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Topic: Liposome transfection

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	Transfection - Wikipedia, the free encyclopedia
		Transfection differs from transformation since the DNA is not generally incorporated into the cells genome, it is only transiently expressed.
		Since the DNA introduced in the transfection process is usually not inserted into the nuclear genome, the foreign DNA is lost at the latest when the cells undergo mitosis.
		If it is desired that the transfected gene actually remains in the genome of the cell and its daughter cells, a stable transfection must occur.
	www.wikipedia.org /wiki/Transfection (578 words)

	- US Patent (Site not responding. Last check: 2007-11-06)
		In a preferred embodiment, the plasmid-liposome complex is for use in transfecting a host cell in the lung of a subject with a DNA plasmid containing cystic fibrosis transmembrane conductance regulator or, for lung carcinomas, cytokines, such as interleukin-2, or tumor suppressor genes, such as p53.
		The ratio of plasmid/histone H1 ratio was 0.3 or 0.5 and the liposome lipid/plasmid ratio was varied from 8, 14 and 18 nmoles lipid/Âµg plasmid.
		Plasmid-liposome complexes having a liposome lipid/plasmid ratio of 14 nmole lipid/Âµg plasmid was prepared by diluting 280 Âµl of the liposome suspending with 350 Âµl of distilled water and then adding 70 Âµl of 50% glucose.
	www.patentstorm.us /patents/6133026.html (7540 words)

	Patent 6245427: Non-ligand polypeptide and liposome complexes as intracellular delivery vehicles
		Indeed, the ternary complexes of cationic liposomes, DNA, and HSA, not only lead to high levels of transfection, but also have the advantages of being active in the presence of serum and, because HSA is already ubiquitous at high concentrations in tissue culture media as well as in vivo, being non-toxic.
		To investigate whether the enhancement of transfection mediated by the association of HSA or the fusogenic peptides was due to an increase in the number of cells transfected or only due to an enhancement of gene expression, we evaluated the transfection efficiency, defined as the percentage of treated cells that express the transgene.
		The liposomes were complexed, in the presence or absence of 32.mu.g of HSA with 1.mu.g of pCMVluc at the indicated theoretical lipid/DNA charge ratios.
	freepatentsonline.com /6245427.html (7275 words)

	Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity -- ...
		Transfection of established cell lines with cationic liposomes is a relatively simple and standard laboratory procedure.
		S-DNA with cationic liposomes at a DNA-to-lipid ratio of 1:15 for 6 h.
		of liposomes at a DNA-to-lipid ratio of 1:15 for 24 h.
	ajplung.physiology.org /cgi/content/full/275/3/L452 (4776 words)

	Patent 5651981: Cationic phospholipids for transfection (Site not responding. Last check: 2007-11-06)
		This invention also relates to novel liposomes and aggregates comprising the phospholipids of the present invention that are useful for the delivery of nucleic acids and drugs to cells, both in vitro and in vivo.
		For transfection, the aqueous phase is typically distilled water and the suspension is sonicated until nearly clear, which requires some minutes depending upon conditions, kind, and quality of the sonicator.
		If large liposomes are desired, the suspension is merely agitated by hand or on a vortex mixer until a uniform dispersion is obtained, i.e., until visually observable large particles are absent.
	www.freepatentsonline.com /5651981.html (7146 words)

	Science Fair Projects - Transfection
		Several different transfection techniques are available, including calcium phosphate transfection, liposome transfection, electroporation, heat shock, and proprietary transfection reagents such as Fugene.
		The choice of transfection methods differs upon cell sensitivity to the conditions, and the amount of work and cost involved.
		Typically, transfection of bacteria is done by heat shock or electroporation, which are quick, cheap and easy procedures to carry out, as bacteria are easy to grow and multiply rapidly, even from a small portion of survivors.
	www.all-science-fair-projects.com /science_fair_projects_encyclopedia/Transfection (360 words)

	Molecular biology (Site not responding. Last check: 2007-11-06)
		Introducing DNA into bacterial cells is called transformation, and can be effected by several methods, including electroporation, microinjection and chemically.
		Introducing DNA into eukaryotic cells, such as animal cells, is called transfection.
		Several different transfection technqiues are available, including calcium phosphate transfection, liposome transfection, and proprietary transfection reagents such as Fugene.
	www.sciencedaily.com /encyclopedia/molecular_biology (1333 words)

