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Topic: Ovalbumin


In the News (Mon 24 Jun 19)

  
  Ovalbumin   (Site not responding. Last check: 2007-10-22)
Ovalbumin is the main protein component of egg whites.
Ovalbumin is broken down into its component amino acids by other proteins called proteases in the process of prolysis.
However, as of late June 2001, research was being undertaken for reasons unknown to modify ovalbumin to return its inhibitory properties by replacing a missing loop within a ß-sheet.
www.bergen.org /ACADEMY/Bio/molbio/AAMB5_Lesson/STORAGE1/Ovalbumin.html   (292 words)

  
 Methods for increasing the circulation half-life of protein-based therapeutics - Patent 5780054   (Site not responding. Last check: 2007-10-22)
The modified ovalbumin was then incubated with liposomes (see section (a) for preparation of liposomes having a reactive functionality on the liposome surface (1% MPB)) at a ratio of 90.mu.g of protein per,umole lipid (15 mM final lipid concentration).
The amount of ovalbumin coupled to the liposomes was determined using the Pierce Micro BCA assay in the presence of 1% Triton X-100 to disrupt the liposomes.
For the latter two groups, liposomes (without surface associated ovalbumin) were administered i.v at a lipid dose of 100 mg/kg, 4 hrs prior to administration of the ovalbumin Freund's mixture.
www.freepatentsonline.com /5780054.html   (7869 words)

  
 Gelation Properties of Albumen Proteins, Singly and in Combination
Therefore, in combinations of lysozyme, globulins, and ovalbumin with conalbumin, denaturation was apparent at 58.3, 57.8, and 58.1 C, respectively.
Ovalbumin coagulation temperature ranged from 71.5 to 71.0 C. Aggregation of the polypeptides proceeded with a modest drop of.5 C in temperature.
The binary mixtures of lysozyme, globulins, ovomucoid, conalbumin, and ovalbumin had significant effects on coagulation temperature, gel strength, and percentage drainage of the coagulums (Table 2).
albumen.stanford.edu /library/c20/johnson1981.html   (3530 words)

  
 Respiratory Research | Full text | Quantification of collagen and proteoglycan deposition in a murine model of airway ...   (Site not responding. Last check: 2007-10-22)
However, in the airways of ovalbumin sensitised and challenged mice, the staining appeared more intense, the band of staining was thickened compared with controls and usually appeared continuous around all sizes of airway (compare figure 2D with figures 2A2B2C).
The current protocol is based on the widely used ovalbumin sensitisation and challenge method [25,26,37,38] using two intra-peritoneal sensitising injections and six daily intra-tracheal challenges of ovalbumin.
The increase in sub-epithelial collagen in ovalbumin sensitised/challenged mice that we found using six consecutive challenges over six days is similar to that observed in other acute [25] and more chronic (3–12 weeks) [26,28,30,33] ovalbumin challenge studies.
respiratory-research.com /content/6/1/30   (6926 words)

  
 Heat-induced denaturation and aggregation of ovalbumin at neutral pH described by irreversible first-order kinetics -- ...
The heat-induced conversion of ovalbumin monomers to aggregates
For DSC scans at an ovalbumin (WCFS) concentration of 1 g/L, a Microcal MC-2 calorimeter was used.
Characterization of linear polymers induced by thermal denaturation of ovalbumin.
www.proteinscience.org /cgi/content/full/12/12/2693   (4915 words)

  
 Identification of the Novel Player delta EF1 in Estrogen Transcriptional Cascades -- Chamberlain and Sanders 19 (5): ...   (Site not responding. Last check: 2007-10-22)
and culminates in induction of the ovalbumin gene.
The ovalbumin probe was incubated with 8 µg of oviduct nuclear protein extract (lanes 2 to 4) or with in vitro-transcribed-translated
Regulation of the chick ovalbumin gene by estrogen and corticosterone requires a novel DNA element that binds a labile protein, Chirp-1.
mcb.asm.org /cgi/content/full/19/5/3600   (4465 words)

  
 Endotoxin Contamination of Ovalbumin Suppresses Murine Immunologic Responses and Development of Airway Hyper-reactivity ...
Endotoxic activity is present in commercial ovalbumin, and is resistant to sequestration by polymyxin B., endotoxic activity was determined with a Limulus amebocyte lysate assay as describe under "Materials and Methods." The amount of activity in endotoxin free PBS, O111:B4 E. coli LPS (LPS(E); 100 ng/ml) or commercial ovalbumin (cOVA; 3 µg/ml) is shown.
purified ovalbumin, in the absence of a concomitant inflammatory
to ovalbumin among inbred strains of mice (32).
www.jbc.org /cgi/content/full/278/43/42361   (5287 words)

