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| | Primer3 Input Help |
 | | Primer3 uses the oligo melting temperature formula given in Rychlik, Spencer and Rhoads, Nucleic Acids Research, vol 18, num 21, pp 6409-6412 and Breslauer, Frank, Bloeker and Marky, Proc. |
 | | Primer3 uses the same salt concentration value for calculating both the primer melting temperature and the oligo melting temperature. |
 | | The value of this parameter is less than the actual concentration of oligos in the reaction because it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. |
| frodo.wi.mit.edu /cgi-bin/primer3/primer3_www_help.cgi (2848 words) |
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