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Topic: Maturation promoting factor

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	Maturation promoting factor - Wikipedia, the free encyclopedia
		Maturation promoting factor (abbreviated MPF, also called mitosis-promoting factor or M-Phase promoting factor) is a heterodimeric protein composed of cyclin B and cyclin-dependent kinase (CDK1, also known as Cdc2) that stimulates the mitotic and meiotic cell cycles.
		MPF promotes the entrance into mitosis from the G2 phase by phosphorylating multiple proteins needed during mitosis.
		MPF is disassembled when anaphase-promoting complex (APC) polyubiquitinates cyclin B, marking them for degradation.
	en.wikipedia.org /wiki/Maturation_promoting_factor (167 words)

	Maturation promoting factor - Indopedia, the Indological knowledgebase (Site not responding. Last check: 2007-11-01)
		Maturation promoting factor (abbreviated MPF, also called mitosis-promoting factor) is a heterodimeric protein composed of cyclin (cyclin B, a mitotic cyclin) and cyclin-dependent kinase (CDK1, also known as Cdc2) that stimulates the mitotic and meotic cell cycles.
		MPF promotes the entrance into mitosis from the G2 phase by phosphorylating multiple proteins involved in chromosome condensation and the breakdown of the nuclear envelope.
		MPF is disassembled when anaphase-promoting complex (APC) polyubiquitinates the mitotic cyclins, marking them for degradation.
	www.indopedia.org /MPF.html (216 words)

	The Lasker Foundation \| Former Award Winners, Basic Medical Research
		The discoverer of a protein in the cytoplasm of cells, called Maturation Promoting Factor (MPF), that controls cell division, Yoshio Masui was born in Kyoto, Japan, in 1931, the eldest son of Fusa-Jiro Masui, his father, and Toyoko Masui, his mother.
		MPF is now recognized as a ubiquitous factor that promotes mitosis, as well as cell division.
		Wasserman, W.J. and Masui, Y. 1976 A cytoplasmic factor initiatingmeiotic maturation in amphibian oocytes: Its extraction andpreliminary characterization.
	www.laskerfoundation.org /awards/library/1998b_int_other.shtml (1649 words)

	Milestone 7 Maturing with age
		During the 1960s, scientists were furiously searching for the factor that mediated this process, but it wasn't until 1971 that Yoshio Masui and Clement Markert identified the maturation-promoting factor (MPF).
		One of the main advances that allowed Masui and Markert to identify the mythical MPF was the use of frog oocytes to study the factors that induced mitosis and meiosis.
		MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B. PubMed
	www.nature.com /celldivision/milestones/full/milestone07.html (780 words)

	Biology Division - Dunphy's Home Page
		In dividing eukaryotic cells, a diverse family of cyclin-dependent kinases (Cdks) directs the progression of the cell cycle by regulating the accurate replication of the genome during S-phase and the faithful segregation of the chromosomes at mitosis (M-phase).
		MPF acts by phosphorylating a wide variety of substrates that collaborate in the execution of mitotic processes such as chromosome condensation, nuclear envelope breakdown, and spindle assembly.
		The activation of MPF is controlled by an intricate series of phosphorylation reactions on the Cdc2 subunit.
	biology.caltech.edu /Members/Dunphy (477 words)

	Interactive Fly, Drosophila
		MPF (M-phase promoting factor or maturation promoting factor), a key regulator of the G2-->M transition of the cell cycle, is a complex of cdc2 and a B-type cyclin.
		Analysis of the rate of maturation in hybrids obtained after fusion of nuclear and cytoplasmic fragments of oocytes from both strains suggests that both the germinal vesicle and the cytoplasm contain factor(s) influencing the length of the first meiotic M phase.
		Inactivation of MPF by the cdc2 kinase inhibitor roscovitine induces parthenogenetic activation.
	www.sdbonline.org /fly/newgene/cdc2-2c.htm (4256 words)

