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The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA : Article : Nature Although we were unable to isolate nucleoprotein in a single molecular form, nucleoprotein samples collected from the oligomeric peak fractions of a gel filtration column produced thin-plate crystals that diffract to 3.0 Å at synchrotron radiation sources. The nucleoprotein crystal structure shows that a deep groove between the head and body domain, which is at the exterior of nucleoprotein oligomers, is probably the RNA-binding site (Figs 3b, d and 5). At a nucleoprotein:RNA ratio of 1:1 and 2:1, 95% and 100% of the RNA, respectively, is bound to nucleoprotein (Supplementary Fig. npg.nature.com /nature/journal/v444/n7122/full/nature05379.html   (3627 words)

A Novel Function of Rad54 Protein. STABILIZATION OF THE Rad51 NUCLEOPROTEIN FILAMENT -- Mazin et al. 278 (16): 14029 -- ... Nucleoprotein complexes were separated from unbound proteins by centrifugation, and proteins bound and those remaining free were analyzed by SDS-polyacrylamide gel electrophoresis (the image of the gel is simply an illustration of the expected results). The stability of the nucleoprotein complexes was challenged by NaCl for 5 min at 37 °C. The amounts of Rad51 protein, Rad54 protein, and K341R Rad54 protein bound to ssDNA and dsDNA were determined using SDS-polyacrylamide gel electrophoresis. The stability of the nucleoprotein complex was challenged by NaCl for 5 min at 37 °C. For each Rad54 protein concentration, the STMP was determined from experiments done at 5-11 different NaCl concentrations. www.jbc.org /cgi/content/full/278/16/14029   (6214 words)

Virology Journal | Full text | Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells ... Nucleoprotein 1 (N1) encoded from the first open reading frame (ORF) was predicted to be 394 amino acids in length for aMPV/C/US/Co and 391 amino acids in length for aMPV/A/UK/3b with approximate molecular weights of 43.3 kilodaltons and 42.7 kilodaltons, respectively. Nucleoprotein 2 (N2) was hypothesized to be encoded by a second downstream ORF in-frame with ORF1 and encoded a protein predicted to contain 328 amino acids for aMPV/C/US/Co or 259 amino acids for aMPV/A/UK/3b with approximate molecular weights of 36 kilodaltons and 28.3 kilodaltons, respectively. The aMPV/C/US/Co nucleoprotein is encoded by the N gene with a predicted molecular weight of 42–45 kD [7,19]. www.virologyj.com /content/2/1/31   (4485 words)

A novel function of Rad54 protein - Stabilization of the Rad51 nucleoprotein filament nucleoprotein filament and stimulates its DNA pairing activity, suggesting that Rad54 protein is a component of the nucleoprotein complex involved in the DNA homology search. The Rad54-stabilized nucleoprotein filament is more competent in DNA strand exchange and acts over a broader range of solution conditions. nucleoprotein filament represents a novel means of stabilizing the biochemical entity central to homologous recombination, and reveals a new function of Rad54 protein. repositories.cdlib.org /postprints/1311   (251 words)

Overview Page Our goal is to develop technologies to visualize the assembly, function, and disassembly of individual nucleoprotein machines in their natural milieu. To transcend this limitation, it is essential to be able to observe and analyze single nucleoprotein machines. We hope to identify common principles that can be applied to the design of artificial nucleoprotein machines with novel specificities, facilitating the precise manipulation of DNA and RNA at the atomic level. www.nucleoproteinmachines.org /overview.htm   (919 words)

TSRI - News and Publications Subsequently, a chronic CNS disease developed that was characterized by infiltration of autoreactive T cells, microgliosis, and upregulation of MHC class I and class II molecules on microglia, oligodendrocytes, and endothelial cells. In order to investigate the effects of CNS expression of costimulatory proteins, transgenic mice that expressed the LCMV nucleoprotein in oligodendrocytes were bred to other transgenic mice that expressed the costimulatory protein B7-1 in oligodendrocytes. However, when the mice were infected with LCMV, the kinetics and severity of the resulting CNS disease were greater in mice expressing both the LCMV nucleoprotein and the B7-1 transgenes than in mice expressing only the viral transgene. www.scripps.edu /news/sr/sr98/npgen17.htm   (292 words)

