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Topic: Outer membrane


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In the News (Fri 1 Jan 10)

  
  TonB Interacts with Nonreceptor Proteins in the Outer Membrane of Escherichia coli -- Higgs et al. 184 (6): 1640 -- ...
density gradients and the cytoplasmic membrane and outer membrane
membranes that was absent from the lpp outer membranes.
of outer membrane receptors by blebbing of the outer membrane,
jb.asm.org /cgi/content/full/184/6/1640   (5587 words)

  
 Cell membrane - Wikipedia, the free encyclopedia
A cell membrane, plasma membrane or plasmalemma is a selectively permeable lipid bilayer coated by proteins which comprises the outer layer of a cell.
In animal cells the plasma membrane alone establishes a separation between interior and environment, whereas in fungi, bacteria, and plants an additional cell wall forms the outermost boundary.
The outer cell membrane and the membranes surrounding inner cell organelles are lipid bilayers.
en.wikipedia.org /wiki/Cell_membrane   (765 words)

  
 Biogenesis of the outer membrane
LPS is synthesized at the inner leaflet of the inner membrane and transported to the outer leaflet via the ABC transporter MsbA.
The subsequent transport across the periplasm and to the outer leaflet of the outer membrane is under investigation.
Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface.
www.bio.uu.nl /~microbio/prokaryotes/pro_outermembrane_project_text.htm   (424 words)

  
 EID Vol 3 No 1: Surface Antigens of the Syphilis Spirochete and Their Potential as Virulence Determinants
Further support for the selectivity of the outer membrane isolation was the complete absence of a previously characterized 19-kDa periplasmic protein termed 4D (15) and the abundant 47-kDa lipoprotein (17) and the presence of only trace amounts of endoflagellar protein.
One explanation for the higher molecular mass outer membrane form is its possible association with lipopolysaccharides, known to form a complex with gram-negative outer membrane porin proteins (45).
Of the three outer membrane protein species identified (28-, 31-, and 65-kDa), the 65-kDa protein, tentatively termed Tromp3, is present in the least amount on the basis of 2-dimensional SDS-PAGE gold-stained immunoblots of purified outer membrane (23).
www.cdc.gov /ncidod/EID/vol3no1/blanco.htm   (5558 words)

  
 Webvision: Photoreceptors
It is from the base of the cilium that membrane evaginations and invaginations occur to produce the outer segment (o.s.) or the important visual pigment-bearing portion of the photoreceptor.
In the case of the cones though, the outer segment discs remain attached to the outer segment membrane.
The opsin and the chromophore are bound together and lie buried in the membranes of the outer segment discs.
webvision.med.utah.edu /photo1.html   (1648 words)

  
 The Living Cell - Outer Envelope - Program Guide
Gradually, the amoeba’s outer membrane closes around the prey until it is trapped in a food vacuole.
As much as 40% of the outer membrane is converted into food vacuoles during intensive feeding.
The hormones are released from the membrane to do their job, and just as in phagocytosis and pinocytosis, the membrane patch is returned and merges back into the cell’s outer membrane along with its receptor molecules.
ebiomedia.com /prod/LC/LCenvelope.html   (1737 words)

  
 The Mechanics of Hearing - Membrane People
As fluid moves around in the cochlea because of air-pressure induced vibrations in the outer and middle ear, it deflects the stereocilia on inner hair cells, causing the cells to send an auditory nerve signal to the brain.
Because outer hair cells are rooted alongside inner hair cells in the cochlea’s basilar membrane, outer hair cell contractions are in turn sensed by inner hair cells.
Prestin is lodged in the plasma membrane (blue) of outer hair cells; the rest of the cellular scaffolding consists of spectrin and actin molecules (red and purple).
www.rice.edu /sallyport/2005/spring/features/hearing/membrane.html   (999 words)

  
 Two novel proteins in the mitochondrial outer membrane mediate {beta}-barrel protein assembly -- Ishikawa et al. 166 ...
membrane, and the translocator of the mitochondrial inner membrane
Depletion of Tom13 or Tom38 affects assembly of Tom40 and porin in the mitochondrial outer membrane in vitro.
Biogenesis of the protein import channel Tom40 of the mitochondrial outer membrane: intermembrane space components are involved in an early stage of the assembly pathway.
www.jcb.org /cgi/content/full/166/5/621   (3696 words)

