
	Where results make sense

Topic: Oligomycin

In the News (Fri 1 Jan 10)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Metabolic poisons
		Oligomycin, an antibiotic, acts by binding ATP synthase in such a way as to block the proton channel.
		That is the mechanism by which oligomycin inhibits oxidative phosphorylation.
		Experimentally, oligomycin has no effect on state IV respiration, that is, it has no direct effect on electron transport or the chemiosmotic gradient.
	www.ruf.rice.edu /~bioslabs/studies/mitochondria/mitopoisons.html (1132 words)

	Mechanism Responsible for Oligomycin-induced Occlusion of Na+ within Na/K-ATPase -- Arato-Oshima et al. 271 (41): 25604 ...
		Oligomycin appeared to inhibit ouabain binding in a noncompetitive manner, and Na/K-ATPase isoforms with high and low sensitivities to ouabain were equally sensitive to oligomycin.
		in the absence of oligomycin and 1.65 nmol/mg and 7 and 4.3 µ
		Examination of the three-dimensional structure of oligomycin B showed that it has a protruding structure attached to a circular structure (43) and the protruding portion, the length and width of which are around 7Å and 2Å, respectively, appears favorable for interacting with the Na occlusion site of Na/K-ATPase embedded in the transmembrane segments.
	www.jbc.org /cgi/content/full/271/41/25604 (5617 words)

	Discovery provides reminder of bacteriology prof (Nov 4, 2003)
		The small oligomycin stash not only serves as a reminder of one of the Bacteriology Department's most esteemed scientists of the past, but might also provide a modest income to the department in the future.
		Oligomycin inhibits a key enzyme in the mitochondria of cells, making it useful for studies of cellular respiration, the process by which cells convert the energy stored in the sugar glucose to a form that can fuel a cell's chemical reactions.
		Oligomycin, like other macrolide antibiotics, is highly stable when stored dry at room temperature, says pharmacology professor Bernard Weisblum — just the conditions that existed in Fred Hall's basement safe.
	www.news.wisc.edu /9144.html (826 words)

	HGF promotes adhesion of ATP-depleted renal tubular epithelial cells in a MAPK-dependent manner -- Liu et al. 281 (1): ...
		and 25 nM oligomycin A for 30 min (3, 28, 50).
		oligomycin A and 2-deoxyglucose (90% ATP depletion = 9.3 ± 0.5%
		ATP levels were determined from mIMCD-3 cells at baseline or after incubation in the appropriate concentrations of oligomycin A and 2-deoxyglucose for 30 min followed by recovery in glucose-containing media for 30 or 60 min.
	ajprenal.physiology.org /cgi/content/full/281/1/F62 (5712 words)

	Excessive ATP hydrolysis in ischemic myocardium by mitochondrial F1F0-ATPase: effect of selective pharmacological ...
		Effect of 10 µM oligomycin given 2 min before the onset of ischemia on ATP concentrations in rat hearts before, during, and after global ischemia.
		Upon reperfusion, ATP began to recover in vehicle-treated hearts, and this effect was abolished by oligomycin.
		attenuated by oligomycin (0.19 ± 0.0.01 µmol ADP·min
	ajpheart.physiology.org /cgi/content/full/287/4/H1747 (5531 words)

	Perfluidone (37924-13-3). Adverse Effects. Fluoride Action Network Pesticide Project.
		Abstract: The influence of perfluidone (37924133) on oligomycin inhibited mitochondrial respiration was investigated in-vitro.
		Aliquots of 50 nanomolar adenosine-diphosphate, oligomycin, and perfluidone at concentrations from 0 to 160 millimolar (mM), or carbonylcyanide-p-trifluoromethoxyphenyl-hydrazone (FCCP), a classic protonophore, were added at specific time intervals.
		After adding FCCP at 1microM, the oligomycin inhibited respiratory rate of 31.4ng/mg/minute was increased to 151.3ng/mg/minute.
	www.fluoridealert.org /pesticides/epage.perfluidone.htm (488 words)

	Oligomycin sensitivity of mitochondrial F1F0-ATPase in diabetes-prone BHE/Cdb rats -- Kim and Berdanier 277 (4): E702 ...
		Oligomycin sensitivity of mitochondrial F1F0-ATPase in diabetes-prone BHE/Cdb rats -- Kim and Berdanier 277 (4): E702 -- AJP - Endocrinology and Metabolism
		OXPHOS with respect to the response to oligomycin.
		Oligomycin binding is usually thought to be the responsibility of the oligomycin-conferring protein located in the stalk of
	ajpendo.physiology.org /cgi/content/full/277/4/E702 (2763 words)

