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Topic: Lysozyme


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In the News (Wed 21 Aug 19)

  
  Lysozyme - Wikipedia, the free encyclopedia
Lysozyme is an enzyme (EC 3.2.1.17), commonly referred to as the "body's own antibiotic" since it kills bacteria.
Lysozyme serves as an unspecific innate opsonin by binding to the bacterial surface to reduce the negative charge and facilitate phagocytosis of this bacterium before opsonins from the acquired immune systems enter the scene.
Lysozyme levels in the blood are often increased in sarcoidosis.
en.wikipedia.org /wiki/Lysozyme   (316 words)

  
 American Journal of Respiratory and Critical Care Medicine: Lysozyme in Pulmonary Host Defense: New Tricks for an Old ...
Lysozyme damages the cell walls of bacteria and fungi by hydrolyzing the [beta]1-4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine, which are structural components of bacterial peptidoglycan and fungal chitin, respectively.
Lysozyme M is the major form expressed in murine epithelial cells and leukocytes, and lysozyme P is normally limited to the Paneth cells of the small intestine (8, 9).
Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglyean.
www.findarticles.com /p/articles/mi_qa4085/is_200402/ai_n9367726   (1212 words)

  
 WINE BUSINESS Printer version
Lysozyme is a protein derived from egg whites, with a lytic activity towards Gram-positive bacteria.
Until recently, the use of lysozyme was mostly recommended to delay or block malolactic fermentation, prevent unwanted bacterial growth during storage or ageing, and inhibit further bacterial spoilage in the case of stuck or sluggish fermentation.
The prophylactic use of lysozyme is especially recommended in wines of high pHs; wines with a history of stuck or sluggish fermentation or a history of high levels of volatile acidity and off-flavors, as well as wines or wineries that have frequent contamination problems with Lactobacilli or Pediococci", says Petersen.
winebusiness.com /html/PrinterVersion.cfm?dataId=20409   (1562 words)

  
 BYO - Lysozyme
Lysozyme is found abundantly in nature and is produced by bacteria, fungi, plants, birds, and mammals.
Although lysozyme is found in high concentrations in human tears, commercial lysozyme is made from hen egg whites.
Lysozyme is effective against Gram positive bacteria (all lactic acid bacteria) due to its ability to specifically break down the cell wall structure of those microorganisms.
www.byo.com /feature/1162.html   (2286 words)

  
 Lysozyme, Serum
Lysozyme, an hydrolytic enzyme - a bacteriolytic glycosidase, when present in large amounts may appear as a far cathodal migrating ("cationic") band on serum or urine protein electrophoresis.
Serum lysozyme has been shown to be elevated in a number of conditions, including tuberculosis and sarcoidosis as well as leukemia.
Utilizing a turbidimetric method for measurement of serum lysozyme activity, there was evidence that such an assay was useful in differentiating infection from rejection in transplant recipients.
www.labcorp.com /datasets/labcorp/html/chapter/mono/sc015050.htm   (611 words)

  
 Chemistry Research   (Site not responding. Last check: 2007-11-02)
Lysozyme comes from many plant and animal sources, but is especially plentiful in hen egg whites.
Each egg white had a different mass, and since the amount of lysozyme in each egg white is proportional to it's mass, it is necessary to compare the amounts of lysozyme extracted in terms of percentages.
Work with lysozyme is constantly being completed because of how commercially useful it is. One current study is interested in enabling lysozyme to work on gram-negative bacteria as well as those that are gram-positive.
www.heidelberg.edu /depts/chm/lysozyme1.html   (2092 words)

  
 Title of Invention: Transformed yeasts for producin g lysozyme, plasmids used for this transformation, method of ...
Lysozyme production capacity is therefore partly ruled by the egg white market, and this poses a problem more difficult to solve in that technical difficulties, and even in some countries legal restraints, limit the use of treated egg white in standard foodstuffs.
Their lysozyme activity was tested by determining the decrease in optical density of a suspension of E. coli cells by the method of Mc Macken et al.
Lysozyme activity in the supernatant and the lysate from both cultures was determined by their ability to lyse M.
www.nal.usda.gov /bic/Biotech_Patents/1994patents/05336609.html   (5134 words)

  
 Influenza Virus Inhibits Lysozyme Secretion by Sputum Neutrophils in Subjects with Chronic Bronchial Sepsis -- PANG et ...
Lower respiratory tract lactoferrin and lysozyme arise primarily in the airways and are associated with chronic bronchitis.
Lysozyme mediated aggregation and lysis of the periodontal microorganism Capnocytophagia gingivalis 2010.
In vitro release of lysozymes from monocytes and granulocytes.
ajrccm.atsjournals.org /cgi/content/full/161/3/718   (2892 words)

