Where results make sense

About us   |   Why use us?   |   Reviews   |   PR   |   Contact us

Myoblast - Wikipedia, the free encyclopedia Myoblasts are a type of stem cells that exist in muscles. Skeletal muscle cells are called muscle fibers and are made when myoblasts fuse together; muscle fibers therefore have multiple nuclei. Myoblasts that do not form muscle fibers differentiate into satellite cells. en.wikipedia.org /wiki/Myoblast   (102 words)

Nikon MicroscopyU: Fluorescence Microscopy Digital Image Gallery - Embryonic Rat Thoracic Aorta Medial Layer Myoblast ... The myoblast cell line A-10 was established from tissue excised from the medial layer of the thoracic aorta of a rat embryo (Rattus norvegicus; DB1X strain). The A-10 myoblast cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 488 conjugated to phalloidin (green fluorescence), and for nuclear DNA with the bis-benzimidazole dye Hoechst 33258 (blue fluorescence). Five Color Fluorescence in A-10 Myoblast Cells - A culture of A-10 cells was immunofluorescently labeled with a combination of primary anti-vinculin mouse and anti-giantin rabbit monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 750 and goat anti-rabbit secondary antibodies conjugated to Alexa Fluor 568. www.microscopyu.com /galleries/fluorescence/cells/a10/a10.html   (581 words)

Skeletal muscle LIM protein 1 regulates integrin-mediated myoblast adhesion, spreading, and migration -- Robinson et ... Alternatively, HA-SLIM1-transfected myoblasts were grown in the presence of 10% FCS for 18 h and plated on fibronectin for 3 h with FCS (+FCS). C: HA-SLIM1-transfected myoblasts were plated on fibronectin in the absence or presence of RGD peptide (50 or 500 µg/ml) as indicated, and the percentage of transfected cells demonstrating HA-SLIM1 in focal adhesions, or in the nucleus, was determined. The alpha 7 beta 1 integrin mediates adhesion and migration of skeletal myoblasts on laminin. ajpcell.physiology.org /cgi/content/full/284/3/C681   (6609 words)

Johns Hopkins Biochemistry, Cellular and Molecular Biology Graduate Program   (Site not responding. Last check: 2007-10-31) My lab is using myoblast fusion in Drosophila as a model system to study the general mechanisms of cell-cell fusion. Myoblast fusion, the process in which mononucleated myoblasts fuse to form multinucleated muscle fibers, is a characteristic step during myogenesis. Given the evolutionary conservation of many signaling cascades, studies of myoblast fusion in Drosophila are likely to provide insights into mammalian myogenesis, and may have implications for therapeutic approaches to human myopathies. biolchem.bs.jhmi.edu /bcmb/Faculty_person.asp?PersonID=1009   (434 words)

Dynamics and mediators of acute graft attrition after myoblast transplantation to the heart -- SUZUKI et al. 18 (10): ... is crucial for the therapeutic efficacy of myoblast transplantation To study the role of oxidative stress in myoblast death, CuZn-SOD was administered in myoblast suspension. Surviving and total myoblast numbers were significantly increased by 10 min, with additional improvement at 24 and 72 h compared with untreated myoblasts (see Fig. www.fasebj.org /cgi/content/full/18/10/1153   (1665 words)

Three Year Phase I Myoblast Cell Transplantation Study Data Presented at American College of Cardiology Scientific ... The purpose of this multicenter study was to demonstrate the safety and feasibility of using autologous myoblast transplantation to repair damaged heart tissue in patients who have suffered a heart attack. In the study, myoblasts were harvested from the leg muscles of 30 patients and then cultured using GenVec’s proprietary technique. The results indicate that myoblast transplantation is feasible and safe in patients who have suffered myocardial infarction and are scheduled to undergo coronary artery bypass surgery or receive a LVAD, and that this approach to repairing damaged heart muscle warrants further study. www.genvec.com /go.cfm?do=Press.View&prid=217   (479 words)

MYOBLAST.ORG - ARIZONA HEART INSTITUTE AND HOSPITAL DEVELOPING NEW HEART MUSCLE FROM SKELETAL MUSCLE CELLS   (Site not responding. Last check: 2007-10-31) On March 3, 2005, physicians at the Arizona Heart Institute and Hospital used the NOGA mapping technology again to track the progress of the cell growth in Dr. Andrus' heart. Myoblast refers to the super "powerhouse" cells that originate from the human body's muscle stem cells. The trial is being completed in collaboration with Genvec,Inc. (www.genvec.com), a company that develops transplantable cells to treat disease characterized by cell dysfunction or death. www.myoblast.org   (677 words)

