| | Nikon MicroscopyU: Phase Contrast Microscopy - Introduction |
 | | In optical terminology, phase plates that alter the phase of surround light relative to diffracted light by 90 degrees (either positive or negative) are termed quarter wavelength plates because of their effect on the optical path difference. |
 | | For arguments in phase contrast microscopy, the role of the specimen in altering the optical path length (in effect, the relative phase shift) of waves passing through is of paramount importance. |
 | | In terms of optical path variations between the specimen and its surrounding medium, the portion of the incident light wavefront that traverses the specimen (D-wave), but does not pass through the surrounding medium (S-wave), is slightly retarded. |
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