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HMDS Techniques: Fluorescent Labels for Antibodies and Probes The phycobiliproteins are 'antenna' pigments used by some classes of plants to increase the efficiency of photosynthesis by collecting light energy at wavelengths over which chlorophyll absorbs poorly. This arrangement of phycobiliprotein antenna pigments and chlorophyll is found in the phycobilisomes and allows light energy across much of the visible spectrum to be used for photosynthesis. Phycobiliproteins are relatively large, and contain multiple chromophore prosthetic groups, which are responsible for the fluorescent properties of these proteins, and generally result in fluorochromes of very high efficiency, commonly in excess of 90% absorbed light energy transferred to chlorophyll. www.hmds.org.uk /fluorochrome.html   (1519 words)

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic ... Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity the latter consists of phycobiliproteins that are covalently of phycobiliproteins in Prochlorococcus, we sequenced the cpeB mic.sgmjournals.org /cgi/content/full/147/11/3171   (3815 words)

ACTA BIOCHIMICA et BIOPHYSICA SINICA The phycobiliprotein discs were assembled by disc-to-disc to form aggregate, which was the most stable state in the cell, and also helpful for the energy transfer. The assembly of phycobiliprotein discs into phycobilisomes is dependent upon the presence of different functional linker polypeptides, and the roles of the linker polypeptides in the aggregate formation and the fine-tuning of the absorption characteristics of phycobiliproteins have been extensively studied Since pure phycobiliproteins seem not to be capable of assembly into the structures characteristic of phycobilisomes in solution, it has been inferred that the linker-polypeptides play a key role in the phycobilisome assembly. www.abbs.info /fulltxt/eng/34010099.htm   (2285 words)

UNO Biological Sciences Phycobiliproteins are water-soluble and absorb light in the visible region of the spectrum. Phycobiliproteins range in color from yellow to red to purple to blue, depending upon which of a combination of four possible chromophores called bilins are covalently attached to these proteins. M.S. Characterization of phycobiliprotein lyase mutants in the cyanobacterium Synechocystis sp. biology.uno.edu /fac_web_pages/schlucterhtm.html   (1030 words)

Dojindo Molecular Technologies - Newsletter Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Phycobiliprotein Labeling Kits are for simple and rapid labeling of PE and APC to a target protein. Phycobiliprotein Labeling Kits have two kit types: one is the NH2 fype for labeling via amino groups of the target, and the other is the SH type for labeling via sulfhydryl groups. www.dojindo.com /whatsnew.html   (1649 words)

Functional Genomic Analysis of the HY2 Family of Ferredoxin-Dependent Bilin Reductases from Oxygenic Photosynthetic ... precursors of the chromophores of the light-harvesting phycobiliproteins of the phycobiliprotein chromophore precursors PCB and PEB. is the precursor of the chromophores of the phycobiliproteins www.plantcell.org /cgi/content/full/13/4/965   (6520 words)

Pfam 20.0 : HEAT_PBS   (Site not responding. Last check: 2007-11-02) Phycobiliproteins differ in sequence and in the nature and number of attached phycobilins to each of their subunits. It has been suggested that the similarity of NblB to the phycobiliprotein lyases is due to the ability to bind tetrapyrrole phycobilins via the common repeated motif PUBMED:9882677. Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon. pfam.wustl.edu /cgi-bin/getdesc?name=HEAT_PBS   (792 words)

New Page 88 is a phycobiliprotein isolated from Spirulina sp., a blue-green algae. Like other phycobiliproteins, C-Phycocyanin is fluorescent, with an extremely high absorbtivity, a high quantum efficiency, a large Stokes shift and excitation and emission bands at visible wavelengths. Like other phycobiliproteins, R-PE is fluorescent, with an extremely high absorbtivity, a high quantum efficiency, a large Stokes shift and excitation and emission bands at visible wavelengths. www.gentaur.com /new_page_88.htm   (449 words)