  
 Fine mapping and structural analysis of immunodominant IgE allergenic epitopes in chicken egg ovalbumin -- Mine and ...
Synthetic overlapping peptides, corresponding to the sequence of ovalbumin, were synthesized on cellulose membranes and bound with sera from the pool of egg-allergic patients.
The numbers on the left of the figure indicate the position of the amino acid sequence in the native ovalbumin.
The open boxes represent the total number of amino acid residues found in the immunodominant epitopes and the closed boxes represent the number of amino acids which were found to be critical.
peds.oxfordjournals.org /cgi/content/full/16/10/747   (2759 words)

  
 Chicken Ovalbumin (Egg albumin) ELISA Kit cat #6010,
ADI has developed a very sensitive ELISA kit for the detection and quantitation of ovalbumin in animal sera, egg extracts or other biological samples that may contain or suspected of containing ovalbumin.
Residual quantities of egg proteins found in the influenza vaccine (i.e., about 0.02-1.0 ug per dose) are sufficient to induce severe and rarely fatal hypersensitivity reactions in children with egg allergies.
ADI has developed an ELISA kit detecting ovalbumin using polyclonal antibodies, which is of growing importance in standardization and characterization of allergens (ovalbumin) and as a tool for the detection and quantification of egg albumin in food products.
www.4adi.com /kits/animals/6010.html   (457 words)

  
 Ovalbumin peptide encapsulated in poly(d,l lactic-co-glycolic acid) microspheres is capable of inducing a T helper type ...   (Site not responding. Last check: 2007-10-22)
Ovalbumin peptide encapsulated in poly(d,l lactic-co-glycolic acid) microspheres is capable of inducing a T helper type 1 immune response.
An ovalbumin (OVA) peptide, consisting of residues 323-339, was incorporated into poly(d,l lactic-co-glycolic acid) (PLGA) microspheres and administered to mice.
It was hypothesized that microencapsulation of the peptide in PLGA microspheres would avoid the need for traditional adjuvants and bias the immune response towards a type 1 T helper (Th1) response.
www.aegis.com /aidsline/1999/jan/A9910747.html   (473 words)

  
 Goblet Cell Degranulation after Antigen Challenge in Sensitized Guinea Pigs . Role of Neutrophils -- AGUSTI et al. 158 ...
Effect of ovalbumin instillation (2%, 150 µl) in the trachea on goblet cell degranulation (expressed as percentage area of tracheal epithelium stained by Alcian blue/PAS) (upper panel) and on neutrophil recruitment in the trachea (expressed as the number of neutrophils per millimeter of epithelium) (lower panel) in ovalbumin-sensitized guinea pigs.
Compared with control animals sensitized with ovalbumin and given saline instillation alone (150 µl) (diagonally hatched columns), ovalbumin instillation (open columns) caused goblet cell degranulation and neutrophil recruitment.
In the present study, instillation of antigen (ovalbumin) in the trachea of sensitized guinea pigs caused profound goblet
ajrccm.atsjournals.org /cgi/content/full/158/4/1253   (3787 words)

  
 Nano News - Nanorods Deliver Vaccines, Trigger Strong Immune Response
Nanorods comprising two equal segments of nickel and gold, each 800 nanometers long and 170 nanometers in diameter, were decorated with DNA and the protein antigen ovalbumin.
DNA was attached to the nickel segment, while ovalbumin was attached to the gold segment.
Mice were then immunized with the resulting nanorods using a gene gun, which forces the particles through the outer layers of the skin to a layer that is rich with antigen-processing cells.
nano.cancer.gov /news_center/nanotech_news_2005-05-31b.asp   (364 words)

