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Topic: Lipoxygenase

In the News (Thu 18 Oct 18)

  The Selenoenzyme Phospholipid Hydroperoxide Glutathione Peroxidase Controls the Activity of the 15-Lipoxygenase with ...
One general feature of the lipoxygenase reaction is the requirement for small amounts of hydroperoxy lipids acting as essential activators of the enzyme(19, 20).
The lipoxygenase activity with complex substrates was measured oxygraphically in a 1.6-ml oxygraphic chamber at 25 °C. Unless stated otherwise, the incubation mixture consisted of 0.1 M Tris-HCl buffer, pH 7.4, containing either submitochondrial particles (1 mg of protein/ml) or human LDL (0.5 mg of protein/ml) as substrate.
Lipoxygenases are known to require small amounts of hydroperoxy fatty acids as activators for their catalytic action(19, 20).
www.jbc.org /cgi/content/full/271/9/4653   (4429 words)

Lipoxygenases are mononuclear iron enzymes that catalyse the dioxygenation of polyunsaturated fatty acids in reaction (1):
Lipoxygenases are found in plants and in animals [1-3].
The plant lipoxygenases can be enzymatically cleaved into two fragments which stay tightly associated while the enzyme remains active; separation of the two domains leads to loss of catalytic activity [7].
metallo.scripps.edu /PROMISE/LIPOXYGENASE.html   (632 words)

 Structural studies of lipoxygenases
Finazzi­Agro, A., Avigliano, L., Veldink, G.A., Vliegenthart, J.F.G. and Boldingh, J. The influence of oxygen on the fluorescence of lipoxygenase.
Nelson, M.J., Batt, D.G., Thompson, J.S. and Wright, S.W. Reduction of the active­site iron by potent inhibitors of lipoxygenases.
Nelson, M.J., Chase, D.B. and Seitz, S.P. a) Photolysis of "purple" lipoxygenase: implications for the structure of the chromophore.
metallo.scripps.edu /promise/LIPOXYGENASE_REF.html   (1538 words)

 InterPro: IPR000907 Lipoxygenase
Lipoxygenases (EC:1.13.11.-) are a class of iron-containing dioxygenases which catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4- pentadiene structure.
In mammals a number of lipoxygenases isozymes are involved in the metabolism of prostaglandins and leukotrienes [ 2 ].
The iron atom in lipoxygenases is bound by four ligands, three of which are histidine residues [ 4 ].
www.ebi.ac.uk /interpro/IEntry?ac=IPR000907   (3208 words)

 UCSC Biomedical Research Faculty - Professor Ted Holman
Lipoxygenases are enzymes implicated in a broad range of human cancers, as well as cardiac and inflammatory diseases.
These lipoxygenase products are the precursors of hormones, such as leukotrienes and lipoxins, which have been implicated as critical signaling molecules in a variety of inflammatory diseases and cancers.
Holman believes that the allosteric site may be critical to the regulation of lipoxygenase activity by either suppressing or activating the enzyme, and that the site might serve as a potential target for a novel class of lipoxygenase inhibitors.
biomedical.ucsc.edu /Holman.html   (655 words)

 Lipoxygenase - Wikipedia, the free encyclopedia
Lipoxygenases are iron-containing enzymes that catalyse the dioxygenation of polyunsaturated fatty acids in reaction:
The plant lipoxygenases can be enzymatically cleaved into two fragments which stay tightly associated while the enzyme remains active; separation of the two domains leads to loss of catalytic activity.
Kulkarni, A.P. "Lipoxygenase - a versatile biocatalyst for biotransformation of endobiotics and xenobiotics".
en.wikipedia.org /wiki/Lipoxygenase   (747 words)

 Lipoxygenase inhibitors as treatments of pancreatic cancer
Apoptosis of pancreatic cancer cells induced by lipoxygenase inhibitors was related to a reduction in the levels of antiapoptotic proteins Bcl-2 and Mcl-1 while that of the proapoptotic protein bax was increased.
Lipoxygenase inhibitors also markedly induced the release of cytochrome c from mitochondria into the cytosol.
Lipoxygenase inhibitors attenuate growth of human pancreatic cancer xenografts and induce apoptosis through the mitochondrial pathway.
www.bioportfolio.com /LeadDiscovery/PubMed-010326.html   (792 words)

 Soybean Lipoxygenase-1 Structure
The pictures that follows were based on coordinates of the 1.4 Å structure (1996 paper).
The first crystallographic structure reports noted a long thin cavity running through the lipoxygenase molecule.
Pathway B: Corey, E. J., and Nagata, R. "Evidence in favor of an organoiron-mediated pathway for lipoxygenation of fatty acids by soybean lipoxygenase".
www.haverford.edu /chem/Scarrow/SLO/SoyLoxStr.html   (1012 words)

