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NUMA1 - Nuclear mitotic apparatus protein 1 Nuclear Mitotic Apparatus protein (NuMA) is a 238-kDa protein of the nuclear matrix in interphase that relocates to the spindle poles in mitosis. NuMA (Nuclear protein that associates with the Mitotic Apparatus) is a 235-kD intranuclear protein that accumulates at the pericentrosomal region of the mitotic spindle in vertebrate cells. NuMA, a nuclear protein that associates with the mitotic apparatus, was identified in 1980 as a high molecular weight component of the nuclear matrix with the unusual property of associating with the microtubules of the spindle apparatus during mitosis. www.ihop-net.org /UniPub/iHOP/gi/90697.html   (3472 words)

CLB2 - G2/mitotic-specific cyclin 2 Recent data in yeast demonstrated that a partial degradation of the mitotic cyclin Clb2, mediated by APC/C and its activator protein Cdc20, is essential and sufficient for the mitotic exit. A mutation in Cdc28 (cdc28-1N) that interferes with Cks1 binding, or inactivation of Cks1 itself, confers stabilization of Clb2, the principal mitotic B-type cyclin in budding yeast. Destruction of the anaphase inhibitor Pds1p is necessary for separation of sister chromatids, whereas destruction of the mitotic cyclin Clb2p is important for disassembly of the mitotic spindle, cytokinesis and re-replication of the genome. www.ihop-net.org /UniPub/iHOP/gg/36245.html   (4730 words)

Association between Mitotic Spindle Checkpoint Impairment and Susceptibility to the Induction of Apoptosis by ... mitotic spindle checkpoint in the induction of apoptosis by for the induction of apoptosis are transduced in mitotic checkpoint-proficient The initial increases in the mitotic indices are similar regardless of the presence or absence of 200 nmol/L of nocodazole, indicating the absence of entry into mitosis in certain cell lines. ajp.amjpathol.org /cgi/content/full/163/3/1109   (4816 words)

The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1   (Site not responding. Last check: 2007-10-21) GST (lanes 3 and 4) or GST−Pin1 (lanes 5 and 6) beads were incubated in either interphase (lanes 3 and 5) or mitotic (lanes 4 and 6) extracts. Lane 1, mitotic extract; lane 2, mitotic extracts depleted with GST beads; lane 3, mitotic extracts depleted with Pin1 beads; lane 4, mitotic extracts depleted with GST beads and supplemented with recombinant Cdc25 (37 ng/ GST−Cyclin B was incubated in mitotic extracts and the resulting active Cdc2/cyclin B complex was retrieved on glutathione−Sepharose beads (note that this complex should be phosphorylated on Thr 161 with unlabeled phosphate). www.nature.com /emboj/journal/v17/n5/fig_tab/7590847ft.html   (2863 words)

ch-TOGp Is Required for Microtubule Aster Formation in a Mammalian Mitotic Extract -- Dionne et al. 275 (16): 12346 -- ... the mitotic spindle (22, 35, 38, 39) and indicates that Immunoprecipitation of the mammalian mitotic extract was performed with either a preimmune antibody (lane 1) or the ch-TOGp-specific antibody (lane 2), the resulting immunoprecipitates were separated by SDS-PAGE, and the proteins were identified by silver staining. The cell-free mitotic extract was processed for indirect immunofluorescence using antibodies specific for tubulin and NuMA (as indicated) following depletion with either a preimmune antibody (A and B) or the ch-TOGp-specific antibody (C and D). www.jbc.org /cgi/content/full/275/16/12346   (4438 words)

Polo-Like Kinase 1 Inactivation Following Mitotic DNA Damaging Treatments Is Independent of Ataxia Telangiectasia ... Dephosphorylation of Plk1 is correlated with mitotic arrest. Mitotic CA46 cells were either untreated or treated with 0.5 µmol/L of adriamycin for 1 hour. Mitotic CA46 cells were mock treated or pretreated with 2.5 mmol/L caffeine for 1 hour, after which 0.5 µmol/L adriamycin was added to medium for 1 hour. mcr.aacrjournals.org /cgi/content/full/2/7/417   (5580 words)

Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1 -- Poss et al. 18 (13): 1527 -- ... Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1 -- Poss et al. In 5 h, BrdU-positive cells (pink) result from mitotic division of spermatogonial stem cells to yield primary spermatocytes, or mitotic division plus one meiotic division to yield secondary spermatocytes. Cleveland, D.W., Mao, Y., and Sullivan, K.F. Centromeres and kinetochores: From epigenetics to mitotic checkpoint signaling. www.genesdev.org /cgi/content/full/18/13/1527   (3381 words)

A Mechanistic Model for the Organization of Microtubule Asters by Motor and Non-Motor Proteins in a Mammalian Mitotic ... The mammalian mitotic extract was treated with the Eg5 inhibitor monastrol (100 µM) or the protein kinase inhibitor staurosporine (1 µM) as indicated. The mammalian mitotic extract was depleted of Eg5 and/or supplemented with recombinant NuMA as indicated. Dionne, M.A., Howard, L., and Compton, D.A. NuMA is a component of an insoluble matrix at mitotic spindle poles. www.molbiolcell.org /cgi/content/full/15/5/2116   (8216 words)

Regulation of Complex Formation of POB1/Epsin/Adaptor Protein Complex 2 by Mitotic Phosphorylation -- Kariya et al. 275 ... The lysates from interphase (I, lanes 1 and 3) and mitotic phase (M, lanes 2 and 4) of CHO-IR cells were probed with the anti-Eps15, anti-RalBP1, anti-Epsin, and anti-POB1 antibodies (lanes 1 and 2) or precipitated with GST-app (lanes 3 and 4). A, mitotic phosphorylation of the C-terminal region of Epsin and POB1. The lysates of CHO-IR cells from interphase (I) or mitotic phase (M) expressing Myc-hEpsin were overlaid with GST (lanes 1 and 2) or GST-POB1-(126-227) (lanes 3-6). www.jbc.org /cgi/content/full/275/24/18399   (4944 words)

Clathrin in mitotic spindles -- Okamoto et al. 279 (2): 369 -- AJP - Cell Physiology Spindles were isolated from mitotic CV-1 cells according to a protocol adapted from Zieve et al. Association of clathrin with the mitotic spindle is not affected by brefeldin A (BFA). The localization of clathrin light chain to the mitotic spindle is not affected by BFA (arrowheads). ajpcell.physiology.org /cgi/content/full/279/2/C369   (3326 words)

Immunocytochemical assessment of mitotic activity with an antibody to phosphorylated histone H3 in the macaque and ... Immunocytochemical assessment of mitotic activity with an antibody to phosphorylated histone H3 in the macaque and human endometrium -- Brenner et al. Immunocytochemical assessment of mitotic activity with an antibody to phosphorylated histone H3 in the macaque and human endometrium In (c) a mitotic cell is evident in the stroma. humrep.oxfordjournals.org /cgi/content/full/18/6/1185   (4839 words)

The C-terminus of Bfa1p in budding yeast is essential to induce mitotic arrest in response to diverse ... mitotic exit in the absence of Bub2p (Ro et al. The expression of Bfa1p and its 13 deletion mutants was verified and the ability of each mutant to maintain mitotic arrest in response to spindle damage, spindle misorientation, and DNA damage as well as to localize to the SPB and to induce mitotic arrest by over-expression was examined. Li, Y., Bachant, J., Alcasabas, A.A., Wang, Y., Qin, J. and Elledge, S.J. (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. www.genestocellsonline.org /cgi/content/full/9/5/399   (7272 words)

Mitotic spindle -- Facts, Info, and Encyclopedia article   (Site not responding. Last check: 2007-10-21) Mitotic spindle -- Facts, Info, and Encyclopedia article It consists of a bundle of (A microscopically small tubule) microtubules joined at the ends but spread out in the middle, vaguely resembling an American football in shape. In most cells, the spindle is formed by the (Small region of cytoplasm adjacent to the nucleus; contains the centrioles and serves to organize the microtubules) centrosomes as they move apart. www.absoluteastronomy.com /encyclopedia/m/mi/mitotic_spindle.htm   (82 words)

