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Topic: Phosphoprotein

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	Methyl Green (Site not responding. Last check: 2007-10-01)
		The Pro-Q Diamond phosphoprotein gel stain is a fluorescent stain that selectively detects phosphoproteins; radioisotopes are not used and therefore no radioactive waste is generated.
		The Pro-Q Diamond phosphoprotein gel stain allows the detection of 1–16 ng of phosphoprotein per band, depending on the phosphorylation state of the protein.
		The Pro-Q Diamond phosphoprotein gel stain is supplied ready-to-use in three different sizes: a 200 mL size (P-33301) suitable for staining approximately four minigels; a 1 L size (P-33300) suitable for staining approximately 20 minigels or two large-format gels, e.g., 2-D gels; and a 5 L bulk-packaging size (P-33302).
	www.altcorp.com /AffinityLabeling/methylgreen.htm (837 words)

	Clontechniques APR 2003 -BD(TM) Phosphoprotein Enrichment Kit (Site not responding. Last check: 2007-10-01)
		Each Phosphoprotein Affinity Column has a maximum binding capacity of approximately 4 mg of phosphorylated protein, and the procedure is non-denaturing, so phosphoproteins remain folded throughout the process, even during the extraction and elution steps.
		Using a colorimetric phosphate detection method, we found the majority of the phosphoprotein in the eluate; negligible traces were detected in the wash fraction.
		Phosphoprotein Affinity Columns yield a concentrated solution of phosphoprotein that can be analyzed by several different methods.
	www.clontech.com /clontech/archive/APR03UPD/phosphoprotein.shtml (772 words)

	Neither phosphorylation nor the amino-terminal part of rabies virus phosphoprotein is required for its oligomerization ...
		Sequential phosphorylation of the phosphoprotein of vesicular stomatitis virus by cellular and viral protein kinases is essential for transcription activation.
		Gao, Y., Greenfield, N. J., Cleverley, D. and Lenard, J. The transcriptional form of the phosphoprotein of vesicular stomatitis virus is a trimer: structure and stability.
		Gupta, A. K., Blondel, D., Choudhary, S. and Banerjee, A. The phosphoprotein of rabies virus is phosphorylated by a unique cellular protein kinase and specific isomers of protein kinase C. Journal of Virology 74, 91-98.
	vir.sgmjournals.org /cgi/content/full/81/7/1757 (2999 words)

	Invitrogen - Molecular Probes - Section 9.4 - Multiplexed Proteomics Technology for Detecting Specific Proteins in Gels ...
		The Pro-Q Diamond phosphoprotein gel stain (Table 9.6) is supplied ready-to-use in three different sizes: a 200 mL size (P33301) suitable for staining approximately four minigels; a 1 L size (P33300) suitable for staining approximately 20 minigels or two large-format gels, e.g., 2-D gels; and a 5 L bulk-packaging size (P33302).
		The limits of detection for the stain on PVDF membrane blots are typically 8–16 ng of phosphoprotein, with a linear dynamic range of approximately 15-fold.
		This phosphoprotein blot stain may be used in conjunction with the SYPRO Ruby protein blot stain, a total-protein stain that is quantitative over two orders of magnitude on blots.
	probes.invitrogen.com /handbook/sections/0904.html (8282 words)

	Pro-Q® Diamond Phosphoprotein Gel Staning Kit includes 1L staind and 40 µl** standard from Molecular Probes ...
		Molecular Probes Pro-Q® Diamond phosphoprotein gel stain is a breakthrough technology that provides a method for selectively staining phosphoproteins in polyacrylamide gels.It is ideal for identification of kinase targets in signal transduction pathways and for phosphoproteomic studies.
		Pro-Q Diamond phosphoprotein gel stain allows detection of as little as 1 16 ng of phosphoprotein per band, depending the phosphorylation state of the protein.
		For individual phosphoproteins, the strength of the signal correlates with the number phosphate groups and is linear over three orders of magnitude.
	www.biocompare.com /itemdetails.asp?itemid=382867 (399 words)

	Research
		The phosphoprotein forms an oligomer and is constitutively phosphorylated.
		Elucidating the structure of the phosphoprotein and understanding its biochemistry more thoroughly will advance the understanding of how the phosphoprotein participates in viral replication and may provide a target for a potential therapeutic.
		In addition, the phosphoprotein is known to interact with the RSV nucleocapsid protein, promoting binding to the viral RNA.
	hogle.med.harvard.edu /html/research/rsv/rsv.html (158 words)

