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Topic: Nuclear DNA

Related Topics

DNA replication

DNA sequences

mitochondrial DNA

DNA DNA hybridisation

DNA polymerases

DNA testing

junk DNA

DNA analysis

DNA motif

DNA fingerprinting

Noncoding DNA

DNA band

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	Mitochondrial DNA - Wikipedia, the free encyclopedia
		Unlike most of the cell, the function of which is defined by the nuclear DNA, the mitochondria have their own DNA and are assumed to have evolved separately.
		Human mitochondrial DNA consists of 5-10 rings of DNA and appears to carry 16,569 base pairs with 37 genes (13 proteins, 22 tRNAs and two rRNAs) which are concerned with the production of proteins involved in cellular respiration.
		The existence of mitochondrial DNA also supports the endosymbiotic theory, which suggests that eukaryotic cells first appeared when a prokaryotic cell was absorbed into another cell without being digested.
	en.wikipedia.org /wiki/Mitochondrial_DNA (736 words)

	Peterson (Site not responding. Last check: 2007-11-02)
		The interaction of nuclear DNA with oxidized polyphenols is inhibited by compounds that adsorb polyphenols or prevent oxidation reactions.
		The DNA can also be precipitated by making it 0.15 M sodium acetate, mixing the solution thoroughly with 2 volumes of ethanol, and spinning the resulting mixture in an ultracentrifuge at 32,000 rpm for 30 min at 4°C. The supernatants are decanted and the DNA pellets allowed to dry.
		Approximately 2-3 mg of DNA is obtained from 250 g of seedlings or 400 g of leaf tissue.
	www.uga.edu /ispmb/Pmbr15-2/Peterson.html (1782 words)

	DNA Virus Replication
		DNA replication is bidirectional (There are two replication forks per circular DNA genome and replication involves leading/lagging strands, Okazaki fragments, DNA ligase, etc.).
		This process of DNA replication is very similar to that which occurs in the host cell - which is not surprising as the virus is using mainly host machinery except for the involvement of the T antigen.
		VP1, 2 and 3 mRNAs are translated in the cytoplasm, the proteins are transported to nucleus, and capsids assemble with DNA (and cell histones) inside the capsid.
	pathmicro.med.sc.edu /mhunt/dna1.htm (3044 words)

	Galloanserae: A Critical Examination - EvoWiki (Site not responding. Last check: 2007-11-02)
		Given this conclusion, the discrepancy between the morphological data in the case of galloasnerine monophyly and that yielded by the sequencing of nuclear DNA, remains to be satisfactorily explained.
		Nuclear DNA sequencing is a far more reliable method that thus far does not appear to be significantly flawed methodologically, and thus its support for galloanserine monophyly remains paradoxical.
		Chubb, A. New nuclear evidence for the oldest divergence among neognath birds: the phylogenetic utility of ZENK (i).
	www.evowiki.org /index.php/Galloanserae:_A_Critical_Examination (5030 words)

	NOVA Online \| Cracking the Code of Life \| Journey into DNA (non-Flash)
		Although the DNA code from cell to cell is the same, there are many different types of cells within the body, each with a specific function.
		Note also that DNA with a "left-handed" twist, as this has, is a special kind of DNA known as Z-DNA.
		In the DNA molecule, A always pairs up with T, and C always pairs up with G. The sides of the DNA ladder consist of a long string of sugar and phosphate molecules, to which the bases are attached.
	www.pbs.org /wgbh/nova/genome/dna_sans.html (946 words)

	DNA Forensics
		Nuclear DNA must be extracted from samples for use in RFLP, PCR, and STR; however, mtDNA analysis uses DNA extracted from another cellular organelle called a mitochondrion.
		Her estranged husband was implicated because a snowy white cat hair was found in a jacket near the scene of the crime, and DNA fragments from the hair matched DNA fragments from Snowball, the cat belonging to the husband's parents.
		DNA can provide insights into many intimate aspects of a person and their families including susceptibility to particular diseases, legitimacy of birth, and perhaps predispositions to certain behaviors and sexual orientation.
	www.ornl.gov /sci/techresources/Human_Genome/elsi/forensics.shtml (3375 words)

