was guaranteed that 10 mouse lines of choice would be included in the 251 lines.
		More information on the knockout mice and data can be found on the Trans-NIH Mouse Initiatives website.
	www.niehs.nih.gov /crg/deltalex.htm (227 words)

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