 Expression of 5-lipoxygenase and 5-lipoxygenase-activating protein in human fetal membranes throughout pregnancy and at ...
Lipoxygenase metabolites may be involved in human parturition.
Bennett, P.R., Elder, M.G. and Myatt, L. (1987a) The effects of lipoxygenase metabolites of arachidonic acid on human myometrial contractility.
Saeed, S.A. and Mitchell, M.D. (1982) Formation of arachidonate lipoxygenase metabolites by human fetal membranes, uterine decidua vera, and placenta.
molehr.oxfordjournals.org /cgi/content/full/5/7/668   (3643 words)

 Human 12(R)-Lipoxygenase and the Mouse Ortholog. MOLECULAR CLONING, EXPRESSION, AND GENE CHROMOSOMAL ASSIGNMENT -- Sun ...
Lipoxygenases are a family of non-heme iron-containing dioxygenases that introduce molecular oxygen stereospecifically into
Lipoxygenase fusion proteins expressed in COS-M6, HEK 293 cells, and baculovirus-infected insect cells examined by fluorescence microscopy and immunoblot analysis.
Lanes: 1, epidermal-type 12-lipoxygenase (15); 2, mouse lipoxygenase cloned in this study; 3 and 4, two different samples of human lipoxygenase cloned in this study; 5, uninfected Sf9 insect cell control.
www.jbc.org /cgi/content/full/273/50/33540   (5086 words)

 Arachidonate dilates basilar artery by lipoxygenase-dependent mechanism and activation of K+ channels -- Faraci et al. ...
is mediated by a product(s) of the lipoxygenase pathway, with
the lipoxygenase pathway and that dilator responses of the basilar
Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate.
ajpregu.physiology.org /cgi/content/full/281/1/R246   (5456 words)

 E0051: Lipoxygenase - A Mysterious 'Purple' Form   (Site not responding. Last check: 2007-10-10)
Lipoxygenase belongs to a family of dioxygenases that catalyze oxygenation of polyunsaturated fatty acids by a redox reaction involving a non-heme iron cofactor, molecular oxygen, a diene system from a fatty acid and the enzyme's amino acids that support this process.
'Purple' lipoxygenase has been described[2] as an uncharacterized metastable state of enzyme triggered by addition of substrate and oxygen or excess product (13HPOD) to a solution of enzyme.
In this presentation we report recent results of x-ray analysis of the 'purple' form of soybean lipoxygenase-3[3] obtained by penetration of 13-HPOD into the crystals.
www.hwi.buffalo.edu /ACA/ACA99/abstracts/text/E0051.html   (180 words)

 Breeding Triple-null Lipoxygenase Soybean Cultivars
These cultivars are being designed for use in three different soyfood roles: processed soyfoods, green immature edible soybean, and fl mature edible soybean.
After selfing, seeds were tested for the absence of the three lipoxygenase enzymes.
Emerald was crossed with KY10-146, a triple lipoxygenase null line from the ‘7499’ backcrossing program.
www.uky.edu /Ag/NewCrops/soybreeding.htm   (544 words)

 Dual role of oxygen during lipoxygenase reactions -- Ivanov et al. 272 (10): 2523 -- FEBS Journal
Schilstra MJ, Veldink GA and Vliegenthart JF (1994) The dioxygenation rate in lipoxygenase catalysis is determined by the amount of iron (III) lipoxygenase in solution.
Glickman MH and Klinman JP (1996) Lipoxygenase Reaction Mechanism: Demonstration that hydrogen abstraction from substrate precedes dioxygen binding during catalytic turnover.
Purification, characterization and biological dynamics of the lipoxygenase; its identity with the respiratory inhibitors of the reticulocyte.
content.febsjournal.org /cgi/content/full/272/10/2523   (4981 words)

 Association of a Lipoxygenase Locus, Lpx-B1, with Variation in Lipoxygenase Activity in Durum Wheat Seeds -- Hessler et ...
lipoxygenase activity in seeds of a durum wheat population and
Alignment of a previously published cDNA lipoxygenase sequence (LoxA) from barley (van Mechelen et al., 1995) with four genomic lipoxygenase sequences amplified from the durum wheat varieties Jennah Khetifa and Cham 1.
Primary structure of lipoxygenase from barley grain as deduced from its cDNA sequence.
crop.scijournals.org /cgi/content/full/42/5/1695   (3802 words)

