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Topic: Nucleoplasm

Related Topics

Chromatin

Chromosome

Nuclear membrane

Mitosis

Cell nucleus

Endoplasmic reticulum

Protoplasm

Cytoplasm

Eukaryote

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	Chromosome Motion and Mechanics Analyzed In Vivo
		The goal of this project is to develop a way to measure, quantitatively and noninvasively, the mechanical properties of chromosomes and the forces that act on them, inside a living cell.
		Specifically, we are using measurements of the three-dimensional movements of chromosomes during mitosis, to deduce the flexibility of the chromosomes, the viscosity of the nucleoplasm, and the magnitude of the forces acting on the kinetochore and the chromosome arms (the polar ejection force).
		Nucleoplasm viscosity is important becase it determines the velocity of motion produced by a given force acting on a chromosomes, and also may shed light on the physical structure of the nucleus.
	www.ucsf.edu /sedat/motion.html (862 words)

	Biology4Kids.com: Cell Structure: Cytoplasm
		The only two 'plasms' left are cytoplasm (the fluid in the cell also called cytosol) and nucleoplasm (the fluid in the nucleus).
		The nucleoplasm is the suspension fluid that holds the cell's chromatin and nucleolus.
		When the cell divides, the nuclear membrane dissolves and the nucleoplasm is released.
	www.biology4kids.com /files/cell_cytoplasm.html (312 words)

	Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3'-end formation and excision of the ...
		RNA was extracted from microdissected nucleoplasm and BR1 pre-mRNA was analyzed by RT-PCR.
		RNA was isolated from the microdissected BR1 gene locus (BR1) and nucleoplasm (NP) and the proportion of pre-mRNA with (unspliced) or without (spliced) intron 4, was determined.
		length of the poly(A) tail of the nucleoplasmic precursor.
	www.genesdev.org /cgi/content/full/12/17/2759 (6866 words)

	8.5.4.4
		The nucleoplasm is the semifluid matrix in the interior of the nucleus.
		An important task for early nanomedicine-oriented research will be to fully explore and elucidate this fine structure, and to determine whether or not it is stable enough to be relied upon in any way for intracellular navigation.
		Chromatin is composed of roughly equal amounts of negatively charged DNA (comprising the chromosomes) and globular histone proteins (basic proteins which carry a positive charge at the normal pH found in the cell).
	www.nanomedicine.com /NMI/8.5.4.4.htm (540 words)

	Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors ...
		Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm.
		In the nucleoplasm, Rev colocalized in a speckled pattern with
		RNA to spliced mRNA in the nucleoplasm of all cells.
	jvi.asm.org /cgi/content/abstract/68/3/1475 (396 words)

	New 'Biological' Robots Build Themselves \| LiveScience
		The interior of the nucleus is filled with a gel-like liquid known as nucleoplasm.
		Also present in the nucleoplasm are proteins known as polymerases, which pluck nucleotides from the soup as needed when copying DNA.
		The beauty of this approach is that the parts do not have to be presented in a specific order the way they are in a car assembly line.
	www.livescience.com /technology/050928_dna_robots.html (499 words)

	Stress-Dependent Nucleolin Mobilization Mediated by p53-Nucleolin Complex Formation -- Daniely et al. 22 (16): 6014 -- ...
		from the nucleolus to the nucleoplasm, whereupon it binds replication
		Lysates were analyzed for the presence of UBF (second panel), total p53 (third panel), and actin (used as a loading control) (bottom panel).
		The C terminus of p53, in combination with cell stress, is required for nucleolin mobilization.
	mcb.asm.org /cgi/content/full/22/16/6014 (5605 words)

	Reduced Mobility of the Alternate Splicing Factor (ASF) through the Nucleoplasm and Steady State Speckle Compartments ...
		Quantitative analysis of FRAP after spot-bleaching of a nuclear speckle and a nucleoplasmic region of the same cell.
		The relative concentration between the individual speckles and the surrounding nucleoplasm was normalized to one in the first image collected immediately after photobleaching.
		The ratio between fluorescence signal present within the speckles and the surrounding nucleoplasm is plotted over time as a mean of five separate speckles from individual experiments.
	www.jcb.org /cgi/content/full/150/1/41 (6673 words)

	Biological Robots Build Themselves
		The interior of the nucleus is filled with a gel-like liquid known as nucleoplasm.
		Also present in the nucleoplasm are proteins known as polymerases, which pluck nucleotides from the soup as needed when copying DNA.
		The beauty of this approach is that the parts do not have to be presented in a specific order the way they are in a car assembly line.
	www.roboticsdaily.com /headline/Biological-Robots-Build-Themselves.html (231 words)

	Science Fair Projects - Nucleoplasm
		Similar to the cytoplasm of a cell, the nucleus contains nucleoplasm.
		The nucleoplasm is a highly viscous solid containing the chromosomes and nucleoli.
		Chromosomes contain information encoded in DNA attached to proteins called histones and are usually arranged in to a dense network called chromatin.
	www.all-science-fair-projects.com /science_fair_projects_encyclopedia/Nucleoplasm (225 words)

