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Topic: MALDI-TOF

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bdal.com ultraflex II and ultraflex II TOF-TOF Bruker Daltonics ultraflex II and ultraflex II TOF/TOF The "TOF only" configuration is field upgradeable to full MALDI-TOF/TOF capability. Low abundant proteins can be identified and characterized at great detail using MALDI-TOF/ TOF. The ultraflex II uses our patented gridless ion source and reflectron design, providing unsurpassed sensitivity in the standard MALDI-TOF MS mode. www.bdal.com /modux3/modux3.php?pid=105,000,000,01,01,006,009,0&rid=105,000,000,01,01,006,001,0&width=1152&height=864   (1058 words)

Advances In MALDI-TOF Tools for Mass Spectrometry - Biocompare Technology Spotlight In this orthogonal geometry, the MALDI source is decoupled from the TOF thereby eliminating any discrepancies associated with ionization and/or the sample target. Processing for MALDI TOF Mass Spectrometry has now been integrated with a new approach in high throughput proteome analysis. Xcise™, the "fully integrated" automated gel processor for 1D/2D gel-based proteomics, combines the functions of gel acquisition imaging, protein spot cutting and digestion, peptide extraction and deposition onto a MALDI target plate in a single integrated benchtop instrument. www.biocompare.com /spotlight.asp?id=130   (780 words)

Bruker Daltonics Autoflex II Maldi-Tof/Tof Mass Spectrometers Bruker Daltonics’ new autoflex TOF/TOF makes use of gridless ion source and reflectron design, providing the unsurpassed sensitivity in standard MALDI-TOF MS mode. The autoflex TOF/TOF takes advantage of our leadership in high throughput MALDI-TOF design for industrial biology. With our decades of experience supplying the most successful autoflex TOF and ultraflex TOF/TOF, state-of-the art analytical performance has been engineered into the autoflex TOF/TOF from the outset. www.labcentraal.com /webinventory/Bruker_Autoflex_II_Tof_Tof_Mass_Spectrometer.htm   (1100 words)

MALDI MS For any MALDI experiment employing TOF mass spectrometers with continuous ion extraction, metastable ion decay occurring in the ion source during ion acceleration produces incoherent ion noise in the resulting mass spectrum. In a linear TOF mass spectrometer, metastable fragmentation which occurs post-source (i.e., in the field free region) is not apparent since precursor and metastable decay ions move with the same velocity (provided there are no post-source electrostatic fields) and arrive simultaneously at the detector. The samples were inserted into the TOF mass spectrometer and the masses of the truncated (i.e., ladders) peptides were determined. www.abrf.org /ABRFNews/1997/June1997/jun97lennon.html   (3622 words)

Peptide sequencing Macromolecular Resources - automated analysis by Edman degradation; standard protein identification consists of MALDI-TOF mass spectrometry analysis of peptides resulting from the enzymatic digestion of the target protein; MS/MS spectra are collected using MALDI-TOF/TOF for de novo sequencing of peptides Harvard University Microchemistry and Proteomics Analysis Facility - Edman and C-term sequencing and peptide/protein ID using microcapillary LC-MS/MS on 3D and linear ion traps/ MALDI-TOF and hybrid quadrupole-TOF mass spectrometers Commonwealth Biotechnologies - N-terminal protein sequence analysis performed by automated Edman degradation or C-terminus sequence analysis by carboxypeptidase Y digestion. www.peptideresource.com /sequencing.html   (236 words)

Mass spectrometry glossary Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometry: With MALDI-TOF (matrix- assisted laser desorption- ionization time- of- flight) mass spectrometry, a laser beam passes through the substances to be analyzed, and the laser causes these elements to vaporize and their molecules to fly upward into a tube. The [Sequenom] MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. The MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. www.genomicglossaries.com /content/mass_spectrometry.asp   (236 words)

Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF -- Smylie et al. 14 (1): 134 -- Genome Research Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF -- Smylie et al. Shchepinov, M.S., Denissenko, M.F., Smylie, K.J., Wörl, R.J., Leppin, A.L., Cantor, C.R., and Rodi, C.P. Matrix-induced fragmentation of P3'-N5' phosphoramidate-containing DNA: High-throughput MALDI-TOF analysis of genomic sequence polymorphisms. www.genome.org /cgi/content/full/14/1/134   (236 words)

