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Topic: Patch clamp technique

    Note: these results are not from the primary (high quality) database.

Ion Channel, Patch Clamp & Electrophysiology Resources The patch clamp technique applied to real neurons Very well-made video showing the key components of the patch clamp technique. Screening is done using the IonWorks high-throughput patch clamp device (pictured right) or using conventional patch clamp. www.ionchannels.org   (1275 words)

Whole Cell Patch Clamp Recording Performed on a Planar Glass Chip -- Fertig et al. 82 (6): 3056 -- Biophysical Journal The state of the art technology for the study of ion channels is the patch clamp technique. of these is the patch clamp technique (Sakmann and Neher, 1995 Finally, transferring the patch clamp technique onto a planar device enables a variety of new kinds of experiments on ion www.biophysj.org /cgi/content/full/82/6/3056   (3149 words)

Electrophysiology -- Facts, Info, and Encyclopedia article At the cellular level, these include so-called passive recording as well "patch-clamp" technique, which "clamp" or maintain the (additional info and facts about cell potential) cell potential (voltage-clamp) or current (current-clamp) at a level the experimenter may specify. With this technique it is possible to clamp the cell potential (voltage-clamp) or the cell current (current-clamp) using a glass micropipette as explained previously. Some popular clinical applications of extracellular recording are the (A graphical recording of the cardiac cycle produced by an electrocardiograph) electrocardiogram (ECG) and the (A graphical record of electrical activity of the brain; produced by an electroencephalograph) electroencephalogram (EEG). www.absoluteastronomy.com /encyclopedia/e/el/electrophysiology.htm   (675 words)

Jarett Diamond - Prof. Luke Lee's Lab The patch clamp technique is a widely used method for measuring the electrical currents which flow across the membrane of electrically active cells such as neurons. High-resolution measurements are possible using the patch clamp technique, but using the glass micropipette is a delicate and labor intensive undertaking. While giga-ohm seals were not achieved with this device (indicating incomplete patch formation), it provided important insight into the design of the next generation of planar patch clamp devices. bioeng.berkeley.edu /guidant/guidant2003/studentsites/diamond.html   (289 words)

59447.010816&ELEMENT_SET=DECL The apparatuses and methods of the present invention may also be used for techniques such as, internal perfusion of oocytes, patch clamp electrophysiology, brain slice recording, receptor-ligand interactions on cell surfaces, calcium imaging studies, confocal microscopy, and in vivo microdialysis, for example. The patch clamp technique is the central technique for studying ion channels. In particular, patch electrodes according to the present invention may be distinguished from the patch clamp electrodes of the prior art, in that the electrode partitions in the latter are individually prepared, typically from a glass capillary, to form a micropipette. www.wipo.int /cgi-pct/guest/getbykey5?KEY=01/59447.010816&ELEMENT_SET=DECL   (12641 words)

Fred Sigworth Lab To make the patch clamp technique simpler and more versatile, we are developing planar patch-clamp electrodes which replace the traditional glass micropipettes. The patch clamp technique allows single channel currents to be measured with high precision. The molecular motions involved in the sensing and switching can also be studied by fluorescence techniques. info.med.yale.edu /cmphysiol/sigworth   (400 words)

Yale Scientific Magzine In eletrophysiology, the patch clamp technique is used for measuring these currents to study current-generating ion transport through ion-channel membrane proteins. The new and improved technique will greatly benefit the pharmaceutical industry, where patch clamp is widely used in studying the effects of drugs and medical treatments. Of particular interest is planar patch-clamp technology, a current research focus of Eugenio Culurciello, assistant professor of electrical engineering, and his collaborators James Klemic, associate research scientist of physiology, and Fred Sigworth, professor of physiology and biomedical engineering. research.yale.edu /ysm/article.jsp?articleID=408   (307 words)