	Liposome-mediated high-efficiency transfection of human endothelial cells. (Site not responding. Last check: 2007-11-06)
		Liposome-mediated high-efficiency transfection of human endothelial cells.Liposome-mediated transfection of endothelial cells provides a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies.
		To study transfection rates, HUVEC were transfected with the pGL3 vector, containing the luciferase reporter gene, complexed with several currently available liposomes, such as different Perfect Lipid (pFx) mixtures, DMRIE-C, or lipofectin.
		The optimal transfection rate was achieved in HUVEC transfected for 1.5 h with 5 microg/ml of DNA plasmid in the presence of 36 microg/ml of pFx-7.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/8795961.html (225 words)

	Transfection Definition / Transfection Research (Site not responding. Last check: 2007-11-06)
		Transfection typically involves opening transient "holes" or gates in cells to allow the entry of extracellular molecules, typically supercoiled plasmid DNA, but additionally siRNA, among others.
		Several different transfection techniques are available, including calcium phosphate transfection, liposome transfectionLipofection (or liposome transfection) is a technique used to inject genetic material into a cell by means of liposomes which are vesicles that can easily merge with the cell membrane since they are both made of a phospholipid bilayer....
		transfection is a technique used to inject genetic material into a cell by means of liposomes which are vesicles that.
	www.elresearch.com /Transfection (411 words)

	abstract book
		The research laboratories where this invention occurred are interested in the development of non-viral vector systems to transfect cDNA for neurotrophins into the central nervous system for treatment of traumatic brain injury, spinal cord injury and other central nervous system diseases.
		The research is focused on the use of liposome-mediated neurotrophin gene transfection.
		Moreover, we have successfully transfected a report gene as well as neurotrophin genes into the uninjured and injured rat brain.
	www.uth.tmc.edu /otm/tech/bio/neurotransfect.htm (147 words)

	BioMed Central \| Full text \| Insulin receptor activation and down-regulation by cationic lipid transfection reagents
		Transfection agents comprised of cationic lipid preparations are widely used to transfect cell lines in culture with specific recombinant complementary DNA molecules.
		Widely-used cationic lipid transfection reagents cause a state of insulin unresponsiveness in cells in culture due to fully activating and subsequently reducing the expression of the receptor in cells.
		Thus, it appears that cationic liposome transfection reagents induce a state of insulin insensitivity in cultured cells through the maximal activation of the receptor followed by its subsequent down-regulation.
	www.biomedcentral.com /fulltext/pubmed/14741056 (3492 words)

	JYI Volume Five Features: Gene Therapy: Techniques of Cell Transfection
		Viral transfection is the method that most often comes to mind when many people think of gene therapy, because it is the one most often in the news.
		One explanation for poor transfection efficiency could be the patient's immune responses against the cationic lipids, while another could be the fact that many cationic vectors are unstable in the presence of serum.
		Many transfection systems, both viral and non-viral, seem promising in the lab, yet fail when introduced into humans, so the enormous number of DNA transportation systems that are being tested today may yield only a few viable treatments for gene therapy.
	www.jyi.org /volumes/volume5/issue5/features/lovejoy.html (1143 words)

	Nat' Academies Press, (NAS Colloquium) Genetic Engineering of Viruses and Viral Vectors (1996) (Site not responding. Last check: 2007-11-06)
		Although liposomes themselves have no receptors for the virus, a direct interaction of F1 polypeptide with lipid is likely to play an important role in the ability of HVJ particles to fuse with liposomes (11).
		The fusigenic viral liposome vector has proven to be efficient for the intracellular introduction of oligodeoxynucleotide, as well as intact genes up to 100 kbp, both in vitro and in vivo.
		In addition, in forming fusigenic liposome complexes, purified or recombinant fusion polypeptides may be used instead of the entire viral envelope.
	books.nap.edu /books/0309058368/html/11421.html (5346 words)

	Patent 5695780: Cationic phospholipids for transfection (Site not responding. Last check: 2007-11-06)
		It has been unexpectedly found that liposomes comprising one or more of these cationic phospholipids are effective in the lipofection of nucleic lacids.
		A cationic phospholipid according to claim 1 that is the phosphotriester derivative of a sphingomyelin.
		For some applications, it may be most effective to generate extruded liposomes by forming the suspension through a filter with pores of 100 nm diameter or smaller.
	www.freepatentsonline.com /5695780.html (6816 words)

	Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity -- ...
		Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity -- Tanswell et al.
		Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity
		Transfection of lung cells in vitro and in vivo: effect of antioxidants and intraliposomal bFGF
	ajplung.physiology.org /cgi/content/abstract/275/3/L452 (318 words)

	Gene Therapy for Attenuating Cardiac Allograft Arteriopathy Using Ex Vivo E2F Decoy Transfection by HVJ-AVE-Liposome ...
		E2F decoy or antisense cdk2 kinase ODN was transfected
		Expression of c-myb, cdc2, and cdk2 was strongly and diffusely enhanced in the thickened intima of allograft arteries from recipients without ODN transfection, whereas the expression was suppressed in the allografts transfected with E2F decoy.
		PCNA and cdc2 kinase were strongly and diffusely expressed in the thickened intima of the allograft arteries from recipients without decoy transfection, whereas their expression was weak in the allografts transfected with the E2F decoy.
	www.circresaha.org /cgi/content/full/87/11/1063 (3512 words)

	Liposome (Site not responding. Last check: 2007-11-06)
		Liposomes achieve lower transfection efficiency, but are considered safer and therefore more suited to delivery into delicate CNS tissues.
		Transfection efficiency was determined by quantifying b-galactosidase activity in cell lysates.
		A non-toxic liposomal vector was produced by reconstitution of naturally-derived lipids.
	som.flinders.edu.au /FUSA/HUMANPHYS/Neurotrophic/liposome.htm (234 words)

	CLONTECHniques Archives- July 1996: CLONfectin (TM) Transfection Reagent (Site not responding. Last check: 2007-11-06)
		Transfection with the other lipids were performed using an 8-hr incubation, according to each of the manufacturers' instructions.
		In each transfection, the SEAP activity was at least 2-3 orders of magnitude higher in cells transfected with the control vector than with mock-transfected cells.
		Several transfection methods are currently in use for introducing plasmid DNA into cultured cells, including calcium phosphate (3), cationic lipids (4), DEAE-dextran (5), electroporation (6, 7), and microinjection (8).
	www.clontech.com /archive/JUL96UPD/CLONfectin.shtml (743 words)

	Cationic Liposome-Mediated RNA Transfection -- Malone et al. 86 (16): 6077 -- Proceedings of the National Academy of ...
		Transfection of 10 ng to 5 µ g of Photinus pyralis luciferase mRNA synthesized in vitro into NIH 3T3 mouse cells yields a linear response of luciferase activity.
		Using the RNA/lipofectin transfection procedure, we have analyzed the role of capping and ß -globin 5' and 3' untranslated sequences on the translation efficiency of luciferase RNA synthesized in vitro.
		Optimal transfection with the HK polymer depends on its degree of branching and the pH of endocytic vesicles
	www.pnas.org /cgi/content/abstract/86/16/6077 (633 words)

	Abstract for TransFast(TM) Transfection Reagent
		Liposomes are one of a growing number of chemical reagents with the ability to deliver nucleic acids to eukaryotic cells by a nonviral process referred to as "transfection".
		Liposome reagents specifically designed for transfection applications incorporate synthetic cationic lipids, often formulated with the neutral lipid L-dioleoyl phosphatidylethanolamine (DOPE), which has been demonstrated to enhance the gene transfer ability of certain synthetic cationic lipids.
		The TransFast™ Transfection Reagent is designed for nucleic acid delivery to eukaryotic cells in vitro and in vivo and performs well with many cell lines.
	www.promega.com /tbs/Tb260/TB260.html (223 words)

	Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome-DNA complexes -- Torchilin et al. 100 (4): ...
		Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome-DNA complexes -- Torchilin et al.
		h, the degradation of liposomes was observed (diffuse orange/red
		The final aim of this study was to attempt an in vivo transfection in a whole-animal model with LLC cells.
	www.pnas.org /cgi/content/full/100/4/1972 (3861 words)

