
	Where results make sense

Topic: Periplasm

Related Topics

Cell wall

Peptidoglycan

Plasma membrane

Cell membrane

Bacterium

Uniporter

In the News (Sat 14 Nov 09)

Northern Trust

Marvin Harrison

Peter Chernin

Gary Locke

Match Play

Penelope Cruz

Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Procaryote Cell Walls and Membranes
		The periplasmic space is a volume found between the bacterial plasma membrane and cell wall.
		The substance that occupies the periplasmic space is the periplasm.
		The periplasm in gram-positive cells is thus part of the cell wall." (p.
	www.mansfield.ohio-state.edu /~sabedon/biol1080.htm (2942 words)

	Biophysical Characterization of Changes in Amounts and Activity of Escherichia coli Cell and Compartment Water and ...
		We conclude that the periplasm and cytoplasm are isoosmotic
		The periplasmic volume curve was determined by subtraction of the fitted curves for cell and cytoplasmic volumes.
		Turgor pressure was predicted from the concentration of periplasmic MDO by Eq.
	www.biophysj.org /cgi/content/full/78/4/1748 (7188 words)

	The SecA Subunit of Escherichia coli Preprotein Translocase Is Exposed to the Periplasm -- Eichler and Wickner 180 ...
		(A) The levels of the periplasmically oriented marker proteins leader peptidase (Lep) and outer membrane protein A (OmpA) and of the cytoplasmically localized trigger factor (TF) were determined by immunoblotting of OMP cells in the absence (lane 1) or presence (lane 2) of 1.0 mg of trypsin per ml and 1.0% Triton X-100 (Sigma).
		Evidence that KpsT, the ATP-binding component of an ATP-binding cassette transporter, is exposed to the periplasm and associates with polymer during translocation of the polysialic acid capsule of Escherichia coli K1.
		SecA is an intrinsic subunit of the Escherichia coli preprotein translocase and exposes its carboxyl terminus to the periplasm.
	jb.asm.org /cgi/content/full/180/21/5776 (2765 words)

	Kao-Lu Pan 2003 3020
		However, a shortage of periplasmic protease activity was not the only reason for the deterioration in culture performance upon pac overexpression because coexpression of a DegP-homologous periplasmic protease, DegQ or DegS, could not suppress the physiological toxicity.
		The formation of inclusion bodies, which are composed primarily of PAC precursors in the periplasm, was recently identified as an important obstacle to the overproduction of PAC in E.
		Periplasmic aggregation limits the proteolytic maturation of the Escherichia coli penicillin G amidase precursor polypeptide.
	www.bionewsonline.com /e/p/kao-lu_pan_2003_3020.htm (5354 words)

	The gaf Fimbrial Gene Cluster of Escherichia coli Expresses a Full-Size and a Truncated Soluble Adhesin Protein -- ...
		DE3(pKJ1) periplasm by affinity chromatography and analysis of the reactivities of fimbrial preparations with anti-G-fimbria and anti-GafD antibodies.
		GafD was accumulated in the periplasm concomitantly with
		Periplasmic chaperone recognition motif of subunits mediates quaternary interactions in the pilus.
	jb.asm.org /cgi/content/full/183/2/512 (5165 words)

	Cosecretion of Chaperones and Low-Molecular-Size Medium Additives Increases the Yield of Recombinant Disulfide-Bridged ...
		Yields of native rPA and proinsulin in the periplasm of E.
		Similarly, cosecretion of Hsp25 increased the yield of native rPA in the periplasm ca.
		With proinsulin, a 37-fold increase in the yield of native protein was obtained by cosecretion of DnaJ.
	aem.asm.org /cgi/content/full/67/9/3994 (4088 words)

	Glycerol Conductance and Physical Asymmetry of the Escherichia coli Glycerol Facilitator GlpF -- Lu et al. 85 (5): 2977 ...
		The saturation of the channel flux is due to clogging of the attractive vestibule at the periplasmic side.
		Glycerol population of the vestibule is shown in the inset.
		periplasm and cytoplasm, it corresponds to the glycerol concentration,
	www.biophysj.org /cgi/content/full/85/5/2977 (5647 words)