Population Patch Clamp Delivers Faster, Cheaper and Better Data - 050310165334 The Population Patch Clamp is a fundamentally new patch clamp technique for screening that defies conventional wisdom and allows for substantially greater throughput at lower cost and better data consistency. A planar population patch clamp will be described in which there are 64 apertures per well. Kinetic responses and IC s are consistent with equivalent single-cell patch clamp. www.sbsonline.org /sbscon/2005/speakers/050310165334.php   (185 words)

Nanion Technologies - Automated Patch Clamp Nanion received the award for its chip-based patch clamp technique, which allows to perform sophisticated electrophysiological measurements from cells in an automated and parallel manner. Nanions entry level device, the Port-a-Patch, supersedes the common pipette based patch clamp technique in ease of use and throughput and doesn’t require a microscope, vibration isolation or a micromanipulator. After a successful introduction of Nanion’s first generation of automated patch clamp devices, the company seeks to expand its team and is very glad to hire Cecilia Farre, Ph.D., for research, product development as well as marketing and sales activities. www.nanion.de /content/papers/news.php   (1079 words)

MENAFN - Middle East North Africa . Financial Network News: Molecular Devices Introduces IonWorks(R) Quattro(TM) System for Accelerated Ion Channel Screening The IonWorks Quattro system was developed using Molecular Devices' Population Patch Clamp(TM) technology, a revolutionary new approach in the planar patch clamp technique. The IonWorks Quattro system employs a new PatchPlate(TM) Population Patch Clamp (PPC) substrate, a 384-well planar array that is designed to allow ion channel recordings to be simultaneously collected from multiple cells within each well. The PPC technique is designed to remove the dependence on the expression levels or viability of individual cells, thereby enabling each well to measure the response to a population of cells." www.menafn.com /qn_news_story.asp?StoryId=CqGRQubWbmdqXCdeXntK   (598 words)

ipedia.com: Alan Lloyd Hodgkin Article Confirmation of ion channels came with the development of the patch clamp, which led to a Nobel prize in 1991 to Erwin Neher and Bert Sakmann. The experimental measurements on which the pair based their action potential theory represent one of the earliest applications of a technique of electrophysiology known as the "voltage clamp". The second critical element of their research was the so-called giant axon of Atlantic squid (Loligo pealei), which enabled them to record ionic currents as they would not have been able to do in almost any other neuron, such cells being too small to study by the techniques of the time. www.ipedia.com /alan_lloyd_hodgkin.html   (243 words)

Sigworth Lab: Patch Clamp The patch clamp technique (Neher, 1992) is the central method in modern electrophysiology. We have been working on materials and designs for "planar" patch clamp electrodes. After unsuccessful attempts with silicon and quartz "chips" with micromachined apertures, we now have been using the well known silicone elastomer PDMS as a material to interface to the cell membrane. info.med.yale.edu /cmphysiol/sigworth/patchcl.html   (218 words)

AVIVA's SealChip(TM) Focus of Three Sessions at Scientific Meeting The traditional patch-clamp technique is a low throughput, labor intensive, and costly manual process. AVIVA Biosciences Corporation announced today its planar patch-clamp biochip, SealChip(TM), was the subject of three scientific presentations at the 47th Annual Meeting of the Biophysical Society, currently being held in San Antonio, Texas. Planar patch-clamp biochips have been developed by AVIVA to improve ion channel research. www.forrelease.com /D20030305/law047.P1.03052003131526.01260.html   (265 words)

Manfred Lindau We investigate single exocytotic fusion events by measurements of membrane capacitance using the patch clamp technique exploiting the fact that exocytosis and endocytosis are associated with changes in plasma membrane area leading to proportional changes of electrical membrane capacitance (11,20,49,51). Such devices will include on-chip electrochemical detector arrays with integrated electronics, incorporation of electrochemical detectors into planar patch clamp devices and electrochemical detector devices to be used in the investigation and treatment of Parkinson's disease. Our methods of patch capacitance measurements allow for the investigation of the opening of single exocytotic fusion pores in neurosecretory vesicles using cell-attached and excised patches (41,46,55) with a resolution similar to that obtained in conventional single channel recordings. www.people.cornell.edu /pages/ml95   (1059 words)