	NOVA \| Last Flight of Bomber 31 \| Identifying Remains with DNA \| PBS
		One approach analyzes nuclear DNA and is useful in identifying someone who is alive or has died only recently.
		Unlike nuclear DNA, which is a mixture of genetic material from both parents, mtDNA is passed on, with no change, from mother to offspring.
		Whenever an egg cell is fertilized, nuclear chromosomes from a sperm cell enter the egg and, together with the egg's nuclear DNA, form the DNA for the new individual.
	www.pbs.org /wgbh/nova/bomber/mtdna.html (599 words)

	DNA glossary
		DNA, which consists of a polysugar- phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
		DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
		Nuclear DNA in eukaryotes (organisms with nucleated cells) is arranged into separate chromosomes and contains the bulk of the coding information.
	www.genomicglossaries.com /content/DNA.asp (2852 words)

	A PLANT NUCLEAR DNA PREPARATION PROCEDURE
		Rose du Ciel nuclear DNA suitable for restriction enzyme analysis and cloning.
		Greater chloroplast DNA contamination results when this method is used with the "Mitchell" line of Petunia.
		The mixture is heated at 60 C for 5 min and incubated with gentle shaking at 37 C for 5-10 hrs.
	wheat.pw.usda.gov /homepage/lazo/methods/lazo/dnaplpmb.html (718 words)

	Mitochondrial DNA (mtDNA) (Site not responding. Last check: 2007-11-02)
		There are two kinds of DNA that are found in a cell: nuclear and mitochondrial.
		Nuclear DNA is found within the nucleus of the cell and is composed of two sources of DNA.
		The CILHI uses this form of DNA because it preserves well in bones and many of the casualties that we are attempting to identify do not have blood samples on file (unlike the modern military).
	www.jpac.pacom.mil /mtDNA.htm (298 words)

	A New Nuclear Infrastructure
		Options proposed were transferring DNA's functions to individual services and the Advanced Research Projects Agency; maintaining DNA as a separate agency but tailoring it to today's national security environment; transferring functions to Department of Energy weapon laboratories; combining any of these options; or reorganizing DNA to reduce its costs significantly.
		Early in the study, it became clear that all the options for accomplishing DNA's functions had to be assessed in the broader context of the emerging national security environment and the overall nuclear infrastructure.
		DNA is only a small part of the nation's nuclear infrastructure (less than 10 percent in budgetary terms).
	www.rand.org /publications/RB/RB7103/RB7103.word.html (1121 words)

	Bending of Adenovirus Origin DNA by Nuclear Factor I as Shown by Scanning Force Microscopy Is Required for Optimal DNA ...
		A 711-bp DNA fragment was used containing 103 bp of the Ad5 origin of replication with the middle of the NFI consensus sequence at position 249 of the fragment.
		DNA and a clear increase of the bend angle was observed (Fig.
		DNA binding of NFI is not significantly affected by the mutations.
	jvi.asm.org /cgi/content/full/78/4/1928 (5817 words)

	A Nuclear Protein Involved in Apoptotic-like DNA Degradation in Stylonychia: Implications for Similar Mechanisms in ...
		Nuclear proteins with the same concentrations were separated by SDS-PAGE, stained with Coomassie Blue (a, c, e), blotted, and incubated with the Pdd1p antiserum (b, d, f).
		Macronuclear anlagen during polytene chromosome stage (a, b), at the beginning of DNA fragmentation (c-f), and in the vesicle stage during DNA degradation (g, h) were fixed, squashed, and stained with the Pdd1p antiserum (Cy2-stained anti-rabbit secondary antibody).
		Nuclear differentiation in ciliates is accompanied by extensive DNA rearrangement and elimination processes.
	www.molbiolcell.org /cgi/content/full/10/9/3003 (5437 words)