Modulation of Myoblast Fusion by Caveolin-3 in Dystrophic Skeletal Muscle Cells: Implications for Duchenne Muscular ... that overexpression of caveolin-3 inhibits myoblast fusion to to mediate the fusion of myoblasts to myotubes. overexpression of caveolin-3 inhibits myoblast fusion to multinucleated www.molbiolcell.org /cgi/content/full/14/10/4075   (7246 words)

Growth factor stimulation of matrix metalloproteinase expression and myoblast migration and invasion in vitro -- Allen ... B: myoblast migration assayed as described in A, except that 0.05 U/ml purified human plasmin or 50 mM N-acetyl cysteine (NAC) was added to the top chamber with the cells at the start of the assay. MMP overexpression and mouse myoblast migration and invasion. Transfection of mouse myoblasts with MMP-1 or MMP-2 increased the migration of mouse myoblasts in response to fibronectin ajpcell.physiology.org /cgi/content/full/284/4/C805   (6202 words)

Increase Muscle Build with MyoBlast Creatine Supplement MyoBlast is produced in an FDA approved and inspected laboratory with only the purest natural ingredients available worldwide, ensuring that each supplement will not contain less than 100% of the label claim. High-Performance MyoBlast consists of a superior grade of Creatine Monohydrate, a natural chemical that is muscles' main energy source. Since MyoBlast works by enhancing muscle cell hydration, it is very important to consume adequate fluids while taking this supplement to achieve best results. www.1stplace-supplements.com /creatine.phtml   (668 words)

Rotation Report Primary murine myoblast cell C57 and mdx myoblast cells were maintained in F12 medium supplemented with 20% heat-inactivated fetal calf serum and 1% penicillin-streptomycin antibiotic mixture (growth media). The mdx myoblast and C57 myoblast were replaced in the differentiated media as described in the Material and Methods. Otherwise the percentage of real mdx myoblasts in the culture dishes needs to be verified as the primary mdx cells potentially are cultures with some fibroblast. www.pitt.edu /~may9/report.htm   (2219 words)

[No title] Although many factors have been shown to be involved in these processes, the precise mechanism of myoblast fusion is still unclear ÁÁRecent data indicated that ADAM12, a newly identified member of the ADAM family of proteins (containing ÃÃAÄÄ ÃÃDÄÄisintegrin ÃÃAÄÄnd ÃÃMÄÄetalloprotease domain), might be involved in myoblast fusion. The main hypothesis is that the binding of the ADAM12 extracellular disintegrin and cysteine©rich domains to a specific receptor mediates the interactions between myoblasts. To investigate the role of À Àfusion peptideÀÀ in mediating myoblast fusion by overexpressing mutated ADAM12 in C2C12 cells, with disrupted helical structure of the fusion peptide. www.oznet.ksu.edu /SarachekAward/2002/sampleDocuments.doc   (1619 words)

Drosophila SNS, a member of the immunoglobulin superfamily that is essential for myoblast fusion -- Bour et al. 14 ... Drosophila myoblast city encodes a conserved protein that is essential for myoblast fusion, dorsal closure and cytoskeletal organization. Myoblast city, the Drosophila homologue of DOCK180/CED-5, is required in a Rac signaling pathway utilized for multiple developmental processes. Fusion from myoblasts to myotubes is dependent on the rolling stone gene (rost) of Drosophila. www.genesdev.org /cgi/content/full/14/12/1498   (6946 words)

The MyoD-Inducible p204 Protein Overcomes the Inhibition of Myoblast Differentiation by Id Proteins -- Liu et al. 22 ... myoblasts to differentiate is by overcoming the inhibition of Extracts prepared from C2C12 myoblasts in DM for 1 day were immunoprecipitated with control IgG (lane 3) or antiserum to Id2 (lane 2), Id1 (lane 4), or Id3 (lane 5). from the nucleus to the cytoplasm during myoblast fusion (47). mcb.asm.org /cgi/content/full/22/9/2893   (10438 words)

