| | Current Industrial Practices of Assessing Permeability and P-Glycoprotein Interaction |
 | | The permeability studies were initiated by adding an appropriate volume of buffer containing test compound to either the apical (for apical to basolateral transport; A to B) or basolateral (for basolateral to apical transport; B to A) side of the monolayer. |
 | | Permeability is calculated based on several factors: the amount of drugs that appeared in the receiver compartment, the initial concentration in the donor compartment, and the surface area of the physical barrier (eg, lipid bilayers and cell monolayer). |
 | | The bidirectional permeability assay, where the basolateral to apical (secretory direction, B to A) permeability is compared with the apical to basolateral (absorptive direction, A to B) permeability, is regarded as the gold standard in identifying P-gp substrates because it is functionally the most direct method of measuring efflux characteristics of drug candidates. |
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