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Topic: Methylene blue


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In the News (Mon 28 Dec 09)

  
  Molecular Research Center Methylene Blue Stain for RNA, DNA, nucleic acid analysis
Methylene Blue Stain is an aqueous solution of methylene blue designed for quantitative or qualitative examination of RNA and DNA immobilized on hybridization membranes in northern, Southern and alkaline blotting.
Methylene Blue Stain was tested and found applicable for use with both plastic and nitrocellulose membranes.
Methylene Blue Stain detects > 20 ng RNA or DNA/band.
www.mrcgene.com /met-blue.htm   (342 words)

  
  Olympus MIC-D: Polarized Light Gallery - Methylene Blue
The salt, methylene blue thiocyanate, is used as a redox indicator in the sanitary monitoring of milk.
Similarly, methylene blue is indicated as a remedy for cyanide poisoning.
Methylene blue is sometimes used in surgey as a dye for parts of the body the medical team wishes to identify.
www.olympusmicro.com /micd/galleries/polarized/methyleneblue1.html   (461 words)

  
 Methylene blue - Wikipedia, the free encyclopedia
While methylene blue is not as sensitive as ethidium bromide, it is less toxic and it does not intercalate in nucleic acid chains, thus avoiding interference with nucleic acid retention on hybridization membranes or with the hybridization process itself.
Methylene blue is used in endoscopic polypectomy as an adjunct to saline or epinephrine, and is used for injection into the submucosa around the polyp to be removed.
Methylene blue is used in aquaculture, particularly by tropical fish hobbyists, as a treatment for fish infected by the parasitic protozoa Ichthyophthirius multifilius (ich).
en.wikipedia.org /wiki/Methylene_blue   (918 words)

  
 Methylene Blue, Loefflers
Hardy Diagnostics Methylene Blue, Loefflers stain is a multipurpose stain for bacteria and leukocytes.
Methylene Blue, Loefflers is recognized as a simple stain used for determining bacterial morphology.
In addition to the staining of microorganisms, Methylene Blue, Loefflers was demonstrated by Harris in 1972 to be an effective stain for the enumeration of leukocytes in mucus or feces.
www.hardydiagnostics.com /catalog/hugo/MethyleneBlueLoefflers.htm   (882 words)

  
 KPD-28 Methylene Blue   (Site not responding. Last check: 2007-10-24)
Methylene Blue is safe for use with fish eggs and fry for the prevention of fungal infections.
Methylene Blue is not indicated for the treatment of Oodinium, bacterial infections, flukes (monogenetic trematodes) or for moderately-severe to severe fungal infections.
The use of Methylene Blue is primarily for the control of fungus on eggs, and to assist the transport of oxygen in fish poisoned by cyanide and nitrite ion.
www.novalek.com /kpd28.htm   (1092 words)

  
 METHEMOGLOBINEMIA   (Site not responding. Last check: 2007-10-24)
Methylene blue is a thiazine dye that may also be used to treat cyanide poisoning and urolithiasis.
Methylene blue is contraindicated in the patient that has a G6PD deficiency due to hemolysis and in those patients who have a severe renal impairment or a known hypersensitivity to the drug.
Dark blue lips and fingernails are the only traces of Martin Fugate's legacy left in the boy; that, and the recessive gene that has shaded many of the Fugates and their kin blue for the past 162 years.
www.mc.uky.edu /ahec/skyahec/methem-CE.htm   (4358 words)

  
 Methylene Blue Abolishes Slow Waves   (Site not responding. Last check: 2007-10-24)
Methylene blue, which can be somewhat specific histological stain for ICC under some conditions, depolarizes GI muscle preparations and blocks slow waves.
We were concerned about the large depolarization caused by methylene blue and argued that the compound must have additional effects in smooth muscles besides its obvious effects on ICC (see Sanders et al., Am.
Others have argued the effects of methylene blue are specific, and the reader might want to read the arguments in: Liu et al., Am.
www.physio.unr.edu /ICC/database/slide-3.htm   (177 words)

  
 Parathyroid Adenoma (MIRP)* OTOLARYNGOLOGY HOUSTON
The right sternocleidomastoid muscle was retracted.  The sternohyoid muscles were separated in the midline and retracted.  The dark blue adenoma immediately came into view, inferior to the right thyroid lobe and lateral to the trachea.
A preoperative infusion of methylene blue was employed in 20 patients undergoing neck exploration for hyperparathyroidism.
Methylene blue infusion is a safe method of more rapidly identifying parathyroid tissue.
www.ghorayeb.com /ParathyroidAdenomaMethyleneBlue.html   (209 words)

  
 methylene blue   (Site not responding. Last check: 2007-10-24)
Methylene Blue acts as an inhibitor of bacteria and fungi, and is well tolerated by most fish species, and toxic to certain sensitive scaleless species.
Methylene Blue is a redox dye which raises the oxygen consumption of cells This means the hydrogen of the materials to be oxidized is passed on to the oxygen.
Thus, while disinfection results from this, methylene blue is also an excellent agains methemoglobin intoxication.
www.argent-labs.com /methyleneblue.htm   (202 words)