  
 Isolation and Analysis of Lysozyme from Egg Whites   (Site not responding. Last check: 2007-11-02)
Lysozyme is a globular protein containing 129 amino acids that was discovered in 1922 by Alexander Flemming by accident (5).
Lysozyme was first introduced to the author the summer of 1998 in a biochemical laboratory at Bowling Green State University (BGSU).
At first all lysozyme molecules are available to bind to substrate; but as the enzyme (lysozyme) activities begin to "fill-up" with substrate (Ml), the observed effect is a leveling off of enzyme activity.
www.heidelberg.edu /depts/chm/lypozyme.html   (2811 words)

  
 Title of Invention: Use of lysozyme gene structures in plants to increase resistance
Lysozyme genes are understood as meaning any nucleic acid (DNA) which, after having been transcribed into RNA and translated into protein, effects (in a suitable environment) the formation of an enzyme which has the known properties of lysozymes.
Preferred lysozyme gene structures which can be used according to the invention consist of, or contain, chimeric gene fusions which, in addition to the lysozyme gene(s), contain the TR promoter and the signal peptide sequence of barley alpha-amylase, as they occur in plasmid pSR 2-4.
The lysozyme genes can be completely present in the gene structures, that is to say with their natural regulatory parts (in particular promoters) or only in the form of the structural genes which encode the protein lysozyme.
www.nal.usda.gov /bic/Biotech_Patents/1994patents/05349122.html   (6156 words)

  
 Lactoferrin and Lysozyme Supplements - DrGreene.com   (Site not responding. Last check: 2007-11-02)
Human lactoferrin and human lysozyme are both proteins that are important ingredients in breast milk.
Lysozyme was the first antibacterial substance discovered by Alexander Fleming, who later went on to discover penicillin.
Lysozymes (and lactoferrins) may be divided into human lysozyme (and lactoferrin) and that from other sources in the plant and animal world.
www.drgreene.com /21_1407.html   (561 words)

  
 Lysozyme   (Site not responding. Last check: 2007-11-02)
This illustration represents a model of the 3-dimensional structure for the protein lysozyme.
Lysozyme is a an enzyme that destroys bacterial cell walls by hydrolyzing the polysaccharide component of the cell wall.
Lysozyme can be found in the mucosal membranes that line the human nasal cavity and tear ducts.
crystal.uah.edu /~carter/protein/lysozym.htm   (128 words)

  
 Lysozyme Kinetics
In your report, enter the final lysozyme dilution factor (example: 1:4, which means that 1 mL of stock lysozyme was diluted to a final volume of 4 mL) and the lysozyme assay concentration in your last range-finding assay (mg lysozyme per mL in the assay tube) in the Table of Results (see Section V, Report).
Add the lysozyme to the reaction tube while it is still in the water bath, and then quickly remove the reaction tube, vortex it, and put it into the spectrophotometer.
by the concentration of lysozyme in your assay tubes.
www.usm.maine.edu /~rhodes/BiochemLab/Text/HdtKinetics/HdtKinetics.html   (3923 words)

  
 Lysozyme: Genetic Music   (Site not responding. Last check: 2007-11-02)
Lysozyme has been set separately by Clark and by Dunn and the difference between the pieces shows the rich musical possibilities inherent in protein sequences.
Clark's Lysozyme C is a fairly placid read-through of the sequences from four different species; the voices, when playing together, are always simultaneous and singing in the same key to demonstrate their similarities.
The piece is introduced by a slow flute voice that continues as a descant through the entire piece as a reminder of the long history of this protein.
www.whozoo.org /mac/Music/lysozyme.htm   (339 words)

  
 The Effect of Lysozyme on Elastase-Mediated Injury -- Cantor et al. 227 (2): 108 -- Experimental Biology and Medicine
Although lysozyme did not significantly affect elastic fiber breakdown by pancreatic elastase (bar 1 vs 2), it did interfere with the protective effect of HA (bars 3 and 4 vs 5).
lysozyme was mainly associated with alveolar macrophages (1,14).
The characteristics of lysozyme and carbenicillin action on the clinico-immunological status of patients with chronic bronchitis.
www.ebmonline.org /cgi/content/full/227/2/108   (3241 words)

  
 ENZYME KINETICS CONTINUED:
For T4 lysozyme no group corresponding to Glu-35 is found, suggesting that solvent water serves that purpose in this particular enzyme.
The pH dependence of lysozyme catalysis reveals that the reaction is dependent on an acid with pK ~6 and a base of pK ~ 4 in the free enzyme (from plots of k
Site-directed mutagenesis in which Asp52 and Glu35 were converted to the corresponding Asn and Gln demonstrated the critical requirement for Glu35 (0% activity) and lesser essentiality of Asp52 (5% initial velocity, 0.5% SS, against cell wall substrates) (good reference: Malcolm et al., PNAS 86 133-137, 1989).
www.chemistry.ucsc.edu /~fink/231/lecture12.htm   (791 words)