	Genome Biology \| Full text \| The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those ...
		The established periplasmic enzyme PheC [ 13, 16 ] was used as a periplasmic* protein marker and shikimate dehydrogenase was used as a cytoplasmic protein marker.
		The spatial location of the overflow pathway in the periplasmic compartment was first indicated by the demonstration that cyclohexadienyl dehydratase had a cleavable amino terminus and was translocated to the periplasm.
		aeruginosa : one for aromatic biosynthesis in the cytoplasm, one for histidine biosynthesis in the cytoplasm, and one for phenylalanine synthesis in the periplasm.
	genomebiology.com /2001/2/8/research/0030 (7501 words)

	Porin
		The exchange between periplasm and environment occurs via porins, which may be unspecific or specific for groups of substances.
		As in LamB there is a loop in ScrY that hooks on the rim of a neighboring pore lending one aromatic amino acid to the slide and tightening the contacts in the trimer.
		On the periplasmic face another connecting device is found: each monomer contains two phenylalanines parallel to each other (stacking of pi-electrons of the aromatic rings).
	www.biologie.uni-hamburg.de /lehre/bza/kanal/porin/eporin.htm (1617 words)

	Catalase-Peroxidases of Legionella pneumophila: Cloning of the katA Gene and Studies of KatA Function -- Bandyopadhyay ...
		Periplasmic KatA is one of four antioxidant enzymes known to be essential for stationary-phase survival in gram-negative species.
		Periplasmic copper-zinc superoxide dismutase of Legionella pneumophila : a role in stationary phase survival.
		The periplasmic, group III catalase of Vibrio fischeri is required for normal symbiotic competence and is induced both by oxidative stress and by approach to stationary phase.
	jb.asm.org /cgi/content/full/182/23/6679 (4259 words)

	Overexpression of Protein Disulfide Isomerase DsbC Stabilizes Multiple-Disulfide-Bonded Recombinant Protein Produced ...
		Whole-cell (W) and periplasmic (P) protein fractions were prepared and analyzed by SDS-PAGE followed by immunoblotting as described in Materials and Methods.
		Samples (100 ml) were taken at the times indicated (after HRP induction), fractionated into whole-cell (W), periplasmic (P), and spheroplast (S) fractions, and analyzed as described in Materials and Methods.
		Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin.
	aem.asm.org /cgi/content/full/66/9/3960 (3832 words)

	Microbial Cell Factories \| Full text \| Protein quality control in the bacterial periplasm
		The bacterial periplasm is separated from the extracellular milieu only by the porous outer membrane, and hence is more susceptible to changes in the environment than the cytoplasm.
		Indeed, as the periplasm lacks ATP, periplasmic chaperones must be mechanistically distinct from their cytoplasmic counterparts, most of which use ATP to drive their cycles of substrate binding and release.
		Because DegP is intimately involved in the degradation of misfolded proteins in the periplasm, it was conceivable that DegP co-aggregated with misfolded MalE31 during the formation of IBs.
	www.microbialcellfactories.com /content/3/1/4 (8344 words)

	RedNova News - Science - Structural Biology and Structure-Based Inhibitor Design of Cholera Toxin and Heat-Labile ... (Site not responding. Last check: 2007-11-06)
		Then both the A and B subunits of the toxin are translocated across the inner membrane into the periplasm of the respective bacterium, where one A subunit and five B subunits assemble into one hetero-hexameric holotoxin.
		The CT and LT holotoxins are assembled in the periplasm of V. cholcrae and ETEC by pentamerizalion of B subunits around the C- terminal tail of the A2 domain of the A subunit, although B pentamers can form in the absence of A subunit.
		After assembly of the holotoxin in the periplasm, the toxin is translocated in the folded state across the outer membrane by the Eps export apparatus of proteins in V. cholerae (Sandkvist et al, 2000; Sandkvist, 2001) and the recently discovered, closely related Gsp assembly of proteins in ETEC (Tauschek et al., 2002).
	www.rednova.com /news/display/?id=104182 (3446 words)

	Welcome to Adobe GoLive 4 (Site not responding. Last check: 2007-11-06)
		coli expression, is deficient in two proteases encoded by the lon (cytoplasmic) and omp T (periplasmic) genes.
		In the periplasm proteolytic degradartion of proteins is decreased.
		This is mainly because the total number of proteins in the periplasm is lower.
	www.embl-heidelberg.de /ExternalInfo/geerlof/draft_frames/flowchart/exp_e_coli/stability.html (225 words)