Burle Electro-Optics Inc.'s Time-of-Flight Detector - Photonics.com Featured Products    Whether you are working with MALDI-TOF, ESI-TOF, GC-TOF, TOF-TOF or custom TOF mass spectrometry, the MCP detector is a key factor in the mass resolution of your instrument. STURBRIDGE, Mass., Jan. 13 -- Burle Electro-Optics Inc.'s 25 mm collection diameter time-of-flight (TOF) detector uses 5-micron small-pore MCP technology to improve mass resolution while gaining subnanosecond pulse widths for previously unobtainable levels of mass resolution, dynamic range and detection sensitivity. Plus, the detector's flat input surface provides uniform ion conversion and the higher aspect ratio of the MCP produces gains in excess of 107. www.photonics.com /XQ/ASP/url.readprod/prodid.481/QX/readprod.htm   (236 words)

Mapping Cu(II) Binding Sites in Prion Proteins by Diethyl Pyrocarbonate Modification and Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometric Footprinting -- Qin et al. 277 (3): 1981 -- Journal of Biological Chemistry Mapping Cu(II) Binding Sites in Prion Proteins by Diethyl Pyrocarbonate Modification and Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometric Footprinting -- Qin et al. Articles by Qin, K. Articles by Westaway, D. Mapping Cu(II) Binding Sites in Prion Proteins by Diethyl Pyrocarbonate Modification and Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometric Footprinting The abbreviations used are: PrP, prion protein; MALDI, matrix-assisted laser desorption ionization; MS, mass spectrometry; DEPC, diethyl pyrocarbonate; TOF, time of flight; PSD, post-source decay; ESI, electrospray ionization; NEMO, N -ethylmorpholine. www.jbc.org /cgi/content/full/277/3/1981   (236 words)

Experimental techniques: MALDI-TOF Mass spectrometry Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) is a relatively novel technique in which a co-precipitate of an UV-light absorbing matrix and a biomolecule is irradiated by a nanosecond laser pulse. A fully equiped MALDI-TOF MS instrument, a Bruker BIFLEX, is present in our department. Radboud university > Faculty of Science > Biology > Microbiology > Techniques > MALDI-TOF www.micrbiol.sci.kun.nl /tech/malditof.html   (315 words)

UNC CH Proteomics Core Facility ABI 4700 Proteomics Analyzer (TOF/TOF) This instrument is a MALDI-MS and MS/MS mass spectrometer with a mass accuracy of 5 to 10 ppm, and a resolution of 25,000. The accurate molecular weight and partial sequence information from MALDI TOF/TOF analysis of even a single peptide is often sufficient to identify a protein or a family of homologous proteins containing that peptide. Borchers and Parker have worked jointly in the area of proteomics research since 1997, have 15 joint publications, and are responsible for the design and coordination of the entire process of the mass spectrometric protein identification including quality control, method validation, and data quality assurance. proteomics.unc.edu /corestmt.shtml   (5630 words)

APSGFLGMRamide is a unique tachykinin-related peptide in crustaceans -- Yasuda-Kamatani and Yasuda 271 (8): 1546 -- FEBS Journal Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Kubo, T. and Nakajima, T. (2003) Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen, and drone heads. Yasuda-Kamatani, Y. and Yasuda, A. (2000) Identification of orcokinin gene-related peptides in the brain of the crayfish Procambarus clarkii by the combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectrometries and molecular cloning. Yasuda, A., Yasuda-Kamatani, Y., Nozaki, M. and Nakajima, T. (2004) Identification of GYRKPPFNGSIFamide (crustacean-SIFamide) in the crayfish Procambarus clarkii by topological mass spectrometry analysis. content.febsjournal.org /cgi/content/full/271/8/1546   (4714 words)

Biopharmaceutical Mass Spectrometry glossary The MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. The [Sequenom] MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. In a TOF analyzer, ions are separated by differences in their velocities as they move in a straight path toward a collector in order of increasing mass- to- charge ratio. www.genomicglossaries.com /content/mass_spectrometry.asp   (3198 words)

Introduction to Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (MALDI TOF MS) A great advantage of MALDI TOF MS is that the process of soft-ionisation causes little or no fragmentation of analytes, allowing the molecular ions of analytes to be identified, even within mixtures. MALDI was developed for the ionisation of relatively large polypeptides and proteins but its application has widened to incorporate glycoproteins, oligonucleotides and complex carbohydrates. The excitation of the matrix by a high intensity laser pulse of short duration, the absorbed energy causing desorption (vaporisation) and ionisation of the analyte in a very dense MALDI plume. www.kcl.ac.uk /ms-facility/maldi.html   (1231 words)