	BBC NEWS \| Science/Nature \| 'Better' DNA out of fossil bones (Site not responding. Last check: 2007-11-02)
		Many researchers would dearly love to get their hands on DNA samples from hominids further back in time - from those that lived 100,000 years ago or more - to find out how they were related to people alive today.
		So when we're dealing with a sample and you find it's got human DNA in it - is that DNA from the fossil, or is it actually DNA from the person who unearthed it?" says Professor Chris Stringer, the head of human origins at the Natural History Museum in London, UK.
		Studying the DNA not of modern humans but of their distant ancestors could help answer such questions.
	news.bbc.co.uk /2/hi/science/nature/4260334.stm (1184 words)

	News & Features: Evolutionary integration of mitochondrial DNA fragments into nuclear DNA is mutagenic
		Integration of mitochondrial DNA fragments into nuclear chromosomes (giving rise to nuclear DNA sequences of mitochondrial origin, or NUMTs) is an ongoing process that shapes nuclear genomes.
		This is indeed the case for novel insertions associated with human diseases and those driven by environmental insults.
		We thus propose a mutagenic phenomenon that may be responsible for a variety of genetic diseases in humans and suggest that genetic or environmental factors that increase the frequency of chromosome breaks provide the impetus for the continued colonization of the human genome by mitochondrial DNA.
	www.iscid.org /boards/ubb-get_topic-f-1-t-000170.html (521 words)

	Increased nuclear DNA content in developing cotton fiber cells -- Van't Hof 86 (6): 776 -- American Journal of Botany
		The amount of nuclear DNA at 2 dpa is 5.4 ± 0.27 pg.
		DNA and adds 1.3–1.4 pg of DNA between 3 and 5 dpa.
		Increased nuclear DNA content is independent of fertilization.
	www.amjbot.org /cgi/content/full/86/6/776 (2957 words)

	Molecular Cytogenetics Publications - Pat Heslop-Harrison & Trude
		Repetitive DNA sequences in Crocus vernus Hill (Iridaceae): the genomic organization and distribution of dispersed elements in the genus Crocus and its allies.
		Stability of transgenes and presence of N6 methyladenine DNA in transformed wheat cells.
		DNA density in mitotic and meiotic metaphase chromosomes of plants and animals.
	www.le.ac.uk /biology/phh4/titles.htm (4263 words)

	DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an ...
		DNA was from calf thymus and used at 0.05 mg/ml.
		DNA is shown by DAPI stainings in (Ac, f and i) and (Bb, e and i).
		Zhang,S., Maacke,H. and Grosse,F. (1995) Molecular cloning of the gene encoding nuclear DNA helicase II.
	nar.oxfordjournals.org /cgi/content/full/32/1/1 (5188 words)

	Damage to nuclear DNA induced by Shiga toxin 1 and ricin in human endothelial cells -- BRIGOTTI et al. 16 (3): 365 -- ...
		Damage to nuclear DNA induced by Shiga toxin 1 and ricin in human endothelial cells -- BRIGOTTI et al.
		Changes of nuclear morphology examined by Hoechst 33342 staining and fluorescence microscopy in HUVEC treated with 0.01 nM Stx1 or 1 nM ricin for 6 h or 24 h.
		DNA retained in the filter is lower vs. DNA eluted at pH 12.1.
	www.fasebj.org /cgi/content/full/16/3/365 (5431 words)

	Nuclear dna content of thirteen turfgrass species by flow cytometry -- Arumuganathan et al. 39 (5): 1518 -- Crop Science
		Nuclear dna content of thirteen turfgrass species by flow cytometry -- Arumuganathan et al.
		Nuclear dna content of thirteen turfgrass species by flow cytometry
		Arumuganathan K., Earle E.D. Estimation of nuclear DNA content of plants by flow cytometry.
	crop.scijournals.org /cgi/content/full/39/5/1518 (2064 words)

	Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A) -- Zhang et al. 112 (16): 2693 -- Journal of ...
		Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A) -- Zhang et al.
		Nuclear DNA helicase II (NDH II) is a highly conserved member of the DEXH
		Nuclear DNA Helicase II (RNA Helicase A) Interacts with Werner Syndrome Helicase and Stimulates Its Exonuclease Activity
	jcs.biologists.org /cgi/content/abstract/112/16/2693 (603 words)