Reliability of whole crypt mitotic count as a measure of cellular proliferation in rectal biopsies -- Tosteson et al. 5 ... Reliability of whole crypt mitotic count as a measure of cellular proliferation in rectal biopsies -- Tosteson et al. Reliability of whole crypt mitotic count as a measure of cellular proliferation in rectal biopsies count and the mean height of mitotic cells (r = 0.12). cebp.aacrjournals.org /cgi/content/abstract/5/6/437   (284 words)

The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins -- Shen et ... a subset of mitotic phosphoproteins on phosphorylated Ser/Thr-Pro The identification of the novel human mitotic regulator Pin1 suggests a new regulatory mechanism for mitotic regulation (Lu et al. Mitotic GST-Cdc25 was purified by incubating 22 µg of GST-Cdc25 in a Xenopus mitotic extract for 30 min at 23°C; this was www.genesdev.org /cgi/content/full/12/5/706   (7552 words)

The Kinesin-related Protein, HSET, Opposes the Activity of Eg5 and Cross-links Microtubules in the Mammalian Mitotic ... The total values were averaged over the number of sections, and the average number of gold particles and microtubules plotted as a function of the region of the spindle. HSET is required for the formation and maintenance of microtubule asters in a cell free mitotic extract. The formation of microtubule asters was stimulated by the addition of taxol, ATP, and by incubation at 31°C. The resulting structures were analyzed by indirect immunofluorescence using antibodies specific for tubulin and NuMA, as indicated. www.jcb.org /cgi/content/full/147/2/351   (7973 words)

Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae -- Myung ... Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae -- Myung et al. and microtubule tension are sensed by the mitotic checkpoints. missegregation caused by mutations in mitotic checkpoint and www.pnas.org /cgi/content/full/101/45/15980   (4601 words)

Mos positively regulates Xe-Wee1 to lengthen the first mitotic cell cycle of Xenopus -- Murakami et al. 13 (5): 620 -- ... The first mitotic cell cycle was initiated by treating unfertilized eggs with calcium ionophore (A23187). Drosophila wee1 kinase rescues fission yeast from mitotic catastrophe and phosphorylates Drosophila cdc2 in vitro. Mitotic and G2 checkpoint control: Regulation of 14-3-3 protein binding by phosphorylation of Cdc25c on Serine-216. www.genesdev.org /cgi/content/full/13/5/620   (6752 words)

Negative Regulation of Mitotic Promoting Factor by the Checkpoint Kinase Chk1 in Simian Virus 40 Lytic Infection -- ... Induction of mitotic markers and morphology in SV40-infected cells incubated with UCN-01. Induction of mitotic markers and morphology in SV40-infected CV-1 cells incubated with caffeine. A mitotic control was prepared from uninfected CV-1 cells arrested by nocodazole (lane N). jvi.asm.org /cgi/content/full/77/2/1257   (7103 words)

Gene Expression and Mitotic Exit Induced by Microtubule-Stabilizing Drugs -- Chen et al. 63 (22): 7891 -- Cancer ... with the absence of apoptosis in mitotic cells, survivin was mitotic kinase cdc2, leading to stabilization of survivin (36). of cells in mitotic slippage is accompanied by decreased expression cancerres.aacrjournals.org /cgi/content/full/63/22/7891   (6242 words)

Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit -- Luca et al. 21 (20): 6972 -- Molecular ... Esteban, V., Blanco, M., Cueille, N., Simanis, V., Moreno, S., Bueno, A. A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast. Ufano, S., Pablo, M. E., Calzada, A., del Rey, F., Vazquez de Aldana, C. Swm1p subunit of the APC/cyclosome is required for activation of the daughter-specific gene expression program mediated by Ace2p during growth at high temperature in Saccharomyces cerevisiae. Cid, V. J., Jimenez, J., Molina, M., Sanchez, M., Nombela, C., Thorner, J. Orchestrating the cell cycle in yeast: sequential localization of key mitotic regulators at the spindle pole and the bud neck. mcb.asm.org /cgi/content/abstract/21/20/6972   (747 words)

Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases -- Giet and Prigent 112 (21): ... to the mitotic apparatus: in the centrosome, at the poles of the bipolar The mitotic serine threonine kinase, Aurora-2, is a potential target for drug development in human pancreatic cancer The S. pombe aurora-related kinase Ark1 associates with mitotic structures in a stage dependent manner and is required for chromosome segregation jcs.biologists.org /cgi/content/abstract/112/21/3591   (1962 words)