A novel property of the RecA nucleoprotein filament: activation of double- stranded DNA for strand exchange in trans -- ... A RecA nucleoprotein filament is assembled on ssDNA in the presence of ATP with the RecA nucleoprotein filament, presumably, occupying the The RecA nucleoprotein filament was formed as for DNA strand exchange in trans, except that the concentration of DTT in the www.genesdev.org /cgi/content/full/13/15/2005   (6139 words)

The Scientist : Influenza nucleoprotein structure deciphered Researchers have unraveled the structure of the influenza A virus nucleoprotein, a development that might lead to the functional design of protein inhibitors used in antiviral therapeutics, according to a report in Nature. The viral nucleoprotein has two well known functions: It provides the scaffold for the ribonucleoprotein double helix, which is the complex in which viral RNA is packaged, and it also regulates viral RNA replication through interaction with the viral RNA polymerase. One implication of this structure is that the RNA binds to the outside in the ribonucleoprotein complex. www.the-scientist.com /news/home/37353   (654 words)

Nanomedicine Center for Nucleoprotein Machines Nucleoprotein machines carry out synthesis, modification, and repair of DNA and RNA. Our nanomedicine development center focuses on a model nucleoprotein machine that carries out non-homologous end joining (NHEJ) of DNA double strand breaks. This Center is part of the US National Institutes of Health Roadmap Initiative. www.nucleoproteinmachines.org   (217 words)

Expression of the Nucleoprotein of the Puumala Virus from the Recombinant Semliki Forest Virus Replicon: ... Expression of the Nucleoprotein of the Puumala Virus from the Recombinant Semliki Forest Virus Replicon: Characterization and Use as a Potential Diagnostic Tool -- Billecocq et al. The nucleoprotein gene was synthesized by RT-PCR using the RNA that with the native antigen (256 with recombinant nucleoprotein cdli.asm.org /cgi/content/full/10/4/658   (4122 words)

In silico analysis of genes nucleoprotein, neuraminidase and hemagglutinin: A comparative study on different strains of ... The proteins, nucleoprotein (NP), neuraminidase (NA) and hemagglutinin (HA) of influenza A virus (Bird flu virus) sub-type A/Hatay/2004/(H5N1) from chicken were selected for this study. The genes for nucleoprotein, neuraminidase and hemagglutinin of influenza A virus (bird flu virus) sub-type A/Hatay/2004/(H5N1) from chicken were analyzed. Arrese, M. and Portela, A. Serine 3 Is critical for phosphorylation at the N-terminal end of the nucleoprotein of influenza virus A/Victoria/3/75. www.bioinfo.de /isb/2006/06/0015/main.html   (2822 words)

Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. RT is recruited in these nucleoprotein complexes and synthesizes minus-strand cDNA initiated at the PBS. We also show that Ty3 nucleoprotein complexes were formed in vitro and that the N terminus of NCp9, but not the zinc finger, is required for complex formation, tRNA annealing to the PBS, RNA dimerization, and primer tRNA-directed cDNA synthesis by Ty3 RT. www.aegis.com /aidsline/2000/may/A0051976.html   (558 words)

[Frontiers in Bioscience 3, d570-603, June 17, 1998] Although the three proteins do form structurally similar nucleoprotein filaments (figure 5), and they do have significant homology at the amino acid level, they are significantly different in key regions, making each protein unique. Within the nucleoprotein filament, the conformation of both ss- and dsDNA is significantly different from that of native B-form DNA. Although Rad51 protein is able to form filaments on duplex DNA, this nucleoprotein filament is not active for DNA strand exchange (63). www.bioscience.org /1998/v3/d/bianco/5.htm   (4608 words)