Human Myoblast Fusion Requires Expression of Functional Inward Rectifier Kir2.1 Channels -- Fischer-Lougheed et al. 153 ... Myoblasts transfected with the antisense RNA vectors were induced Human myoblasts were transfected with bicistronic vectors expressing antisense RNA to block expression of Kir2.1 (AS Kir2.l, triangles) or Kir2.2 (AS Kir2.2, circles), and IK density were recorded 2–3 d after the treated cells were transferred into DM to induce their differentiation. Myoblasts fusion is impeded when Kir2.1 expression is blocked by an antisense RNA vector. www.jcb.org /cgi/content/full/153/4/677   (6162 words)

University of Maryland Researchers Study Cell Transplantation to Repair Damaged Heart Muscle For this study, a Phase 1 clinical trial to evaluate the safety of the treatment, the myoblast transplantation is done in combination with coronary artery bypass grafting (CABG) in patients who have had a heart attack, and whose ejection fraction, a measure of the heart’s pumping ability, is less than 40 percent. The myoblasts begin their journey to the heart when a thimble-sized sample of muscle from the patient’s thigh is taken about six weeks before the CABG surgery. Because the myoblasts come from the patient and go back to the same patient, this procedure, called an autologous transplant, avoids the risk of graft rejection. www.umm.edu /news/releases/myoblast.html   (802 words)

RhoA GTPase Regulates M-Cadherin Activity and Myoblast Fusion -- Charrasse et al. 17 (2): 749 -- Molecular Biology of ... Cell lysates of control and RhoAV14-expressing C2C12 myoblasts collected at different times after addition of differentiation medium were immunoprecipitated using either an anti-M-cadherin (bottom left) or anti-N-cadherin antibody (bottom right) and immunoblotted for the presence of p120 catenin. we observed at the onset of myoblast fusion. activity at the onset of myoblast fusion remain to be determined. www.molbiolcell.org /cgi/content/full/17/2/749   (5160 words)

The Mitogen-activated Protein Kinase Cascade Promotes Myoblast Cell Survival by Stabilizing the Cyclin-dependent Kinase ... Myoblasts in 6-cm TC dishes (Corning) were transfected with a total amount of 10 In proliferating myoblasts the inactive pro-caspase was the in the survival of differentiating myoblasts (11, 13). www.jbc.org /cgi/content/full/278/23/21221   (6828 words)

Human Myoblast Differentiation: Ca2+ Channels are Activated by K+ Channels -- Bernheim and Bader 17 (1): 22 -- ... We observed that the fusion-competent myoblasts that are hyperpolarized D: myoblast fusion is reduced by an antisense (AS) probe directed against Kir2.1 but not by an antisense probe against Kir2.2. myoblasts [steep inward rectification and insensitivity to intracellular physiologyonline.physiology.org /cgi/content/full/17/1/22   (3552 words)

FoxO1a-Cyclic GMP-Dependent Kinase I Interactions Orchestrate Myoblast Fusion -- Bois et al. 25 (17): 7645 -- Molecular ... myoblasts display excessive fusion compared to control myoblasts and are similar to myoblasts engineered to overexpress dominant-active FoxO1a (5). Involvement of cyclic GMP in the fusion of chick embryonic myoblasts in culture. Inactivation of Rho/ROCK signalling is crucial for the nuclear accumulation of FKHR and myoblast fusion. mcb.asm.org /cgi/content/full/25/17/7645   (4560 words)

Human Primary Myoblast Cell Cultures from Non-Diabetic Insulin Resistant Subjects Retain Defects in Insulin Action -- ... Insulin resistance, or a reduced rate of insulin-stimulated glucose uptake is considered a defect of skeletal muscle (1). Primary myoblast cultures have been used to study normal muscle development as well as muscle diseases such as muscular dystrophy is intrinsic to myoblasts and is maintained in culture. www.jci.org /cgi/content/full/98/10/2346   (2932 words)

Muscle Morphogenesis: The Process of Embryonic Myoblast Fusion   (Site not responding. Last check: 2007-10-31) This process cannot be initiated until the myoblasts have differentiated to a point at which they become competent to fuse and express genes associated with the fusion process. The myoblasts must then identify and adhere to their fusion partners, a process that is seeded by the founder cell and involves its recruitment of fusion-competent myoblasts. While little is known at present about the actual molecules or process by which the lipid bilayers break down, it seems likely that known proteins essential for fusion will provide an entry into their identification. www.eurekah.com /abstract.php?chapid=2064&bookid=162&catid=20   (313 words)