  
 VDAC, a Channel in the Outer Mitochondrial Membrane
When added to mitochondria with intact outer membranes, the VDAC modulator reduces the permeability of the outer membrane and inhibits mitochondrial metabolic activities that require the flux of metabolites through the outer membrane (Liu and Colombini, 1992a).
The binding of hexokinase to outer membranes is thought to increase the accessibility of mitochondrially generated ATP to the enzyme, and hexokinase bound to mitochondria preferentially uses mitochondrial, as opposed to exogenous ATP (Rasschaert and Malaisse, 1990).
-NADH decreases the permeability of the mitochondrial outer membrane to ADP by a factor of 6, J.
www.life.umd.edu /VDAC/web/review/review.html   (11878 words)

  
 Nuclear Envelope
The outermost membrane is continuous with the rough endoplasmic reticulum (ER) and has ribosomes attached (see figure to the left).
The inner and outer membranes of the nuclear envelope are joined and there appears to be a diaphragm-like structure in the center.
The membranes are cleaved along their lipid bilayer and either the face next to the cytoplasm (protoplasmic or P face) or the extracellular (E) face of the membrane is shown.
cellbio.utmb.edu /cellbio/nuclear_envelope.htm   (1744 words)

  
 BH3 Peptide Promotes bax membrane insertion and outer membrane permeabilization in neural CELL mitochondria   (Site not responding. Last check: 2007-10-19)
In apoptotic cells, Bax is oligomerized and inserted into the mitochondrial outer membrane.
No mitochondrial swelling or sensitivity to inner membrane permeability transition pore inhibitors was associated with cyt c release, indicating the permeability of the outer but not the inner mitochondrial membrane was affected.
These results demonstrate that the BH3 minimal death domain is sufficient to cause Bax insertion and release cyt c and support the hypothesis that the formation of a Bax pore in the mitochondrial outer membrane is required for cyt c release by BH3 proteins.
www.umm.edu /anesthesia/brian01.html   (302 words)

  
 OmpT: Molecular Dynamics Simulations of an Outer Membrane Enzyme Biophysical Journal - Find Articles
The trajectories of water molecules reveal exchange of waters between the intracellular face of the membrane and the interior of the barrel but no exchange at the extracellular mouth.
Bacterial outer membrane proteins (OMPs) all share a common architecture, that of a transmembrane domain formed by an antiparallel β-barrel.
Other transport proteins in outer membranes include those for ferric ions (Locher et al., 1998; Ferguson et al., 1998; Buchanan et al., 1999) and for vitamin B12 (Chimento et al., 2003), and export pathways for polypeptide toxins and hydrophobic drugs such as TolC (Koronukis et al., 2000).
www.findarticles.com /p/articles/mi_qa3938/is_200411/ai_n9463162   (921 words)

  
 Model of the Outer Membrane Potential Generation by the Inner Membrane of Mitochondria -- Lemeshko 82 (2): 684 -- ...
The cation-selective substate of the mitochondrial outer membrane pore: single-channel conductance and influence on intermembrane and peripheral kinases.
The cationically selective state of the mitochondrial outer membrane pore: a study with intact mitochondria and reconstituted mitochondrial porin.
Colombini, M. A candidate for the permeability pathway of the outer mitochondrial membrane.
www.biophysj.org /cgi/content/full/82/2/684   (4531 words)

  
 Leptospiral outer membrane proteins (US5989547)
This invention presents antigenic preparations of Leptospira species outer membrane proteins and their immunogenic fragments which are useful for inducing immune response in animals, e.g., for use as vaccines against diseases caused by leptospirosis.
Blanco, et al., "Isolation of the Outer Membranes from Treponema pallidum and Treponema vincentii", J. Bacteriol., 176:6088-6099 (1994).
Radolf, et al., "Characterization of Outer Membranes Isolated from Borrelia burgdorferi, the Lyme Disease Spirochete", Infect.
www.delphion.com /details?pn=US05989547__   (710 words)

