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| | Cell Biology - Confocal Microscopy (Site not responding. Last check: 2007-11-07) |
 | | In confocal microscopy, as in conventional microscopy, it is necessary for the tissue specimen of interest to demonstrate some form of optical contrast between different areas of the sample for visualization |
 | | Two-photon, also referred to as multi-photon, microscopy setups are virtually the same as laser scanning confocal microscopy, except that the need for pinholes is eliminated. |
 | | Confocal microscopy is also used to analyze subcellular functions, such as pH gradients and membrane potentials, using specific fluorescent dyes and to measure intracellular changes in ion concentrations of molecules such as calcium, sodium, magnesium, zinc and potassium. |
| www.bioteach.ubc.ca /CellBiology/confocal (1736 words) |
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