| | Olympus FluoView Resource Center: Fluorescence Excitation and Emission Fundamentals |
 | | For this reason, emission is the mirror image of the ground state to lowest excited state transitions, but not of the entire absorption spectrum, which may include transitions to higher energy levels. |
 | | An excellent test of the mirror image rule is to examine absorption and emission spectra in a linear plot of the wavenumber (the reciprocal of wavelength or the number of waves per centimeter), which is directly proportional to the frequency and quantum energy. |
 | | Quinine does not adhere to the mirror image rule as is evident by inspecting the single peak in the emission spectrum (at 460 nanometers), which does not mirror the two peaks at 310 and 350 nanometers featured in the bimodal absorption spectrum. |
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