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Topic: PKa

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In the News (Tue 23 Apr 19)

  BioCarta - Charting Pathways of Life
PKA is a tetramer composed of catalytic subunits and regulatory subunits that repress the catalytic units when they are bound together.
The PKA activation pathway is an example of a signal transduction cascade, in which tying several signaling events together amplifies the original signal in the cell.
One substrate of PKA is phosphorylase kinase, which is activated phosphorylation and is part of the signaling cascade that mobilizes glycogen into glucose to provide energy that fuels the response to epinephrine.
www.biocarta.com /pathfiles/gsPathway.asp   (471 words)

 Acid dissociation constant - Wikipedia, the free encyclopedia
Measurements are at 25ºC in water, except for those with a pKa below -1.76:
pKa calculation software, first principle method based on Quantum Mechanics and Poisson-Boltzmann solvation model
This page was last modified 09:28, 29 August 2006.
en.wikipedia.org /wiki/Acid_dissociation_constant   (798 words)

 PKA Karate Academy Rules of the School   (Site not responding. Last check: 2007-10-31)
PKA Karate Academy, Inc. does reserve the right to suspend, or dismiss students and/or terminate training agreements at anytime.
PKA also has a Junior Black Belt Program where a child will move through the advanced levels and achieve their initial Junior Black Belt, then return to learn the adult material and at a later date upgrade to their 1 st Degree Black Belt level.
PKA is excited to offer a special option for individuals wishing to create an income and train for free by hosting private classes (kickboxing or self-defense) or training “parties” with their friends.
www.pkakarate.com /faq.htm   (1730 words)

 Adeno-Associated Virus Type 2 Rep78 Inhibition of PKA and PRKX: Fine Mapping and Analysis of Mechanism -- Schmidt et ...
and inhibit the cAMP-dependent kinases PKA and PRKX.
Inhibition of PKA and PRKX by Rep-derived peptides.
PKA (8 ng) was incubated for 15 min at ambient temperature with various amounts of fluorescently labeled kemptide in the absence (squares) or presence (triangles) of MBP-Rep78 (K340H) (300 ng).
jvi.asm.org /cgi/content/full/76/3/1033   (5224 words)

 pKa Introduction
The structure may be submitted as either a MOL file or SD file and it is not necessary for the structure to be in a three dimensional format or to be optimized to a minimum energy confirmation.
The negative log of the acid ionization constant (pKa) is defined as the ability of an ionizable group of an organic compound to donate a proton (H+) in an aqueous media.
The computed quantity is a measure of its apparent pKa, or macroscopic dissociation constant, at equilibrium, normally taken at 25°C. The pKa value of an organic compound arises from the ionization of either an acid or base, at a specific center in the structure.
www.chemsilico.com /CS_prpKa/PKAintro.html   (554 words)

The inactive PKA holoenzyme is activated when cAMP binds to the regulatory subunits which induces the release of two active catalytic subunits.
AKAPs place PKA type II in proximity with neurotransmitter-activated adenylate cyclase, neurotransmitter receptors for desensitization, ion channels and substrates in the cytoskeleton such as microtubule-associated proteins.
The heatstable protein kinase inhibitor PKI is a key role player for the regulation of PKA activity and for localizing the catalytic subunit of PKA in the cell, also working as a nuclear scavenger.
www.proteinkinase.de /html/pka.html   (1679 words)

 pKa calculations
The pKa value of a titratable group is a measure of the free energy difference between the neutral and charged state of the group.
We now define a quantity called the intrinsic pKa as the pKa that each residue would have if all other titratable groups in the protein were kept fixed in their neutral state.
The pKa values of the active site residues Asp 231, Glu 261 and Asp 328 are highly dependent of a number of other titratable groups that also must be "selected" in order for the pKa values of the active site groups to be accurate.
enzyme.ucd.ie /Science/pKa/pKa_introduction   (4896 words)

PKA members are professional knifemakers, purveyors of custom knives, and suppliers to the trade, who derive a significant part of their income from the making and sale of handcrafted knives.
The purpose of the PKA is to elevate awareness of the art and science of handcrafted knives, provide educational opportunities for those interested in making custom knives, and to promote the commercial and representational interests of its membership.
The PKA firmly believes the interests of its membership are best served by our efforts to acquaint the public with those qualities which so clearly distinguish the custom, handcrafted knife from all others.
www.proknifemakers.com   (356 words)

 Labour Market Pensions
PKA fund contributions range from 12 to 18%.
Members are employed by many different employers - in PKA pension funds predominantly the County, or local authorities or the State.
PKA is therefore similar to an insurance company and is subject to EU life directives and national insurance legislation.
www.pka.dk /public/forside/this_is_pka/labour_market_pensions.htm   (480 words)

 careers at pka technologies
PKA Technologies values diversity and seeks qualified individuals at all levels of our organization.
PKA Technologies is a HP Enterprise reseller located in the Northeast.
PKA Technologies is searching for (4) top notch Account Managers (all new positions due to growth) to join our Sales Teams in our Suffern, NY office.
www.pkatech.com /careers.php   (746 words)