	Crystal structure of a defective folding protein -- Saul et al. 12 (3): 577 -- Protein Science
		Periplasmic (soluble) and membrane (insoluble) fractions, corresponding to 5
		Arie, J.-P., Sassoon, N., and Betton, J.-M. Chaperone function of FkpA, a heat shock prolyl isomerase, in the periplasm of Escherichia coli.
		Betton, J.-M., Sassoon, N., Hofnung, M., and Laurent, M. Degradation versus aggregation of misfolded maltose-binding protein in the periplasm of Escherichia coli.
	www.proteinscience.org /cgi/content/full/12/3/577 (5001 words)

	The efficient export of NADP-containing glucose-fructose oxidoreductase to the periplasm of Zymomonas mobilis depends ... (Site not responding. Last check: 2007-11-06)
		The efficient export of NADP-containing glucose-fructose oxidoreductase to the periplasm of Zymomonas mobilis depends both on an intact twin-arginine motif in the signal peptide and on the generation of a structural export signal induced by cofactor binding -- Halbig et al.
		The efficient export of NADP-containing glucose-fructose oxidoreductase to the periplasm of Zymomonas mobilis depends both on an intact twin-arginine motif in the signal peptide and on the generation of a structural export signal induced by cofactor binding
		with subsequent periplasmic assembly to the holoenzyme or translocation
	www.ejbiochem.org /cgi/content/full/263/2/543 (6780 words)

	Complementation of DsbA deficiency with secreted thioredoxin variants reveals the crucial role of an efficient dithiol ... (Site not responding. Last check: 2007-11-06)
		Transport to the periplasm of thioredoxin and levels of the periplasmically expressed thioredoxin variants, the PDI a-domain and wild-type DsbA.
		Periplasmic extracts of cells grown at 37°C to the mid-log phase in DYT medium are shown.
		Rietsch, A., Bessette, P., Georgiou, G. and Beckwith, J. Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin.
	embojournal.npgjournals.com /cgi/content/full/18/12/3271 (7523 words)

	Morphogenesis of Escherichia coli -- Nanninga 62 (1): 110 -- Microbiology and Molecular Biology Reviews
		Cell shape and the interaction of cytoplasm and periplasm.
		So far, we have concentrated on the periplasmic aspects of cell division, namely, the ingrowth of the peptidoglycan layer.
		Note that the structure unites the cytoplasm and periplasm.
	mmbr.asm.org /cgi/content/full/62/1/110 (9341 words)

	Laboratory Satish Raina (Site not responding. Last check: 2007-11-06)
		To study protein folding in the bacterial periplasm, we utilized multiple genetic and biochemical approaches.
		In a second approach, we investigated the possibility of a cellular response to the accumulation of misfolded proteins in the periplasm.
		Probing the structural role of an ab loop of maltose-binding protein by mutagenesis: Heat-shock induction by loop variants of the maltose-binding protein that form periplasmic inclusion bodies.
	facmed.unige.ch /BIOMED/raina.html (1145 words)

	The Export of Coat Protein from Enteroaggregative Escherichia coli by a Specific ATP-binding Cassette Transporter ...
		B, immunoblot to detect Aap in periplasmic fractions of bacteria examined in panel A., immunoblot to detect the AatA protein in whole cell extracts of cultures examined in panel A.
		coli heat-stable toxins) from the periplasm ( 48).
		The dispersin protein is secreted into the periplasm in an Aat-independent fashion, most likely via the Sec system; a typical signal sequence is removed in this process.
	www.jbc.org /cgi/content/full/278/46/45680 (6437 words)

	UCSF - Mission Bay - Spotlighting Science
		As a result, part of the porin’s molecular structure, the C terminus, remains exposed in the periplasm when normally it would be hidden, and it binds to a periplasmic protease called DegS.
		Transcription of the genome accelerates, and soon the cell is making proteins with a variety of functions related to defense of the periplasm: fortifying the outer membrane with lipids, for instance, and marshaling more porins toward the surface.
		The cellular protease DegS, for instance, is held inactive in the bacterium’s periplasm by a part of its own molecular structure called the PDZ domain.
	pub.ucsf.edu /missionbay/science/gross.php (936 words)

