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Topic: MacConkeys Agar

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	Exercise 2: Isolation and Pure Culture Technique (Site not responding. Last check: 2007-10-18)
		MacConkeys agar (MAC), for example, supports preferentially the growth of Gram negative organisms and inhibits the growth of most Gram positive organisms due to the presence of crystal violet and bile salts.
		Inoculate each pure culture and the mixed culture to a separate agar plate by flaming the inoculating loop in the bunsen burner and then transferring a loopful of bacterial inoculum from the broth culture to the plate.
		Subculture one isolated colony to a second agar plate and streak for isolation as indicated above.
	dentistry.ouhsc.edu /intranet-Web/Courses/DMI_8351/Exercise_2.html (731 words)

	Media Facility Order Form - Bacteria
		28 "M9 Minimal Salts Agar Ampicillin-100 Plates" sm plate
		26 "M9 Minimal Salts Agar Plates" sm plate
		30 "M9 Minimans Salts Agar Ampicillin-100; Calcium Chloride Plates" sm plate
	www.ccc.columbia.edu /media/web-pages/bacteria.html (1903 words)

	MacConkey Agar (Site not responding. Last check: 2007-10-18)
		MacConkey agar is a differential plating medium recommended for use in the isolation and differentiation of lactose-fermenting organisms from nonfermenting Gram-negative enteric bacteria.
		The differential action of MacConkey Agar is based on fermentation of lactose.
		Inoculate the specimen directly onto the surface of a MacConkey agar medium plate and streak for isolation.
	dentistry.ouhsc.edu /intranet-Web/Courses/DMI_8351/MacConkey.html (264 words)

	ARS \| Publication request: Air Quality and Its Microbial Component Is Important to Rural Areas
		All bacteria were collected for a 5 min interval on brain heart infusion agar (BHI) with antifungal inhibitors.
		Fungi were collected for 15 min on malt agar with antibiotic inhibitors.
		Passive gravity BHI and MacConkeys plus Brilliant Green plates mounted vertically at 1 m height were replicated 6 times upwind and downwind, and exposed for 3 and 15 min respectively.
	www.ars.usda.gov /research/publications/Publications.htm?seq_no_115=132965&pf=1 (370 words)

	Patent 6,406,688
		The organisms are tested for antibiotic sensitivity by the disc-agar diffusion technique and found to be sensitive to gentamicin, ampicillin, cephalothin, chloramphenicol, kanamycin, tetracycline, trimethoprin/sulfamethoxazole and resistant to penicillin G, erythromycin, and vancomycin.
		The organism is animal passed in mice and subsequently recovered and plated onto MacConkey's agar.
		All inoculum sizes are based on viable counts determined by scoring colony forming units on MacConkeys agar.
	www.pharmcast.com /Patents/Yr2002/June2002/061802/6406688_Sepsis061802.htm (1290 words)

	[No title] (Site not responding. Last check: 2007-10-18)
		Growth on Blood agar and EMB agar, Large mucoid colonies that ferment lactose, Oxidase negative, Respiratory infections.
		Growth on Blood agar and MacConkeys agar with small round colonies that ferment lactose, Oxidase negative.
		Growth on Blood agar and EMB agar, non-lactose fermenting colonies, Oxidase positive.
	bioweb.wku.edu /faculty/Clauson/208exam3review.html (181 words)

	Methods
		Flame an inoculating loop and obtain a sample of the reddish purple bacterial growth from the bread.
		Using streak plate techniques, streak the sample on MacConkeys agar plates containing 1% lactose.
		If red-pigmented colonies are present, streak DTC media plates, an enrichment media, with individual colonies obtained from MacConkeys agar plates.
	www.life.umd.edu /cbmg/faculty/asmith/200HONORS/WEBPAGE/spring2003/SangeethaRamya/Methods.html (200 words)