Applied Bio, Invitrogen forge proteomics alliance Applied Biosystems will also provide software support for SILAC on its TOF/TOF family of products, including the newly launched 4800 MALDI TOF/TOF Analyzer, and plans to extend software support for the SILAC labelling technology to other Applied Bio/MDS SCIEX protein mass spectrometry systems. "The alliance with Invitrogen brings together the capabilities of two leaders in their respective fields to deliver improved solutions for proteomics and enable biologists to further elucidate cellular pathways that are implicated in disease. In proteomics, these reagents tend to be used in experiments such as time course studies or expression profiling studies for putative biomarkers. www.drugresearcher.com /news/news-ng.asp?n=61231-applied-bio-invitrogen   (471 words)

Lib #: WA20256 - Waters LC-MALDIprep Sample Collection Module: Improved Peptide Mass Fingerprinting from Continuous Sample Tracks Traditionally, PMF is performed by MALDI-TOF/MS analysis of non-separated digests deposited onto sample wells. Peptide mass fingerprinting (PMF) is a useful method for rapid identification of proteins using MALDI mass spectrometry. The MALDI/MS instrument is then used to scan along this track to generate a mass chromatogram of the separation for use in PMF. www2.waters.com /watprod.nsf/newdocs/WA20256   (249 words)

Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF -- Smylie et al. 14 (1): 134 -- Genome Research Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF -- Smylie et al. Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF The challenge in the postgenome era is to measure sequence variations www.genome.org /cgi/content/abstract/14/1/134   (298 words)

Nanoliter The patent has direct application to MALDI TOF and ESI/MS for detection of biomarkers (i.e., cancer and disease markers), and in proteomics, genomics, diagnostics and forensics. One reason for this interest is that "protein patterns" identified by MALDI TOF have been shown to diagnose ovarian, prostrate, lung, brain, colon, pancreatic and other cancers and other diseases (Alzheimer's). Nanoliter is a group of analytical chemists and others simply developing and applying technology to interesting macro and microfluidic and other areas as we have successfully done over thirty years. www.nanoliter.com /aboutus.htm   (298 words)

Biopolymer Services A MALDI-TOF mass spectrometer measures the masses of individual inoized molecules as a function of their time of flight down a tube of known length. The biopolymers laboratory mass spectrometric analysis uses Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) instrumentation. Mass Spectrometry is a powerful analytical technique for the accurate mass measurement of synthetic peptides, oligonucleotides, and proteins. dahlweb.med.harvard.edu /biopserv.shtml   (1928 words)

ARS Publication request: Identification of Iron-Regulated Outer Membrane Proteins of Mannheimia Haemolytica by Comparative 2-D Electrophoresis, Western Blotting, and Maldi-Tof (Poster Present. - 102nd Meet. of the Am. Soc. for Microbiology) Publication request: Identification of Iron-Regulated Outer Membrane Proteins of Mannheimia Haemolytica by Comparative 2-D Electrophoresis, Western Blotting, and Maldi-Tof (Poster Present. Title: Identification of Iron-Regulated Outer Membrane Proteins of Mannheimia Haemolytica by Comparative 2-D Electrophoresis, Western Blotting, and Maldi-Tof (Poster Present. The proteins may be further utilized to study their role in MH colonization of the nasopharynx. www.ars.usda.gov /research/publications/publications.htm?SEQ_NO_115=128451&pf=1   (349 words)

Genencor: Publications Impact of prefractionation on two-dimensional gel electrophoresis and protein identification by MALDI-TOF mass spectrometry. Impact of deglycosylation methods on two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry for proteomics Naik, R.R., Jones S. E., Murray, C. J., McAuliffe, J. C., Vaia R. A., Stone, M. Characterization of Secretory Genes ypt1/yptA and nsf1/nsfA from Two Filamentous Fungi: Induction of Secretory Pathway Genes of Trichoderma reesei under Secretion Stress Conditions www.genencor.com /wt/gcor/publications   (2865 words)