  
 Identification of Proteins Electroblotted to Polyvinylidene Difluoride Membrane by Combined Amino Acid Analysis and ...
Thirty-eight participating laboratories analyzed a known control (ovalbumin, 5 µg applied to the gel) and either lysozyme or bovine serum albumin as unknown samples (1-, 5-, and 10-µg amounts applied to the gel).
Chick ovalbumin and chick egg white lysozyme were purchased from Sigma (www.sigma.com); bovine serum albumin (BSA) was obtained from the National Bureau of Standards.
This is a sample-related issue, because the groups receiving the two unknowns obtained the same accuracy from analyses of the ovalbumin control (16.9% and 17.2% error, respectively).
www.abrf.org /JBT/1999/September99/sep99hunziker.html   (3147 words)

  
 Antigen-Induced Eosinophilic Lung Inflammation Develops in Mice Deficient in Chemokine Eotaxin -- Yang et al. 92 (10): ...   (Site not responding. Last check: 2007-10-22)
The ovalbumin (OVA) challenge of mice was performed as described before.
Eotaxin was originally purified from BAL fluid of guinea pig sensitized and challenged with aerosol ovalbumin.
Frozen sections of lung, thymus, and lymph nodes from untreated or ovalbumin aerosol-challenged (OVA 24 hours) mice were stained with X-gal overnight followed by counter-stain with eosin.
www.bloodjournal.org /cgi/content/full/92/10/3912   (6883 words)

  
 Worthington Ovalbumin   (Site not responding. Last check: 2007-10-22)
Ovalbumin is a glycoprotein with molecular weight of 45 kDa.
The molecule consists of a polypeptide with up to two phosphate groups per mole and a side chain of mannose and glucosamine residues.
Major protein of egg white, with a molecular weight of 43,000.
www.worthington-biochem.com /OA/cat.html   (55 words)

  
 Enhancement of Nasal Inflammatory and Epithelial Responses after Ozone and Allergen Coexposure in Brown Norway Rats -- ...
A mixed inflammatory cell infiltrate of mononuclear cells (e.g., lymphocytes, plasma cells) and eosinophils (arrows) are present in the subepithelial lamina propria and in the basal aspect of the respiratory epithelium (e) in (B) and (C).
Rats were exposed for 3 consecutive days to filtered air (0 ppm ozone) and intranasal saline (A and E), 0.5 ppm ozone and intranasal saline (B and F), filtered air and ovalbumin in saline (C and G), or 0.5 ppm ozone and ovalbumin in saline (D and H).
The epithelial alterations caused by ozone, ovalbumin, and the
toxsci.oxfordjournals.org /cgi/content/full/67/2/284   (6307 words)

  
 Allergen Immunotherapy Inhibits Airway Eosinophilia and Hyperresponsiveness Associated with Decreased IL-4 Production ...
in serum, of which 80% were ovalbumin specific (7).
Ovalbumin, 2,2'-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid), and o-phenylenediamine were purchased from the Sigma Chemical
Repeated ovalbumin inhalation causes bronchial hyperresponsiveness and eosinophil infiltration in sensitized mice.
ajrcmb.atsjournals.org /cgi/content/full/19/4/622   (3588 words)

  
 Sufficiency of the Reactive Site Loop of Maspin for Induction of Cell-Matrix Adhesion and Inhibition of Cell Invasion: ...
rHIS/FLAG maspin, ovalbumin, the maspin/ovalbumin or the RSL in Transwell insert chambers precoated with Matrigel.
Conversion of inactive ovalbumin to a maspin-like molecule by
The ovalbumin serpins revisited: Perspective from the chicken genome of clade B serpin evolution in vertebrates
www.jbc.org /cgi/content/full/278/34/31796   (5895 words)

  
 Disruption of guinea pig urinary bladder permeability barrier in noninfectious cystitis -- Lavelle et al. 274 (1): 205 ...
exposure to ovalbumin, and the apical surface of the bladder is
ovalbumin was instilled for 30 min as described above.
Initially, we analyzed the urothelium of unsensitized, control animals whose bladders were exposed to ovalbumin.
ajprenal.physiology.org /cgi/content/full/274/1/F205   (5515 words)

  
 Ovalbumin antibodies from RDI Divison of Fitzgerald Industries Intl
Background: reacts with ovalbumin from hen egg white showing a 45kDa band under non-reducing and reducing conditions of SDS-Page.
Ovalbumin is the main protein found in egg white and its amino acid content is very similar to that of bovine serum albumin.
The antibody may facilitate the analysis of ovalbumin conjugates used in drug and pharmaceutical manufacturing and in biotechnology, including quantitation methods.
www.researchd.com /miscabs/ovalbab.htm   (943 words)