 Expression of Arachidonate 12-Lipoxygenase in Rat Tissues: A Possible Role in Glucagon Secretion -- Kawajiri et al. 48 ...
Brash AR (1999) Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate.
Funk CD (1996) The molecular biology of mammalian lipoxygenases and the quest for eicosanoid functions using lipoxygenase-deficient mice.
Nadler JL, Natarajan R, Stern N (1987) Specific action of the lipoxygenase pathway in mediating angiotensin II-induced aldosterone synthesis in isolated adrenal glomerulosa cells.
www.jhc.org /cgi/content/full/48/10/1411   (3384 words)

 Inhibition of the 5-lipoxygenase pathway - Patent 5134150
Under optimal conditions, it is likely that an agent with preferential lipoxygenase inhibitory activity would not share the ulcerogenic liability of cyclooxygenase inhibitors or the toxicity of corticosteroids.
Dermatol., 109, 126-129 (1983)], a compound with in vitro lipoxygenase inhibitory activity on psoriasis, lends support to the concept that 5-lipoxygenase pathway inhibitors can be useful in the treatment of psoriasis.
Further elucidation of the anti-inflammatory activity of Compound 1 was achieved in models of arachidonic acid-induced edema in mice and rats.
www.freepatentsonline.com /5134150.html   (14517 words)

 lipoxygenase - Faculté Universitaire des Sciences Agronomiques de Gembloux (Belgique)
- Extraction, purification and caracterisation of soybean lipoxygenase isoenzyme-1 (Lox-1)
- Study of lipoxygenase activity in in vitro cultures in relation with physilogical events (collaboration with Dr Mondher Jaziri, laboratory of plant morphology, Université Libre de Bruxelles).
- Evaluation of lipoxygenase activity in potatoes during storage.
www.fsagx.ac.be /cg/e_lox.htm   (340 words)

 UGA Soybean Genetics and Development Laboratory | Protocols
Simple and rapid method for the selective detection of individual lipoxygenase isozymes in soybean seeds.
The photobleaching rate can vary from sample to sample, so recheck some of the initially scored samples to assure that significant photobleaching hasn't occurred after a sample has been scored.
An additional check to verify results is to repeat assays on null seeds (e.g., those having none of the 3 lipoxygenase enzymes).
www.cropsoil.uga.edu /soylab/lipoxygenase.html   (521 words)

 15-Lipoxygenase: A Janus Enzyme? -- Chanez et al. 27 (6): 655 -- American Journal of Respiratory Cell and Molecular ...
Lipoxygenases (LO) are a class of nonheme iron dioxygenases
Oxygenation of biological membranes by the pure reticulocyte lipoxygenase.
A lipoxygenase in rabbit reticulocytes with attacks phospholipids and intact mitochondria.
ajrcmb.atsjournals.org /cgi/content/full/27/6/655   (2883 words)

 A Pertussis Toxin-Sensitive 8-Lipoxygenase Pathway Is Activated by a Nicotinic Acetylcholine Receptor in Aplysia ...
In the nervous system, the actions produced by lipoxygenase metabolites are cell- and neurotransmitter-specific.
The diversity of the lipoxygenase family: many sequence data but little information on biological significance.
Piomelli D, Volterra A, Dale N, Siegelbaum SA, Kandel ER, Schwartz JH, and Belardetti F. Lipoxygenase metabolites of arachidonic acid as second messengers for presynaptic inhibition of Aplysia sensory cells.
jn.physiology.org /cgi/content/full/85/5/2150   (4578 words)

 Lipoxygenase Modulation to Reverse Carcinogenesis -- Shureiqi and Lippman 61 (17): 6307 -- Cancer Research
Brash A. Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate..
Evidence for a role of the reticulocyte lipoxygenase in the maturation of red cells.
Liu B., Timar J., Howlett J., Diglio C. A., Honn K. Lipoxygenase metabolites of arachidonic and linoleic acids modulate the adhesion of tumor cells to endothelium via regulation of protein kinase C..
cancerres.aacrjournals.org /cgi/content/full/61/17/6307   (6260 words)

 A 12R-lipoxygenase in human skin: Mechanistic evidence, molecular cloning, and expression -- Boeglin et al. 95 (12): ...
for the 12R-HETE synthesis is a cytochrome P450 or a lipoxygenase.
The 12-HETE was chromatographed as the PFB ester on a Chiralcel OD column using a solvent of hexane/isopropanol (100:5, vol/vol) and detected by UV monitoring at 235 nm.
The samples were analyzed by negative ion/chemical ionization GC-MS as the PFB ester trimethylsilyl ether derivatives by repetitive scanning in the range m/z 390-404.
www.pnas.org /cgi/content/full/95/12/6744   (4578 words)