	Dynamic adaptation of cardiac oxidative phosphorylation is not mediated by simple feedback control -- van Beek et al. ...
		Assessment of inhibition of ATP synthetic capacity by oligomycin in isolated mitochondria.
		Myocardial respiration after oligomycin administration normalized to baseline value before oligomycin (left), and left ventricular systolic pressure after oligomycin administration normalized to baseline value (right) determined at 60 beats/min in the intact heart.
		by the inhibition by oligomycin of the ATP synthetic capacity.
	ajpheart.physiology.org /cgi/content/full/277/4/H1375 (5915 words)

	Contraction-Induced Fatty Acid Translocase/CD36 Translocation in Rat Cardiac Myocytes Is Mediated Through AMP-Activated ...
		oligomycin or 100 µmol/l dipyridamole for 15 min.
		Effects of AICAR and oligomycin in the absence and presence of electrical stimulation on substrate uptake by cardiac myocytes.
		Cell suspensions were incubated in the absence of additions (Ctlr) or presence of 1.0 mmol/l AICAR or 30 µmol/l oligomycin (Oli) for 15 min before execution of palmitate uptake studies (3 min; left panel) or deoxyglucose uptake studies (3 min; right panel) without and with electrical field stimulation at 200 V and 4 Hz (200V/4Hz).
	diabetes.diabetesjournals.org /cgi/content/full/52/7/1627 (6258 words)

	86C-4 Effects of dietary fat on mitochondrial respiration in the NIDDM-prone rat: Inhibition of respiration by ... (Site not responding. Last check: 2007-10-09)
		This study was attempted to answer the question whether altering the fatty acid composition of membrane profoundly affects the mitochondrial respiration in the NIDDM-prone rat.
		The responsiveness of hepatic mitochondria isolated from BHE/Cdb rat fed a 6% coconut oil or corn oil to oligomycin, a specific inhibitor of tightly coupled respiration, was performed.
		It is speculated that the fatty acid composition of the membrane could have had an effect on inhibition of respiration by oligomycin.
	ift.confex.com /ift/2000/techprogram/paper_3979.htm (339 words)

	Mitochondria-dependent apoptosis and cellular pH regulation (Site not responding. Last check: 2007-10-09)
		Oligomycin also inhibited cell death induced by p53, in a cell line where p53 is known to induce transcription of the endogenous bax gene and to trigger apoptosis through a bax-dependent mechanism.
		Moreover, oligomycin reportedly prevents the rise in membrane potential and matrix pH induced by mitochondria-dependent cell death stimuli such as Bax or staurosporine.
		Use of any pharmacological agent, including oligomycin, for dissecting cellular mechanisms is subject to caveats, and thus the results reported thus far should be viewed as preliminary.
	www.nature.com /cdd/journal/v7/n12/full/4400779a.html (8983 words)

	Understanding and exploiting the mechanistic basis for selectivity of polyketide inhibitors of F0F1-ATPase -- Salomon ...
		By comparison of the pattern of sensitivity of a molecule to the approximately 70,000 molecules previously examined, one can often discover molecules of known mechanism of action that show high correlation to the compound, suggesting a similar mechanism of action (7).
		The mean IC values for apoptolidin, cytovaricin, oligomycin A, and ossamycin were 18 µM, 1 µM, 0.27 µM, and 0.8 µM, respectively.
		Apoptolidin was isolated from the producing organism as described in ref. 2.
	www.pnas.org /cgi/content/full/97/26/14766 (2277 words)

	Down-regulation of Mitochondrial F1F0-ATP Synthase in Human Colon Cancer Cells with Induced 5-Fluorouracil Resistance ...
		A, cell proliferation is suppressed by the ATP synthase inhibitors, oligomycin A, and aurobertin B. After 4 days of incubation with an ATP synthase inhibitor, cell proliferation was determined by MTT assay.
		Cells were incubated with 0.1 µmol/L oligomycin in the presence or absence of 5-FU IC < 0.001 when compared with cell proliferation suppressed by 5-FU alone.
		Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis.
	cancerres.aacrjournals.org /cgi/content/full/65/8/3162 (4310 words)

	Permeability transition in rat liver mitochondria is modulated by the ATP-Mg/Pi carrier -- Hagen et al. 285 (2): 274 -- ...
		Oligomycin (1 µg/ml) or carboxyatractyloside (CA; 5 µM) was added as indicated.
		and in the absence or presence of 1 µg/ml oligomycin.
		Swelling was induced by the addition of 50 µM Ca The matrix adenine nucleotide contents given in the table were measured in the same mitochondrial preparations under identical incubation conditions.
	ajpgi.physiology.org /cgi/content/full/285/2/G274 (3451 words)