  
 Presentation of Exogenous Protein Antigens on Major Histocompatability Complex Class I Molecules by Dendritic Cells: ...
The presentation of an epitope derived from soluble ovalbumin was transporter associated with antigen presentation (TAP)-dependent, brefeldin A-sensitive, blocked by inhibitors of proteasomes, and resistant to chloroquine.
To determine the kinetics of ovalbumin presentation, bmDC were incubated with soluble protein for different lengths of time and used as targets in a standard Cr-release assay.
The presentation of these antigens as demonstrated for bacteria-associated or Latex-bound ovalbumin and viral antigens was not blocked by brefeldin A or by a mutation in the TAP transporter.
www.bloodjournal.org /cgi/content/full/90/4/1594   (5485 words)

  
 Heat shock fusion proteins as vehicles for antigen delivery into the major histocompatibility complex class I ...
The peptides SIINFEKL (corresponding to ovalbumin amino acids 258-276) and RGYVYQGL (corresponding to the vesicular stomatitis
or with 120 pmol of hsp70 mixed with 120 pmol of ovalbumin.
Examination of ovalbumin-specific T cell responses in mice injected with a mixture of ovalbumin and hsp70.
www.pnas.org /cgi/content/full/94/24/13146   (5387 words)

  
 BCG infection suppresses allergic sensitization and development of increased airway reactivity in an animal model.
The purpose of this study was to test the hypothesis whether a preexisting Th1-type immune response elicited by BCG immunization could suppress allergic sensitization and airway hyperreactivity in an animal model.
METHODS: BALB/c mice were immunized with BCG and/or sensitized to ovalbumin.
Ovalbumin sensitization alone resulted in increased production of IL-4 after activation by ConA.
www.aegis.com /aidsline/1999/feb/A9920499.html   (435 words)

  
 Assessment of the Allergic Potential of Food Protein Extracts and Proteins on Oral Application Using the Brown Norway ...
Young Brown Norway rats were exposed to either various purified allergenic proteins (e.g., ovalbumin, partly purified), a whole food (cow's milk), or total protein extracts (hen's egg white, peanut) by daily gavage dosing during 42 days without the use of an adjuvant.
This is in line with the knowledge that the BN rat is a high-immunoglobulin (particularly IgE) responder strain and thus, to a certain degree, resembles atopic humans in their genetic predisposition to react more readily with an IgE production to antigens.
Examination of oral sensitization with ovalbumin in Brown Norway rats and three strains of mice.
www.ehponline.org /members/2003/5704/5704.html   (5859 words)

  
 Class I Major Histocompatibility Complex Presentation of Antigens That Escape from the Parasitophorous Vacuole of ...
Ovalbumin presentation activity was measured by using a LacZ B3Z hybridoma assay.
In this assay a T-cell hybridoma that is reactive to an ovalbumin peptide (OVA
Ovalbumin presentation in the populations indicated was read out by using the B3Z LacZ assay.
iai.asm.org /cgi/content/full/73/2/703   (6050 words)

  
 Preexisting Inflammation Due to Mycobacterium bovis BCG Infection Differentially Modulates T-Cell Priming against a ...   (Site not responding. Last check: 2007-10-22)
T-cell response to ovalbumin administered as an inert
Ovalbumin was used as a model antigen so that responses to the
T-cell epitope of ovalbumin residues 257 to 264 is
iai.asm.org /cgi/content/full/70/4/1957   (4338 words)

  
 An improved murine model of asthma: selective airway inflammation, epithelial lesions and increased methacholine ...   (Site not responding. Last check: 2007-10-22)
Mice were exposed to aerosolised ovalbumin for 30 min/day on three days/week for up to eight weeks with assessment of responses
IgE antibodies to ovalbumin in plasma samples from individual animals were quantified using an enzyme immunoassay.
Exposure of non-immunised mice to aerosolised ovalbumin for six weeks was not associated with any evidence of epithelial abnormalities.
thorax.bmj.com /cgi/content/full/53/10/849   (5290 words)

  
 NP-OVAL (Ovalbumin) - Biosearch Technologies
4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to OVAL (ovalbumin) protein through lysine by amide bonds.
The loadings (conjugation ratios) shown are for lots that were available at the time of publication on the web.
Although we make every effort to provide you with the loading requested, there is no guarantee that these lots are still available.
www.biosearchtech.com /products/details.asp?id=416   (54 words)

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