
	Where results make sense

Topic: Locked nucleic acid

Related Topics

amino acid

nucleic acid

fatty acid

hydrochloric acid

nitric acid

sulfuric acid

The Acid House

acetic acid

carboxylic acid

citric acid cycle

lactic acid

acid rain

In the News (Fri 17 Feb 12)

EXECUTIVE POWER

Iran

ANALYSIS

Pakistan

Family compact

	Locked nucleic acid - Wikipedia, the free encyclopedia
		A locked nucleic acid (LNA) is a modified RNA nucleotide.
		LNA increases the thermal stability (melting temperature) of oligonucleotides.
		DNA - RNA - LNA - PNA - mRNA - ncRNA - miRNA - rRNA - shRNA - siRNA - tRNA - mtDNA - Oligonucleotide
	en.wikipedia.org /wiki/Locked_nucleic_acid (255 words)

	Eurogentec
		LNA is a novel proprietary nucleic acid analogue, which is easily incorporated in synthetic DNA oligonucleotides thereby providing unprecedented sensitivity, specificity and discriminatory power to a wide range of genomics products.
		LNA containing oligonucleotides will be synthesised in Oswel, our UK facility, a 100% subsidiary of Eurogentec and will be distributed throughout the Eurogentec world-wide sales and distribution network." Eurogentec quote.
		LNA provides significantly improved sensitivity, specificity and discriminatory power to nucleic acid recognition assays in a format fully compatible with current molecular biology protocols.
	www.eurogentec.com /code/EN/Pres_rele.asp?id_presse=61 (678 words)

	LNA (Locked Nucleic Acid) Microarrays - A New Platform for Gene Expression Profiling (Site not responding. Last check: 2007-08-05)
		LNAs (locked nucleic acids) constitute a novel class of bicyclic DNA analogues that have an exceptionally high affinity toward complementary DNA and RNA due to increased thermal stability of the heteroduplexes.
		We have chosen a subset of genes for the use in LNA microarrays to monitor heat-shock responses in the yeast Saccharomyces cerevisiae.
		Technical aspects of the LNA microarray platform as well as data obtained with the yeast and C.
	www.ornl.gov /sci/techresources/meetings/transcriptome2002/p25.shtml (202 words)

	lna (Site not responding. Last check: 2007-08-05)
		LNA is a bicyclic nucleotide containing a 2’-O,4’-methylene-bridge.
		The consequence of the methylene-bridge is that the nucleotide is locked in a C3’-endo conformation, this makes LNA a potential anti sense molecule, knowing that RNA also have C3’-endo conformation, whereas DNA have a C2’-endo conformation.
		Even though the LNA nucleotide is locked in a C3’-endo conformation, it has shown remarkable good hybridations to both DNA and RNA, indicating that the stability of a duplex is influenced by other factors than the sucker conformation.
	virgil.ruc.dk /~cbangn/christina/lna.html (237 words)

	Locked nucleic acid Definition / Locked nucleic acid Research (Site not responding. Last check: 2007-08-05)
		A locked nucleic acid (LNA) is a modified RNARibonucleic acid (RNA) is a nucleic acid consisting of a string of covalently-bound nucleotides.
		Locked Nucleic Acid is a novel chemical DNA analog, that can be substituted in DNA and RNA.
		Locked nucleic acid is a promising and powerful technology for real-time quantitative polymerase chain reaction.
	www.elresearch.com /Locked_nucleic_acid (216 words)

	Joacim Elmén: Doktorsavhandling från Karolinska Institutet
		Nucleic acid therapy comprises several approaches based on nucleic acid as the active therapeutic component to treat human disease.
		Based on nucleic acid sequence recognition both techniques inhibit gene expression.
		Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality.
	diss.kib.ki.se /2005/91-7140-047-8 (581 words)