Protein and Peptide Letters, Vol. 9, No. 1, 2002 Ca(II) - g- Thionin Complex: Interaction Studies by Differential Pulse Voltammetry and Maldi-Tof/Ms Clarissa Silva Pires de Castro, Jurandir Rodrigues DeSouza and Carlos Bloch J ْ nior In this work we investigated the formation of a complex between g -thionin SI a1 and Ca using differential pulse voltammetry and MALDI TOF/MS. A stoichiometry rate of one calcium ion per one SI a 1 molecule was obtained. www.bentham.org /ppl/ppl9-1.htm   (2865 words)

IngentaConnect Peptide-Mass Profiles of Polyvinylidene Difluoride-Bound Proteins... Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), in conjunction with enzymatic digestion of proteins and molecular weight search of peptide-mass database is a powerful technique for peptide/protein identification. This study demonstrates that by using a modified matrix solution, peptide-mass fingerprinting of PVDF-bound proteins by MALDI-TOF can be obtained in the presence of nonionic detergents such as hydrogenated Triton X-100 (RTX-100), octylglucopyranoside, and Tween 20. In our laboratory, enzymatic digestion and extraction of peptides from polyvinylidene difluoride (PVDF)-bound proteins is performed in the presence of nonionic detergents. api.ingentaconnect.com /content/ap/ab/1996/00000233/00000001/art00012   (208 words)

Mass spectrometry glossary The MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. The [Sequenom] MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. www.genomicglossaries.com /content/mass_spectrometry.asp   (208 words)

Mass spectrometry glossary The MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. The [Sequenom] MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. www.genomicglossaries.com /content/mass_spectrometry.asp   (208 words)

Mass spectrometry glossary The MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. The [Sequenom] MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. A critical step in the mass spectrometric characterization of biological materials (analytes) is the process of desorbing the analyte from a surface or matrix into the vacuum of the mass spectrometer. www.genomicglossaries.com /content/mass_spectrometry.asp   (208 words)

Sonderforschungsbereich 539 - Teilprojekt C.1 - Publikationen Bauer M, Humeny A, Schweikert A, Berkholz A, Lausen B, Becker CM and Wildt L. Association of the polymorphic T-allele in the coding G1978T polymorphism (Leu660Val) in the human progesterone receptor gene (hPR) with recurrent spontaneous abortions: Genotyping by MALDI-TOF mass spectrometry. Bauer M, Humeny A, Berkholz A, Lausen B, Becker CM and Wildt L. Analysis of the coding polymorphism G1978T (Leu660Val) in the human progesterone receptor (hPR) gene in patients with endometriosis: Genotyping by MALDI-TOF mass spectrometry. Lausen B. Bioinformatics and classification: The analysis of genome expression data. www.imbe.med.uni-erlangen.de /~teilpc1/publikationen.html   (1131 words)

Mass spectrometry glossary The MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. The [Sequenom] MALDI-TOF mass spectrometer measures the exact mass of DNA fragments and is capable of accurately predicting the sequence of a DNA fragment based on the molecules mass. A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. www.genomicglossaries.com /content/mass_spectrometry.asp   (1131 words)

MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) -TIME OF FLIGHT (TOF) SPECTROMETRY - A NEW TOOL TO STUDY COMPLEX EQUILIBRIA Matrix Assisted Laser Desorption Ionization (MALDI) -Time of Flight (TOF) Spectrometry - A new tool to study complex equilibria ? It will be shown for the first time here that MALDI-TOF can also be applied to determine molecular weight of species formed by interaction of the reacting components (metal ions - ligand) and thus to study complex formation. The sample is mixed with a matrix which isolates sample molecules, absorbs energy from the pulsed laser beam and provides photoexcited sites for ionization of analyte molecules in ion/molecule collisions. fc.udg.es /~analitic/simec98/Havel.html   (234 words)

Monitoring of peptide acylation inside degrading PLGA microspheres by capillary electrophoresis and MALDI-TOF mass spectrometry In the degrading PLGA microspheres of sCT and hPTH"1"-"3"4, the acylation products were observed and determined to be adducts with glycolic acid units from degradable PLGA polymer by MALDI-TOF MS. Capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used for determining and monitoring peptide acylation and quantitating acylation products in the degrading PLGA microspheres. The purpose of this research was to assess the acylation reactions of peptides, salmon calcitonin (sCT), human parathyroid hormone 1-34 (hPTH"1"-"3"4) and leuprolide, in poly(lactic-co-glycolic acid) (PLGA) microspheres. www.aapspharmaceutica.com /search/view.asp?ID=46828   (291 words)

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