	Udder Health Systems Inc.
		Individual milk samples are plated on a minimum of a quarter of a Blood Agar plate, using a minimum of 0.05 ml of milk, unless an enhancement technique is used (freeze thaw, preincubation, etc.).
		Beddings are plated onto three medias: MacConkeys, Modified Edwards and Inulin.
		Two dilutions are made for each media: a 1 to 10,000 and a 1 to 100.000.
	www.udderhealth.com /labop.htm (2722 words)

	weekofApril1
		If going into solid, cool the loop or needle first by sticking it into the agar, and then touching the colony.
		Bacteria are typically grown on some kind of all purpose medium such as tryptic soy agar (TSA).
		MacConkeys agar is a medium that allows one to distinguish between lac+ and lac- bacteria.
	www.aug.edu /~bwellnitz/weekofApril1.htm (1580 words)

	Method and apparatus for the analysis of biological material - Patent 5624815
		Samples of 1.mu.l were removed from wells of the incubated filter microplate representing the mixed sample, and streaked onto MacConkey's agar.
		The remaining culture medium was removed under vacuum, and collected in a second microtitre plate for the enterotoxin assay.
		Table 2 summarises the spread plate results, representing the culture of 1.mu.l samples on MacConkey's agar from the mixed sample wells of the microtitre plate.
	www.freepatentsonline.com /5624815.html (4647 words)

	Candida Albicans Mycology on Blood Agar (Site not responding. Last check: 2007-10-18)
		Candida albicans on blood agar; moist, opaque colonies are characteristic of yeast.
		Candida albicans (Figures C, D) Candida albicans is a across the agar as hyphae at the colony margin Mycology.
		Subcultures were made on blood agar and MacConkeys agar.
	www.cindy-knows7.info /candida-albicans-mycology-on-blood-agar.html (757 words)

	Use of chlorate ion or preparations thereof for reduction of food borne pathogens - Patent 6761911
		Experiments were conducted both with cattle allowed continuous access to an alfalfa hay:flaked corn (9:1) diet, and with cattle that were fasted 24 hours prior to and after chlorate treatment.
		Concentrations of E. coli in the ruminal contents and in feces collected from the test animals were determined by viable cell counts on MacConkey agar plates.
		coli O157:H7 concentrations (log.sub.10 cfu/ml) were determined from colony counts on MacConkeys agar containing 25 ug/ml novobiocin and 20.mu.g/ml nalidixic acid, and S. typhimurium concentrations were determined from colony counts on Brilliant Green agar containing 25.mu.g/ml each of novobiocin and chloramphenicol.
	www.freepatentsonline.com /6761911.html (2894 words)

	TITLE: (Site not responding. Last check: 2007-10-18)
		The purpose of my project was to determine what temperature grew bacteria the fastest whether it was 0, 25, or 37 degrees Celsius; it was also to see which bacteria grew on MacConkeys and Blood agar.
		In conclusion to the growth on the different medias I found that E. coli and Pseudomonas aeruginosa were the only ones to grow on MacConkeys agar.
		Since MacConkeys is a selective media and gram-negative bacteria only grow on it that means E. coli and Pseudomonas are gram-negative.
	pilgrims.net /plymouth/schools/Science/scifair9/abstracts/biology/B42.htm (218 words)

	Culture, genital
		For genital cultures, we are wondering if it would be appropriate to bill for a Gardnerella screen (code 87081) along with code 87070.
		We are currently using five plates for our genital cultures: Chocolate, MacConkeys, Blood, GC-Lect, and HBT-Bilayer to check for Gardnerella.
		Recommendations regarding GC transport systems and have their been any comparisons between the JEMBEC and the Amies agar with charcoal transport swab in viability and recovery of the organisms within 24 to 48 hours.
	www.asm.org /Division/c/askit/culturegenital.htm (719 words)

	Detection of Salmonella in Foods by the Modified 1-2 Test
		C for 18-24 h, except for EF-18 agar, which is incubated at 42
		As needed, BIS and other agars are reincubated overnight.
		Colonies may have to be purified on MacConkeys agar or RAM.
	www.hc-sc.gc.ca /fn-an/res-rech/analy-meth/microbio/volume3/mflp70-04_e.html (431 words)

