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Topic: Luminol


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  Howstuffworks "How Luminol Works"
But one of the most outlandish TV cop tools, a special chemical that reveals invisible blood traces, is entirely real.
In this article, we'll find out how this strange compound, commonly known as luminol, reveals hidden crime scenes.
As we'll see, this chemical is just as cool as it sounds, but it does have drawbacks and limitations not usually addressed on TV.
science.howstuffworks.com /luminol.htm   (168 words)

  
  Luminol for Dummies - Ian-Albert.com
To facilitate the hemoglobin and luminol mixing you have to destroy the blood cell walls, which is another reason the forensic folks don't use luminol much—destroying cell walls tends to destroy DNA evidence, too.
Fortunately, luminol is not a real dangerous substance, so explaining that I wanted it for educational reasons seemed to be good enough.
I think I undermeasured the amount of luminol the first time around, and this time it may have been slightly overmeasured, which would explain why it was brighter.
ian-albert.com /misc/luminol.php   (1858 words)

  
  Reference.com/Encyclopedia/Luminol
Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes.
Luminol is used by crime scene investigators to locate traces of blood, even if it has been cleaned or removed.
Luminol also fluoresces in the presence of copper or an alloy of copper, horseradish and certain bleaches and as result if a crime scene is thoroughly cleaned with a weak bleach solution (5-6 drops per L), residual bleach will cause the entire crime scene to fluoresce, effectively camouflaging any organic evidence such as blood.
www.reference.com /browse/wiki/Luminol   (609 words)

  
 Demonstrations - Luminol
The luminol is converted by the basic solution into the resonance-stabilized dianion 1, which is oxidized by the hydrogen peroxide into the dicarboxylate ion 2, accompanied by the loss of molecular nitrogen, N
In aqueous solutions, the luminol oxidation is catalyzed by the presence of a metal ion, such as iron(II) or copper(II).
In the demonstration below, the light blue luminol solution and the colorless hydrogen peroxide solution are poured together in a funnel, to which a coil of clear tubing has been attached.
www.angelo.edu /faculty/kboudrea/demos/luminol/luminol.htm   (409 words)

  
 Chem 197 - Luminol Synthesis
An alkaline solution of luminol (1) contains the doubly ionized anion (2) which is oxidized to the triplet excited state (two electrons with the same spin) by a combination of hydrogen peroxide and potassium ferricyanide.
Luminol (1) is prepared by reduction of the nitro derivative (8) formed on thermal dehydration of a mixture of 3-nitrophthalic acid (6) and hydrazine (7).
Dissolve the first crop of moist luminol (dry weight about 40-60 mg) in 2mL of 3M NaOH solution and 18 mL of water; this is stock solution A. Prepare a second stock solution, B, by mixing 4 ml of 3% aqueous potassium ferricyanide, 4 mL of 3% hydrogen peroxide, and 32 mL of water.
cas.bellarmine.edu /chem117a/lab/luminol.htm   (782 words)

  
 It:Luminol - ChemWiki
Luminol exhibits interesting chemistry because it is one of few substances that undergoes reactions which produce light.
Luminol is most commonly used in forensic testing to find traces of blood on crime scenes; the haemoglobin in the blood contains enough iron to catalyse the reaction.
Using luminol on blood samples was first developed by W. Specht in 1937, however it wasn't untill M. Grodsky, in 1951, who developed a solution of sodium bicarbonate and sodium perborate in water that it became useful in an investigative manner.
www.ch.ic.ac.uk /wiki/index.php/It:Luminol   (737 words)

  
 Chemistry - Luminol
Luminol is a chemical used by forensic investigators to detect blood.
Luminol reacts with the iron in haemoglobin, a pigment present in blood.
Luminol also flouresces in the presence of copper or an alloy of copper, certain bleaches and horseradish.
www.chemistrydaily.com /chemistry/Luminol   (175 words)

  
 Luminol: World of Forensic Science
When investigating suspected crime scenes where the visible evidence of crime was removed by the perpetrator, nothing is more useful than luminol, a chemi-luminescent compound, which reacts to red blood cells (hemoglobin) and gives off a blue-greenish light.
Luminol (5–amino–2,3–dihydro–1,4–phthalazine-dione) was accidentally discovered in 1928 by the German chemist H.O. Albrecht, and was first used at a crime scene in 1937 in Germany.
Luminol is highly sensitive to bloodstains or residues, even to old stains, in walls, carpets, upholstery, wooden floors, or painted surfaces.
science.enotes.com /forensic-science/luminol   (138 words)

  
 BLUESTAR Forensic - Chemistry and history
Luminol (3-Aminophthalhydrazide) was synthesized for the first time in 1853.
The first experiments conducted with a view to using luminol as a tool in forensic sciences were conducted in 1937 by Specht, who tested it out on a variety of bases such as the lawn, bricks, or stone soaked in blood.
This subsequently became the formula that is most commonly used by today’s investigators to detect traces of blood at the scene of a crime.
www.bluestar-forensic.com /gb/bluestar-chemistry.php   (441 words)