The N Terminus of Rift Valley Fever Virus Nucleoprotein Is Essential for Dimerization -- Le May et al. 79 (18): 11974 ... The N Terminus of Rift Valley Fever Virus Nucleoprotein Is Essential for Dimerization -- Le May et al. The N Terminus of Rift Valley Fever Virus Nucleoprotein Is Essential for Dimerization The conserved residues at the N termini of phlebovirus N proteins are essential for N-N interactions. jvi.asm.org /cgi/content/full/79/18/11974   (4131 words)

Full restoration of viral fitness by multiple compensatory co-mutations in the nucleoprotein of influenza A virus ... Sequence variation in a newly identified HLA-B35-restricted epitope in the influenza A virus nucleoprotein associated with escape from cytotoxic T lymphocytes. Portela, A. and Digard, P. The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. Sequence variation in the influenza A virus nucleoprotein associated with escape from cytotoxic T lymphocytes. vir.sgmjournals.org /cgi/content/full/86/6/1801   (2710 words)

Medical Dictionary: Nucleoprotein - WrongDiagnosis.com Nucleoprotein : any supramolecular complex of protein and nucleic acid (DNP, RNP); or, any protein usually found closely associated with nucleic acid (e.g., histones). Other terms that may be related to Nucleoprotein: The description of Nucleoprotein may also be used for the following terms: www.wrongdiagnosis.com /medical/nucleoprotein.htm   (191 words)

Chapter2: THE NORMAL AND ADAPTED CELL Of particular importance are the nucleoproteins which are found in both the nucleus and cytoplasm of the cell. The nucleoproteins of the nucleus contain deoxyribonucleic acid (DNA) which constitutes the genes. Thus, the genes control the overall function of the cell as well as the transmission of hereditary characteristics from cell to cell. compepid.tuskegee.edu /syllabi/pathobiology/pathology/genpath/chapter2.html   (6865 words)

Curriculum Vitae: Professor Garth L. Nicolson   (Site not responding. Last check: 2007-10-26) "Nucleoproteins and their relationship to cytokines, gene, and HIV tracking." Invited lecture at University of Tennessee, Memphis, Tennessee, May 3, 1994. "Nucleoproteins, cytokines, gene and HIV tracking: A novel perspective." Invited lecture at Department of Laboratory Medicine, The University of Texas M. Anderson Cancer Center, Houston, Texas, September 16, 1994. Using a technique that involves direct restriction digestion of the nucleolus or nucleus followed by gentle acidic extraction and low ionic strength electrophoresis, NP complexes have been identified that are capable of discrete and faithful transcription as well as DNA synthesis using endogenous rather than synthetic oligonucleotide substrates. www.immed.org /cv/nancy_nicholson_cv.html   (4134 words)

The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication -- Portela and ... Davey, J., Dimmock, N. and Colman, A. Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes. Lin, B.-C. and Lai, C.-J. The influenza virus nucleoprotein synthesized from cloned DNA in a simian virus 40 vector is detected in the nucleus. Mandler, J., Müller, K. and Scholtissek, C. Mutants and revertants of an avian influenza A virus with temperature-sensitive defects in the nucleoprotein and PB2. vir.sgmjournals.org /cgi/content/full/83/4/723   (7034 words)

Production, Characterization, and Uses of Monoclonal Antibodies against Recombinant Nucleoprotein of Elk Coronavirus -- ... Cloning of the nucleoprotein gene of ECV in prokaryotic expression vector. The ECV nucleoprotein gene was subcloned in the pQE-30 expression vector, and the recombinant N protein with a polyhistidine Plates b, d, and f are immunostained with monoclonal antibody 8F2, which recognizes the nucleoprotein. cdli.asm.org /cgi/content/full/6/3/341   (2307 words)

