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Topic: Replication origin

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DNA replication

Replication fork

Lagging strand

DNA polymerase

Restriction enzyme

Endonuclease

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	Origin of replication - Wikipedia, the free encyclopedia
		The origin of replication (also called the replication origin) is a unique DNA sequence at which DNA replication is initiated.
		The origin of replication binds a member of the pre-replication complex—a protein complex that binds, unwinds, and begins to copy DNA.
		Having many origins of replication helps to speed the duplication of their (usually) much larger store of genetic material.
	en.wikipedia.org /wiki/OriC (266 words)

	DNA Replication
		This mode of replication is described as semi-conservative: one-half of each new molecule of DNA is old; one-half new.
		Replication begins at some replication origins earlier in S phase than at others, but the process is completed for all by the end of S phase.
		This redundancy probably reflects the crucial importance of precise replication to the integrity of the genome.
	users.rcn.com /jkimball.ma.ultranet/BiologyPages/D/DNAReplication.html (692 words)

	Interference of the Simian Virus 40 Origin of Replication by the Cytomegalovirus Immediate Early Gene Enhancer: ...
		the degree of inhibition of DNA replication and the decrease of
		SV40 origin and the degree of inhibition of SV40 origin-based
		The ration of intensity of DNA bands representing cleavage at the SV40 origin to the total intensity of DNA bands from the cleavage of SV40 and CMV regulatory sequence is plotted against the replication efficiency of the plasmid relative to the parental plasmid pSV2cat.
	mcb.asm.org /cgi/content/full/20/11/4062 (7740 words)

	DpiA Binding to the Replication Origin of Escherichia coli Plasmids and Chromosomes Destabilizes Plasmid Inheritance ...
		All lanes have 0.6 ng of the pSC101 origin fragment.
		The sequence of the pSC101 origin is known (49), and the sequencing tracts are shown for orientation.
		Revised interpretation of the origin of the pSC101 plasmid.
	jb.asm.org /cgi/content/full/185/20/6025 (4643 words)

	Sequence Diversity and Functional Conservation of the Origin of Replication in Lactococcal Prolate Phages -- Rakonjac ...
		origins of plasmid replication in the absence of phage proteins.
		Sequence diversity of the origin of replication in prolate phage.
		pTRKL2 is a lactococcal plasmid with pAMß1 origin of replication (37).
	aem.asm.org /cgi/content/full/69/9/5104 (5525 words)

	origin of replication
		An origin of replication is a sequence of DNA at which replication is initiated on a chromosome, plasmid or virus.
		The origin of replication determines the vector copy number, which could typically be in the range of 25—50 copies/cell if the expression vector is derived from the low-copy-number plasmid pBR322, or between 150 and 200 copies/cell if derived from the high-copy-number plasmid pUC.
		The introduction of a new origin, in the form of a second plasmid from the same compatibility group, mimics the result of replication of the resident plasmid.
	www.ls.huji.ac.il /~expression/vector/origin.html (318 words)

	Sequence-independent DNA binding and replication initiation by the human origin recognition complex -- Vashee et al. 17 ...
		Bell, S.P. and Stillman, B. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
		Ladenburger, E.M., Keller, C., and Knippers, R. Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication.
		Lee, J.K., Moon, K.Y., Jiang, Y., and Hurwitz, J. The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein.
	www.genesdev.org /cgi/content/full/17/15/1894 (8100 words)

	[No title]
		This is because DNA viruses have a well-defined origin of replication and generally use the same proteins that cells use to replicate their DNA.
		Replication protein A (RPA) is a single strand DNA binding protein that stabilizes melted ssDNA.
		The distance from the ends of the cut DNA to the center of the replication 'bubble' was found to be constant at all times even though the bubble grew in size around this center as replication progressed, indicating that replication is bidirectional from a common origin located at the center of the bubble.
	web.uconn.edu /mcb201/lecture09.html (808 words)

	Assembly of the replication initiation complex on SV40 origin DNA -- Simmons et al. 32 (3): 1103 -- Nucleic Acids ...
		Purified T antigen was incubated with an origin DNA fragment in the presence of different amounts of purifed topo I as shown.
		Lee,S.H., Ishimi,Y., Kenny,M.K., Bullock,P., Dean,F.B. and Hurwitz,J. (1988) An inhibitor of the in vitro elongation reaction of simian virus 40 DNA replication is overcome by proliferating-cell nuclear antigen.
		Tanaka,T. and Nasmyth,K. (1998) Association of RPA with chromosomal replication origins requires an Mcm protein and is regulated by Rad53 and cyclin- and Dbf4-dependent kinases.
	nar.oxfordjournals.org /cgi/content/full/32/3/1103 (6674 words)

	Localization of proteins bound to a replication origin of human DNA along the cell cycle -- Abdurashidova et al. 22 ... (Site not responding. Last check: 2007-11-06)
		The data of the experiments reported in Figures 2 and 3 are reported for the four investigated proteins as the relative abundance of ori versus non-ori sequences in the DNA isolated after treatment with the appropriate antibody at different time points of the cell cycle.
		Giacca,M., Pelizon,C. and Falaschi,A. (1997) Mapping replication origin by quantifying relative abundance of nascent DNA strands using competitive polymerase chain reaction.
		Ladenburger,E.M., Keller,C. and Knippers,R. (2002) Identification of a binding region for human origin recognition complex protein 1 and 2 that coincides with an origin of DNA replication.
	embojournal.npgjournals.com /cgi/content/full/22/16/4294 (6620 words)