  
 Taz1, an Outer Mitochondrial Membrane Protein, Affects Stability and Assembly of Inner Membrane Protein Complexes: ...
of the outer mitochondrial membrane and that the soluble C-terminal
of the outer mitochondrial membrane that exposed its soluble
Wiedemann, N., Truscott, K. N., Pfannschmidt, S., Guiard, B., Meisinger, C., and Pfanner, N. Biogenesis of the protein import channel Tom40 of the mitochondrial outer membrane: intermembrane space components are involved in an early stage of the assembly pathway.
www.molbiolcell.org /cgi/content/full/16/11/5202   (5853 words)

  
 Outer membrane - Wikipedia, the free encyclopedia
The outer membrane refers to the outside membranes of Gram-negative bacteria, the chloroplast, or the mitochondria.
The outer membrane of Gram-negative bacteria has an unusual structure.
The outer leaflet of the membrane comprises a complex lipopolysaccharide whose lipid portion acts as an endotoxin.
en.wikipedia.org /wiki/Outer_membrane   (153 words)

  
 Binding of Iron-free Siderophore, a Common Feature of Siderophore Outer Membrane Transporters of Escherichia coli and ...   (Site not responding. Last check: 2007-10-19)
and the hemophore outer membrane receptor HasR of Serratia marcescens
Outer membranes of PAD07 were incubated with (solid line) or without (dashed line) excess of Pch.
After incubation, the membranes were pelleted and resuspended in Tris-HCl buffer at 250 µg/ml, and the emission fluorescence spectrum was monitored.
www.jbc.org /cgi/content/full/280/21/20222   (5567 words)

  
 Characterization of a Predominant Immunogenic Outer Membrane Protein of Riemerella anatipestifer -- Subramaniam et al. ...
the outer membrane (imp-OmpA), and (iii) mature OmpA (14).
Cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from Bordetella avium and expression of the gene in Salmonella typhimurium.
Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of Escherichia coli K-1.
cdli.asm.org /cgi/content/full/7/2/168   (4316 words)

  
 Bacterial outer membrane vesicles and the host-pathogen interaction -- Kuehn and Kesty 19 (22): 2645 -- Genes and ...
Hellman, J. and Warren, H.S. Outer membrane protein A (OmpA), peptidoglycan-associated lipoprotein (PAL), and murein lipoprotein (MLP) are released in experimental Gram-negative sepsis.
Outer membrane protein A, peptidoglycan-associated lipoprotein, and murein lipoprotein are released by Escherichia coli bacteria into serum.
Stephens, D.S., Edwards, K.M., Morris, F., and McGee, Z.A. Pili and outer membrane appendages on Neisseria meningitidis in the cerebrospinal fluid of an infant.
www.genesdev.org /cgi/content/full/19/22/2645   (6285 words)

  
 Identification of Novel Antigenic Proteins in a Complex Anaplasma marginale Outer Membrane Immunogen by Mass ...
Identification of Novel Antigenic Proteins in a Complex Anaplasma marginale Outer Membrane Immunogen by Mass Spectrometry and Genomic Mapping -- Lopez et al.
of the composition of the outer membrane immunogen is limited.
OMP by use of IgG serum antibody from outer membrane vaccinates.
iai.asm.org /cgi/content/abstract/73/12/8109   (352 words)

  
 Membrane Proteins of Known Structure   (Site not responding. Last check: 2007-10-19)
But the active site is external to the membrane, and many other residues on the protein surface contribute to membrane binding.
Absence of the TM segment affects neither membrane association or function.
FptA, pyochelin outer membrane receptor: Pseudomonas aeruginosa, 2.0 Å
blanco.biomol.uci.edu /Membrane_Proteins_xtal.html   (475 words)

  
 Biology4Kids.com: Cell Structure: Cell Membrane
The cell membrane is not one solid piece.
The membrane that surrounds a lysosome is different from the membrane around the endoplasmic reticulum.
While we talk about membranes all the time, you should remember they all use a basic phospholipid bilayer, but have many other different parts.
www.biology4kids.com /files/cell_membrane.html   (409 words)