 In Vivo Action of 15-Lipoxygenase in Early Stages of Human Atherogenesis -- Kühn et al. 99 (5): 888 -- Journal of ...
suggests that the lipoxygenase may be inactive on endogenous substrates.
Simon, T.C.,A.N. Makheja, andJ.M. Bailey(1989)The induced lipoxygenase in atherosclerotic aorta converts linoleic acid to the platelet chemorepellant factor 13-HODE.
Chan(1995)Transgenic rabbits with the integrated human 15-lipoxygenase gene driven by a lysozyme promotor: macrophage-specific expression and variable positional specificity of the transgenic enzyme.
www.jci.org /cgi/content/full/99/5/888   (4535 words)

 Cyclooxygenase- and lipoxygenase-dependent relaxation to arachidonic acid in rabbit small mesenteric arteries -- Zhang ...
For sodium nitroprusside responses, arteries were pretreated with indomethacin (10 µM) or the lipoxygenase inhibitor cinnamyl-3,4-dihydroxy-cyanocinnamate (CDC; 1 µM).
Arteries were pretreated with the lipoxygenase inhibitors CDC (1 µM; A), nordihydroguaiaretic acid (NDGA; 1 µM) or vehicle (B), or ebselen (1 µM; C) in the presence of indomethacin (10 µM).
C]AA and examined the lipoxygenase metabolites by reverse-phase HPLC.
ajpheart.physiology.org /cgi/content/full/288/1/H302   (3744 words)

 E0055: Soybean Lipoxygenase-3, Complexes with Small Molecules   (Site not responding. Last check: 2007-10-10)
Studies of the structure and properties of lipoxygenase enzymes are of great interest due to their relation in humans to the inflammatory responses and various diseases.
The structures of rabbit and soybean enzymes have similar organization and orientation of major domains and share similar structure of the active site with one non-heme iron cofactor.
Those facts support our persistence in studying easier to obtain plant en-zyme, and yet providing valuable insights into the features of lipoxygenases in general, such as ligand binding and possible mechanism of action.
www.hwi.buffalo.edu /ACA/ACA98/abstracts/text/E0055.html   (333 words)

 5-Lipoxygenase and Leukotrienes . Transgenic Mouse and Nuclear Targeting Studies -- FUNK and CHEN 161 (2): 120 -- ...
decided to adopt this approach for the study of lipoxygenase enzymes.
Funk, C. The molecular biology of mammalian lipoxygenases and the quest for eicosanoid functions using lipoxygenase-deficient mice.
A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis.
ajrccm.atsjournals.org /cgi/content/full/161/2/S1/S120   (2372 words)

 Arachidonic Acid-Induced Vasodilation of Rat Small Mesenteric Arteries Is Lipoxygenase-Dependent -- Miller et al. 304 ...
rat mesenteric arteries that is abolished by inhibitors of lipoxygenase,
Soberman RJ, Harper TJ, Betteridge D, Lewis RA and Austen KF (1985) Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.
Uski TK and Hogestatt ED (1992) Effects of various cyclooxygenase and lipoxygenase metabolites on guinea-pig cerebral arteries.
jpet.aspetjournals.org /cgi/content/full/304/1/139   (3751 words)

The released AA can be metabolized by either the lipoxygenase pathway where AA is converted by fatty acid lipoxygenases into hydroperoxy derivatives, giving rise to leukotrienes, or the cycloxygenase pathway where a fatty acid transforms AA rapidly to the prostaglandin PGG21 which in turn is further converted enzymatically.
    In general the present invention relates to the use of lipoxygenase inhibitors in therapeutic applications, in particular to the prevention and treatment of epithelial cell-derived cancers.
Use of lipoxygenase inhibitors and PPAR ligands as anti-cancer therapeutic and intervention agents
www.delphion.com /details?pn=WO09524894A2   (601 words)

 Amazon.com: lipoxygenase   (Site not responding. Last check: 2007-10-10)
Lipoxygenase and Lipoxygenase Pathway Enzymes by George Piazza (Hardcover - Jun 30, 1996)
Lipoxygenases and Their Metabolites: Biological Functions (Advances in Experimental Medicine and Biology) by Santosh Nigam and Cecil R. Pace-Asciak (Hardcover - Jan 31, 1999)
Modification of the lipoxygenase reaction by wheat glutenin by Aris Graveland (Unknown Binding - 1971)
www.amazon.com /s?ie=UTF8&keywords=lipoxygenase&tag=kalunguelesea-20&index=blended&link_code=qs&page=1   (548 words)

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