	Phosphoprotein with Phosphoglycerate Mutase Activity from the Archaeon Sulfolobus solfataricus -- Potters et al. 185 ...
		Phosphoprotein with Phosphoglycerate Mutase Activity from the Archaeon Sulfolobus solfataricus -- Potters et al.
		The eluted phosphoprotein was dialyzed versus 25 mM
		Archaeal phosphoproteins: identification of a hexosephosphate mutase and the
	jb.asm.org /cgi/content/full/185/7/2112 (5177 words)

	worm_ppbd_112399
		Phosphoprotein BD Phosphoprotein BD Phosphoprotein BD Phosphoprotein BD Sequence
		Phosphoprotein BD Phosphoprotein BD T08D2.7; CAB07420; Y60A3.ctg05073; T08D2.J
		Phosphoprotein BD Phosphoprotein BD 1 kinesin, 1 PH, 3 Coiled coil
	www.kinase.com /celegans/Worm_ppbd_112399.htm (90 words)

	Borna Disease Virus Phosphoprotein Binds a Neurite Outgrowth Factor, Amphoterin/HMG-1 -- Kamitani et al. 75 (18): 8742 ...
		The Borna disease virus (BDV) p24 phosphoprotein is an abundant protein in BDV-infected cultured cells and animal brains.
		is a nucleus-associated phosphoprotein and is assumed to be a
		The p24 phosphoprotein of BDV is an abundant protein in infected cultured cells and in infected animal brains (2, 3).
	jvi.asm.org /cgi/content/full/75/18/8742 (6564 words)

	Invitrogen - Molecular Probes - Tools for Phosphoprotein and Phosphopeptide Analysis
		Pro-Q Diamond phosphoprotein stains are ideal for analysis of phosphorylation of a single protein or an entire proteome.
		In this section of the gel, the computer-generated overlay of the two staining patterns reveals the phosphoproteins (magenta), and nonphosphorylated proteins (green).
		These kits are ideal for measuring phosphatase and kinase activity and for monitoring relative phosphoprotein or phosphopeptide concentrations during chromatography or after IEF fractionation of protein samples.
	probes.invitrogen.com /products/phosphoproteomics (622 words)

	Human protein: O43423 - Acidic leucine-rich nuclear phosphoprotein 32 family member C (Phosphoprotein 32-related ...
		Phosphoprotein 32 (PP32) is a tumor suppressor that can inhibit several types of cancers, including prostate and breast cancers.
		The protein encoded by this gene is one of at least two proteins that are similar in amino acid sequence to PP32 and are part of the same acidic nuclear phosphoprotein gene family.
		The tumor suppressor function of PP32 has been localized to a 25 amino acid region that is divergent between PP32 and the protein encoded by this gene.
	harvester.embl.de /harvester/O434/O43423.htm (887 words)

	Breaking News: Cell Signaling Technology Expands Partnership with Bio-Rad to Develop Phosphoprotein Assays for Kinase ...
		Under the new agreement, CST will develop antibodies for kinase activation and protein phosphorylation assays that will be commercialized in Bio-Rad's multiplex bead-based assays for the Bio-Plex(R) suspension array system.
		Bio-Plex(R) phosphoprotein assays are optimized for simultaneous assessment of kinase and signaling protein activation states and provide critical tools for disease research and drug development.
		Given the complexity of cellular signaling and the diversity of mechanistic abnormalities underlying disease, Bio-Plex(R) multiplexed phosphoprotein assays can facilitate the understanding of disease and the development of more effective targeted therapeutic strategies.
	www.genengnews.com /news/bnitem.aspx?name=2236023 (505 words)

	Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation ...
		Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P -- Poisson et al.
		Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P
		The lyssavirus phosphoprotein P is a co-factor of the viral
	vir.sgmjournals.org /cgi/content/full/82/11/2691 (3183 words)

	Dentin Phosphoprotein and Dentin Sialoprotein Are Cleavage Products Expressed from a Single Transcript Coded by a Gene ...
		Dentin Phosphoprotein and Dentin Sialoprotein Are Cleavage Products Expressed from a Single Transcript Coded by a Gene on Human Chromosome 4.
		sequences with homology to the dentin phosphoprotein oligonucleotides
		Studies in rat have suggested that the DECM may contain multiple forms of the DPPs (20, 21).
	www.jbc.org /cgi/content/full/272/2/835 (6316 words)

	InterPro: IPR003603 U2A'/phosphoprotein 32 family A, C-terminal
		Examples from the metazoa are described as either "Acidic leucine-rich nuclear phosphoprotein 32 family member A" or have been characterised as U2A', the protein that interacts with U2B'' facilitating the interaction with U2 snRNA.
		U2A' is required for the spliceosome assembly and the efficient addition of U2 snRNP onto the pre-mRNA [ 1 ].
		The crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA has been described [ 2 ].
	www.ebi.ac.uk /interpro/IEntry?ac=IPR003603 (3026 words)