	RedOrbit - Science - Cyclic Nucleotide Phosphodiesterase 3A-Deficient Mice As a Model of Female Infertility
		Meiotic maturation in Pde3a^sup -/-^ oocytes was restored by inhibiting protein kinase A (PKA) with adenosine-3',5'-cyclic monophosphorothioate, Rp-isomer (Rp-cAMPS) or by injection of protein kinase inhibitor peptide (PKI) or mRNA coding for phosphatase CDC25, which confirms that increased cAMP- PKA signaling is responsible for the meiotic blockade.
		Moreover, murine oocyte maturation, induced by microinjection of an active PDE (3), is associated with decreases in cAMP (28, 29).
		Maturation of Pde3a^sup +/+^ and Pde3a^sup -/-^ oocytes in vivo and in vitro.
	www.redorbit.com /news/display?id=80066 (7159 words)

	Reproductive Biology and Endocrinology \| Full text \| Induction and inhibition of oocyte maturation by EDCs in zebrafish (Site not responding. Last check: 2007-11-01)
		Oocyte maturation in lower vertebrates is triggered by maturation-inducing hormone (MIH), which acts on unidentified receptors on the oocyte surface and induces the activation of maturation-promoting factor (MPF) in the oocyte cytoplasm.
		Oocyte maturation in fish is triggered by MIH, which acts on receptors located on the oocyte membrane and induces the activation of MPF in the oocyte cytoplasm [2].
		During the course of maturation, oocytes undergo drastic morphological changes associated with progression of the meiotic cell cycle, among which breakdown of the oocyte nuclear envelope (germinal vesicle breakdown, GVBD) occurring at the prophase/metaphase transition is usually regarded as a hallmark of the progress of oocyte maturation.
	www.rbej.com /content/3/1/69 (3849 words)

	oocyte maturation
		Resumption of meiosis is accompanied by the activation of maturation-promoting factor – a complex consisting of a regulatory protein, cyclin B2, and a catalytic protein, Cdc2.
		The activity of MPF is controlled by the association of these proteins with each other, and by two competing enzymes, the Myt1 kinase and Cdc25, that either add or remove phosphate groups from Cdc2.
		Active MPF is required for dissolution of the envelope surrounding the nucleus and formation of the 'spindle' apparatus that will segregate homologous chromosomes.
	www.vetscite.org /publish/items/000552 (1834 words)

	Dynamics of maturation-promoting factor and its constituent proteins during in vitro maturation of bovine oocytes -- Wu ...
		Dynamics of maturation-promoting factor and its constituent proteins during in vitro maturation of bovine oocytes -- Wu et al.
		Dynamics of maturation-promoting factor and its constituent proteins during in vitro maturation of bovine oocytes
		MPF is a complex of a B cyclin and the
	www.biolreprod.org /cgi/content/abstract/56/1/253 (850 words)

	Two Distinct Mechanisms Control the Accumulation of Cyclin B1 and Mos in Xenopus Oocytes in Response to Progesterone -- ...
		Maturation was monitored by the appearance of a white spot at the animal pole of the oocyte.
		MPF activity was measured by assaying histone H1 kinase activity (A and C), and an amount equivalent to three oocytes was subjected to Western blot analysis for cyclin B2 (B and D).
		response to progesterone in the absence of MPF activation.
	www.molbiolcell.org /cgi/content/full/10/10/3279 (6188 words)

	Cell Coupling and Maturation-Promoting Factor Activity in In Vitro-Matured Prepubertal and Adult Sheep Oocytes -- Ledda ...
		MPF fluctuations in prepubertal and adult oocytes during in vitro meiotic progression.
		Goudet G, Bézard J, Belin F, Duchamp G, Palmer E, Gérard N. Oocyte competence for in vitro maturation is associated with histone H1 kinase activity and is influenced by estrous cycle stage in the mare.
		Khatir H, Lonergan P, Mermillod P. Kinetics of nuclear maturation and protein profiles of oocytes from prepubertal and adult cattle during in vitro maturation.
	www.biolreprod.org /cgi/content/full/65/1/247 (3983 words)

	MCDB 4650 (Site not responding. Last check: 2007-11-01)
		In all eggs, the meiotic divisions are highly asymmetric with regards to apportioning the cytoplasm, since the mature egg itself needs to retain a large fraction of the original cytoplasm.
		MPF turns out to be involved in all cell divisions and now is also known as meiotic or mitotic promoting factor, still MPF.
		The mature egg is in a quiescent metabolic state.
	mcdb.colorado.edu /courses/4650/lecture2.html (1909 words)