  
 Achat de matériel et produits scientifiques
Concernant le luminol, le prix est plus proche de 6 euros le gramme que de 350 euros le gramme...
Luminol 98%, les 5 g (référence n°153850050) sont à 49,87 €TTC, soit 11 €TTC le gramme.
Luminol 97% (référence n°123072), les 5 g sont à 54,66 €TTC, soit 10,93 €TTC le gramme.
www.univ-pau.fr /~darrigan/forum/forum_chimie_materiel.php?msg=494   (742 words)

  
 [No title]   (Site not responding. Last check: )
Luminol produces a reaction which is extremely sensitive, capable of detecting bloodstains diluted up to 10,000 times, through the emission of light known as luminescence.
Luminol has proven to be effective on a variety of surfaces: walls, carpets, household and industrial furniture, and even the interior, exterior and trunks of vehicles used to dispose of a victim.
Luminol is noncorrosive but is liable to cause inflammation through immediate, prolonged, or repeated contact with the skin or mucous membranes.
www.clubs.psu.edu /up/sciencelions/demos/LuminolDemo.doc   (1079 words)

  
 The Chemiluminescence of Luminol - Home
Luminol will go grey over time and I found this to be reasonably useless - it should appear a light olive green colour.
The rate of the reaction of luminol is a little more complicated than you will have come across in school but I will attempt to explain it using some interesting concepts.
The luminol reaction could be seen as being made up of two steps; the attack by base and then the subsequent oxidation (the last step is so fast that it will have no effect on the overall rate).
www.chm.bris.ac.uk /webprojects2002/fleming/experimental.htm   (1017 words)

  
 Hemaglow - luminol - Evidence Collection
According to the Merck Index, Luminol is a compound that reacts with the copper, iron peroxides and cyanide.
As luminol reacts with substances not specific to blood, luminol solutions should not be used as a "presumptive test" for blood.
A fresh luminol solution (prepared just before experimentation) composed of.1 gram of luminol, 5.0 grams of sodium carbonate Na2CO3 and 90 milliliters of distilled water combined with an equal amount of 3% hydrogen peroxide (H2O2) was made.
www.securityandsafetysupply.com /news/lp-32.htm   (970 words)

  
 NTP: Luminol (Toxicity)
Luminol, in the absence of metabolic activation, was also reported as negative for the reversion of Escherichia coli to streptomycin independence (Szybalski, 1958).
Luminol, at doses of 250, 500, and 1000 µM, greatly enhanced the frequency of SCE in Chinese hamster V79 cells when treatment occurred during the S-phase in either the first or the second cell cycle in the presence of bromodeoxyuridine (Ikushima, 1990).
Luminol was reported to increase the efficiency of intrachromosomal homologous recombination in Chinese hamster A238 cells (Abramian et al., 1994 abstr.), and to affect the rate of single DNA exchanges and gene conversion (double exchanges) in LMtk
ntp-server.niehs.nih.gov /index.cfm?objectid=6F5FCC82-F1F6-975E-704A80E008D3A806   (495 words)

  
 Oscillating Luminol Reaction
Luminol (3-aminophtalhydrazide) is oxidized by hydrogen peroxide to 3-Amino phtalic acid.
Luminol as a light emitting molecule has been known since 1928 [3] and has since then be the object of extensive mechanistical studies.
Through permutation of the inlet configurations and of the stirring sense, the strength of interactions between these subvolumes and the reactor bulk are altered, which may change the behaviour of the system, as was shown experimentally and computationally for the Belouzhov-Zhabotinsky reaction [4].
www.er.uqam.ca /nobel/k31061/dipl.htm   (870 words)

  
 Discovery Channel :: You're on the Case: Investigator's Toolbox   (Site not responding. Last check: )
Luminol is a chemical that glows greenish-blue when it comes into contact with blood — even traces that are years old.
Luminol is so sensitive, it can detect blood at 1 part per million.
Luminol also can point the way to further evidence or even to how events might have unfolded.
dsc.discovery.com /fansites/onthecase/toolbox/tool_01.html   (356 words)

  
 You   (Site not responding. Last check: )
This is because luminol is a chemical that glows greenish-blue when it comes into contact with blood — even traces that are years old.
This light-emitting property of luminol began to be explored by life science research laboratories some 20 years ago, and now constitutes the basis of “enhanced chemiluminescent (ECL) detection”.
In crime scene investigations, luminol reacts to hemoglobin and is oxidized by heme, resulting in the release of greenish-blue light that can be picked up by various detection instruments.
www.acsu.buffalo.edu /~chunglee/TN-4.htm   (493 words)

  
 Luminol Information
Luminol is used by crime scene investigators to locate occult blood, even if it has been cleaned or removed.
Luminol also fluoresces in the presence of copper or an alloy of copper, certain bleaches and horseradish.
The ratio of luminol solution to luminol activator determines the intensity and the duration of the blue light produced.
www.bookrags.com /wiki/Luminol   (277 words)

  
 eWebProfessionals - How does Luminol help solve crimes? by Deepshikha Khosla   (Site not responding. Last check: )
Luminol is a chemical that glows with a bluish light when it comes in contact with blood.
Luminol is typically used at a crime scene where bloodshed is suspected to have happened but no traces of blood are visible to the naked eye.
Luminol helps to provide clues as to how events may have unfolded or the kind of weapon used.
www.ewebprofessionals.com /articles/Law/How_does_Luminol_help_solve_crimes.htm   (541 words)