	MPI.CBG research.
		Within nucleoplasm, the pre-mRNA substrates and the factors essential for the splicing are most highly concentrated at transcription sites (active genes), which are distributed apparently at random within the three-dimensional space of the nucleus.
		In addition to their nucleoplasmic distribution and function, some splicing factors are concentrated in CBs.
		The snRNP assembly factor SART3 shown in green is distributed throughout the nucleoplasm and in Cajal bodies.
	www.mpi-cbg.de /research/groups/neugebauer/neugebauer.html (600 words)

	Nucleoplasm - Encyclopedia, History, Geography and Biography
		It is enveloped by the nuclear membrane or nuclear envelope.
		The nucleoplasm is a highly viscous liquid that surrounds the chromosomes and nucleoli.
		Many substances such as nucleotides (necessary for purposes such as the replication of DNA) and enzymes (which direct activities that take place in the nucleus) are dissolved in the nucleoplasm.
	www.arikah.com /encyclopedia/Nucleoplasm (120 words)

	James A. Borowiec Ph.D.
		Stresses such as IR cause a dramatic redistribution of nucleolin from the nucleolus to the nucleoplasm.
		Under stress conditions, nucleolin relocalizes from the nucleolus to the nucleoplasm.
		In the nucleoplasm, nucleolin also binds to RPA and sequesters RPA from replication centers, contributing to the inhibition of chromosomal DNA replication.
	www.med.nyu.edu /Research/J.Borowiec-res.html (422 words)

	Nucleoplasm - Wikipedia, the free encyclopedia
		Similar to the cytoplasm of a cell, the nucleus contains nucleoplasm or nuclear sap.
		It is enveloped by the nuclear membrane or nuclear envelope.
		The nucleoplasm is a highly viscous liquid that surrounds the chromosomes and nucleoli.
	en.wikipedia.org /wiki/Nucleoplasm (143 words)

	nucleoplasm - OneLook Dictionary Search
		Tip: Click on the first link on a line below to go directly to a page where "nucleoplasm" is defined.
		nucleoplasm : The American Heritage® Dictionary of the English Language [home, info]
		Nucleoplasm : Drug Discovery and Development [home, info]
	www.onelook.com /?ls=a&w=nucleoplasm (178 words)

	Age-related changes in proliferating cell nuclear antigen levels.
		The nucleoplasm and cytoplasm from regenerating livers of 2-month and 24 month-old rats were fractionated by phosphocellulose column chromatography, aliquots of fractions were transferred to nitrocellulose filters and the amounts of PCNA in each fraction were measured by an immunostaining method.
		In control liver from aged rats, three types of PCNA in the cytoplasm and two types in the nucleoplasm were present at decreased levels.
		In regenerating liver from young rats, the increases in L type in the cytoplasm and H type in the nucleoplasm were remarkable.
	www.arclab.org /medlineupdates/abstract_9032755.html (347 words)

	Differential Subnuclear Localization of RNA Strands of Opposite Polarity Derived from an Autonomously Replicating ...
		The (-)-PSTVd (green) is localized in the nucleoplasm but not in the nucleolus, as indicated by the lack of overlap with U14 (red) in the merger image.
		DAPI stains the nucleoplasm but not the nucleolus, providing an additional marker for the location of the nucleolus.
		Fluorescence microscopic images of the spatial localization patterns of the (+)-strand of PSTVd that are suggestive of various stages of nucleolus-bound traffic of the RNA from the nucleoplasm.
	www.plantcell.org /cgi/content/full/15/11/2566/DC1?ck=nck (401 words)

	nucleoplasm
		nucleoplasm The non-staining or slightly chromophilic, liquid or semi-liquid, ground substance of the interphase nucleus and which fills the nuclear space around the chromosomes and the nucleoli.
		Little is known of the chemical composition of this ground substance, which is not easily defined.
		A high concentration of Ran-GTP defines the nucleoplasm relative to the cytoplasm, at least so far as the assembly and disassembly of nuclear transport...
	www.mongabay.com /igapo/biotech/nucleoplasm.html (89 words)

	Differential Subnuclear Localization of RNA Strands of Opposite Polarity Derived from an Autonomously Replicating ...
		in the nucleoplasm, and (3) the (+)-strand RNA is transported
		(2) the (-)-strand RNA is anchored in the nucleoplasm; (3) the
		The circular monomeric (+)-PSTVd traffics from the cytoplasm into the nucleoplasm to be used as the initial template to synthesize linear, multimeric (-)-PSTVd by DNA-dependent RNA polymerase II (Pol II).
	www.plantcell.org /cgi/content/full/15/11/2566 (6419 words)

