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A Novel Fluorescent Protein-Based Biosensor for Gram-Negative Bacteria -- Goh et al. 68 (12): 6343 -- Applied and ... lipid A and 7.7 to 29.6% with 15 to 240 ng of lipopolysaccharide. ng of lipid A or 7.5 to 60 ng of lipopolysaccharide). 20 ng of lipid A or 60 ng of lipopolysaccharide, the fluorescence aem.asm.org /cgi/content/full/68/12/6343   (3850 words)

Lipopolysaccharide Prevents Doxorubicin-Induced Apoptosis in RAW 264.7 Macrophage Cells by Inhibiting p53 Activation -- ... Effect of lipopolysaccharide on doxorubicin-induced cell death in peritoneal cells from C3H/HeJ and BALB/c mice. The cells were pretreated with lipopolysaccharide (100 ng/mL) for 30 minutes and cultured with doxorubicin (5 µmol/L) for 24 hours. RAW 264.7 cells were pretreated with lipopolysaccharide (100 ng/mL) and treated with doxorubicin (5 µmol/L) for 1, 3, and 6 hours. mcr.aacrjournals.org /cgi/content/full/3/7/373   (2878 words)

PORPHYROMONAS GINGIVALIS LIPOPOLYSACCHARIDE SIGNALING IN GINGIVAL FIBROBLASTS-CD14 AND TOLL-LIKE RECEPTORS -- Wang and ... Lipopolysaccharide Receptors on Gingival Fibroblasts">Porphyromonas gingivalis Lipopolysaccharide Receptors on Gingival Fibroblasts Lipopolysaccharides of Bacteroides intermedius (Prevotella intermedia) and Bacteroides (Porphyromonas) gingivalis induce interleukin-8 gene expression in human gingival fibroblast cultures. Bacterial lipopolysaccharide induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in macrophages. crobm.iadrjournals.org /cgi/content/full/13/2/132   (6975 words)

Specificity of Rabbit Antisera against Lipopolysaccharide of Acinetobacter -- Pantophlet et al. 36 (5): 1245 -- Journal ... Lipopolysaccharide (LPS) is a common constituent of the outer membrane of gram-negative bacteria (28, 40, 41) and has Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter haemolyticus strain ATCC 17906. Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter junii strain 65. jcm.asm.org /cgi/content/full/36/5/1245   (3334 words)

News - Lipopolysaccharide Binding Protein Associated With Immune And Haemodynamic Derangement In Cirrhosis Levels of lipopolysaccharide binding protein are elevated in ascitic cirrhotic patients with marked immune and haemodynamic derangement. Overgrowth and translocation of intestinal bacteria are common in cirrhosis with ascites, the investigators explain, and may result in activation of monocytes and lymphocytes and also in increased levels of pro-inflammatory cytokines and in enhanced synthesis of nitric oxide. Plasma levels of lipopolysaccharide binding protein, endotoxin, soluble CD14, cytokines, renin, nitrites and systemic vascular resistance were assessed before and four weeks after treatment with either norfloxacin or placebo. www.docguide.com /news/content.nsf/news/8525697700573E1885256C9B00024CA8?OpenDocument&c=&count=10&id=48DDE4A73E09A969852568880078C249   (450 words)

Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced ... Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced tumour necrosis factor {alpha} -- Ruemmele et al. Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced tumour necrosis factor Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD14. gut.bmjjournals.com /cgi/content/full/51/6/842   (4161 words)

Lipopolysaccharide binding protein in preterm infants -- Behrendt et al. 89 (6): F551 -- Archives of Disease in ... Lipopolysaccharide binding protein in preterm infants -- Behrendt et al. Lipopolysaccharide is captured by LBP within the circulation Figure 3  Maximum lipopolysaccharide binding protein (LBP) concentration in infants with neonatal bacterial infection born before or after the onset of labour. fn.bmjjournals.com /cgi/content/full/89/6/F551   (2105 words)