	Profiling PCR and Beyond - Tenth Annual Nucleic Acid-Based Technologies
		Nucleic acid amplification and detection have become the most widely used technique for conducting biological research.
		New technical developments that improve the performance of nucleic acid amplification and detection, as well as interesting examples of how these techniques are being used, are the emphases of this meeting.
		Locked nucleic acids (LNA) are conformationally restricted oligonucleotide analogues that form extremely strong duplexes with their cognate targets.
	www.healthtech.com /2002/pcr (5006 words)

	Locked nucleic acid (LNA) as cancer-therapeutic agent
		The aim of this basic research project has been to investigate the utility of locked nucleic acid (LNA) as antisense molecule and the potential of LNA to inhibit the growth of cancer cells.
		LNA is a novel class of nucleic acid analogues, which exhibits unprecedented thermal stability towards complementary LNA, DNA and RNA.
		This study is a basic research project, in which the ability of a new nucleic acid analogue has been examined for its antisense properties in cancer cells.
	www.danmedbul.dk /DMB_2004/0104/0104-phd/DMB3584.htm (737 words)

	Glen Research Glen Report
		LNA is a bicyclic nucleic acid where a ribonucleoside is linked between the 2’-oxygen and the 4’-carbon atoms with a methylene unit.
		In fact, LNA is recommended for use in any hybridization assay that requires high specificity and/or reproducibility, e.g., dual labelled probes, in situ hybridization probes, molecular beacons and PCR primers.
		LNA can be mixed with DNA and RNA, as well as other nucleic acid analogues, modifiers and labels.
	www.glenres.com /GlenReports/GR16-24.html (578 words)

	World Intellectual Property Organization (Site not responding. Last check: 2007-08-05)
		An LNA phosphoramidite for direct use in oligonucleotide synthesis may be obtained directly or by simple precipitation of the product from the reaction mixture.
		The molar ratio between the LNA monomer and the nucleophilic activator is typically in the range of 1: 0.0001 to 1: 10, preferably 1: 0.0001 to 1: 1, and more preferably 1: 0.0001 to 1: 0.7.
		The method according to any of the preceding claims, wherein the molar ratio between the LNA monomer and the nucleophilic activator is in the range of 1: 0.0001 to 1: 10.
	www.wipo.int /ipdl/IPDL-CIMAGES/view/pct/getbykey5?KEY=03/06475.030123&ELEMENT_SET=DECL (2040 words)

	Encyclopedia: Locked nucleic acid
		Ribonucleic acid (RNA) is a nucleic acid consisting of a string of covalently-bound nucleotides.
		A nucleotide is a monomer or the structural unit of nucleotide chains forming nucleic acids as RNA and DNA.
		LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieveanddb=pubmedanddopt=Abstractandlist_uids=15491130)
	www.nationmaster.com /encyclopedia/Locked-nucleic-acid (256 words)

	OligoDesign: optimal design of LNA (locked nucleic acid) oligonucleotide capture probes for gene expression profiling ...
		The 50 gene-specific CYP450 50mer LNA oligonucleotides were spotted in quadruplicate along with Cy3 labelled marker oligonucleotides (enclosed in white boxes) onto the Immobilizer microarray slide (Exiqon, Denmark).
		Koshkin,A., Singh,S.K., Nielsen,P., Rajwanshi,V.K., Kumar,R., Meldgaard,M., Olsen,C.E., and Wengel,J. (1998) LNA (locked nucleic acids): synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition.
		Singh,S.K., Nielsen,P., Koshkin,A. and Wengel,J. (1998) LNA (locked nucleic acids): Synthesis and high-affinity nucleic acid recognition.
	nar.oxfordjournals.org /cgi/content/full/31/13/3758 (2910 words)