	Expression of vasodilator-stimulated phosphoprotein in human placenta: possible implications in trophoblast invasion -- ...
		Expression of vasodilator-stimulated phosphoprotein in human placenta: possible implications in trophoblast invasion -- Kayisli et al.
		Immunostaining of VASP was seen in the extravillous cytotrophoblasts of the cell column in increasing intensity from proximal (PCT) to distal (DCT) extravillous cells.
		Immunoblot analysis using mouse monoclonal anti-vasodilator-stimulated phosphoprotein (VASP) antibody and densitometric quantification of the regulation of VASP expression by leukaemia inhibitory factor (LIF) and transforming growth factor (TGF)-ß1 in first trimester placenta.
	molehr.oxfordjournals.org /cgi/content/full/8/1/88 (3477 words)

	Vasodilator-Stimulated Phosphoprotein Is Involved in Stress-Fiber and Membrane Ruffle Formation in Endothelial Cells -- ...
		Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro.
		Phosphorylation of vasodilator-stimulated phosphoprotein: a consequence of nitric oxide- and cGMP-mediated signal transduction in brain capillary endothelial cells and astrocytes.
		Characterization of the actin binding properties of the vasodilator-stimulated phosphoprotein VASP.
	atvb.ahajournals.org /cgi/content/full/20/9/2051 (3939 words)

	InterPro: IPR006760 cAMP-regulated phosphoprotein/endosulfine conserved region
		This is a conserved region found in both cAMP-regulated phosphoprotein 19 (ARPP-19) and alpha/beta endosulphine.
		Dulubova I. Horiuchi A. Snyder G.L. Girault J.A. ernik A.J. Shao L. Ramabhadran R. Greengard P. Nairn A.C. ARPP-16/ARPP-19: a highly conserved family of cAMP-regulated phosphoproteins.
		Bataille D. Heron L. Virsolvy A. Peyrollier K. Lecam A. Gros L. Blache P. alpha-Endosulfine, a new entity in the control of insulin secretion.
	www.ebi.ac.uk /interpro/IEntry?ac=IPR006760 (2862 words)

	UniProtKB/Swiss-Prot entry P50552 [VASP_HUMAN] Vasodilator-stimulated phosphoprotein
		Drees B. Friederich E. Fradelizi J. Louvard D. Beckerle M.C. Golsteyn R.M. "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins.";
		Chitaley K. Chen L. Galler A. Walter U. Daum G. Clowes A.W. "Vasodilator-stimulated phosphoprotein is a substrate for protein kinase C.";
		Wentworth J.K. Pula G. Poole A.W. "Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser 157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets.";
	www.expasy.org /uniprot/P50552 (1040 words)

	UniProtKB/Swiss-Prot entry Q14978 [NOLC1_HUMAN] Nucleolar phosphoprotein p130
		Pai C.-Y. Yeh N.-H. "Cell proliferation-dependent expression of two isoforms of the nucleolar phosphoprotein p130.";
		Chen H.-K. Yeh N.-H. "The nucleolar phosphoprotein P130 is a GTPase/ATPase with intrinsic property to form large complexes triggered by F- and Mg2+.";
		Beausoleil S.A. Jedrychowski M. Schwartz D. Elias J.E. Villen J. Li J. Cohn M.A. Cantley L.C. Gygi S.P. "Large-scale characterization of HeLa cell nuclear phosphoproteins.";
	www.expasy.org /uniprot/Q14978 (533 words)

	Human protein: Q9UD71 - Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP-32). EMBL Bioinformatics Harvester
		DOPAMINE- AND cAMP-REGULATED PHOSPHOPROTEIN, 32-KD protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)
		Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders.
		Upstream genomic sequence for Protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)
	harvester.embl.de /harvester/Q9UD/Q9UD71.htm (848 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
		Energy Citations Database (ECD) Document #5933374 - Phosphoprotein phosphatase of bovine spleen cell nuclei: physicochemical properties
		Availability information may be found in the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or via the "Full-text Availability" link.
		Phosphoprotein phosphatase of bovine spleen cell nuclei: physicochemical properties
	www.osti.gov /energycitations/product.biblio.jsp?osti_id=5933374 (168 words)

	Ribosomal Phosphoprotein P0
		Large lot sizes allow lot reservations and minimize costs of assay development, view material control and kit production
		Human ribosomal phosphoprotein P0 protein (belongs to a group of antigens also called acidic ribosomal phosphoproteins or ribosomal P-proteins).
		Full-length cDNA coding for the human ribosomal P0 phosphoprotein fused to a hexa-histidine purification tag.
	www.rmdiagnostics.com /products/?cat_no=14100 (191 words)

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