 Physiological ionization and pKa prediction
Although pKa prediction itself is an interesting challenge, the closely related problem of physiological ionization state determination is of greater immediate value to computational chemists.
Similarly, a pKa that is close to 7.3, implies that a significant fraction of the molecule may be either protonated or deprotonated, and analysis should be applied to both forms.
Unfortunately, the Achilles heel of Hammett and Taft based pKa prediction is the dependence upon large databases, both for the substituent constants and for the acid/base functional group under consideration.
www.daylight.com /meetings/emug00/Sayle/pkapredict.html   (1802 words)

It was also possible for the operator to hand fit the data to any wavelength and either the bis or mono pKa equation by using the “manfit” GUI located on the bottom left portion of the screen.
The fitted pKa’s are shown as well as the r^2 (or cod) value (which represents the “goodness” of the fit.) The plot shows the raw datapoints in fl with the fitted curve in red.
Below is the identical screen but with a bis pKa example.
www.pharmalabauto.com /pKa/pka.html   (177 words)

 Differential Effects of Haloperidol and Clozapine on [3H]cAMP Binding, Protein Kinase A (PKA) Activity, and mRNA and ...
PKA activity was determined in both particulate and cytosol fractions obtained from the cortex, hippocampus, and striatum
Immunolabeling of catalytic and regulatory subunit isoforms of PKA in cortex, hippocampus, and striatum was determined by
PKA activity was determined in particulate and cytosol fractions of the cortex, hippocampus, and striatum using kemptide,
jpet.aspetjournals.org /cgi/content/full/301/1/197   (6711 words)

Recent results show that high PKA activity cannot counteract the phosphorylation of Fu that depends on the Hh signal but can be inhibited by Ptc, suggesting that Fu indeed acts downstream of Hh and Ptc (Therond, 1996).
Reduced PKA activity in anterior imaginal disc cells leads to cell-autonomous induction of dpp, wg, and ptc transcription that is independent of hh gene activity (Li, 1995)
Pka clones in the anterior margin transform into bristles corresponding to the region anterior to vein 3 (Jiang, 1995) whereas ptc clones in similar regions transform into posterior cells (Tabata, T., 1995).
www.biochem.northwestern.edu /holmgren/Glossary/Protein&genes/PKA.html   (667 words)

 PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter -- Baba ...
tomosyn was incubated with the catalytic subunit of PKA in the
Phosphorylation of tomosyn by PKA in vitro and in vivo.
Involvement of PKA in neurotransmitter release from SCG neurons.
www.jcb.org /cgi/content/full/170/7/1113   (8974 words)

 Introduction to PKA   (Site not responding. Last check: 2007-10-31)
PKA는 구성원 각각이 다양한 전공분야 정보와 취미, 관심사 등을 서로 교류할 수 있는 기회의 장을 마련하고 있습니다.
PKA는 구성원들과 한국의 기업, 연구소, 대학 등과의 가교 역할을 하고 있습니다.
PKA는 Purdue University에서의 학업, 연구활동을 고려하고 있는 분들에게 필요한 정보를 제공하고 있으며, 필요한 경우, 이분들의 현지 정착을 도와드리고 있습니다.
web.ics.purdue.edu /~korean/PKA_introduction.htm   (184 words)

 Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha - PKA-200
The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits.
The recombinant PKA catalytic subunit alfa is a 41kDa protein.
One unit is defined as the amount of recombinant PKA catalytic subunit alfa, required to incorporate 1nmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30°C. Specific Activity; The specific activity of the recombinant PKA catalytic subunit alfa, is >10.000 U/mg.
www.prospec.co.il /~prospec/cart/catalog/rPKAca.html   (546 words)

 about pka technologies, inc.
Whether you sell a product, provide a service, are involved in higher education, or in e-business, PKA is your best choice for your IT infrastructure procurement.
PKA Technologies, Inc. has been supplying IT technology products and services to Fortune 500 companies since 1985.
Over the years PKA Technologies has forged strong alliances with Hewlett-Packard, Hitachi Data Systems, and many other companies.
www.pkatech.com /about.php   (635 words)

 Dual role of PKA in phenotypic modulation of vascular smooth muscle cells by extracellular ATP -- Hogarth et al. 287 ...
PKA activation by ATP is sufficient for their downregulation.
Cell lysates were analyzed for PKA activity by nonradioactive in vitro PKA assay (A) or for ERK1/2 phosphorylation by electrophoretic mobility shift of phosphorylated ERK1 and ERK2 (B).
Inhibition of PKA by adenovirus-mediated transduction of PKI.
ajpcell.physiology.org /cgi/content/full/287/2/C449   (5438 words)

 PKA Photobiotinylation   (Site not responding. Last check: 2007-10-31)
The cAMP dependent protein kinase (PKA) from bovine heart was chosen for this purpose.
A purified PKA catalytic subunit which does not require cAMP for activation and a crude PKA preparation which requires cAMP for regulatory subunit dissociation and subsequent catalytic subunit activation.
PKA was incubated with the photoprobe for 30 sec.
www.altcorp.com /AffinityLabeling/pkaphotobiotinylation.htm   (1284 words)

