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	Plant Tissue Culture
		Plant tissue culture involves the growing of plant tissue from plant material taken from a source plant.It has been found that plants can reproduce whole plants from fragments of plant material when given a nutrient media capable of supporting growth and appropriate hormone control.
		Cell division in tissue had to await the discovery of kinetin.This was first discovered by autoclaving freshly isolated slurries of DNA from Herring sperm.The discovery of cytokinins gave much impetus to tissue culture.
		Tissue culture is thus proving to be useful in a variety of ways including plant propagation,raising and maintenace of high health status plants,germ plasm storage,and a valuable technique in plant improvement..In plant improvement tissue culture maybe used in the technique of gene insertion to improve plant stocks.
	members.aol.com /MrDJReed/private/PTC.html (252 words)

	Plant tissue culture (Site not responding. Last check: 2007-10-14)
		Tissue culture is a sun-rise technology for the multiplication of superior plants on large scale on a perpetual basis, production of novel and improved plants and for biosynthesis of products of industrial and medicinal value from natural plant resources.
		Tissue culture in simple terms refers to the cultivation of the cells and tissues free from the mother plant in appropriate nutrient substrates under sterile conditions.
		Such aspects as plant tissue preparation, media preparation, culture initiation, contamination and browning, plant multiplication, haploidy in plant breeding, vectors used for plant transformation, hardening and field establishment were taught.
	www.jesuitsinscience.org /Newsletter97/ptc.htm (577 words)

	[No title] (Site not responding. Last check: 2007-10-14)
		History of plant tissue culture The history of plant tissue culture begins with the concept of the cell theory given independently by Schleiden 1838 and Schwann 1839 which implied.that the cell is a functional unit.
		New plants arising from unorganized cells or tissues to form unorganized cells and tissues Cytodifferentiation In plant tissue culture during growth and maturation of the callus tissue or free cells in suspension culture few dedifferentiated cells undergo cytosenescence and redifferentiated to vascular tissues.
		Mixed enzymatic Plant tissue are plasmolysed in the presence of a mixture of pectinase and cellulases thus inducing simultaneous separation of cells and degradation of cell wall to release protoplast directly.
	www.tnau.ac.in /notesbscag/notestry/abt401/tissue_culture.doc (5906 words)

	The Carnivorous Plant FAQ: David Purk's hints
		Tissue culture is a term with a number of definitions that varies by the user; here I will use it to mean the propagation and multiplication of plant cells in artificial conditions in sterile (or near sterile) conditions.
		Tissue culture has been with us since the late 19th century, and while it has become very technically advanced, some of the basic methods are relatively easy to maintain.
		Tissue culture practitioners first must create their media-the nutrient rich solution that will house the chunk of plant cells that will be encouraged to reproduce and grow.
	www.sarracenia.com /faq/faq6010i.html (3315 words)

	Tissue culture Summary
		Tissue culture techniques are also used to generate large numbers of genetically identical plants for agricultural applications, or to generate additional plants of rare or endangered species.
		Tissue culture is defined as the propagation of plant and animal cells through the placement of small amounts of tissue in an artificial environment.
		A tissue culture is the growth of cells (tissue) separate from the organism.
	www.bookrags.com /Tissue_culture (1265 words)

	The Many Dimensions of Plant Tissue Culture Research
		The major impact of plant tissue culture will not be felt in the area of micropropagation, however, but in the area of controlled manipulations of plants at the cellular level in ways which have not been possible prior to the introduction of tissue culture techniques.
		Plant tissues known to be free of the pathogen under consideration (viral, bacterial, or fungal) are physically selected as the explant for tissue culture.
		The ability to fuse plant cells from species which may be incompatible as sexual crosses and the ability of plant cells to take up and incorporate foreign genetic codes extend the realm of plant modifications through tissue culture to the limits of the imagination.
	aggie-horticulture.tamu.edu /tisscult/pltissue/pltissue.html (1693 words)

	Plant Tissue Culture
		Plant Tissue Culture, more technically known as micropropagation, can be broadly defined as a collection of methods used to grow large numbers of plant cells, in vitro, in an aseptic and closely controlled environment.
		The medium used to grow the plant tissue, the plant tissues themselves, and the environment surrounding the tissue culture, must be free of all possible contaminants.
		Plant tissue culture is also advantageous to growers because the overwhelming number of plants can be produced using the tissue collected from a single parent plant – a plant which itself remains unharmed in the tissue harvesting process.
	www.bio.davidson.edu /people/kabernd/seminar/2002/method/amy/aj.htm (648 words)

	Phytocultures – Goof Proof Plants, Orchids and Florababies – Tissue Culture - Canada
		Plant tissue culture processes were initially developed and practiced in university and government-based laboratories.
		Plant tissue culture has revolutionized the flower and nursery markets by making valuable new hybrid clones available in commercial quantities comparatively soon after their first discovery.
		Plants produced by vegetative propagation including top cutting, root divisions, pseudobulbs, offshoots and plantlets, are genetically identical to the mother plant and thus members of a single clone.
	www.phytocultures.com /tissue_procedure.asp (524 words)