  
 Methylene Blue (Methylene Blue Injection) drug description - prescription drugs and medications at RxList
Methylene Blue (Methylene Blue Injection) drug description - prescription drugs and medications at RxList
Methylene Blue Injection, USP is a sterile solution of Phenothiazin-5-ium,3,7-bis (dimethylamino)-, chloride, trihydrate.
Each mL contains 10 mg of Methylene Blue, USP in Water for Injection q.s.
www.rxlist.com /cgi/generic3/methyleneblue.htm   (90 words)

  
 Methylene blue test
The methylene blue test is a test to determine the type of methemoglobinemia an affected person has, based on the responsiveness of blood methemoglobin to the administration of methylene blue (a dark green powder that can turn methemoglobin back into normal hemoglobin).
Methylene blue is then injected into the vein.
Most methemoglobinemia quickly responds to IV administration of methylene blue, and can be controlled by daily doses of oral methylene blue and ascorbic acid.
www.umm.edu /ency/article/003412.htm   (520 words)

  
 MethyleneBlue
Methylene Blue should not be used for prolonged exposure, or in permanent (main, display) systems...
There is evidence that utilizing Methylene Blue in concentration in the process of acclimating marine livestock reduces toxicity of nitrite and cyanide.
Methylene Blue is relatively non-toxic in a very wide range of concentrations.
www.wetwebmedia.com /methblueart.htm   (549 words)

  
 Methylene Blue (Systemic)
Methylene blue is also used as a dye to stain certain parts of the body before or during surgery.
It is not known whether methylene blue passes into breast milk.
Although there is no specific information comparing the use of methylene blue in the elderly with use in other age groups, this medicine is not expected to cause different side effects or problems in older people than it does in younger adults.
healthqa.stbernards.info /html_healthgate/html/0_271/27129.php   (845 words)

  
 Optical Absorption of Methylene Blue   (Site not responding. Last check: 2007-10-24)
Methylene Blue can be activated by light to an excited state which in turn activates oxygen to yield oxidizing radicals.
This spectrum was taken with a HP spectrophotometer using a 1 cm quartz cuvette filled with 10µM solution of Methylene Blue in Water by Scott Prahl.
Tabulated data for the extinction coefficeint of methylene blue versus wavelength.
omlc.ogi.edu /spectra/mb   (228 words)

  
 methylene drug information, methylene side effects
Methylene blue is also used as a dye to stain certain...
Methylene chloride is predominantly used as a solvent...
Methylene Chloride, also known as dichloromethane and methylene dichloride, is a clear, colorless...
www.drugs.com /sch/methylene   (481 words)

  
 Stainsfile - Methylene blue
Methylene blue is a very commonly used dye.
It stains nuclei blue, and is often used for that purpose.
This solution is called polychrome methylene blue and is very valuable for staining rapid frozen sections.
stainsfile.info /StainsFile/dyes/52015.htm   (105 words)

  
 B028A - Methylene Blue Milk Experiments
So you would add 1 gram of methylene blue to 100 ml of water and autoclave that and then add 10 ml of that using a sterlie pipet or sterile graduate to to 90 ml of milk that you want to test.
I do not know the amount of methylene blue used in the old days when methylene blue was used to estimate the number of bacteria in milk.
Since the amount of blue color seen after incubation with methylene blue depends upon the number of bacteria and the kind of bacteria, I would change the objective of the project.
www.disknet.com /indiana_biolab/b028a.htm   (1170 words)

  
 Chemistry Kit Feeling Blue
When a solution of potassium hydroxide, dextrose and methylene blue is shaken, the oxygen dissolves into the colorless solution causing the methylene blue to oxidize into its blue form.
As the blue solution is left to stand, the solution turns colorless as the methylene blue is reduced.
Then, slowly the blue colored oxidized methylene blue is reduced by the glucoside and the solution turns back to colorless.
www.csun.edu /scied/2-chem/redox_methylene_blue   (553 words)

  
 The use of methylene blue as an extravascular surgical marker impairs vascular responses of human saphenous veins -- ...
The use of methylene blue as an extravascular surgical marker impairs vascular responses of human saphenous veins -- Barber et al.
The use of methylene blue as an extravascular surgical marker impairs vascular responses of human saphenous veins
of methylene blue is innocuous with regard to vascular function.
jtcs.ctsnetjournals.org /cgi/content/abstract/109/1/21   (372 words)

  
 MedlinePlus Drug Information: Methylene Blue (Systemic)   (Site not responding. Last check: 2007-10-24)
Allergies—Tell your doctor if you have ever had any unusual or allergic reaction to methylene blue.
Children—Babies are especially sensitive to the effects of methylene blue.
Kidney disease—In patients with kidney disease methylene blue may accumulate in the body; smaller doses of this medicine may be needed
www.nlm.nih.gov /medlineplus/druginfo/uspdi/202703.html   (961 words)