华瑞制药 >> 临床营养学术沙龙 >> 文献资料 >> 国外文献   (Site not responding. Last check: 2007-11-02) Phycobiliprotein genes of the marine photosynthetic prokaryoteProchlorococcus: evidence for rapid evolution of genetic heterogeneity. Recently, genes encoding the phycobiliproteinphycoerythrin were identified in several Prochlorococcus isolates, thusmaking it the only extant photosynthetic prokaryote to possess achlorophyll a/b antenna as well as phycobiliprotein genes. Although strains that are most closelyrelated to marine Synechococcus possess genes (cpeB, cpeA) encoding bothsubunits of phycoerythrin, a more recently evolved strain is shown to lackcpeA and to possess a degenerate form of cpeB. www.sspc.com.cn /narticle2.php?id=70678   (199 words)

Dr hab. Józef Grabowski, prof.at Poznań Technical University Grabowski J., E. Gantt and C.A. Lipschultz: "Studies on the structure of phycobilisomes: the behaviour of phycobiliprotein complexes under high hydrostatic pressure"; Proceedings of Polish - Hungarian Symposium on Biophysics of Macromolecules; Łódz (Poland), June 1979. Grabowski J. and A. Nowak: "The fluorescence of tryptophanyl residues in phycobiliproteins and their complexes: phycobilisomes"; Proceedings of Polish - Hungarian Symposium on Biophysics of Macromolecules, Łódz (Poland), June 1979. Grabowski J. and A. Nowak: "Application of Stiepanov's relation to phycobiliprotein' fluorescence"; Proceedings of IVth of Polish Biophysical Society, Kozubnik (Poland), October 1979. www.phys.put.poznan.pl /sensor/grabowski/publications.html   (1424 words)

AplA, a Member of a New Class of Phycobiliproteins Lacking a Traditional Role in Photosynthetic Light Harvesting -- ... AplA, a Member of a New Class of Phycobiliproteins Lacking a Traditional Role in Photosynthetic Light Harvesting -- Montgomery et al. Closed triangles indicate eight residues conserved in all phycobiliproteins; closed circles indicate eight residues primarily conserved in AP proteins. gene and is flanked by genes encoding regulators of phycobiliprotein jb.asm.org /cgi/content/full/186/21/7420   (4981 words)

Christa L. Colyer - Research Interests and Publications The determination of phycobiliproteins and investigations into the mechanism of noncovalent binding of nonfluorescent proteins with visible and near-infrared organic dyes are examples of current projects in the Colyer lab. Phycobiliproteins are water soluble, highly fluorescent proteins produced in nature by cyanobacteria (blue-green algae) and red algae. To facilitate phycobiliprotein determinations, we are developing high-speed and high-efficiency extraction methods, followed by high-sensitivity CE-LIF separation methods. www.wfu.edu /academics/chemistry/faculty/ChristaColyerResearch.html   (813 words)

Single-Molecule Spectroscopy Selectively Probes Donor and Acceptor Chromophores in the Phycobiliprotein Allophycocyanin ...   (Site not responding. Last check: 2007-11-02) They absorb visible light, from 450 to 650 nm and transfer the excitation energy to the reaction center where it is converted to chemical energy via fast electron transfer. Phycobilisomes consist of ordered assemblies of several phycobiliproteins, like phycoerythrins, phycoerythrocyanins, and allophycocyanins, and are organized in rod-like structures with phycoerythrins at the outer ends of the rods, phycoerythrocyanins in the middle, and allophycocyanins in the center, that is, in close proximity to the reaction center. The excitation energy collected upon the absorption of a photon is transferred to the reaction center with an efficiency approaching unity (Van Grondelle, 1985). www.findarticles.com /p/articles/mi_qa3938/is_200410/ai_n9416520   (635 words)