Pertussis toxin inhibition of B cell and macrophage responses to bacterial lipopolysaccharide -- Jakway and DeFranco ... Lipopolysaccharide, a component of the outer membrane of Gram-negative bacteria, activates B lymphocytes and macrophages. These results, combined with the demonstration that lipopolysaccharide inhibits adenylate cyclase activity in P388D1 cells, strongly argues that lipopolysaccharide activation of cells is mediated by a Gi-like receptor-effector coupling protein. Lipopolysaccharide Inhibits Virus-mediated Induction of Interferon Genes by Disruption of Nuclear Transport of Interferon Regulatory Factors 3 and 7. www.sciencemag.org /cgi/content/abstract/234/4777/743   (439 words)

Bacterial Lipopolysaccharide Inhibits Dengue Virus Infection of Primary Human Monocytes/Macrophages by Blockade of ... Lipopolysaccharide (LPS) situated in the outer part of the gram-negative bacterial cell wall is the key effector molecule Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression. Lipopolysaccharide (LPS) activation of human endothelial and epithelial cells is mediated by LPS binding protein and soluble CD14. jvi.asm.org /cgi/content/full/73/4/2650   (5302 words)

Genetic Locus Required for Antigenic Maturation of Rhizobium etli CE3 Lipopolysaccharide -- Duelli et al. 183 (20): ... Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development. Structural characterization of the O-antigenic polysaccharide of the lipopolysaccharide from Rhizobium etli strain CE3. The structures of the lipopolysaccharides from Rhizobium etli strains CE358 and CE359. jb.asm.org /cgi/content/full/183/20/6054   (6515 words)

Use of Monoclonal Antibodies to Lipopolysaccharide for Antigenic Analysis of Coxiella burnetii -- Hotta et al. 41 (4): ... Chemical and immunological characterization of lipopolysaccharides from phase I and phase II Coxiella burnetii. A comparative study of lipopolysaccharides from two Coxiella burnetii strains considered to be associated with acute and chronic Q fever. Antigenic characteristic of the lipopolysaccharides of Coxiella burnetii isolates. jcm.asm.org /cgi/content/full/41/4/1747   (1430 words)

Lipopolysaccharide Heterogeneity: Innate Host Responses to Bacterial Modification of Lipid A Structure -- Dixon and ... Biosynthesis of lipid A. In: Bacterial endotoxic lipopolysaccharides. Biological activities of lipopolysaccharides are determined by the shape of their lipid A portion. Lipopolysaccharide induces the rapid tyrosine phosphorylation of the mitogen-activated protein kinases erk-1 and p38 in cultured human vascular endothelial cells requiring the presence of soluble CD14. jdr.iadrjournals.org /cgi/content/full/84/7/584   (7319 words)

Lipopolysaccharide-Binding Protein Critically Regulates Lipopolysaccharide-Induced IFN-{beta} Signaling Pathway in ... Lipopolysaccharide stimulates p38-dependent induction of antiviral genes in neutrophils independently of paracrine factors. Lipopolysaccharide (LPS)-binding protein in human serum determines the tumor necrosis factor response of monocytes to LPS. Lipopolysaccharide binding protein as a marker of inflammation in synovial fluid of patients with arthritis: correlation with interleukin 6 and C-reactive protein. www.jimmunol.org /cgi/content/full/172/10/6185   (5597 words)

Cytoskeletal Alterations in Lipopolysaccharide-Induced Bovine Vascular Endothelial Cell Injury and Its Prevention by ... Lipopolysaccharide induces apoptosis in a bovine endothelial cell line via a soluble CD14 dependent pathway. Effects of lipopolysaccharide, lipid A, lipid X and phorbol ester on cultured bovine endothelial cells. Bacterial lipopolysaccharide induces actin reorganization, intracellular gap formation and endothelial barrier dysfunction in pulmonary vascular endothelial cells: concurrent F-actin depolymerization and new actin synthesis. cdli.asm.org /cgi/content/full/7/2/218   (3769 words)

lipopolysaccharide, Primary Sclerosing Cholangitis Literature Dikopoulos N, Weidenbach H, Adler G, Schmid RM 2003 Lipopolysaccharide represses cholesterol 7-alpha hydroxylase and induces binding activity to the bile acid response element II. Hebeis BJ, Vigorito E, Turner M 2004 The p110delta subunit of phosphoinositide 3-kinase is required for the lipopolysaccharide response of mouse B cells. Hojo M, Sano N, Takikawa H 2003 Effects of lipopolysaccharide on the biliary excretion of bile acids and organic anions in rats. www.psc-literature.org /lps.htm   (11277 words)