	Plant tissue culture - Wikipedia, the free encyclopedia
		Plant tissue culture, also called micropropagation, is a practice used to propagate plants under sterile conditions, often to produce clones of a plant.
		Single cells, plant cells without cell wall(protoplasts), pieces of leaves, or (less commonly) roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones.
		Modern plant tissue culture is performed under aseptic conditions under filtered air.
	en.wikipedia.org /wiki/Plant_tissue_culture (849 words)

	Phytocultures – Goof Proof Plants, Orchids and Florababies – Tissue Culture - Canada
		Plant tissue culture is a specialized technology used for plant propagation.
		We designate these plants to be the mother plants or the donor of plant tissues to start the tissue culture process.
		Bacteria and fungi are excluded from the mother plant, it is also necessary to exclude these organisms from the growth of the plant tissue therefore, all manipulations are carried out under sterile conditions.
	www.phytocultures.com /tissue_whatisit.asp (263 words)

	CULTURING PLANTS FROM EMBRYONIC PLANT TISSUE
		Plant embryos or pieces of embryonic tissue are readily obtainable from seeds that have been soaked.
		The purpose of this lab is to grow plants in tissue cultures and to see what effect the different concentrations of hormones have on embryo development.
		When doing tissue cultures, the work area and equipment should be kept as sterile as possible to prevent contamination from molds or other microorganisms.
	www.accessexcellence.org /AE/AEPC/WWC/1993/culturing.html (1796 words)

	Plant issue culture (Site not responding. Last check: 2007-10-14)
		In tissue culture cells, tissues, and organs of a plant are separated.
		The cultured plant requires a source of energy from sugar, salts, a few vitamins, amino acids, etc. that are provided in the nutrient media.
		When the apex of shoot is used for multiplication by tissue culture, we get disease free plants because the shoot apical meristem, a group of dividing cells at the tip of a stem or root, is free from pathogens.
	edugreen.teri.res.in /explore/bio/tissue.htm (850 words)

	LifeGuard plant tissue culture
		culture vessels have been developed for plant culture by tissue culture professionals, and are proven in use in both academic and commercial plant tissue culture laboratories around the world.
		The choice of which to use depends on the size of the plants and the amount of agar or liquid medium to be used over the growth period.
		Jackson, Abbott and Belcher, Ventilation in plant tissue cultures and effects of poor aeration, Ann.
	www.osmotek.com /LifeGuard.html (1268 words)

	Plant Tissue Culture (Site not responding. Last check: 2007-10-14)
		Plant tissue culture is a form of biotechnology with great potential to aid agriculture in developing countries.
		The great value of plant tissue culture is that it allows the developmental fate of the plant tissues to be manipulated toward desired ends by inclusion of growth regulators (plant hormones) and other chemicals in the medium.
		The ability to culture plant tissues forms the basis for several biotechnologies and is a central component of crop genetic engineering programs.
	www.artsci.wustl.edu /~anthro/research/enhance/tissue1.html (386 words)

	Plant Tissue Culture - Online
		The course will be based on the CD, "Plant Tissue Culture for the Classroom and Home" but the CD does not need to be purchased to take the course.
		We plan to have online chats with guests who are active in tissue culture and/or have experience in various areas and can answer questions about techniques, specific species, or the industry in general.
		This course is designed to meet the educational needs of the hobbyist, student, teacher (who wants to add new technology to their classroom) and the commercial grower (who wants to determine if tissue culture propagation techniques might be useful in their business).
	www.kitchenculturekit.com /onlineinfo.htm (605 words)

	Preparing Plant Tissue Culture Medium
		In the plant tissue culture process, plant parts are cut into small pieces that will give rise to many more individual plants.
		Plant tissue cultures need an outside source of sucrose because the tissue culture cells go into shock when the explants are made.
		Plant tissue culture medium MUST be sterilized since the cultures will be in progress for much more than 8 weeks.
	carnegieinstitution.org /first_light_case/horn/PTC/preptcmed.html (1321 words)

	BRWM: MissVitro Plant Tissue Culture Laboratory (Site not responding. Last check: 2007-10-14)
		Although some hostas have proven to be difficult to tissue culture due to complex variegation patterns, such as the streaked varieties, most plants "come true" in the TC Laboratory, including many of the medio variegated varieties, and virtually all of the solid colored ones.
		Plants are shipped only after they have become established in a soil-less mix.
		If the plant you send us for TC is your hybrid or mutation and not yet in the trade you retain all rights to all plants produced, for an agreed upon period of time, or indefinitely if you wish.
	www.brwm.org /missvitroplanttissueculturelaboratory (280 words)

	Tissue culture - Wikipedia, the free encyclopedia
		Tissue culture refers to the growth of tissues and/or cells separate from the organism.
		In modern usage, "tissue culture" often refers to the growth of animal or plant cells in vitro.
		In particular, the term is often used interchangeably with cell culture to specifically describe the in vitro culturing of mammalian cells.
	en.wikipedia.org /wiki/Tissue_culture (155 words)