Invitrogen - Molecular Probes - BioProbes 30 - Tools for Flow Cytometry Our pure phycobiliproteins (Table 4) and pyridyldisulfide derivative of R-phycoerythrin (P-806) are the perfect tools for creating your own phycobiliprotein conjugates. Phycobiliproteins, including B-phycoerythrin (B-PE), R-phycoerythrin (R-PE) and allophycocyanin (APC) belong to a family of highly fluorescent proteins derived from cyanobacteria and eukaryotic algae. Molecular Probes is licensed to produce phycobiliproteins and their conjugates under patents owned by Stanford University, including U.S. Patent Nos. probes.invitrogen.com /lit/bioprobes30/section5.html   (1975 words)

The Synechococcus Strain PCC 7942 glnN Product (Glutamine Synthetase III) Helps Recovery from Prolonged Nitrogen ... The amount of phycobiliproteins was estimated as described previously (12) and shown as the relative phycocyanin content per OD (rel. -subunit phycobiliprotein lyase involved in degradation of phycobilisomes. Nitrogen starvation in Synechococcus strain PCC 7942: involvement of glutamine synthetase and NtcA in phycobiliprotein degradation and survival. jb.asm.org /cgi/content/full/182/19/5615   (2953 words)

Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations Energy Citations Database (ECD) Document #7097784 - Structure and phycobiliprotein composition of phycobilisomes from Griffithsia pacifica (Rhodophyceae) Availability information may be found in the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or via the "Full-text Availability" link. Structure and phycobiliprotein composition of phycobilisomes from Griffithsia pacifica (Rhodophyceae) www.osti.gov /energycitations/product.biblio.jsp?osti_id=7097784   (107 words)

Lagarias Lab BV reduction by one of several ferredoxin-dependent bilin reductases, HY2, PcyA, PebAB, or the NADPH-dependent BV reductase BvdR with distinct regiospecificities yield a wide variety of linear tetrapyrrole precursors of the chromophores of phycobiliproteins, phytochromes and phytochrome-related proteins. The phytobilins PΦB, PCB and PEB function as the immediate precursors of the light harvesting prosthetic groups of the plant photoreceptor phytochrome and the phycobiliprotein antennae of cyanobacteria, red algae and cryptomonads (Frankenberg, N.F., and Lagarias, J.C. Biosynthesis and biological function of bilins. The biosynthesis of these pigments is thus critical for plant photomorphogenesis and for survival of phycobiliprotein-containing organisms that live in light-limiting environments. www.mcb.ucdavis.edu /faculty-labs/lagarias/project2.html   (607 words)

Cyanotech — Phycobiliproteins Phycobiliproteins are naturally occurring, efficient light harvesting algal pigments that are highly fluorescent when purified. Each phycobiliprotein has a unique emission peak making multicolor labeling possible. CONVENIENCE: Phycobiliproteins are water-soluble and stable in solution or as solids. www.cyanotech.com /html/phy/phyco.html   (223 words)

Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-alpha subunit in a heterologous host -- Tooley et al. ... The phycobiliproteins are a family of light-harvesting proteins found in cyanobacteria, red algae, and the cryptomonads. bilin attached to the phycobiliproteins in this cyanobacterium. Phycobiliprotein subunits are highly versatile as fusion partners (11). www.pnas.org /cgi/content/full/98/19/10560   (3278 words)

P r o c h l o r o c o c c u s The DNA-sequence of this region has been analysed prior to the start of this project and is the basis for a detailed analysis of individual gene functions. Genes encoding structural phycobiliproteins are shown in green. Hess W.R., Partensky F. Analysis of a phycobiliprotein gene cluster in Prochlorococcus marinus CCMP 1375: Identification of a putative linker polypeptide and the phylogeny of Prochlorococcus phycoerythrins. www.biologie.hu-berlin.de /~genetics/prochloro.html   (623 words)

