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Topic: Microsatellite


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  Genetic Health - Colon Cancer: HNPCC - Microsatellite Instability Testing
Testing for microsatellite instability helps doctors determine whether a person is likely to have a gene mutation that causes hereditary nonpolyposis colorectal cancer (also known as HNPCC or Lynch Syndrome).
Tests are available that detect microsatellite instability in tumor cells, and finding numerous longer or shorter microsatellite regions in these cells suggests the presence of a mutated DNA mismatch repair gene — and that in turn points to HNPCC.
If a patient's tumor cells show no evidence of microsatellite instability, it's unlikely that he or she has a mutated mismatch repair gene — and that's precisely what genetic tests for HNPCC are looking for.
www.genetichealth.com /CRC_HNPCC_Microsatellite_Instability_Testing.shtml   (987 words)

  
  Microsatellite
Microsatellites are defined as short patterned non-coding regions containing specific genetic sequences that are repeated one right after the other (in direct sequence) within the genome of an organism.
The repeated sequence in a microsatellite consists of two, three or four nucleotides (di-, tri-, or tetra-nucleotide repeats respectively) and can be repeated many times (upwards of 100).
During the analysis of microsatellites in humans and other organisms it has become evident that the number of repeated sequences in a particular microsatellite locus can vary (location on the chromosome).
www.iscid.org /encyclopedia/Microsatellite   (249 words)

  
  Microsatellite - Wikipedia, the free encyclopedia
Microsatellite primers are developed by cloning random segments of DNA from the focal species.
Microsatellite loci are widely distributed throughout the genome and can be isolated from semi-degraded DNA of older specimens, as all that is needed is a suitable substrate for amplification through PCR.
Queller, D.C., Strassman,,J.E. and Hughes, C.R. "Microsatellites and Kinship".
en.wikipedia.org /wiki/Microsatellite   (1156 words)

  
 Microsatellite Analysis
Microsatellites (also called simple sequence repeats) are a class of genetic polymorphism commonly used for mapping, linkage analysis and to trace inheritance patterns.
The microsatellite is indicated in upper case letters, and the repeat unit is highlighted in red.
The region containing the microsatellite is amplified by PCR using primers (P1 and P2) that flank the microsatellite.
www.uvm.edu /~cgep/Education/Microsatellite.html   (497 words)

  
 Definition of microsatellite - NCI Dictionary of Cancer Terms
There is variation from person to person in the number of repeats.
There are hundreds of places in human DNA that contain microsatellites.
microsatellite instability, microscopic, microstaging, microwave acupuncture, microwave therapy
www.cancer.gov /Templates/db_alpha.aspx?CdrID=285938   (73 words)

  
 Microsatellite Markers
Akagi et al (1996) Microsatellite DNA markers for rice chromosomes.
Wu and Tanksley (1993) Abundance, polymorphism and genetic mapping of microsatellites in rice.
Wu and Tanksley (1993) Genetic and physical mapping of telomeres and microsatellites of rice.
www.gramene.org /microsat   (317 words)

  
 BioMed Central | Full text | A microsatellite-based consensus linkage map for species of Eucalyptusand a novel set of ...
Microsatellite comparative mapping data has also shown that genome homology across species of the same subgenus Symphyomyrtus is very high not only in terms of microsatellite flanking sequence conservation, but also marker order along linkage maps [30].
Although 70 microsatellites were mapped on a framework map of RAPD markers [15], 34 on a framework of AFLP markers [30], and 40 on a combined RFLP and candidate gene map [17], the genus Eucalyptus still lacks a more comprehensive genetic map built exclusively with microsatellite markers.
In a microsatellite transferability study between Quercus and Castanea, despite the high sequence identity at the flanking regions observed for 14 loci mapped in corresponding linkage groups, the repeat motif in the non-source species was in some cases shortened and/or modified [55].
www.biomedcentral.com /1471-2229/6/20   (8741 words)

  
 Miniaturized satellite - Wikipedia, the free encyclopedia
Microsatellite or "microsat" is usually applied to the name of an artificial satellite with a mass below 100 kg (220 lb).
Furthermore, since the overall cost risk in the mission is much lower, more up-to-date but less space-proven technology can be incorporated into micro and nanosats than can be used in much larger, more expensive missions with less appetite for risk.
SuitSat, a retired spacesuit fitted with some basic instrumentation and radio transmitting equipment and released into orbit in 2006, was an unconventional example of a low-cost microsatellite test platform.
en.wikipedia.org /wiki/Miniaturized_satellites   (685 words)