	Estrogen Regulates the Association of Intermediate Filament Proteins with Nuclear DNA in Human Breast Cancer Cells -- ...
		Estrogen Regulates the Association of Intermediate Filament Proteins with Nuclear DNA in Human Breast Cancer Cells -- Spencer et al.
		Estrogen Regulates the Association of Intermediate Filament Proteins with Nuclear DNA in Human Breast Cancer Cells
		DNA or associated with the cytoskeleton of T-47D5 human breast
	www.jbc.org /cgi/content/abstract/273/44/29093 (460 words)

	An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo -- Jenke et al. 3 (4): 349 -- EMBO ... (Site not responding. Last check: 2007-11-02)
		Lane 1, total nuclear proteins from untransfected CHO cells; lane 2, proteins isolated from pEPI-1 transfected cells; lane 3, proteins isolated from pGFP-C1 transfected cells.
		Adachi, Y., Kas, E. and Laemmli, U.K. (1989) Preferential, cooperative binding of DNA topoisomerase II to scaffold-associated regions.
		Miskimins, W.K., Roberts, M.P., McClelland, A. and Ruddle, F.H. (1985) Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.
	emboreports.npgjournals.com /cgi/content/full/3/4/349 (3687 words)

	Distinct nuclear organization, DNA methylation pattern and cytokinin distribution mark juvenile, juvenile-like and ...
		Distinct nuclear organization, DNA methylation pattern and cytokinin distribution mark juvenile, juvenile-like and adult vegetative apical meristems in peach (Prunus persica (L.) Batsch) -- Bitonti et al.
		Role of DNA methylation in the regulation of gene expression in plants.
		Nuclear and genomic changes in floating and submerged buds and leaves of heterophyllous waterchestnut (Trapa natans L.).
	jxb.oxfordjournals.org /cgi/content/full/53/371/1047 (3674 words)

	NCFS: Biological Evidence
		The basic theory behind DNA testing is that no two individuals would be expected to posses the same so-called nuclear DNA type (except identical twins).
		It is now a matter of routine to obtain the DNA profile of the donor of a biological stain recovered at the crime scene.
		Mitochondrial DNA (mtDNA) analysis is often used in forensic cases in which traditional nuclear DNA is, or is likely to be, unsuccessful.
	ncfs.ucf.edu /bio_evd.html (3459 words)

	Nuclear DNA
		In current casework requiring the identification of human remains or other biological evidence, forensic scientists will use nuclear DNA typing methods to make an identification when other traditional forensic techniques, such as fingerprint and dental comparison cannot be used.
		In older cases, however, many different environmental factors, such exposure to the sun or heat, will destroy the nuclear DNA present in the biological material being studied.
		For most cases recovered from World War II, Korean War, Cold War, and Vietnam era losses, scientists normally use mitochondrial DNA sequencing to obtain the DNA results that can provide the additional evidence towards making an identification.
	www.dtic.mil /dpmo/family_support_info/nucleardna.htm (224 words)

	DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an ...
		DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner -- Zhang et al.
		An RNA-dependent association of Ku antigen with nuclear DNA
		was found in nuclear extracts of HeLa cells by immunoprecipitation
	nar.oxfordjournals.org /cgi/content/abstract/32/1/1 (452 words)

	[No title]
		Table I. Nuclear DNA content of a number of important plant species as determined by flow cytometry.
		Full Citation: Arumuganathan, K. and Earle, E.D. Nuclear DNA Content of Some Important Plant Species.
		This table has been reproduced in full with permission from the publisher.
	www.nal.usda.gov /pgdic/tables/nucdna.html (184 words)

	Upstate - Nuclear Function - DNA Replication
		• Immunoprecipitation Products - Nuclear Function IP Kits
		Click here to arrange these products by product type (antibodies, enzymes, etc.)
		Assay Reagents & Buffers - Nuclear Function Assay Reagents
	www.upstate.com /browse/subcategory.asp?subcategory=37 (56 words)

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