High concentrations of lipopolysaccharide-binding protein in serum of patients with severe sepsis or septic shock ...   (Site not responding. Last check: 2007-10-08) Lipopolysaccharide binding protein and serum amyloid A secretion by human intestinal epithelial cells during the acute phase response. Lipopolysaccharide (LPS)-binding protein is carried on lipoproteins and acts as a cofactor in the neutralization of LPS. Antagonistic effects of lipopolysaccharide binding protein and bactericidal/permeability-increasing protein on lipopolysaccharide-induced cytokine release by mononuclear phagocytes: competition for binding to lipopolysaccharide. www.bloodjournal.org /cgi/content/full/98/13/3800   (6725 words)

Transport of Bacterial Lipopolysaccharide to the Golgi Apparatus -- Thieblemont and Wright 190 (4): 523 -- The Journal ...   (Site not responding. Last check: 2007-10-08) Transport of Bacterial Lipopolysaccharide to the Golgi Apparatus Thieblemont, N., Wright, S.D. (1997) Mice genetically hyporesponsive to lipopolysaccharide (LPS) exhibit a defect in endocytic uptake of LPS and ceramide. Munford, R.S., Hall, C.L. (1985) Uptake and deacylation of bacterial lipopolysaccharides by macrophages from normal and endotoxin-hyporesponsive mice. www.jem.org /cgi/content/full/190/4/523   (6159 words)

Structure and function of lipopolysaccharide binding protein -- Schumann et al. 249 (4975): 1429 -- Science   (Site not responding. Last check: 2007-10-08) The primary structure of lipopolysaccharide binding protein (LBP), a trace plasma protein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPSs), was deduced by sequencing cloned complementary DNA. Lipopolysaccharide from enterohemorrhagic Escherichia coli binds to platelets through TLR4 and CD62 and is detected on circulating platelets in patients with hemolytic uremic syndrome. Lipopolysaccharide induces expression of fibronectin {alpha}5{beta}1-integrin receptors in human monocytic cells in a protein kinase C-dependent fashion. www.sciencemag.org /cgi/lookup?vol=249&fp=1429&view=abstract   (2873 words)

Down-Modulation of L-Selectin by Lipopolysaccharide Is Not Required for Lipopolysaccharide-Induced Expression of CD14 ... Functional lipopolysaccharide receptors of low affinity are constitutively expressed on mouse bone marrow cells. L-selectin: a novel receptor for lipopolysaccharide and its potential role in bacterial sepsis. Lipopolysaccharide and the glycoside ring of staurosporine induce CD14 expression on bone marrow granulocytes by different mechanisms. iai.asm.org /cgi/content/full/69/7/4287   (4185 words)

Lipopolysaccharide Modeling   (Site not responding. Last check: 2007-10-08) The outer leaflet of the outer membrane of Gram-negative bacteria is composed primarily of lipopolysaccharides (LPS). The polysaccharide unit consists of the core oligosaccharide and the O-specific chain. Lipopolysaccharides are essential for the survival of Gram-negative bacteria, providing a permeation barrier for harmful substances. www.emsl.pnl.gov /docs/cbbb/modeling/biogeo/lpsSt_text.html   (284 words)

Scavenger-receptor-mediated endocytosis of lipopolysaccharide in Atlantic cod (Gadus morhua L.) -- Seternes et al. 204 ... Scavenger-receptor-mediated endocytosis of lipopolysaccharide in Atlantic cod (Gadus morhua L.) -- Seternes et al. Fluorescence micrograph of atrial endocardial endothelial cells cultured on a glass coverslip (pooled cells from three fish) and incubated with fluorescein-labelled lipopolysaccharide for 1 h at 12°C. All endocardial cells in the monolayer cultures accumulated the probe. Shnyra, A. and Lindberg, A. Scavenger receptor pathway for lipopolysaccharide binding to Kupffer and endothelial liver cells in vitro. jeb.biologists.org /cgi/content/full/204/23/4055   (5253 words)

Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1) -- ... Valvano, M. and Crosa, J. Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O7 lipopolysaccharide antigen of a human invasive strain of E. Valvano, M. and Marolda, C. Relatedness of O-specific lipopolysaccharide side chain genes from strains of Shigella boydii type 12 belonging to two clonal groups and from Escherichia coli O7:K1. Yao, Z. and Valvano, M. Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a. mic.sgmjournals.org /cgi/content/full/145/9/2485   (5317 words)

Lipopolysaccharide pretreatment attenuates myocardial infarct size: A possible mechanism involving heat shock protein ... Lipopolysaccharide pretreatment attenuates myocardial infarct size: A possible mechanism involving heat shock protein 70-inhibitory {kappa}B{alpha} complex and attenuation of nuclear factor {kappa}B -- Shimizu et al. Lipopolysaccharide pretreatment attenuates myocardial infarct size: A possible mechanism involving heat shock protein 70-inhibitory of the 2 proteins was observed in the lipopolysaccharide group. jtcs.ctsnetjournals.org /cgi/content/abstract/124/5/933   (407 words)

Cementoblast Gene Expression is Regulated by Porphyromonas gingivalis Lipopolysaccharide Partially via Toll-like ... Porphyromonas gingivalis lipopolysaccharide antagonizes Escherichia coli lipopolysaccharide at toll-like receptor 4 in human endothelial cells. Porphyromonas gingivalis lipopolysaccharide is both agonist and antagonist for p38 mitogen-activated protein kinase activation. Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via toll-like receptors. jdr.iadrjournals.org /cgi/content/full/83/8/602   (3060 words)

Ethyl Pyruvate Inhibits Nuclear Factor-{kappa}B-Dependent Signaling by Directly Targeting p65 -- Han et al. 312 (3): ... Twenty-four hours later, the cells were pretreated for 30 min with the indicated concentrations of ethyl pyruvate and then incubated in the absence or presence of lipopolysaccharide (100 ng/ml) for 30 min. For the gel depicted in A, the cells were preincubated in the absence or presence of graded concentrations of ethyl pyruvate for 1 h and then incubated in the absence or presence of lipopolysaccharide (100 ng/ml) for 15 min. The cells were incubated with or without lipopolysaccharide (100 ng/ml) in the presence of the indicated concentrations of ethyl pyruvate. jpet.aspetjournals.org /cgi/content/full/312/3/1097   (5752 words)

Lipopolysaccharide deacylation by an endogenous lipase controls innate antibody responses to Gram-negative bacteria - ...   (Site not responding. Last check: 2007-10-08) T cell−independent type 1 agonists such as Gram-negative bacterial lipopolysaccharides can stimulate B lymphocytes to proliferate and produce antibodies by signaling through Toll-like receptors. Deacylation of lipopolysaccharide occurred over several days, allowing lipopolysaccharide to act as an innate immune stimulant yet limiting the eventual amount of B cell proliferation and polyclonal antibody production. Control of lipopolysaccharide activation by acyloxyacyl hydrolase indicates that mammals can regulate immune responses to bacterial infection by chemical modification of a Toll-like receptor agonist. www.nature.com /ni/journal/v6/n10/abs/ni1246.html   (274 words)

Lipopolysaccharide from Nonvirulent Ara+ Burkholderia pseudomallei Isolates Is Immunologically Indistinguishable from ... Lipopolysaccharide from Nonvirulent Ara+ Burkholderia pseudomallei Isolates Is Immunologically Indistinguishable from Lipopolysaccharide from Virulent Ara- Clinical Isolates -- Anuntagool et al. Extraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei. Rapid serological analysis of bacterial lipopolysaccharides by electrotransfer to nitrocellulose. cdli.asm.org /cgi/content/full/5/2/225   (2642 words)

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