	The Eukaryotic Cell Cycle and Cancer
		The accumulation of a specific cellular mass is a factor for somatic cells.
		The existence of such a complex was described biochemically when a factor called maturation promoting factor (MPF) was isolated that could initiate mitosis in certain mutant yeast strains whose cell cycle was arrested at this stage.
		It is clear from the genetic and biochemical studies that the appearance of an active MPF occurs at the M-phase committal point.
	www.ndsu.nodak.edu /instruct/mcclean/plsc431/cellcycle/cellcycl1.htm (1045 words)

	mcb_template.gif (Site not responding. Last check: 2007-11-01)
		We are also interested in investigating the role of Ca2+ in the activation of maturation promoting factor (MPF) in the same stage of meiosis.
		"Biochemical and developmental evidence that ooplasmic maturation of prepubertal bovine oocytes is compromised." Biol Reprod 64(6): 1761-8.
		Injection of sperm cytosolic factor into mouse Metaphase II oocytes induces different developmental outcomes according to the frequency of [Ca2+]i oscillations and oocyte age.
	www.bio.umass.edu /mcb/faculty/fissore.html (553 words)

	General Biology 111 (Site not responding. Last check: 2007-11-01)
		Promotion of synthesis of compounds needed for mitosis and cytokinesis, including proteins such as tubulin to form microtubules of spindle fibers.
		Mature nerve cells no longer divide, so they do not replicate their DNA.
		MPF would be present always and cell would continue to enter cell cycle.
	www.spelman.edu /~biology/bio111/bio111_rec_11_06.html (635 words)

	The Mitogen-Activated Protein Kinase Signaling Pathway Stimulates Mos mRNA Cytoplasmic Polyadenylation during Xenopus ... (Site not responding. Last check: 2007-11-01)
		the maturation of immature oocytes in the absence of exogenous
		MPF (cdc2-cyclin B) has been implicated in the induction of mRNA cytoplasmic polyadenylation (4, 15, 47, 58).
		MPF may functionally synergize with the feedback loop by acting to stabilize newly synthesized Mos protein (55) (dashed line), although this requirement can be overcome under conditions of robust MAPK activation (Fig.
	mcb.asm.org /cgi/content/full/19/3/1990 (7792 words)

	Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes -- Ma et al. 23 ... (Site not responding. Last check: 2007-11-01)
		Germinal vesicle oocytes were injected with MPF extracts (5 nl per oocyte) derived from metaphase II-arrested eggs and immediately placed in OR2 with or without cycloheximide (CHX).
		Extracts were subjected to MPF assays (upper panel) or immunoblotting with antibodies against cyclin B2 (middle panel) or MAP kinase (lower panel).
		MPF assays were carried out with extracts without prior fractionation, whereas Aurora-A kinase assays were carried out following immunoprecipitation (IP) with antibodies against Aurora-A. Shown is a representative of three independent experiments.
	mcb.asm.org /cgi/content/full/23/5/1703 (7453 words)

	Transcription Factor E2F and Cyclin E-Cdk2 Complex Cooperate to Induce Chromosomal DNA Replication in Xenopus Oocytes ...
		Transcription Factor E2F and Cyclin E-Cdk2 Complex Cooperate to Induce Chromosomal DNA Replication in Xenopus Oocytes -- Akamatsu et al.
		Oocyte maturation was induced to occur by stimulation of PG in the absence (
		MPF activation is governed by Mos proteins whose expressions are
	www.jbc.org /cgi/content/full/273/26/16494 (5445 words)

	Laura Hake - Boston College (Site not responding. Last check: 2007-11-01)
		This activates CPEB, which then recruits a complex of proteins, cleavage and polyadenylation specificity factor and poly A polymerase (CPSF and PAP), to the mRNA, and the poly A tail is elongated.
		In Xenopus oocytes, steroid hormones trigger a signal transduction cascade that activates at least two separate pathways that converge to activate maturation promoting factor (MPF), a powerful kinase that is the work horse of meiosis.
		Hake, L. E., and Richter, J. CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
	www.bc.edu /schools/cas/biology/facadmin/hake (1102 words)