  
 The role of microsatellite instability in gastric carcinoma -- HAYDEN et al. 42 (2): 300 -- Gut
Prevalence of microsatellite instability and RER in gastric carcinomas
Microsatellite instability is associated with tumors that characterise the hereditary non-polyposis colorectal cancer syndrome.
Microsatellite instability in precancerous lesions and adenocarcinomas of the stomach.
gut.bmjjournals.com /cgi/content/full/42/2/300   (3486 words)

  
 Microsatellite Analysis More Sensitive
Microsatellite analysis is a more sensitive technique for the detection of bladder cancer cells in urine than conventional cytology.
Microsatellite analysis detected primary or recurrent tumours in 97 percent of the patients shown to have one of these conditions.
Indeed, microsatellite analysis increased the sensitivity of detecting grades G1-G2 bladder cancer from 75 to 95 percent.
www.annieappleseedproject.org /micanmorsen.html   (367 words)

  
 BioMed Central | Full text | Microsatellite polymorphism across the M. tuberculosisand M. bovisgenomes: Implications on ...
Microsatellites are the tandem repeats of nucleotide motifs of size 1–6 bp observed in all known genomes.
Microsatellites, also known as simple sequence repeats, are the short nucleotide segments comprising tandem repeating motifs of length 1–6 bp [1].
Microsatellite mutations are known to affect expression levels [6], switching on/off of genes [6] and even alteration of gene functions [7].
www.biomedcentral.com /1471-2164/7/78   (4658 words)

  
 Colon Cancer Patients With Genetic Abnormality Live Longer
Microsatellite instability is a type of genetic error found in the "letter" pairs that make up DNA, the basic set of instructions found in every cell of the body.
Microsatellite instability has been found in a high percentage of tumors that occur in people with hereditary nonpolyposis colorectal cancer (HNPCC), an inherited form of colon cancer in which multiple tumors occur at a young age.
Microsatellite instability refers to sections of mismatched DNA that fail to be repaired during cell division.
www.webmd.com /news/20000112/colon-cancer-genetic-abnormality-live-longer   (691 words)

  
 ARS | Publication request: Microsatellite DNA Marker-Assisted Selection of Saccharum Spontaneum Cytoplasm-Derived ...
Class H progeny inherited microsatellite alleles from both the S. spontaneum and the commercial-type parents and were, therefore, considered being F1 hybrids.
Class S and Class C progeny inherited microsatellite alleles only from one parent and were considered to be either selfs of either parent or F1 hybrids that only inherited allele(s) from one parent.
Class X progeny inherited non-parental microsatellite allele(s) in addition to the allele(s) from the maternal S. spontaneum parent and were considered to be contaminants.
www.ars.usda.gov /research/publications/publications.htm?seq_no_115=167835&pf=1   (648 words)

  
 Removal of Microsatellite Interruptions by DNA Replication Slippage: Phylogenetic Evidence from Drosophila -- Harr et ...
of an interruption in the microsatellite is an artifact of an
DMTOR to reconstruct the phylogeny of the microsatellite locus.
Microsatellite instability in yeast: dependence on the length of the microsatellite.
mbe.oxfordjournals.org /cgi/content/full/17/7/1001   (3058 words)

  
 I Was a Teenage Microsatellite - Student Worksheet
Microsatellites are patterned non-coding or junk DNA regions containing adjacent or tandem repeating sequences.
During the analysis of microsatellites in humans and other organisms it has become apparent that the number of repeated sequences in a particular microsatellite locus can vary (location on the chromosome).
Microsatellite differences are used in the DNA fingerprinting of individuals and has allowed ecologists to gather information on the variation that exists in populations of a species.
www.woodrow.org /teachers/esi/2002/Biology/Projects/p3/microsastudent.htm   (658 words)

  
 UTIAS/SFL - MOST Mission Objectives
The satellite's mission is to conduct long-duration stellar photometry observations in space.
To make these measurements, MOST incorporates into a microsatellite design a small (15 cm aperture), high-photometric-precision optical telescope and a Dynacon high performance attitude control system that is revolutionary in its pointing accuracy for a microsatellite.
Until recently, performing optical astronomy experiments from a low-cost microsatellite (mass < 100 kg) was considered unfeasible because of the poor pointing possible from a platform with such small inertia (approx.
www.utias-sfl.net /microsatellites/MOST/MOSTIndex.html   (682 words)

  
 Microsatellites--home
Please, please, _please_ stop using the old protocols.
We are now developing microsatellite DNA loci at SREL on a cost-reimbursement basis.
Many additional labs that develop microsatellite DNA loci are given on the Microsat-L web page.
www.uga.edu /srel/Msat_Devmt/Microsatellites--home.htm   (195 words)

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