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Topic: Periplasmatic space

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	DG3: DBA case T 0158/91 - 3.3.2
		Sufficient guidance was given in the specification to enable the skilled man to extend the special teaching to the whole of the field claimed, in particular to human growth hormone, by using routine methods of experimentation with a reasonable expectation of success.
		Rather the pre- fibroplast interferon, described in document (2) was indeed known to be quite unstable and thus it was not surprising for the skilled person that the hyperlabile protein could not be identified in the periplasm.
		On page 1 of the description of the published application in the paragraph "field of the invention" it is said that the invention of document (7) generally relates to a method of synthesising within a bacterial host, and secreting through the membrane of the host, a selected protein or polypeptide.
	legal.european-patent-office.org /dg3/biblio/t910158eu1.htm (2498 words)

	Gold Gram (Site not responding. Last check: 2007-10-12)
		By converting a string to N-grams, it can be embedded in a vector space thus allowing the string to be compared to other strings in an efficient manner.
		For example, if we convert strings with only letters in the English alphabet into 3-grams, we get a 26³ dimensional space (the first dimension measures the number of occurrences of "aaa", the second "aab", and so forth for all possible combinations of three letters).
		The decolorizing mixture causes dehydration of the multilayered peptidoglycan in the Gram-positive cell wall, thus decreasing the space between the molecules and causing the cell wall to trap the crystal violet-iodine complex within the cell.
	www.wwwtln.com /finance/87/gold-gram.html (1853 words)

	The influence of R-plasmid on the Structure of Membrans of Salmanella derby Сells
		The reason of blurring small-angle reflexes can become proteins in membrane periplasmatic space, about which there are no dates in the literature.
		From obtained results follows that the midline distances /d/ of small-angle reflexes for plasmid carrying cell membranes are a little less, then the same of plasmid-free cell membranes.
		In this case at increase of concentration of water it completely accumulated in intermembrane space bringing to linear increasing of intermembrane distance.
	acadjournal.com /2001/V5/part2/p2 (1989 words)

	DG3: DBA case T 0060/89 - 3.3.2
		There was no hint whatsoever in this document to fuse DNA coding for a heterologous protein to bacterial DNA coding for the leader sequence of a bacterial protein which was to be transported through the cell membrane into either the periplasmatic space or the extracellular medium.
		The meaning of "a" membrane is supported by the whole content of the patent specification which does not refer solely to a certain inner membrane or outer membrane of a certain bacteria but rather to both kinds of membranes, i.e.
		excretion into the periplasmatic space and the extracellular medium is described; further, not only a particular bacterial strain can be used according to the description but rather strains which are likewise suitable.
	legal.european-patent-office.org /dg3/biblio/t890060ep1.htm (3759 words)

	Patent 6,506,582
		For this a fusion product is preferably used which is composed of the signal sequence that is suitable for secretion of proteins in the host organisms used and of the nucleic acid that codes for the protein.
		In this case the protein is either secreted into the medium (in gram-positive bacteria) or into the periplasmatic space (in gram-negative bacteria).
		It is expedient to insert a cleavage site between the signal sequence and the sequence coding for IL-16 which allows cleavage of the protein either during processing or in an additional step.
	www.pharmcast.com /Patents/Yr2003/Jan2003/011403/6506582_IL16011403.htm (3012 words)

	Department of Biotechnology, Chemistry and Environmental Engineering Membrane Protein group
		We are therefore engaged in an extensive analysis of the in vitro membrane protein folding process using several model systems, all from E. coli:
		The monomer DsbB (disulfide-bond forming protein B) helps form disulfide bridges in the periplasmatic space.
		It is a 176-amino acid membrane protein with 4 (predicted) transmembrane helices which integrate the protein within the inner membrane.
	www.bio.auc.dk /en/biotechnology/research_groups/biophysics (787 words)

	Chapter Abstracts: Brucella: Molecular and Cellular Biology
		-1,2-glucans are unique periplasmatic polysaccharides produced by Brucella, Rhizobium, and Agrobacterium, bacteria that belong to the Proteobacteria group and display intimate interactions with plant or animal eukaryotic cells.
		Under low osmolarity conditions cyclic glucans are highly accumulated in the periplasmatic space reaching values ranging from 5 to 20 % of the total cellular dry weight, thus suggesting that they may play a role in adaptation and/or protection of the bacterial cells under certain environmental conditions.
		Moreover ndvA, chvA and cgt null mutants, unable to transport cyclic glucan into the periplasm, display defective host interaction, thus suggesting that secretion into the periplasmic space is required to exert its action.
	www.horizonpress.com /hsp/abs/absbruc.html (3833 words)

	Gram-negative Bacteria
		The difference lies in the cell wallof the two types of bacteria; in contrast to most Gram-positivebacteria, Gram-negative bacteria have only a few layers of peptidoglycanand a secondary cell membrane made primarily of lipopolysaccharide.
		The space between the layers of peptidoglycan and the secondary cell membrane is called periplasmatic space.
		Both Gram-positive and Gram-negative bacteria may have a membrane called an S-layer.
	www.lumrix.com /medical/bacteria/gram-negative.html (512 words)

	Molecular Cloning and Characterization of cgt, the Brucella abortus Cyclic {beta}-1,2-Glucan Transporter Gene, and Its ...
		glucan is located in the periplasmic space (7), the protein
		of cyclic glucan in the periplasmic space is required in B.
		the presence of the glucan in the periplasmic space stabilizes
	iai.asm.org /cgi/content/full/72/4/2263 (4090 words)

	SBNet Conference 2004 - Conference posters 31 to 40 (Site not responding. Last check: 2007-10-12)
		Studies have shown that disulfide bond formation in protein folding is a catalyzed process, and is linked to cellular metabolism.
		It is a 176-amino acid protein with 4 (predicted) transmembrane helices which integrate the protein within the inner membrane.
		Two loops extend into the periplasmatic space and contain 4 Cys residues which oxidize the periplasmic protein DsbA.
	xray.bmc.uu.se /sbnet/conf04/posters_031_040.html (1901 words)

	Charge Recombination and Protein Dynamics in Bacterial Photosynthetic Reaction Centers Entrapped in a Sol-Gel Matrix -- ...
		dimer located close to the periplasmatic side of the membrane,
		FIGURE 3 Contour plots of the distributions of rate coefficients, f(log k), between 10 and 300 K with linear spacing of contour lines.
		All plots were scaled identically to illustrate the differences between samples and illumination histories.
	www.biophysj.org /cgi/content/full/85/3/1851 (6291 words)

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