  
 Worthington Lysozyme
Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.
Lysozyme is of widespread distribution in animals and plants.
Spinal-Fluid Lysozyme in the Diagnosis of Central-Nervous-System Tumours, Lancet 2, 756, 1974
www.worthington-biochem.com /LY   (4647 words)

  
 Posttranscriptional Lipopolysaccharide Regulation of the Lysozyme Gene at Processing of the Primary Transcript in ...
The blots were hybridized to lysozyme cDNA, intron 1, and intron 2 and were subsequently stripped and rehybridized to GAPDH cDNA.
the lysozyme mRNA decay and a consequence of the poly(A) tail
Structure of the lysozyme gene and expression in the oviduct and macrophages.
www.jimmunol.org /cgi/content/full/160/10/4970   (6263 words)

  
 Lysozyme antibody - Lysosome Marker (ab2408) datasheet   (Site not responding. Last check: 2007-11-02)
Lysozyme isolated from urine of patients with monocytic leukemia.
If Lysozyme antibody - Lysosome Marker (ab2408) does not perform as described on this datasheet, notify us within 90 days of delivery using this form, so that we can help you or offer you a replacement or a refund.
Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls.
www.abcam.com /?datasheet=2408   (362 words)

  
 Self perceived work related stress and the relation with salivary IgA and lysozyme among emergency department nurses -- ...
Lysozyme determination in parotid saliva from patients with Sjogren’s syndrome.
Salivary lysozyme: a noninvasive marker for the study of the effects of stress on natural immunity.
Lysozyme and IgA concentration in serum and saliva from psoriatic patients.
oem.bmjjournals.com /cgi/content/full/59/12/836   (3516 words)

  
 Inactivation of Gram-Negative Bacteria by Lysozyme, Denatured Lysozyme, and Lysozyme-Derived Peptides under High ...
The experiments with the denatured lysozymes under high pressure (Tables 2 and 3) lead to two observations.
pressure-promoted uptake of lysozyme is not a universal phenomenon
The chemistry of lysozyme and its use as a food preservative and a pharmaceutical.
aem.asm.org /cgi/content/full/67/1/339   (3528 words)

  
 Lysozyme, the Foreskin, and HIV Protection
Lysozyme is an enzyme with anti-bacterial action that is found in body fluids.
Fleiss and others report that the increased friction and more vigorous and prolonged thrusting required to achieve orgasm with a circumcised penis may be more likely to cause "breaks, tears, microfissures, abrasions, and lacerations through which HIV in semen can enter the receiving partner's bloodstream."
Lysozyme and RNases as anti-HIV components in beta-core preparations of human chorionic gonadotropin.
www.foreskin.org /lysozyme.htm   (463 words)

  
 lysozyme --¬† Encyclop√¶dia Britannica
Discovered in 1921 by Sir Alexander Fleming, lysozyme catalyzes the breakdown of certain carbohydrates found in the cell walls of certain bacteria (e.g., cocci).
It thus functions, in the case of lacrimal fluid, to protect the cornea of the eye from infection.
Lysozyme is an enzyme that hydrolyzes the carbohydrates found in the...
www.britannica.com /eb/article-9049558?tocId=9049558   (574 words)

  
 Lysozyme   (Site not responding. Last check: 2007-11-02)
Lysozyme is an enzyme found in egg white, tears, and other secretions.
The primary structure of egg white lysozyme - shown here - is a single polypeptide chain of 129 amino acids.
Rather the chain must fold to allow these cysteines to be close to each other.
users.rcn.com /jkimball.ma.ultranet/BiologyPages/L/Lysozyme.html   (99 words)

  
 Mouse Lysozyme M Is Important in Pulmonary Host Defense against Klebsiella pneumoniae Infection -- Markart et al. 169 ...
Mouse Lysozyme M Is Important in Pulmonary Host Defense against Klebsiella pneumoniae Infection -- Markart et al.
of the rat lysozyme cDNA to the distal respiratory epithelium
Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycan.
ajrccm.atsjournals.org /cgi/content/full/169/4/454   (2834 words)

  
 Frederick Dahlquist   (Site not responding. Last check: 2007-11-02)
For instance, one problem is how the dynamics of a protein are related to its structure and thermodynamic stability.
Mulder, F.A., B. Hon, A. Mittermaier, F.W. Dahlquist, and L.E. Kay (2002) Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme.
Lu, J. and F.W. Dahlquist (1992) Detection and characterization of an early folding intermediate of T4 lysozyme using pulsed hydrogen exchange and 2-D NMR.
morel.uoregon.edu /home97/dahlquist.html   (1672 words)

  
 Lysozyme antibodies from RDI Divison of Fitzgerald Industries Intl
Lysozyme antibodies from RDI Divison of Fitzgerald Industries Intl
Also available: human lysozyme (pure) cat#RDI-SCP113-1 $250.00/1mg $875.00/10mg)
Clinical Application/ Specificity LYZ-Z blocks the catalytic activity of human lysozyme.
www.researchd.com /miscabs/pro61083.htm   (405 words)

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