Survivin Deregulation in {beta}-Tubulin Mutant Ovarian Cancer Cells Underlies Their Compromised Mitotic Response to ...   (Site not responding. Last check: 2007-10-21) Survivin Deregulation in {beta}-Tubulin Mutant Ovarian Cancer Cells Underlies Their Compromised Mitotic Response to Taxol -- Zhou et al. Survivin Deregulation in ß-Tubulin Mutant Ovarian Cancer Cells Underlies Their Compromised Mitotic Response to Taxol the mitotic response of the resistant cells to Taxol treatment. cancerres.aacrjournals.org /cgi/content/abstract/64/23/8708   (254 words)

BUBR1 Phosphorylation Is Regulated during Mitotic Checkpoint Activation -- Li et al. 10 (11): 769 -- Cell Growth & ... BUBR1 Phosphorylation Is Regulated during Mitotic Checkpoint Activation -- Li et al. BUBR1 Phosphorylation Is Regulated during Mitotic Checkpoint Activation p53 deficiency and defective mitotic checkpoint in proliferating T lymphocytes increase chromosomal instability through aberrant exit from mitotic arrest cgd.aacrjournals.org /cgi/content/abstract/10/11/769   (418 words)

Involvement of Nucleocytoplasmic Shuttling of Yeast Nap1 in Mitotic Progression -- Miyaji-Yamaguchi et al. 23 (18): ... Nucleocytoplasmic shuttling of yNap1 is involved in mitotic progression. NAP-I is a functional homolog of TAF-I that is required for replication and transcription of the adenovirus genome in a chromatin-like structure. NAP1 acts with Clb2 to perform mitotic functions and to suppress polar bud growth in budding yeast. mcb.asm.org /cgi/content/full/23/18/6672   (6853 words)

Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2 -- Höfken and Schiebel 164 (2): 219 -- The ... Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2 -- Höfken and Schiebel 164 (2): 219 -- The Journal of Cell Biology Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2 for mitotic exit when activation of the mitotic exit network www.jcb.org /cgi/content/abstract/164/2/219   (243 words)

Genetic and Biochemical Evaluation of the Importance of Cdc6 in Regulating Mitotic Exit -- Archambault et al. 14 (11): ... Genetic and Biochemical Evaluation of the Importance of Cdc6 in Regulating Mitotic Exit -- Archambault et al. with Sic1 and binds specifically to the mitotic cyclin Clb2. Quantitative Characterization of a Mitotic Cyclin Threshold Regulating Exit from Mitosis www.molbiolcell.org /cgi/content/abstract/14/11/4592   (416 words)

Mechanistic Analysis of the Mitotic Kinesin Eg5 -- Cochran et al. 279 (37): 38861 -- Journal of Biological Chemistry Mechanistic Analysis of the Mitotic Kinesin Eg5 -- Cochran et al. Articles by Cochran, J. Articles by Gilbert, S. Mechanistic Analysis of the Mitotic Kinesin Eg5 Docking and Rolling, a Model of How the Mitotic Motor Eg5 Works www.jbc.org /cgi/content/abstract/279/37/38861   (364 words)

The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M ... is sufficient to stop mitotic cycle and promote precocious endocycles Notch-dependent downregulation of the homeodomain gene cut is required for the mitotic cycle/endocycle switch and cell differentiation in Drosophila follicle cells The Cyclin-Dependent Kinase Inhibitor KRP2 Controls the Onset of the Endoreduplication Cycle during Arabidopsis Leaf Development through Inhibition of Mitotic CDKA;1 Kinase Complexes dev.biologists.org /cgi/content/abstract/131/13/3169   (333 words)

Potential Link between the NIMA Mitotic Kinase and Nuclear Membrane Fission during Mitotic Exit in Aspergillus nidulans ... Potential Link between the NIMA Mitotic Kinase and Nuclear Membrane Fission during Mitotic Exit in Aspergillus nidulans -- Davies et al. Potential Link between the NIMA Mitotic Kinase and Nuclear Membrane Fission during Mitotic Exit in Aspergillus nidulans N-TINC results in premature NIMA disappearance in mitotic ec.asm.org /cgi/content/abstract/3/6/1433   (290 words)

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