Berthold Leibinger Stiftung The best technology to study ion channel function and screen for compound interaction is the patch clamp technique, but patch clamping suffers from low throughput, which is not acceptable for drug screening. We present patch-clamp recordings in the whole-cell configuration and single channel recordings performed with planar patch-clamp chips, which are microstructured from borosilicate glass substrate by laser ablation. Planar measurement sonde for electrophysiological "Patch clamp on-a-chip" www.leibinger-stiftung.de /107.news5244.html   (167 words)

ISG website:   Electrophysiological recordings of cellular activity using patching clamping is a common laboratory technique. Arrays of embedded onboard silicon patch clamping holes can overcome this limitation, provided the intrinsic capacitance can be minimised and highly resistive seals can be made between silicon and cell membrane. The feasibility of using planar patch-clamp recording devices is highly dependent on the probability of obtaining sealed neurons. gaia.ecs.csus.edu /~changw/205/verilog_manual/index.html.32   (3015 words)

Globeinvestor.com: Axon Instruments Ships 20th PatchXpress System It automates the patch-clamp technique to screen drug libraries for compounds that act on ion-channel targets, allowing hundreds of compounds to be tested with minimal user intervention. In neurosciences, Axon Instruments' patch clamp amplifiers and software are used to study such conditions as anxiety, Alzheimer's disease, Parkinson's disease, heart disease, neuropathic pain, diabetes, autoimmune diseases, cystic fibrosis and migraine. The PatchXpress 7000A, with exclusive use of the Sealchip16(TM) planar electrode from Aviva Biosciences Corp., is the world's first commercially available automated planar patch-clamp workstation for true, tight-seal whole-cell voltage clamp. www.globeinvestor.com /servlet/ArticleNews/print/PRNEWS/20040225/2004_02_25_10_2454_1097972   (460 words)

Biotechnology News and Information Portal, biotech jobs and career resources Resources. The patch clamp technique is widely recognized as the gold standard for monitoring the behavior of ion channels. The company already produces a successful planar patch-clamp electrode device that will be integrated into the PatchXpress systems. Aviva has a proven record of integrating multiple analytical and preparative techniques into disposable microfabricated biochips. www.bioexchange.com /news/news_page.cfm?id=12506   (651 words)

IMT Sensors, Actuators and Microsystems Laboratory The patch clamp technique gives insight in the collective electrical behavior of an ensemble of such proteins. This problem can be overcome by a planar patch clamp support. Atomic force microscopy (AFM) on the other hand, has been applied to measure the topography of membranes and monitor changes at the surface of active membrane proteins, with sub-nanometer lateral resolution. www-samlab.unine.ch /activities/nanobiology.htm   (263 words)

ACS Biosensing University of Luton I've never had to do it myself - but I well remember descriptions of the hell that was necessary to carry out ion channel measurements using the patch clamp technique. Planar Patch Clamp Technologies: Parallel Measurement of Ionic Currents / Individual Live Cells. Well, James had some good news for those of us that didn't already know - now there is a better way of doing it. www.luton.ac.uk /research/lirans/other/acs/acsbio   (636 words)

CGI References Limitations of the whole cell patch clamp technique in the control of intracellular concentrations In "Voltage and Patch Clamping with Microelectrodes" Ed. Matthews B, Judy JW Characterization of a micromachined planar patch clamp for cellular electrophysiology www.see.ed.ac.uk /~s9810372/cgi-bin/display.cgi?order=4   (804 words)

Identifying hERG Modulators with an Automated Patch Clamp System - SBS Conference 2003, Portland Oregon This device uses the gold standard gigohm seal/ruptured whole-cell patch-clamp technique in a sixteen-well planar format in which each well is controlled independently and in parallel. Identifying hERG Modulators with an Automated Patch Clamp System - SBS Conference 2003, Portland Oregon We studied hERG channel activity using the PatchXpress (Axon Instruments). www.sbsonline.org /sbscon/2003/talks/030329011259.htm   (196 words)