	Localization and function of the IdiA homologue Slr1295 in the cyanobacterium Synechocystis sp. strain PCC 6803 -- ...
		A, Total cell-free extract; B, soluble protein fraction of the periplasm; C, total cell-free extract of spheroplast fraction after French press treatment; D, soluble protein fraction of spheroplast preparation; E, total membrane fraction of spheroplast preparation; F, thylakoid membranes; G, cytoplasmic membranes.
		Twenty-five micrograms protein (except for periplasm fraction, 15 µg protein) was applied in each lane.
		Slr0513, transport into the periplasm and the lumen is indicated.
	mic.sgmjournals.org /cgi/content/full/148/10/3293 (6065 words)

	Protein quality control in the bacterial periplasm \| Microbial Cell Factories, Volume 3 - Mirror @ Uni Potsdam (Site not responding. Last check: 2007-11-06)
		In coli, exported proteins with an ultimate destination of the periplasm and outer membrane are synthesized as precursors with a cleavable amino-terminal signal sequence.
		Once they reach the periplasm and after signal sequence cleavage, periplasmic proteins may encounter two types of protein folding catalysts: protein disulphide isomerases (Dsb proteins), which catalyse disulfide-bond formation, and peptidyl-prolyl isomerases (PPIase), which catalyse cis-trans isomerization of peptidyl bonds.
		An alternative fate for misfolded periplasmic proteins is degradation by proteases (Figure 1).
	bmc.ub.uni-potsdam.de /cgi-bin/show.pl?1475-2859-3-4 (8717 words)

	Structure and Assembly of beta -Barrel Membrane Proteins -- Tamm et al. 276 (35): 32399 -- Journal of Biological ...
		periplasmic space, the signal sequence is removed by a signal
		Various folding catalysts are known to exist in the periplasm of Gram-negative bacteria.
		The eight transmembrane strands are connected with tight turns on the periplasmic side and long flexible loops on the outer surface.
	www.jbc.org /cgi/content/full/276/35/32399 (3277 words)

	BioMed Central \| Full text \| Recognition of secretory proteins in Escherichia coli requires signals in addition to the ...
		That is, proteins that are supposed to remain in the cytoplasm are not mislocalized to the periplasm.
		To ensure that all periplasmic contents were released during the spheroplast preparation, the blots were stripped of FLAG antibody and reprobed with antibody directed against MalE, a periplasmic binding protein.
		All detectable MalE was present in the periplasmic fractions (data not shown), demonstrating that complete release of the periplasmic contents had been attained.
	www.biomedcentral.com /1471-2180/2/32 (4200 words)

	articles1 (Site not responding. Last check: 2007-11-06)
		All bacteria use chemiosmotic mechanisms to harness energy; therefore the periplasm bounded by the cell wall is an essential part of prokaryotic cells.
		Following the establishment of mitochondria, the original plasma membrane-bound metabolism of prokaryotes, as well as the funcion of the periplasm providing a compartment for the formation of different ion gradients, has been transferred into the inner mitochondrial membrane and intermembrane space.
		After the loss of the essential function of periplasm, the bacterial cell wall could also be lost, which enabled the naked cells to establish direct connections among themselves.
	www.metu.edu.tr /~bilgincc/articles.htm (2018 words)

	BIOL 400/401 Lecture 13 Summary (Site not responding. Last check: 2007-11-06)
		The complex grabs this proton, and another proton from water in the cytoplasm (fl arrows), but does not retain them, since the iron atoms at the center of Complex I are stable even with the 2 negatively-charged electrons.
		When the 2 molecules of Coenzyme Q lose their electrons they are each left with a charge of +2, which they neutralize by losing 2 protons each (fl arrows).
		Since no protons are pumped into the periplasm by Complex II, however, the total number of protons pushed out is 6 (4 from Complex III and 2 from Complex IV).
	www.ku.edu /~micro400/lectures/lecture13/lect13.htm (1851 words)

	Research Groups - Dept. of Biochemistry, Oxford (Site not responding. Last check: 2007-11-06)
		Both this enzyme and some of the proteins, eg cytochrome c, that transfer electrons to it from the respiratory chain are water-soluble and located in the periplasm, the region of the Gram negative cell that lies between the cytoplasmic membrane and the cell wall.
		The polypeptide and the haem are both first made in the cytoplasm and, it is currently believed, translocated separately to the periplasm.
		A fascinating aspect is that the assembly process has recently been found to be interrelated with the functioning of the apparatus that makes disulphide bonds in the bacterial periplasm.
	www.bioch.ox.ac.uk /aspsite/research/rgroupsnew.asp?Group_ID=22 (551 words)

Try your search on: Qwika (all wikis)