	ISOLATION & CHARACTERIZATION OF MAIZE MITOCHONDRIAL DNA REPLICATION ORIGIN(S) (Site not responding. Last check: 2007-11-06)
		A replication origin in the maize cmsT mt genome has been localized by 2-Dimensional (2D) gel electrophoretic analyses of in vitro and in vivo DNA replication products and by the BND-cellulose chromatography of replication intermediates generated in vivo.
		These studies show that an origin of replication is located within a 5.1Kbp BamH I fragment present between 6 and 11Kbp repeat regions (coordinates 185-195) in the maize cmsT mt genome.
		In vitro replication of cyanobacterial plasmids from Synechocystis PCC6803.
	www.nal.usda.gov /pgdic/pggrantinfo/1992/9159563.html (421 words)

	Identification of Persistent RNA-DNA Hybrid Structures within the Origin of Replication of Human Cytomegalovirus -- ...
		Lanes: 1, total-cell DNA from a cotransfection using the required replication genes along with pOri and probed with pOri SB-B; 2, total-cell DNA from a cotransfection using the required replication genes along with pOri, except that the plasmid encoding UL105 was omitted.
		Replication of Epstein-Barr virus oriLyt: lack of a deticated virally encoded origin-binding protein and dependence on Zta in cotransfection assays.
		Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator.
	jvi.asm.org /cgi/content/full/72/9/6997 (5819 words)

	A Critical DnaA Box Directs the Cooperative Binding of the Escherichia coli DnaA Protein to the Plasmid RK2 Replication ...
		mutations in the plasmid replication origin that scrambled or
		The four DnaA boxes present at the leftward end of the origin are arranged as inverted pairs directly upstream of the TrfA binding sites (iterons) and the four 13-mers in the A+T-rich region that serve as the site of helix destabilization.
		Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus
	www.jbc.org /cgi/content/full/274/25/17918 (4846 words)

	Multiple Orientation-Dependent, Synergistically Interacting, Similar Domains in the Ribosomal DNA Replication Origin of ...
		Yompakdee, C., Huberman, J. Enforcement of Late Replication Origin Firing by Clusters of Short G-rich DNA Sequences.
		Kong, D., DePamphilis, M. Site-Specific DNA Binding of the Schizosaccharomyces pombe Origin Recognition Complex Is Determined by the Orc4 Subunit.
		Gómez, M., Antequera, F. Organization of DNA replication origins in the fission yeast genome.
	mcb.asm.org /cgi/content/abstract/18/12/7294 (747 words)

	Replication
		DNA replication is initiated at a region on a chromosome called an origin of replication.
		An enzyme called T-antigen (helicase) binds to the origin and unwinds the DNA in both directions from the origin.
		As the DNA is unwound specific single stranded DNA binding proteins (Replication factor A) prevent the strands from reannealing.
	bioweb.uwlax.edu /GenWeb/Molecular/Theory/Replication/replication.htm (137 words)

	Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe -- ... (Site not responding. Last check: 2007-11-06)
		Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe -- Dubey et al.
		DNA replication origins in the Schizosaccharomyces pombe genome
		Identification of a Compound Origin of Replication at the HMR-E Locus in Saccharomyces cerevisiae
	embojournal.npgjournals.com /cgi/content/abstract/13/15/3638 (1165 words)

	Strand Symmetry Around the {beta}-Globin Origin of Replication in Primates -- Pilar Francino* and Ochman 17 (3): 416 -- ...
		and downstream of the ß-globin origin and contrasts the
		Replication error rates for G.dGTP, T.dGTP, and A.dGTP mispairs and evidence for differential proofreading by leading and lagging strand DNA replication complexes in human cells.
		Preferential DNA secondary structure mutagenesis in the lagging strand of replication in E.
	mbe.oxfordjournals.org /cgi/content/full/17/3/416 (3308 words)

	Initiation of polyoma virus origin-dependent DNA replication through STAT5 activation by human granulocyte-macrophage ...
		The replicated DpnI-resistant plasmid is indicated by the arrow.
		Initiation of Py DNA replication occurs when the chromatin structure around the origin opens for the binding of LTag.
		B (AML1/Cbfa2) to stimulate polyomavirus DNA replication is related to its affinity for the nuclear matrix.
	www.bloodjournal.org /cgi/content/full/97/5/1266 (6027 words)

	The B2 element of the Saccharomyces cerevisiaeARS1 origin of replication requires specific sequences to facilitate ...
		The B2 element of the Saccharomyces cerevisiaeARS1 origin of replication requires specific sequences to facilitate pre-RC formation -- Wilmes and Bell 99 (1): 101 -- Proceedings of the National Academy of Sciences
		The minimal requirements for a eukaryotic origin of replication are an initiator binding site and a region of helically unstable
		The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription
	www.pnas.org /cgi/content/abstract/99/1/101 (525 words)

	Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by ...
		Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP -- McGarry et al.
		Initiation of DNA replication in eukaryotes, archea, and eubacteria
		proteins and origin DNA to mediate assembly of replisomes.
	www.pnas.org /cgi/content/abstract/101/9/2811 (471 words)

	Human Mcm proteins at a replication origin during the G1 to S phase transition -- Schaarschmidt et al. 30 (19): 4176 -- ...
		Human Mcm proteins at a replication origin during the G1 to S phase transition -- Schaarschmidt et al.
		Human Mcm proteins at a replication origin during the G
		enriched for a DNA region that includes a replication origin.
	nar.oxfordjournals.org /cgi/content/abstract/30/19/4176 (533 words)

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