	National Institute of Oceanography, India (Site not responding. Last check: 2007-10-18)
		Samples were plated for total viable counts (TVC) on Nutrient agar; total coliforms (TC) on Macconkeys agar; Vibrio parahaemolyticus like organisms(VPLO) and Vibrio cholerae like organisms(VCLO) on thiosulphate citrate bile sucrose agar; Salmonella, Shigella, Proteus, and Klebsiella like organisms(SALO, SHLO, PKLO) on xylose lysine deoxycholate agar.
		Counts of all these groups were obtained after incubating the samples on the respective selective media at 35 + 2° C. Results of these groups are presented in Fig 1 and Tables 1 and 2.
		There could be many factors governing the survival and mortality of bacteria including physical (such as depth), chemical (dissolved oxygen, salinity, right types of nutrients, pH, dissolved organics, and toxicants) and biological (competition, phages etc).
	www.nio.org /past_events/comits/mar_pollut_poster.jsp (1379 words)

	Selective medium: Facts and details from Encyclopedia Topic (Site not responding. Last check: 2007-10-18)
		A selective medium is a substance (usually agar (Any culture medium that uses agar as the gelling agent)
		Eosin-methylen blue agar (EMB) that contains methylene blue which is toxic for Gram-positive (Gram-positive bacteria are those that are stained dark blue or violet by gram staining, in contrast to gram-negative...)
		MacConkey's Agar (Macconkeys agar is a culture medium designed to grow up gram-negative bacteria and stain them for lactose fermen...)
	www.absoluteastronomy.com /ref/selective_medium (966 words)

	some title (Site not responding. Last check: 2007-10-18)
		This is an enriched medium, used Mannitol salt agar, Pseudomonas agar MacConkeys agar, Saubourauds dextrose agar, selective for
		are grown in soft agar or in broth seeded with is analogous to the plate count of bacteria in which observed growing in the plaque.
		This is tryptic soy agar plus sheep red blood cells Amaranth* Soy Agar.
	myblog609.weblogs7.info /index.php?entry=entry051218-182641 (2107 words)

	Pakistan Pediatric Association - Jornal Section - Full (Site not responding. Last check: 2007-10-18)
		All procedure was done undr aseptic precautions to avoid contamination.
		In vitro antimicrobial sensitivity test was performed on the dignostic sensitivity agar platesBy the Kirby Bauer disc diffusion method.
		Commercial disc of following antibiotics were used for this study: Chloramphenicol, Ceftriaxone Ofloxacin, Mpiclox (ampicillin + clozacillin+Clavulanate.
	www.ppa.org.pk /journal/main/full.asp?article_NO=V24N4P127-133 (676 words)

	Verran - Student Handouts
		This is selective for streptococci which form a major part of the oral flora.
		Mannitol salt agar (MSA), Pseudomonas agar, (Ps), MacConkeys agar (Mac), Saubourauds dextrose agar (Sab), selective for staphylococci, pseudomonads coliforms and yeast respectively Aerobic incubation (10
		Wilkins-Chalgren agar (WC) with anaerobe selective supplement for obligate anaerobes (e.g.
	www.microbelibrary.org /edzine/Curriculum/Collection/VerranStudenthandout.htm (1046 words)

	QMJ
		Fluid specimens, including disinfectants, were centrifuged and the deposit cultured.
		All specimens were plated out directly onto MacConkeys agar and incubated overnight at 37
		Colonies consistent with B.cepacia were further identified using Vitek 2 (bioMerieux, France).
	www.hmc.org.qa /hmc/qmj/june2003/study/study3.htm (1575 words)

	Bacteriological Media (Site not responding. Last check: 2007-10-18)
		Beta Hemloysis on enriched agar - Blood agar
		Non-Hemloytic growth on enriched agar - Blood agar
		aureus on selective/differential - Mannitol Salt Agar (MSA)
	faculty.mc3.edu /jearl/ML/ml-8.htm (54 words)

	Introduction to Gastrointestinal Infections: Question 2 Choice 4 (Site not responding. Last check: 2007-10-18)
		What easy test detects Salmonella and/or Shigella in a stool sample?
		D) light color of colonies on EMB or MacConkeys agar plates
		Yes, Shigella and Salmonella cannot ferment the lactose in these two differential and selective media (Gram positives will not grow).
	medinfo.ufl.edu /cgi-bin/quiz.cgi?a56a/q2a4 (69 words)

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