  
 Negative Regulation of the Pseudomonas aeruginosa Outer Membrane Porin OprD Selective for Imipenem and Basic Amino ...
Outer membranes were prepared from cultures grown to an optical density at 600 nm of 1.0 by sucrose gradient density centrifugation
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins (A) and Western immunoblots of outer membrane proteins with an anti-OprD MAb (B) and an anti-OprN MAb (C).
Western immunoblots of outer membrane proteins of strain H103 grown in Mueller-Hinton medium with 16 mM benzoate (lane 1) or 32 mM salicylate (lane 2) or without any additives (lane 3).
aac.asm.org /cgi/content/full/43/5/1085   (4699 words)

  
 A simple statistical method for discriminating outer membrane proteins with better accuracy -- Gromiha and Suwa 21 (7): ...
Chimento, D.P., Kadner, R.J., Wiener, M.C. (2003b) The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation.
Schulz, G.E. (2002) The structure of bacterial outer membrane proteins.
Vogt, J. and Schulz, G.E. (1999) The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence.
bioinformatics.oxfordjournals.org /cgi/content/full/21/7/961   (4585 words)

  
 Insecticidal Activity Associated with the Outer Membrane Vesicles of Xenorhabdus nematophilus -- Khandelwal and ...
Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion.
Export of virulence genes and Shiga toxin by membrane vesicles of Escherichia coli O157:H7.
Characterization and environmental regulation of outer membrane proteins in Xenorhabdus nematophilus.
aem.asm.org /cgi/content/full/69/4/2032   (3255 words)

  
 Fishing new proteins in the twilight zone of genomes: The test case of outer membrane proteins in Escherichia coli K12, ...
Fishing new proteins in the twilight zone of genomes: The test case of outer membrane proteins in Escherichia coli K12, Escherichia coli O157:H7, and other Gram-negative bacteria -- Casadio et al.
Fishing new proteins in the twilight zone of genomes: The test case of outer membrane proteins in Escherichia coli K12, Escherichia coli O157:H7, and other Gram-negative bacteria
chains, 327 inner membrane proteins, and 10 outer membrane proteins.
www.proteinscience.org /cgi/content/abstract/12/6/1158   (469 words)

  
 BOMP: a program to predict integral {beta}-barrel outer membrane proteins encoded within genomes of Gram-negative ...
Koebnik,R., Locher,K.P. and Van Gelder,P. ((2000)) Structure and function of bacterial outer membrane proteins: Barrels in a nutshell.
Struyve,M., Moons,M. and Tommassen,J. ((1991)) Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein.
Phadke,N.D., Molloy,M.P., Steinhoff,S.A., Ulintz,P.J., Andrews,P.C. and Maddock,J.R. ((2001)) Analysis of the outer membrane proteome of Caulobacter crescentus by two-dimensional electrophoresis and mass spectrometry.
nar.oxfordjournals.org /cgi/content/full/32/suppl_2/W394   (3335 words)

  
 ecce/ outer membrane/by size
attached to the outer membrane by a lipid anchor
Outer membrane receptor for Fe -coprogen, Fe -ferrioxamine B and Fe -rhodotorulic acid
appears to form a channel in the outer membrane through wich pilin subunits are transported to the surface
www.cf.ac.uk /biosi/staff/ehrmann/tools/ecce/OMPbysize.html   (1121 words)

  
 ecce/ outer membrane/enzymes
integral membrane protein of the inner and the outer membrane
attached to the outer membrane by a lipid anchor (probable)
attached to the outer membrane by a lipid anchor and exposed to the periplasmic side (probable)
www.cf.ac.uk /biosi/staff/ehrmann/tools/ecce/EnzymesOM.html   (238 words)

  
 SCOP: Family: Outer membrane protein transport protein (Pfam 03349)   (Site not responding. Last check: 2007-10-19)
Family: Outer membrane protein transport protein (Pfam 03349)
Class: Membrane and cell surface proteins and peptides [56835]
Family: Outer membrane protein transport protein (Pfam 03349) [111361]
scop.berkeley.edu /data/scop.b.g.dg.f.e.A.html   (96 words)

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