  
 Methylene Blue
TI: Reduction in formation of peritoneal adhesions by methylene blue in rats: a dose response study.
TI: The use of methylene blue as an extravascular surgical marker impairs vascular responses of human saphenous veins.
TI: Methylene blue as a cause of chemical peritonitis in a patient on peritoneal dialysis.
www.ubht.nhs.uk /library/methylene_blue.htm   (254 words)

  
 Methylene Blue Dye as an Alternative to Isosulfan Blue Dye for Sentinel Lymph Node Localization -- Simmons et al. 10 ...
Methylene Blue Dye as an Alternative to Isosulfan Blue Dye for Sentinel Lymph Node Localization -- Simmons et al.
Methylene blue as an alternative to isosulfan blue dye for sentinel lymph node localization.
A trend analysis of the relative value of blue dye and isotope localization in 2,000 consecutive cases of sentinel node biopsy for breast cancer.
www.annalssurgicaloncology.org /cgi/content/full/10/3/242   (2600 words)

  
 Methylene Blue Dye and Intestinal Atresia, NCBDDD, CDC
Methylene Blue Dye and Intestinal Atresia, NCBDDD, CDC
A CDC scientist reviewed the medical literature on the adverse fetal effects of the use of methylene blue (MB) dye during second trimester amniocentesis.
If the use of dye is felt to be unavoidable, alternative dyes, such as indigo carmine or Evans blue, should be considered.
www.cdc.gov /NCBDDD/bd/blue.htm   (388 words)

  
 Methylene Blue   (Site not responding. Last check: 2007-10-24)
METHYLENE BLUE is effective for the control of superficial fungal and bacterial infections of freshwater and marine fishes.
METHYLENE BLUE is also useful for the treatment of known nitrite and/or cyanide poisoning of aquarium fishes.
Caution: It has been determined that Methlene Blue will inhibit nitrification, resulting in increased concentration of ammonia in the water.
www.novalek.com /aqvtc9.htm   (278 words)

  
 Clinical Trial: Methylene Blue for Cognitive Dysfunction in Bipolar Disorder
The purpose of this study is to look at whether these symptoms can be improved by adding the compound methylene blue to the treatment plan of patients who are already taking lamotrigine.
Methylene blue is an available ‘over the counter medication’ in Canada.
This design is necessary because methylene blue stains urine and thus it is not possible to use a traditional placebo.
www.clinicaltrials.gov /ct/show/NCT00214877   (631 words)

  
 MedlinePlus Medical Encyclopedia: Methylene blue test
However, some types of hereditary methemoglobinemia do not respond to methylene blue.
Normally the IV administration of methylene blue rapidly lowers the levels of methemoglobin in the blood.
If the administration of IV methylene blue does not significantly lower blood levels of methemoglobin, then a rare form of hereditary methemoglobinemia is suspected.
www.nlm.nih.gov /medlineplus/ency/article/003412.htm   (526 words)

  
 Aquarium Products Treatments: Quick Cure, Clout, Neomycin, Methylene Blue.
Aquarium Products Treatments: Quick Cure, Clout, Neomycin, Methylene Blue.
To prepare a Methylene Blue bath; I use 1 teaspoon 2.303% solution per 5 gallons (double dose) in a bath of aquarium water from the tank the fish you wish to treat came from, I usually use about a ½ gallon of water, however you may use less.
Measurement of the Methylene Blue does not need to be precise as this bath should be used for about 30 minutes (although do NOT overdose).
www.americanaquariumproducts.com /Quickcure.html   (612 words)

  
 Methylene Blue
If a CSF Gram stain shows increased numbers of WBCs, but no bacteria are seen, a methylene blue stain should be performed.
Flood the air-dried, heat-fixed smear for 30 seconds with a 1% aqueous solution of methylene blue stain.
Gently rinse the slide with tap water and allow the slide to air dry or blot the slide with clean paper towel.
lab.chsys.org /default.aspx?id=50   (184 words)

  
 purevolume™ | Methylene Blue
The music of Methylene Blue is quickly spreading throughout lakeshore Wisconsin.
In creating a melodic folk-rock sound centered on dynamic musical arrangements and intricate vocal harmonies, the band has gained a solid reputation for its energetic live show.
As is apparent on their 2005 self-produced release Lies & Ambitions, Methylene Blue are continuing to grow musically and push their sound in new directions.
www.purevolume.com /methyleneblue/music   (120 words)

  
 Blister Tester automates methylene blue dye test., MOCON
Filled blister is secured at bottom of bowl and remains in methylene blue dye under atmospheric pressure for predetermined amount of time.
To use, a bowl-shaped chamber is filled with water to which the blue dye is added.
The blister packs remain in the methylene blue die under atmospheric pressure, for a predetermined amount of time.
news.thomasnet.com /fullstory/457812   (943 words)

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