Pfam 20.0 : Fe_bilin_red   (Site not responding. Last check: 2007-11-02) Phytobilins are linear tetrapyrrole precursors of the light-harvesting prosthetic groups of the phytochrome photoreceptors of plants and the phycobiliprotein photosynthetic antennae of cyanobacteria, red algae, and cryptomonads. It is known that that phytobilins are synthesised from heme via the intermediacy of biliverdin IX alpha (BV), which is reduced subsequently by ferredoxin-dependent bilin reductases with different double-bond specificities [1]. It is known that that phytobilins are synthesised from heme via the intermediacy of biliverdin IX alpha (BV), which is reduced subsequently by ferredoxin-dependent bilin reductases with different double-bond specificities PUBMED:11283349. pfam.wustl.edu /cgi-bin/getdesc?name=Fe_bilin_red   (186 words)

Phycobiliproteins Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. Kronick, M.N. The use of phycobiliproteins as fluorescent labels in immunoassay. Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels. www.piercenet.com /Products/Browse.cfm?fldID=02040404&Format=Print   (92 words)

The Cyanobacteria: page 5 DNA is free in the cytoplasm and is not enclosed in a membrane, it is not central as in Cyanophyta but is rather diffuse throughout the cell. A new Division of prokaryotic algae was erected, and named the Prochlorophyta. Unlike the Cyanobacteria they contain the additional photosynthetic pigment, chlorophyll-b and they lack the phycobiliprotein pigments. hypnea.botany.uwc.ac.za /phylogeny/classif/cyan5.htm   (191 words)

Chemicon - Product #45-001 - Beadlyte® Streptavidin-Phycoerythrin Beadlyte® Streptavidin-Phycoerythrin is a fluorescent conjugate optimized for use in Beadlyte® Luminex based assays. R-Phycoerythrin is a phycobiliprotein isolated from red algae with a primary absorbance peak of 565nm, secondary absorbance peaks at 480 and 546nm and fluorescence emission at 578nm. The intensely fluorescent property of R-Phycoerythrin is due to a quantum efficiency in excess of 90% and multisubunit protein structure of 240 kDa with multiple chromophores covalently bound to each subunit. www.chemicon.com /browse/productdetail.asp?ProductID=45-001   (472 words)

Flogen Phycobiliprotein   (Site not responding. Last check: 2007-11-02) To strengthen our assurance to quality in our products, we have certified Good Manufacturing Practice (GMP), ISO 9001 compliance, and certified organic products. Flogen® is the brand name for the line of phycobiliprotein products. Harvest at the optimal stage of the growth cycle to assure uniform product characteristics www.febico.com.tw /Flogen/English/Introduction.htm   (106 words)

Phycocyanobilin:Ferredoxin Oxidoreductase of Anabaena sp. PCC 7120. BIOCHEMICAL AND SPECTROSCOPIC CHARACTERIZATION -- ... Received for publication, November 14, 2002, and in revised form, December 26, 2002 In cyanobacteria, the biosynthesis of the phycobiliprotein and phytochrome chromophore precursor phycocyanobilin is catalyzed direct precursor of the chromophores of the light-harvesting phycobiliproteins www.jbc.org /cgi/content/full/278/11/9219   (4201 words)

LinHomePage Heme regulates expression of phycobiliprotein photosgene in the unicellular rhodophyte C. caldarium, J. Biol. Studies on Cyanidium caldarium phycobiliprotein mutants, Plant Physiol. Green Fluorescent Protein: Properties, Applications and Protocols, Second Edition, Edited by Chalfie and Kain, Wiley Inter-science, p285-304 www.mcdb.ucla.edu /Research/ShuoLin/publications1.html   (1374 words)

BioPharmaLink profile of Hangzhou Aowei Bioengineering Co., Ltd, China, Hangzhou Learn more About us and about our Services Our main bioproducts include: fluorescence phycocyanin, phycoerythirin, phycobiliprotein, alga polysaccharide, etc. We also develop and provide products as scientific & diagnostic reagents, pharmaceutical raw materials, health care food, cosmetic materials and additives. biopharmalink.com /companies/4372.htm   (72 words)

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