Chemicon - Product #4050 - LIGHT DIAGNOSTICS™ Measles IgM Capture EIA Kit, ~48 tests [Rubeola Measles] The Light Diagnostics Measles Capture Enzyme Immunoassay utilizes a recombinant measles virus nucleoprotein antigen (8) and corresponding monoclonal antibodies in a solid phase IgM capture assay for the detection of measles specific serum IgM. Infected cells contain large amounts of nucleoprotein antigen which is the major internal protein of measles virus (6). Decontaminate with 0.05% sodium hypochlorite (a 1/100 dilution of household bleach) prior to disposal. www.chemicon.com /browse/productdetail.asp?ProductID=4050   (1209 words)

Detection of Canine Distemper Virus Nucleoprotein RNA by Reverse Transcription-PCR Using Serum, Whole Blood, and ... The oligonucleotides used for amplification of the CDV nucleoprotein (NP) gene sequences are shown in Fig. In vivo and in vitro detection of canine distemper virus nucleoprotein gene with digoxigenin-labelled RNA, double-stranded DNA-probes and oligonucleotides by in situ hybridization. Lack of correlation between virus nucleoprotein and mRNA expression and the inflammatory response in demyelinating distemper encephalitis indicates a biphasic disease process. jcm.asm.org /cgi/content/full/37/11/3634   (5092 words)

Characterization Of T-Cell Epitopes In Measles Virus NucleoProtein T-Cell Epitopes of the Measles Virus (MV) NucleoProtein were studied by synthesizing overlapping 20 aa Peptides over the known sequence of the protein and analyzing the proliferation responses of a panel of MV-specific T-Cell lines and clones against these Peptides. The results suggest that only a few Epitopes of the MV NucleoProtein are important in establishing T-Cell Immunity. Permission is granted to MS Societies and all MSers to utilize information from these pages provided that no financial reward is gained and attribution is given to the author/s. members.tripod.com /thjuland/measles-tcell.html   (429 words)

Cytosolic Delivery of Viral Nucleoprotein by Listeriolysin O-Liposome Induces Enhanced Specific Cytotoxic T Lymphocyte ... In this study, we have investigated the potential ability of listeriolysin O-containing liposomes (LLO-liposomes) to deliver lymphocytic choriomeningitis virus (LCMV) nucleoprotein (NP), harnessing the intracellular invasion mechanism of Listeria monocytogenes, to stimulate a NP-specific CTL response. Induction of virus-specific CTL is critical both to the control of LCMV infection and to the effective protective immunity. The results show that, while the humoral responses were induced to similar extents, the CTL response to viral nucleoprotein was significantly augmented by the inclusion of LLO in the liposomes. pubs.acs.org /cgi-bin/sample.cgi/mpohbp/2004/1/i01/html/mp034021m.html   (4233 words)

Influenza’s long tail The protein tail is present in common human influenza A which kills thousands of people every year as well as rare forms such as bird flu. US scientists used crystallography to study the long flexible tail of the influenza virus’ nucleoprotein. They found that even seemingly insignificant changes to the structure of this protein tail prevent it from fulfilling a key role in viral replication. www.sciencebase.com /science-blog/influenzas-long-tail.html   (239 words)

A Cis-spreading Nucleoprotein Filament Is Responsible for the Gene Silencing Activity Found in the Promoter Relay ... A Cis-spreading Nucleoprotein Filament Is Responsible for the Gene Silencing Activity Found in the Promoter Relay Mechanism -- Chen et al. A cis-spreading nucleoprotein filament is responsible for gene silencer AT8-mediated transcriptional repression. The quantified promoter activity shown at the bottom of each lane for three repeated experiments is expressed as the mean within the range of ±0.02 (S.D.). www.jbc.org /cgi/content/full/280/6/5101   (7182 words)

Mumps nucleoprotein antibody [8H4] (ab9392) datasheet   (Site not responding. Last check: 2007-10-26) Store at +4°C. At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mumps nucleoprotein antibody [8H4] is on this datasheet. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. If you publish research using ab9392 please let us know so that we can cite the reference on this datasheet. www.abcam.com /?datasheet=9392   (265 words)

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