Muscular Dystrophy: Encyclopedia of Medicine Two experimental procedures aiming to cure DMD have attracted a great deal of attention in the past decade. In myoblast transfer, millions of immature muscle cells are injected into an affected muscle. The goal of the treatment is to promote the growth of the injected cells, replacing the defective host cells with healthy new ones. health.enotes.com /medicine-encyclopedia/muscular-dystrophy   (4175 words)

Normal myoblast fusion requires myoferlin -- Doherty et al. 132 (24): 5565 -- Development adhesion and fusion of myoblasts (Chen and Olson, 2004 (A) Myoblasts isolated from littermate control and myoferlin null neonatal mice were plated at equal densities and induced to differentiate. Higher magnification images (scale bar: 50 µm) show an increase in the number of centrally placed nuclei, which is indicative of recent fusion, in wild type compared with in myoferlin null muscle. dev.biologists.org /cgi/content/full/132/24/5565   (6491 words)

Drosophila myoblast city Encodes a Conserved Protein That Is Essential for Myoblast Fusion, Dorsal Closure, and ... Drosophila myoblast city Encodes a Conserved Protein That Is Essential for Myoblast Fusion, Dorsal Closure, and Cytoskeletal Organization -- Erickson et al. The Drosophila myoblast city (mbc) locus was previously identified on the basis of a defect in myoblast fusion (Rushton et Activation of myosin synthesis in fusing and mononucleated myoblasts. www.jcb.org /cgi/content/full/138/3/589   (6790 words)

Lamin A N-terminal phosphorylation is associated with myoblast activation: impairment in Emery-Dreifuss muscular ... Lamin A N-terminal phosphorylation is associated with myoblast activation: impairment in Emery-Dreifuss muscular dystrophy -- Cenni et al. C2C12 myoblast lysates treated or not with alkaline phosphatase (100 U/ml) were subjected to western blot analysis by SW2-30 monoclonal antibody or antilamin A/C polyclonal antibody. Figure 2  N-terminus lamin A phosphorylation is observed in cyling C2C12 myoblasts (cyc), it is strongly reduced in resting myoblasts (rest), while it is preserved in differentiating myotubes at days 2 (2d) or 4 (4d) of differentiation. jmg.bmjjournals.com /cgi/content/full/42/3/214   (4381 words)

Somatic Cell-Like Features of Cloned Mouse Embryos Prepared with Cultured Myoblast Nuclei -- Gao et al. 69 (1): 48 -- ... Two kinds of myoblasts were employed in these studies. was significantly elevated in myoblast cloned embryos relative The myoblast cloned embryos at the late one-cell stage www.biolreprod.org /cgi/content/full/69/1/48   (6214 words)

FRG2, an FSHD candidate gene, is transcriptionally upregulated in differentiating primary myoblast cultures of FSHD ... FRG2, an FSHD candidate gene, is transcriptionally upregulated in differentiating primary myoblast cultures of FSHD patients -- Rijkers et al. Myoblasts were isolated from two healthy males and one healthy RNA was isolated during proliferation (prol) of primary myoblast cultures, and at three time points after the induction of differentiation by serum deprivation (day 2, 3, and 5). jmg.bmjjournals.com /cgi/content/full/41/11/826   (6709 words)

Autologous skeletal myoblast transplantation in patients with nonacute myocardial infarction: 1-year follow-up -- ... Autologous skeletal myoblast transplantation in patients with nonacute myocardial infarction: 1-year follow-up -- Gavira et al. Autologous skeletal myoblast transplantation in patients with nonacute myocardial infarction: 1-year follow-up skeletal myoblasts in conjunction with coronary artery bypass jtcs.ctsnetjournals.org /cgi/content/abstract/131/4/799   (398 words)

Stem Cell Network: Network Activities: Publications Myoblast survival enhancement and transplantation success improvement by heat shock treatment in mdx mice. P., and El Fahime, E. Growth factors improve the in vivo migration of human skeletal myoblasts by modulating their endogenous proteolytic activity. Nucleofection of muscle derived stem cells and myoblasts with C31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblasts. www.stemcellnet.ca /research/pubs.php   (8075 words)

Try your search on: Qwika (all wikis)

About us   |   Why use us?   |   Reviews   |   Press   |   Contact us   Copyright © 2005-2007 www.factbites.com Usage implies agreement with terms.