	Remodelling of the nuclear lamina and nucleoskeleton is required for skeletal muscle differentiation in vitro -- ...
		Two hundred transfected cells were counted for strong staining of exogenous lamins in nucleoplasm (a) or at the nuclear envelope (b), or for expression of myogenin (c).
		nucleoplasm to the nuclear lamina is that, in postmitotic myoblasts,
		Remodelling of the nucleoskeleton by mutant lamin A is correlated
	jcs.biologists.org /cgi/content/full/118/2/409 (6861 words)

	The Role Of The Nucleus
		Electron microscopes have shown that nucleus have a sort of cytoplasm, called nucleoplasm; which is enclosed in a nuclear envelope.
		The nuclear envelope has many small pores on it, so the nucleoplasm is in contact with the normal cytoplasm.
		Replication of a DNA molecule involves taking nucleotides from the surrounding nucleoplasm and joining them together in exactly the same order, as they appear in the DNA molecule which is to be copied.
	library.thinkquest.org /22016/contribute/nucleus.htm (1254 words)

	BIOL414/614 at UMBC - ADAR_Location_Nucleolus
		It is suspected that the duplex regions of rRNA serve as substrates for ADAR2.
		Bleached cell nuclei are able to recover their fluorescence because EGFP-ADAR2 fused proteins enter the nucleolus from the nucleoplasm (Sansam et al 2003).
		Whereas shuttling of (i)ADAR1 across the nuclear membrane relies on the recognition of nuclear export signals by the nuclear pore complex, it is the dsRNA binding motifs (DRBMs) of ADAR2 that are responsible for its localization, via their ability to bind with rRNA in the nucleolus.
	www.umbc.edu /bioclass/biol414/wiki/index.php?page=ADAR_Location_Nucleolus (285 words)

	NUCLEAR AND CYTOPLASMIC VISCOSITY (Site not responding. Last check: )
		Introduction: The membrane of the nucleus is perforated by nuclear pores that permit the entry of water soluble molecules from the cytoplasm into the nucleoplasm.
		We can experimentally determine the viscosity of the cytoplasm and nucleoplasm by looking at the diffusion of molecules in the cytoplasm and nucleoplasm and relating this to a simple equation describing diffusion.
		Additionally, the slope of the nucleoplasm slightly steeper than that of the cytoplasm.
	www.tiem.utk.edu /~gross/bioed/webmodules/nuclearviscosity.htm (438 words)

	Kinetics of HCMV immediate early mRNA expression in stably transfected fibroblasts -- Snaar et al. 115 (2): 321 -- ...
		These images demonstrate the decrease in the amount of transcripts in the nuclear foci and in the nucleoplasm at different intervals after addition of a RNA polymerase II inhibitor.
		The amount of HCMV IE mRNAs in the remainder of the nucleoplasm increased at a slower rate after DRB removal.
		After induction of the HCMV IE gene expression by cycloheximide at 37°C, transcripts were observed in nuclear foci and in the remainder of the nucleoplasm.
	jcs.biologists.org /cgi/content/figsonly/115/2/321 (638 words)

	Phosphorylation-Dependent Nucleocytoplasmic Shuttling of Pancreatic Duodenal Homeobox-1 -- Elrick and Docherty 50 (10): ... (Site not responding. Last check: )
		of PDX-1 to the nucleoplasm was inhibited by wortmannin and
		The effect of glucose and insulin on the movement of PDX-1 from the nuclear periphery to the nucleoplasm is reversible.
		The effect of kinase inhibitors on the glucose- and insulin-stimulated movement of PDX-1 from the nuclear periphery to nucleoplasm.
	diabetes.diabetesjournals.org /cgi/content/full/50/10/2244 (4723 words)

	The nuclear envelope lamina network has elasticity and a compressibility limit suggestive of a molecular shock absorber ...
		The nuclear envelope, with attached lamina, detaches from the constant-size nucleoplasm during swelling.
		The nucleoplasm was highly deformable in unswollen nuclei (Fig.
		The nucleoplasm of the swollen nucleus remains external to the micropipette.
	jcs.biologists.org /cgi/content/full/117/20/4779 (4861 words)

	Amazon.com: nucleoplasm (Site not responding. Last check: )
		the biochemical processes in the nucleoplasm and a nebulous sense of...
		Cytoplasm and Nucleoplasm Except for the nucleus (or...
		RNA polymerase III in the nucleoplasm rather than in the nucleolus.
	www.amazon.com /s?ie=UTF8&keywords=nucleoplasm&tag=lexico&index=blended&link_code=qs&page=1 (1130 words)

	Chapter Novennial <i>to</i> Nucleus of N by Webster's Dictionary (1913 Edition)
		The matter composing the nucleus of a cell; the protoplasm of the nucleus; karyoplasma.
		Of or pertaining to nucleoplasm; — esp. applied to a body formed in the developing ovum from the plasma of the nucleus of the germinal vesicle.
		A kernel; hence, a central mass or point about which matter is gathered, or to which accretion is made; the central or material portion; — used both literally and figuratively.
	www.bibliomania.com /2/3/257/1205/23456/5.html (299 words)

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