	Cell Cycle Control (Site not responding. Last check: 2007-11-01)
		The “maturation promoting factor” (MPF) that releases frog eggs from G-2 arrest is a heterodimer of a cyclin and a cyclin-dependant kinase (cyclin/cdk).
		Several cyclin/cdks control progress through the cell cycle, each releasing an arrest at a specific “restriction point” in the cell cycle.
		This is mediated by the transcription factor p53.
	web.bio.utk.edu /bio140km/cell_cycle_control.htm (196 words)

	Professors in Retirement - Department of Zoology
		He and his colleagues showed that changes in nuclear activities during oocyte meiosis and mitosis are controlled by two protein factors in the cytoplasm, maturation promoting factor (MPF) and cytostatic factor (CSF).
		Masui’s microinjection technique enabled him to directly transfer into cells controlled amounts of maturation promoting factor that initiates cell division and the cytostatic factor that stops it, confirming their role in cell growth.
		In the 1990s, the focus of his lab has shifted from oocyte maturation and fertilization to cell cycle studies of early development.
	www.zoo.utoronto.ca /zfa/Newsletter-htm/april-98/retire.htm (1149 words)

	Modeling the Cell Division Cycle: cdc2 and Cyclin Interactions -- Tyson 88 (16): 7328 -- Proceedings of the National ...
		The proteins cdc2 and cyclin form a heterodimer (maturation promoting factor) that controls the major events of the cell cycle.
		Simulation and analysis of the model show that the control system can operate in three modes: as a steady state with high maturation promoting factor activity, as a spontaneous oscillator, or as an excitable switch.
		We associate the steady state with metaphase arrest in unfertilized eggs, the spontaneous oscillations with rapid division cycles in early embryos, and the excitable switch with growth-controlled division cycles typical of nonembryonic cells.
	www.pnas.org /cgi/content/abstract/88/16/7328 (303 words)

	MAP Kinase Is Required for the Spindle Assembly Checkpoint but Is Dispensable for the Normal M Phase Entry and Exit in ...
		In the extracts, activation of MAPKK and MAP kinase during the cell cycle occurred slightly behind that of MPF (Figs.
		Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
		Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
	www.jcb.org /cgi/content/full/136/5/1091 (5153 words)

	RALPH ALBRECHT SORENSEN
		Ph.D. Dissertation: "Problems in Oocyte Maturation and Early Development in the Mouse," Dr. Clement L. Markert, Advisor.
		"In Vitro Maturation of Mouse Oocytes Isolated from Late, Middle, and Pre-Antral Graafian Follicles." Journal of Experimental Zoology 190:123-127.
		"Active Maturation-Promoting Factor Is Present in Mature Mouse Oocytes." Journal of Cell Biology 100:1637-1640.
	www.gettysburg.edu /~rsorense/homepage/CV.htm (603 words)

	Whose end is destruction: cell division and the anaphase-promoting complex -- Zachariae and Nasmyth 13 (16): 2039 -- ... (Site not responding. Last check: 2007-11-01)
		SCF collaborates with several different substrate recognition subunits; Cdc4 which binds to the substrate Sic1 is shown as one example.
		The von Hippel-Lindau protein (pVHL) is thought to play a similar role in the VBC complex by recruiting the hypoxia-inducible factor (HIF).
		How the WD coactivators promote ubiquitination is currently unknown.
	www.genesdev.org /cgi/content/full/13/16/2039 (8884 words)

	New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation -- Hochegger et al. ...
		New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation -- Hochegger et al.
		New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation
		promoting factor (MPF) activity corresponding to meiosis I and
	dev.biologists.org /cgi/content/abstract/128/19/3795 (1024 words)

	c-mos and cdc2 Cooperate in the Translational Activation of Fibroblast Growth Factor Receptor-1 during Xenopus Oocyte ...
		c-mos and cdc2 Cooperate in the Translational Activation of Fibroblast Growth Factor Receptor-1 during Xenopus Oocyte Maturation -- Culp and Musci 10 (11): 3567 -- Molecular Biology of the Cell
		During oocyte maturation in Xenopus, previously quiescent maternal mRNAs are translationally activated at specific times.
		Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo
	www.molbiolcell.org /cgi/content/abstract/10/11/3567 (413 words)

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