	Scientific Program — Fifth HIV DRP Symposium: Antiviral Drug Resistance
		Minus (-) strand DNA synthesis in retroviruses is accompanied by degradation of viral RNA of the RNA/DNA replication intermediate, events mediated by the N-terminal DNA polymerase and C-terminal ribonuclease H (RNase H) catalytic centers of the multifunctional reverse transcriptase (RT), respectively.
		Correct positioning of nucleic acid to facilitate each of these events clearly requires their specific interaction with structural motifs of the virus-coded polymerase.
		In contrast, the "unzipped" portion of the PPT was moderately insensitive to LNA template insertions.
	www.ncifcrf.gov /campus/symposium/abstract57_2004.html (347 words)

	Category:Nucleic acids - Wikipedia, the free encyclopedia
		Nucleic acids are complex, high-molecular-weight biochemical macromolecules composed of nucleotide chains.
		For more information, see the article about Nucleic acids.
		This page was last modified 19:39, 1 March 2005.
	en.wikipedia.org /wiki/Category:Nucleic_acids (46 words)

	Locked nucleic acids
		The structure and synthesis of LNA (locked nucleic acid) is presented.
		LNA is defined as an oligonucleotide containing one or more 2'-O, 4'-C-methylene-β-D-ribofuranosyl nucleotide monomer(s).
		The LNA molecular family is presented and the member classes are described in relation to their chemical structures.
	stacks.iop.org /0953-8984/15/S1861 (237 words)

	One step beyond the competition: News from Thermo Electron Laboratory Equipment
		Nucleic Acids (LNA) to the worldwide scientific research community from the Danish company Exiqon.
		Due to the enhanced stability of LNA-DNA hybrids, LNA probes are able to improve nearly all types of basic genomic research, such as Fish and microarray analysis as well as antisense applications.
		The broad application range of LNA combined with Thermo's experience in nucleic acid chemistry and biopolymer synthesis will take customers to a new edge of genomic research - one step ahead of their scientific competitors - says Thermo.
	www.laboratorytalk.com /news/thr/thr116.html (278 words)

	MB Departmental News, MBI seminar
		Nucleic Acid Center, Department of Chemistry and Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark;
		There is a big interest in developing nucleic acid analogues for various uses: antisense therapy, mimicry modeling/antagonists, tools for molecular biology, clinical diagnosis, biosensors, etc. Nucleic acid as a therapeutic tool is an attractive approach.
		Some general needs for a therapeutic nucleic acid are; small size, biological stability, high specificity and high catalytic power.
	dna8.mb.au.dk /deptnews/seminars/Fall-03/27-11-2003.htm (224 words)

	U.S. Genomics Licenses Locked Nucleic Acid (LNA (TM)) Chemistries from Exiqon
		Exiqon's LNA (TM) technology adds even greater sensitivity, specificity and discriminatory capabilities to the U.S. Genomics platform due to LNA's ultra high binding affinity, a unique advantage to target small RNA's.
		Two unique proprietary core technologies, AQ-Link (TM) and LNA (TM) form the basis of product development which in combination with a focused out-licensing and partnering strategy seeks to create increased market value and revenue for its partners and customers.
		LNA (TM) provides significantly improved sensitivity, specificity and discriminatory power to nucleic acid recognition assays in a format fully compatible with current molecular biology protocols.
	www.prnewswire.com /cgi-bin/stories.pl?ACCT=104&STORY=/www/story/04-14-2004/0002151197&EDATE= (687 words)

	Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR -- ...
		Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR -- Johnson et al.
		Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR
		Braasch,D.A. and Corey,D.R. (2001) Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA.
	nar.oxfordjournals.org /cgi/content/full/32/6/e55 (4667 words)

	IngentaConnect Use of locked nucleic acid oligonucleotides to add functionality ... (Site not responding. Last check: 2007-08-05)
		The design and ability of oligonucleotides (ODNs) containing locked nucleic acids (LNAs) to bind supercoiled, double-stranded plasmid DNA in a sequence-specific manner are described for the first time.
		The main mechanism for LNA ODNs binding plasmid DNA is demonstrated to be by strand displacement.
		LNA ODNs are more stably bound to plasmid DNA than similar peptide nucleic acid (PNA) ‘clamps’ for procedures such as particle-mediated DNA delivery (gene gun).
	www.ingentaconnect.com /content/oup/nar/2003/00000031/00000020/art05817 (304 words)