	NMIT - Plant Tissue Culture
		Students are encouraged to work on plant material with which they have a particular interest.
		Staff of plant nurseries will also be trained to deflask tissue-cultured plants obtained from tissue culture laboratories.
		Performing aseptic techniques and surface sterilisation of plant materials, culture, media preparation, transfer of cultures and deflasking in the tissue culture laboratory and glasshouse.
	www.nmit.vic.edu.au /courses/horticulture/plant_tissue_a.html (415 words)

	Carnivorous Plant Tissue Culture - Media, Techniques & sterilization
		From this point on it will be assumed you have purchased a tissue culture kit from somewhere and have a basic idea of what's going on.
		On the links page you will find a link to Kitchen Culture Kits which is where I got my carnivorous plant tissue culture kit from.
		As it is assumed you have received a reputable carnivorous plant tissue culture kit it is also assumed you have autoclave lids for baby food jars and quart/pint jars and know how to mix/sterilize media & carnivorous plant parts.
	www.world-of-carnivores.com /tissue_culture.html (466 words)

	Plant tissue culture (Site not responding. Last check: 2007-10-14)
		Our tissue culture lab has greatly enhanced our breeding programs enabling us to bring to market new varieties quicker.
		Stage III tissue culture (in-vitro plantlets with roots) are packed 40 per tub, 320 per box (minimum).
		Stage IV tissue culture (in-vitro plantlets planted into a 105 cell tray, rooted for 4-6 weeks) minimum per variety 105, minimum shipment of 6 - 105 trays.
	www.pafgrnhses.com /tissue_culture.htm (107 words)

	Profile - Biocraft-Plant Tissue Culture Company
		Bio craft is a leading plant tissue culture company promoted by individuals with over 60 years of experience in agriculture.
		It is an ultra modern tissue culture laboratory with well experienced technocrats with over 15 years of experience in the field.
		The company is in a position to produce 2 —3 million plants with its present infrastructure.
	www.biocraftindia.com /profile.htm (125 words)

	Plant tissue Culture Literature
		Plant Cell, Tissue and Organ Culture 45:277-280, 1996.
		Plant cell tissue and organ culture 39, 171 - 183.
		Plant cell tissue and organ culture 41, 17 - 21.
	www.osmotek.com /lit.htm (739 words)

	Plant Tissue Culture
		Plant tissue culture (micropropagation) is a technique which will do just that for us.
		Basically the technique consists of taking a piece of a plant (such as a stem tip, node, meristem, embryo, or even a seed) and placing it in a sterile, (usually gel-based) nutrient medium where it multiplies.
		The formulation of the growth medium is changed depending upon whether you are trying to get the plant to produce undifferentiated callus tissue, multiply the number of plantlets, grow roots, or multiply embryos for "artificial seed".
	www.accessexcellence.org /LC/ST/st2bgplant.html (648 words)

	Tissue Culture - Plant Cloning (Site not responding. Last check: 2007-10-14)
		Tissue culture is used for genetically modified and natural plant propagation.
		If you have a prize-winning plant or a high-yeild crop variety that you want to reproduce so that it grows identically every time, tissue culture is a valuable too for cloning.
		The explant samples are plated on a sterile petri dish containing callus initiation medium, a mixture of hormones and nutrients.
	www.madison.k12.wi.us /west/science/biotech/tissueculture.htm (232 words)

	PPM: A Powerful Technology to Prevent and Eliminate Microbial Contamination in Plant Tissue Culture
		Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.
		If the utensil ends have touched active bacteria, fungi culture or otherwise suspected of being contaminated, they should be sterilized by autoclave or by use of an electric heating element.
		PATENT NO. 5,750,402 -- The formulation of PPM in tissue culture media at certain concentrations and the use of PPM in tissue culture at certain concentrations to prevent or eliminate microbial contamination is protected by US patent No. 5,750,402.
	www.palouse.net /kitchenculture/PPMwebsite/instructions.htm (1990 words)

	NRDC Technology offer-LOW COST PLANT TISSUE CULTURE
		Application of the technique of plant tissue culture has gradually unfolded new vistas of plant science and industry.
		Most of the studies concerning the mass propagation of economically important plants are mainly confined to the optimization of media ingredients or other culture parameters, using conventional media components and equipments are very costly.
		To be used as a substitute of Agar Agar in microbial and plant tissue culture medium.
	www.nrdcindia.com /pages/tisuecul.htm (336 words)

	Plant Tissue Culture for Home Gardners (Site not responding. Last check: 2007-10-14)
		Whilst in a research laboratory such as that in the Agronomy and Soil Science at UNE we use many high tech equipment to achieve plant production through tissue culture, it is important to note that many home gardeners and hobbyists could substitute the high tech equipment and instruments with ordinary house hold items.
		Sterile water is needed for rinsing plant material and sterile paper towelling to be used as a clean surface to work on.
		All plant material can be sterilised in diluted domestic bleach, for example White King (1/4 cup of White King + 3/4 cup of water + 1 drop of detergent- detergent acts as surfactant).
	www.une.edu.au /agronomy/hort/ptc_for_home_~1.htm (1411 words)

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