	Perfluidone (37924-13-3) Abstracts. Fluoride Action Network Pesticide Project.
		Sensitivity of oligomycin-inhibited respiration of isolated rat liver mitochondria to perfluidone, a fluorinated arylalkylsulfonamide.
		Oxygen electrode polarographic measurements of the rate of oxygen consumption by isolated rat liver mitochondria revealed that oligomycin inhibition of respiration was offset to different degrees by varying concentrations of perfluidone (1,1,1-trifluoro-N-(2 methyl-4-(phenylsulfonyl) methanesulfonamide).
		An initial inhibition of respiration by oligomycin is due to an interaction with the proton well of FOF1-ATP synthetase (Lardy, H.A. et al., Arch.
	www.fluoridealert.org /pesticides/perfluidone.abstracts.htm (1354 words)

	Evidence for Interactions between Intracellular Calcium Stores during Methylmercury-Induced Intracellular Calcium ...
		B, mitochondrial membrane depolarization abolished the initial elevation of rhod-2 fluorescence without affecting the time-to-onset of the spreading of rhod-2 throughout the cytosol.
		Treatment with CCCP and oligomycin abolished the gradual increase of rhod-2 fluorescence observed in MeHg treated cells, thus the first phase and second phase increase in fluorescence occurred simultaneously.
		increase that was abolished by CCCP and oligomycin, and a secondary
	jpet.aspetjournals.org /cgi/content/full/304/3/949 (5593 words)

	Prior Exposure to High Glucose Augments Depolarization-Induced Insulin Release by Mitigating the Decline of ATP Level ...
		The oligomycin used is a mixture of several oligomycins (A, B, and C).
		Effects of 2 µg/ml oligomycin on depolarization-induced insulin release from primed and nonprimed intact islets.
		Oligomycin was present only during the final incubation.
	endo.endojournals.org /cgi/content/full/143/1/213 (5399 words)

	Moye-Rowley Physiology Laboratory : Research Activities
		YOR1 encodes an ABC transporter protein that is required for normal resistance to the mitochondrial ATPase inhibitor oligomycin.
		Transformants were spotted onto medium containing a gradient of oligomycin (increasing as denoted by the bar of increasing width).
		The easily selectable oligomycin resistance phenotype provided by yor1 mutants will be exploited to use genetics to examine this issue.
	www.uiowa.edu /~physio/res-yor1.html (839 words)

	APStracts 6:0128E, 1999. (Site not responding. Last check: 2007-10-09)
		Oligomycin sensitivity of mitochondrial f1f0atpase in diabetes -prone bhe/cdb rats1.
		When the isolated F1FoATPase complex was studied and ATPase activity assessed, that which was isolated from the BHE/Cdb rats was less sensitive to oligomycin inhibition than that isolated from the Sprague Dawley rats.
		These mitochondria were more sensitive to oligomycin inhibition of OXPHOS than were mitochondria isolated from Sprague Dawley rats.
	www.uth.tmc.edu /apstracts/1999/endo/June/128e.html (187 words)

	Katzmann, David J - The yeast ABC transporter YOR1 confers resistance to oligomycin. (Site not responding. Last check: 2007-10-09)
		Katzmann, David J - The yeast ABC transporter YOR1 confers resistance to oligomycin.
		We have recently identified the ABC transporter YOR1 (yeast oligomycin resistance 1).
		Additionally, Yor1p confers resistance to a second cytotoxic agent that is structurally unrelated to oligomycin, venturicidin, raising the possibility that Yor1p is a multidrug transporter.
	www.yeastgenome.org /community/meetings/yeast96/f2355.html (258 words)

	Mitochondrial Fusion in Human Cells Is Efficient, Requires the Inner Membrane Potential, and Is Mediated by Mitofusins ...
		Cells have detached from the substrate after combined treatment with oligomycin and deoxyglucose, and none of these treatments provokes the apoptotic morphology induced by staurosporine.
		Differently labeled 143B cells (A) or HeLa cells (B) were coplated and fused in the presence of 10 µM cccp (A) or 2.5 µM oligomycin (B) and cells were fixed 4 h after cell fusion.
		After 36 h, cells were fixed under control conditions or after a 4-h treatment with cccp or oligomycin.
	www.molbiolcell.org /cgi/content/full/13/12/4343 (6540 words)

	Mitochondria Control AMPA/Kainate Receptor-Induced Cytoplasmic Calcium Deregulation in Rat Cerebellar Granule Cells -- ...
		The use of oligomycin and FCCP to probe mitochondrial function in KA-exposed cells.
		In traces B, D, and F, 5 µg/ml oligomycin was present from the beginning of the experiment.
		NMDA receptor-mediated DCD in granule cells is unaffected by oligomycin (Budd and Nicholls, 1996b
	www.jneurosci.org /cgi/content/full/21/6/1893 (5520 words)