Executive Briefing: The Re-Emergence of Ion Channel Drug Discovery The technique involves forming seals where cells come in contact, via suction, with holes in a flat surface through which a patch clamp (a way to calibrate and measure ion flow through a channel) gets applied to the cell. One technology, automated planar patch-clamp, promises to speed up screening of compound libraries against ion channel targets. A variety of automated assay technologies that have become commercially available over the past year should increase throughput by orders of magnitude. www.windhover.com /contents/monthly/exex/e_2003900085.htm   (693 words)

A Novel H+ Conductance in Eosinophils: Unique Characteristics and Absence in Chronic Granulomatous Disease -- Bánfi et al. 190 (2): 183 -- The Journal of Experimental Medicine the current clamp mode of the patch clamp technique. The whole cell patch clamp technique (47) was used to measure The membrane potential of eosinophils, determined as the zero current holding potential, was measured in current clamp mode after the formation of the whole cell configuration (arrowhead). www.jem.org /cgi/content/full/190/2/183   (693 words)

Self-Inhibition in Amiloride-sensitive Sodium Channels in Taste Receptor Cells -- Gilbertson and Zhang 111 (5): 667 -- The Journal of General Physiology or the conventional whole-cell variation of the patch clamp technique Perforated patch voltage clamp recordings were made from isolated Sodium self-inhibition in ASSCs is most evident in patch clamp recordings from isolated rat fungiform TRCs as a decrease in www.jgp.org /cgi/content/full/111/5/667   (693 words)

Whole-Cell Voltage Clamping of Isolated Heart Cells   For an overview of the basics of the whole-cell voltage clamp technique in general, I would advise the beginner to read the classic article by Hamill Whole-cell voltage clamp of isolated heart cells has led to an explosion of information about ionic currents in the heart. The whole-cell configuration is subsequently attained by rupturing the patch of membrane within the pipette by either suction (generally applied by mouth or alternatively by a gas-tight syringe) or by “zapping” the membrane. www.usouthal.edu /ishr/help/wholecellclamp   (693 words)

Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells -- Gemel et al. 117 (12): 2469 -- Journal of Cell Science double whole-cell patch clamp technique as described previously Voltage and current signals were recorded using patch clamp Srinivas, M., Costa, M., Gao, Y., Fort, A., Fishman, G. and Spray, D. Voltage dependence of macroscopic and unitary currents of gap junction channels formed by mouse connexin50 expressed in rat neuroblastoma cells. jcs.biologists.org /cgi/content/full/117/12/2469   (693 words)

The Patch Clamp To measure what's happening in or on a single, living cell, scientists use a technique called the patch clamp which requires an extremely fine pipet held tightly against the cell membrane. Once good contact is made, it is possible to record ion channels opening and closing. By carefully heating and pulling a small glass or quartz capillary tube, a very fine pipet can be formed. www.cellsalive.com /patch.htm   (173 words)

NMDA-Induced Dendritic Oscillations during a Soma Voltage Clamp of Chick Spinal Neurons -- Moore et al. 19 (19): 8271 -- Journal of Neuroscience Sernagor E, O'Donovan MJ (1991) Whole-cell patch clamp recordings from rhythmically active motoneurons in the isolated spinal cord of the chick embryo. Our technique consists of a whole-cell large-signal nonlinear step-voltage clamp on which is superimposed a sum of low-amplitude A whole-cell voltage clamp of the somatic membrane was used to www.jneurosci.org /cgi/content/full/19/19/8271   (173 words)

A 5-HT7 Receptor-Mediated Depolarization in the Anterodorsal Thalamus. II. Involvement of the Hyperpolarization-Activated Current Ih -- Chapin and Andrade 297 (1): 403 -- Journal of Pharmacology And Experimental Therapeutics the blind tight-seal patch clamp technique to examine the ability Slices were incubated in either staurosporine or H-8 for at least 1 h to ensure penetration of the blocker to the cell. Serotonin shifts the voltage dependence of this current in the depolarizing direction by approximately 8 mV. www.jpet.org /cgi/content/full/297/1/403   (173 words)

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