	Loss of Binding and Entry of Liposome-DNA Complexes Decreases Transfection Efficiency in Differentiated Airway ...
		liposomes was utilized for the studies within 12 h.
		48 h after transfection, the cells were stained with X-gal (Sigma) solution as described previously (18).
		Transfection efficiency must be increased before cationic liposomes become practical vectors for in vivo gene therapy of CF lung disease.
	www.jbc.org /cgi/content/full/272/2/1117 (5616 words)

	In Vivo Tumor Transfection with Superantigen plus Cytokine Genes Induces Tumor Regression and Prolongs Survival in Dogs ...
		Direct in vivo transfection of tumors is an alternative strategy that offers several advantages.
		in a 1:1 ratio (micrograms of DNA to nanomoles of liposome) (34).
		Yang (1996) Gene gun-mediated skin transfection with interleukin-12 gene results in regression of established primary and metastatic murine tumors.
	www.jci.org /cgi/content/full/101/11/2406 (6401 words)

	IngentaConnect Stearylamine Liposome as a New Efficient Reagent for DNA Transfec... (Site not responding. Last check: 2007-11-06)
		The efficiency of stearylamine (SA) liposome in transfecting DNA into eukarytic cells was evaluated and compared with that of the Lipofectin reagent and the calcium phosphate transfection method.
		The overall indication was that SA liposomes had certain advantages over the Lipofectin reagent and both of them were superior to the calcium phosphate method, showing the greatest effect in 8 different cell lines, while the calcium phosphate method was the best in only 4 cell lines.
		The number of cell lines failed to be transfected was 2 for SA liposomes, 3 for calcium phosphate method, and 4 for the Lipofectin reagent.
	api.ingentaconnect.com /content/ap/rc/1996/00000226/00000002/art01376 (272 words)

	Liposome mediated gene transfer into the rat oesophagus -- Schmid et al. 41 (4): 549 -- Gut
		In the present study liposomes were used to complex with DNA to transfer a recombinant gene into cells in vivo.
		In vivo transfection of murine lungs with a functioning prokaryotic gene using liposome vehicle.
		Nicolau C, Legrand A, Grosse E. Liposomes as carriers for in vivo gene transfer and expression.
	gut.bmjjournals.com /cgi/content/full/41/4/549 (4023 words)

	HLA Class II Expression in Uninducible Hepatocarcinoma Cells After Transfection of AIR-1 Gene Product CIITA: ...
		Transfection of an expressible CIITA cDNA in Alexander cells
		Cells were incubated with mAbs specific for the human MHC class II Ags listed in the top of the histograms, followed by staining with the rabbit anti-mouse FITC.
		As control, specific transcripts of the the housekeeping gene ß-actin amplified from the same RNA preparations of the various cells are shown.
	www.jimmunol.org /cgi/content/full/161/2/814 (5402 words)

	Immunoliposome-Mediated Gene Transfer into Cultured Myotubes (Site not responding. Last check: 2007-11-06)
		The immunoliposomes conjugated with the antibody specific for myotubes were three times as efficient as control immunoliposomes conjugeted with an antibody not specific for myotubes.
		However, the efficiency was no more than that by the cationic liposomes without the antibody.
		These results suggest that laminin B2 is not effective in enhancing the efficiency of gene transfection for non-proliferating myotubes.
	journal.med.tohoku.ac.jp /1923/192302.html (150 words)

	Transfection reagent for improved gene silencing: News from Cambridge BioScience
		Transfection reagent for improved gene silencing: News from Cambridge BioScience
		New transfection reagent TransIT-Siquest is said to be ideal for siRNA-mediated gene silencing studies in mammalian cells.
		Developed by Mirus and available from Cambridge BioScience, the unique lipopolyplex formulation enables highly efficient siRNA transfection with significantly reduced levels of cell damage compared to cationic-liposome based transfection reagents.
	www.laboratorytalk.com /news/cmr/cmr149.html (224 words)

	Gene Delivery for Metastatic Prostate Cancer Cells - Storming Media
		Using liposome-mediate DNA transfection, the vector specifically destroys prostate cancer cells.
		Enhanced by the by-stander effect, the specific expression of the diphtheria toxin A gene causes significant cell death in prostate cancer cell culture, with very low background in the control cell lines.
		We have developed a prostate-specific vector to deliver the diphtheria toxin A gene into cancer cells.
	www.stormingmedia.us /32/3245/A324583.html (200 words)

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