	Stress-induced activation of the p53 tumor suppressor in leukemia cells and normal lymphocytes requires mitochondrial ...
		Normal PB T cells and ALL cells were incubated with oligomycin (10 µg/mL) and/or etoposide (30 µM) for 6 hours and assessed for MTMP and p53 levels.
		MOLT-3 cells were incubated with oligomycin (10 µg/mL) and/or with etoposide (1 µM) for 3 hours and assessed for NAO fluorescence.
		of oligomycin and NAC on the etoposide-induced p53 accumulation.
	www.bloodjournal.org /cgi/content/full/105/12/4767 (5952 words)

	New Insights into the Bioenergetics of Mitochondrial Disorders Using Intracellular ATP Reporters -- Gajewski et al. 14 ...
		cannot be attributed to the effect of oligomycin.
		Rescue of a deficiency in ATP synthesis by transfer of MTATP6, a mitochondrial DNA-encoded gene, to the nucleus.
		Manfredi, G., Gupta, N., Vazquez-Memije, M.E., Sadlock, J.E., Spinazzola, A., De Vivo, D.C., and Schon, E.A. Oligomycin induces a decrease in the cellular content of a pathogenic mutation in the human mitochondrial ATPase 6 gene.
	www.molbiolcell.org /cgi/content/full/14/9/3628 (4670 words)

	Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements -- Ramanathan et al. 102 ...
		Mean graph of the small-molecule sensitivities of the four cell lines to oligomycin, oxamate, and 2-deoxyglucose.
		The mean small-molecule sensitivities of oligomycin, oxamate, and 2-deoxyglucose are 0.5, 1.4, and 3.6 mM, respectively.
		The four cell lines were perturbed by using 2-deoxyglucose, oligomycin, oxamate, rapamycin, and wortmannin as described in Materials and Methods.
	www.pnas.org /cgi/content/full/102/17/5992 (4005 words)

	Insulin and Contraction Stimulate Exocytosis, but Increased AMP-activated Protein Kinase Activity Resulting from ...
		oligomycin (Omo) for 5 or 60 min, or after electrically stimulated contraction (Ctr) for 1 and 5 min.
		The internalization of the biotin-tagged GLUT4 was allowed to proceed, and then avidin was added to the cells at the indicated times to quench any tagged GLUT4 that remained at the cell surface.
		In C, the internalization of transferrin was determined in cardiomyocytes that were either maintained in the basal state (Ba) or treated with oligomycin (OmO).
	www.jbc.org /cgi/content/full/280/6/4070 (6035 words)

	Wadsworth-Mitochondrial Conference 1998 (Site not responding. Last check: 2007-10-09)
		We have optimized conditions for monitoring mmp by both epifluorescence and confocal imaging and for detecting whether individual mitochondria are net synthesizers or utilizers of ATP during NMDA receptor activation.
		DCD is not due to a failure of mitochondrial ATP synthesis since it is seen in cells maintained by glycolysis in the presence of oligomycin.
		While SO generation is enhanced during NMDA receptor activation, it is unclear whether this is sufficient to account for DCD since the combination of antimycin A and oligomycin (which enhances SO generation) is as protective as rotenone/oligomycin (which does not).
	www.wadsworth.org /albcon98/abstract/nicholls.htm (310 words)

	Mitochondrial Control of Acute Glutamate Excitotoxicity in Cultured Cerebellar Granule Cells -- Castilho et al. 18 ...
		Under energy-deficient conditions, when in the presence of rotenone minus oligomycin (D) or in the absence of glucose (E), glutamate-glycine produces a large transient, followed by immediate deregulation.
		Granule cells were incubated in the presence of 5 µg/ml oligomycin.
		Population ATP/ADP ratios decline in granule cells exposed to glutamate in the presence or absence of oligomycin (Budd and
	www.jneurosci.org /cgi/content/full/18/24/10277 (6311 words)

	Actively phosphorylating mitochondria are more resistant to lactic acidosis than inactive mitochondria -- Tonkonogi and ...
		This effect of acidosis was attenuated in the presence of oligomycin.
		and oligomycin were sufficient to completely inhibit adenine nucleotide
		Effect of cyclosporine A and oligomycin on non-specific permeability of the inner mitochondrial membrane.
	ajpcell.physiology.org /cgi/content/full/277/2/C288 (3253 words)

	JOSHI, Saroj
		This process is catalyzed by an enzyme complex named ATP synthase, consisting of a catalytic segment F1, a proton-translocating segment Fo, and an intervening segment, stalk, that brings about the coupling of F1 and Fo segments.
		As its name implies, OSCP renders the F1 enzyme sensitive to the antibiotic oligomycin, which results in the inhibition of the activity of F1.
		It is hoped that this kind of analysis will lead to a better understanding of the mechanism of ATP synthesis in normal and diseased tissue, which may eventually help in diagnosing and possibly curing neurodegenerative diseases that involve defects in mitochondria.
	www.bbri.org /faculty/joshi/JOSHI.html (610 words)

Try your search on: Qwika (all wikis)