Peripheral membrane protein - Open Encyclopedia(Site not responding. Last check: 2007-10-21)
Peripheralmembraneproteins are proteins that adhere only loosely to the biological membrane with which they are associated.
Therefore the so-called regulatory protein subunits of many ion channels and transmembrane receptors, for example, may be defined as peripheralmembraneproteins.
An exception to this rule is that cells may attach an "anchor" moiety, such as the fatty-acid anchor of lipid anchored proteins, which makes the purification properties of these proteins the same as integral membraneproteins, although researchers tend not to classify them as such.
peripheral membrane protein(Site not responding. Last check: 2007-10-21)
Peripheralmembraneproteins are associated with membranes but do not penetrate the hydrophobic core of the membrane.
For example salt washes of membranes are often sufficient to remove peripheralmembraneproteins whereas detergent treatment of membranes is often required to remove integral membraneproteins.
An example of peripheralmembraneprotein is the cytochrome C in the electron transport chain.
Membrane Protein - The Building Blocks For Muscle Mass(Site not responding. Last check: 2007-10-21)
How membraneproteins are assembled and how to think about thethermodynamics of the stability of membraneproteins...
The Bacteriorhodopsin protein embedded in a portion of phospholipid bilayer membrane is shown using the Cartoon display, colored by Structure.
The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein is coded for by a gene on chromosome 7 and is the affected protein in the inherited disease cystic fibrosis of the pancreas.
Sam35 of the Mitochondrial Protein Sorting and Assembly Machinery Is a Peripheral Outer MembraneProtein Essential for Cell Viability -- Milenkovic et al.
Tim proteins are transferred to the SAM complex (10, 24-27).
peripheralmembraneprotein that is associated with the membrane
Genomically encoded protein A chimeras of Yor193p and the peroxisomal
Equal amounts of protein from the total cell lysates were separated by SDS-PAGE and analyzed by immunoblotting with antibodies to thiolase to visualize the protein A fusion and thiolase.
Pex11p, Pex25p and Pex25p, and Yor193p and Yor193p fusion proteins
membranes and indicates that it is not an integral membrane
Cox, D.N., and Muday, G.K. NPA binding activity is peripheral to the plasma membrane and is associated with the cytoskeleton.
Saint-Jore, C.M., Evins, J., Batoko, H., Brandizzi, F., Moore, I., and Hawes, C. Redistribution of membraneproteins between the Golgi apparatus and endoplasmic reticulum in plants is reversible and not dependent on cytoskeletal networks.
Subsequently, lipid II is translocated across the cell membrane, after which the disaccharide-pentapeptide is transferred to the peptidoglycan network.
Lipid II is an intrinsic component of the pore induced by nisin in bacterial membranes.
Escherichia coli minicell membranes are enriched in cardiolipin.
Loss of myosin VI reduces secretion and the size of the Golgi in fibroblasts from Snell's waltzer mice -- Warner et al. ...(Site not responding. Last check: 2007-10-21)
VI was removed (Figure 4A, c), implying that myosin VI is a peripheralmembraneprotein in the Golgi complex.
Immunoblotting of the Golgi membranes (b) and of the salt-washed Golgi membranes (c) with antibodies to the whole tail of myosin VI.
The last temperature shift causes release of the VSVG protein from the Golgi complex, and it is trafficked towards the plasma membrane.
Pilt, a Novel PeripheralMembraneProtein at Tight Junctions in Epithelial Cells -- Kawabe et al.
The beads were then subjected to SDS-PAGE (10% polyacrylamide gel), followed by Western blotting with the anti-Myc Ab or protein staining with Coomassie Brilliant Blue.
Each fraction (25 µg of protein each) was subjected to SDS-PAGE (10% polyacrylamide gel), followed by Western blotting with the anti-Pilt or anti-hDlg Ab.