	Sequence-Dependent Thermodynamic Parameters for Locked Nucleic Acid (LNA)-DNA Duplex Formation
		The design of modified nucleic acid probes, primers, and therapeutics is improved by considering their thermodynamics.
		Locked nucleic acid (LNA) is one of the most useful modified backbones, with incorporation of a single LNA providing a substantial increase in duplex stability.
		Both the 5' and 3' neighbors must be considered in predicting the effect of an LNA incorporation, with purine neighbors providing more stability.
	www.biochem.umd.edu /biochem/kahn/abstracts/abs26.html (249 words)

	Two Modes of HIV-1 Polypurine Tract Cleavage Are Affected by Introducing Locked Nucleic Acid Analogs into the (-) DNA ...
		and act as a sensor of nucleic acid geometry (14-19).
		LNA phosphoramidites of A, G, T, and 5-methyl cytidine were purchased from Proligo Reagents.
		as a sensor of nucleic acid geometry during translocation (19).
	www.jbc.org /cgi/content/full/279/35/37095 (5260 words)

	LNA (Locked Nucleic Acid): High-Affinity Targeting of Complementary RNA and DNA (Site not responding. Last check: 2007-08-05)
		Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation.
		LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double-stranded DNA.
		The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.
	pubs.acs.org /cgi-bin/abstract.cgi/bichaw/2004/43/i42/abs/bi0485732.html (147 words)

	Use of locked nucleic acid oligonucleotides to add functionality to plasmid DNA -- Hertoghs et al. 31 (20): 5817 -- ...
		LNA is an analogue of RNA or DNA.
		Unbound LNA ODN was removed, the plasmid–LNA ODN complex was incubated with the fluorescein-labelled DNA primer KH2 (4 µM) and free ODN was again removed prior to loading on a gel (except lane 4).
		LNA binding to plasmid is only via G and A residues (Table 1).
	nar.oupjournals.org /cgi/content/full/31/20/5817 (6841 words)

	US Genomics - News & Events
		T“Incorporating the Exiqon LNA technology into our Trilogy platform will provide life science researchers with a powerful new tool to analyze and detect single molecules, particularly microRNAs,” said Stephen P. DeFalco, Chairman and CEO of U.S. Genomics.
		Exiqon’s LNA technology adds even greater sensitivity, specificity and discriminatory capabilities to the U.S. Genomics platform due to LNA’s ultra high binding affinity, a unique advantage to target small RNA’s.
		Two unique proprietary core technologies, AQ-Link and LNA form the basis of product development which in combination with a focused out-licensing and partnering strategy seeks to create increased market value and revenue for its partners and customers.
	www.usgenomics.com /news/pressreleases/041404.shtml (589 words)

	Detection of the Factor V Leiden Mutation by Direct Allele-specific Hybridization of PCR Amplicons to Photoimmobilized ...
		Nucleic Acids Res., March 26, 2004; 32(6): e55 - e55.
		Nucleic Acids Res., December 1, 2002; 30(23): 5160 - 5167.
		Nucleic Acids Res., September 1, 2002; 30(17): e91 - 91.
	www.clinchem.org /cgi/content/abstract/45/11/1898 (452 words)

	Santaris Pharma raises EUR 4.4 million in interim funding from lead investors and launches second round financing
		LNA drugs, with their high potency and biostability, have the potential to transform the field of gene-targeted therapeutics, making specific and effective gene silencing a reality in human medicine.
		If this potential is realised, even in part, it should be possible to design new drugs to treat a wide variety of human diseases by switching off the expression of harmful genes.
		The drug is composed of a short single chain of nucleotides including LNA, a conformational analogue of RNA, which confers very high specific RNA binding activity on the drug.
	www.bioportfolio.com /news/Santaris_Pharma_2.htm (945 words)

Try your search on: Qwika (all wikis)