  
 NTP: Luminol
The reaction of luminol with oxygen species generated by cells produces an excited intermediate that emits light (chemiluminescence) as it relaxes to a stable state (for reviews see Briheim et al., 1984; Kahl et al., 1987; Radi et al., 1993; Lundqvist and Dahlgren, 1996).
Luminol chemiluminescence occurs in aqueous solution with hydrogen peroxide and a supplemental oxidant such as ferricyanide, hypochlorite, persulfate, or the hydroxyl radical generated from hydrogen peroxide, and a metal derivative such as hemin.
Results from some studies indicate that luminol chemiluminescence in in vitro mammalian systems requires the presence of the enzyme myeloperoxidase (Dahlgren and Stendahl, 1983) and Fe (Klinger et al., 1996) to proceed, and is greatly enhanced in the presence of bicarbonate (Puget and Michelson, 1976; Radi et al., 1993).
ntp-server.niehs.nih.gov /index.cfm?objectid=6F5FCA5F-F1F6-975E-7303709FEC33A9BB   (506 words)

  
 Luminol
Darstellung von Luminol erfolgt über die Nitrierung von Phthalsäure zu 3-Nitrophthalsäure, dessen Umsetzung mit Hydrazin zu 3-Nitrophthalsäurehydrazid und nachfolgender Reduktion mit Natriumdithionit zum Luminol.
Zur Spurensuche werden zwei Lösungen (eine Lösung von Luminol in Natronlauge und eine verdünnte Wasserstoffperoxid-Lösung) hergestellt.
Luminol kann nicht aus 6 Kohlenstoffen bestehen, denn es werden acht benötigt um mit Hilfe der Konstitutionsformel das Molekül zusammen zu bauen.
www.weblexikon.de /Luminol.html   (470 words)

  
 Synthesis of Luminol
Luminol is a chemiluminescent substance that is made by reduction of nitrophthalhydrazide, which is formed upon thermal dehydration of 3-nitrophthalic acid with hydrazine.
  When luminol is allowed to react with hydrogen peroxide and potassium ferricyanide in a basic solution, the emission of a blue-green light is observed.
Dissolve the crude luminol product in 2 mL of 10% sodium hydroxide solution and 38 mL of water – this is “Solution A”.
www2.volstate.edu /msd/CHE/242/Labs/luminol.html   (494 words)

  
 SIRCHIE Finger Print Laboratories, Inc.   (Site not responding. Last check: )
Description: WITH SPRAY HEAD Luminol is a chemiluminescent substance that can be used as a presumptive test for the presence of blood.
Making use of the peroxidaselike activity of the heme portion of hemoglobin, Luminol produces a bluish-white light that can be viewed in total darkness.
Invisible blood stains react with the Luminol reagent by luminescing— darkness is essential.
www.sirchie.com /detail.asp?product_id=LUMINOL4   (183 words)

  
 L37. Non-Linear Phenomenon in Magnetic Field Sensible Recombination of Radicals in Luminol Chemiluminescent Reaction in ...
The chemiluminescent luminol oxidation by Fe in aqueous solution is chosen as a test system to demonstrate kinetic phenomena originating from non-linear dependency of the rate constant of recombination of luminol radicals on the acting factor (magnetic field).
For luminol chemiluminescence oscillations, the same basic harmonics were also observed, but their amplitudes were rapidly decreasing with the increase of their frequencies.
The lifetime of luminol radicals was derived from this distortion and it was in a good agreement with reported elsewhere.
www.cstl.nist.gov /div838/kinet_conf/agenda/l_session/l37.html   (691 words)

  
 Demonstration 24
Luminol in flasks (Chemiluminescence of luminol in dimethylsulphoxide).
C does not prevent the normal reaction of light generation from taking place.  The light, however, does go out faster because all the oxygen is used rapidly; because of the presence of caustic, this then becomes a way to produce nearly pure nitrogen.  It was also noted that lowering the temperature to 13
            Safety.  The toxicity of luminol is not known exactly.  Sensibilisation due to inhalation of luminol dust or to skin contact is possible.  Contact with solid KOH can cause severe skin damage and must be avoided.
www.chem.leeds.ac.uk /delights/texts/Demonstration_24.htm   (329 words)

  
 SuperLuminol Superoxide/Reactive Oxygen Species Kit- WPI   (Site not responding. Last check: )
Assay Principle: Luminol reacts with superoxide and other reactive oxygen species resulting in a luminophore that has an emission peak at ~425 nm.
Indeed, luminol assay is one of the most important methods in the analysis of metabolic activities of phagocytes and is widely used in biomedical research.
WPI Enhancer: A number of compounds are known to increase the intensity of luminescent emission from luminol.
www.wpiinc.com /products/biosensing/superluminol   (396 words)

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