 PKA Kickboxing History
September 14th, 1974 was the date of the first World Championships promoted by the PKA in Los Angeles Sports Arena, California.
Many great fighters fought through the years under the PKA with the notable inclusion of Benny ‘The Jet’ Urquidez.
The PKA (Professional Karate Association) in the USA is secured by Joe Corley.
www.pka-kickboxing.com /pka-kickboxing-history.html   (442 words)

 Combisep : Applications - pKa   (Site not responding. Last check: 2007-10-31)
The acid dissociation constant (pKa value) of an ionizable compound is defined as the pH value at which the dissociated and undissociated species are of equal concentration.
Experimental knowledge of the pKa value of a new chemical entity (NCE) provides a means to determine the extent of ionization at any solution pH value, and is highly beneficial for predicting compound absorption, distribution, metabolism, and excretion (ADME) properties.
The pKa value also plays an important role in the development of drug delivery formulations.
www.combisep.com /pka.html   (415 words)

 determine drug absorption by pKa
The pKa of sodium pentothal is 7.4 and it is acidic.
2) A basic drug with a pKa of 7.8 is a known teratogen.
pKa is defined as the pH were a drug exists as 50% ionized and 50% unionized
www.manuelsweb.com /pka.htm   (362 words)

 PROPKA   (Site not responding. Last check: 2007-10-31)
The PROPKA method is described in Hui Li, Andrew D. Robertson, and Jan H. Jensen "Very Fast Empirical Prediction and Interpretation of Protein pKa Values" Proteins, 2005, 61, 704-721.
NMR structures are likely to yield inaccurate pKa values for some residues.
Melissa A. Porter, Jordan R. Hall, James C. Locke, Jan H. Jensen and Pablo A. Molina "Amide Backbone Interactions are the Prime Determinants of Carboxyl pKa Values at N-termini of alpha-helices" Proteins: Structure, Function, and Bioinformatics, 63, 621 - 635.
propka.chem.uiowa.edu   (425 words)

 Chronic ethanol downregulates PKA activation and ciliary beating in bovine bronchial epithelial cells -- Wyatt and ...
as ethanol activates PKA in the airway epithelial cell (32).
PKA activity was determined in crude whole cell fractions of BBEC.
There may be several possible explanations for the modulation of PKA by chronic ethanol.
ajplung.physiology.org /cgi/content/full/281/3/L575   (4509 words)

 Bioreagents.com: PKA RII   (Site not responding. Last check: 2007-10-31)
The MA3-517 immunogen is a purified fraction of cAMP dependent PKA RII from human saliva.
The type 1(RI) and type 2 (RII) subunit of cyclic AMP-dependent protein kinase A (PKA) is a polyfunctional protein that is present in most tissues and cells.
Localization of PKA is coordinated through association with A-kinase anchoring proteins (AKAPs) that bind to a 14-18aa sequence in subunit RII.
www.bioreagents.com /index.cfm/fuseaction/products.detail/CatNbr/MA3-517   (210 words)

 pKa Determination
Determination of the dissociation constant for a drug capable of ionization within a pH range of 1 to 10 is important since solubility, and consequently absorption, can be altered by orders of magnitude with changing pH.
The Henderson-Hasselbalch equation provides an estimate of the ionized and un-ionized drug concentration at a particular pH.
pKa of a compound is thus a measure of drug un-ionized at a certain pH
www.pharmquest.com /source/ddg/steps/preclinical/preformulation/pKa_determination/index.html   (126 words)

 IMAP FP PKA Assay Kit
A sensitive, non-radioactive, homogeneous and rapid assay for PKA
The Molecular Devices IMAP FP PKA Assay Kit provides a sensitive and rapid assay that detects the activity of PKA without antibodies in a homogeneous format.
This assay kit is designed for use in drug discovery to screen the activity of PKA in the presence of putative drug compounds.
www.moleculardevices.com /pages/reagents/imap_pka.html   (343 words)

 CAMB: Faculty: Stuart B. Moss   (Site not responding. Last check: 2007-10-31)
Protein kinase A (PKA) is an ubiquitous serine/threonine kinases that is critical for the regulation of sperm motility.
Since AKAPs are proteins that anchor PKA to specific subcellular locations, our working hypothesis is that AKAP4 acts as a scaffolding protein for regulatory proteins in the flagellum.
In contrast to sperm, the mammalian oocyte and blastomeres of the preimplantation embryo are large cells with an abundant amount of cytoplasm.
www.med.upenn.edu /camb/faculty/db/moss.html   (428 words)

 PKA Photolabeling   (Site not responding. Last check: 2007-10-31)
The purpose of these experiments was to demonstrate the dramatic effect that the choice of buffer and pH can have on photolabeling of cAMP dependent protein kinase (PKA).
Never assume the buffer and pH that you have been using in the past to assess catalytic activity will be the same as the optimum conditions for photolabeling.
O was added to a final test concentration of 10 µM and samples gently vortexed to mix.
www.altcorp.com /AffinityLabeling/pkaphotolabeling.htm   (1048 words)

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