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Topic: Polyclonal antibody

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Monoclonal antibody

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	Custom Chicken Antibodies IgY - Polyclonal Antibody Production Gallus Immunotech
		As a tool in proteomics and diagnostics, there are many advantages to choosing a hen as the host to produce your custom polyclonal antibody.
		Besides polyclonal antibodies it also provides (not limited to) monoclonal antibodies, customized monoclonal antibody production, custom peptide synthesis, various anti-chicken immunoglobulins and custom antibody affinity purification.
		Its chicken antibody purification kit is cheaper than other kits on the market, while obtaining a higher yield per egg yolk.
	www.gallusimmunotech.com (323 words)

	Polyclonal antibody - Wikipedia, the free encyclopedia
		Polyclonal antibodies are antibodies that are derived from different B-cell lines.
		These antibodies are typically produced by immunization of a suitable mammal, such as a mouse, rabbit or goat.
		Adjuvants are generally used with soluble protein antigens to increase antibody titers and induce a prolonged response with accompanying memory.
	en.wikipedia.org /wiki/Polyclonal_antibody (1831 words)

	Antibody
		Antibody affinity generally increases with repeated exposure to antigen because B cells with higher affinity antigen receptors are selected to produce larger clones of antibody-secreting plasma cells.
		Antibody is synthesized on membrane-bound polyribosomes (rough endoplasmic reticulum, RER) in the cytoplasm of the B cell or plasma cell.
		Are all the molecules in the antiserum antibodies?
	microvet.arizona.edu /courses/mic419/Tutorials/antibody.html (2807 words)

	The Antibody Resource Page \| antibody
		Polyclonal antibodies are inexpensive to produce relative to the cost of monoclonal antibody technology.
		He found that antibody recognition could be probed by quantifying the amount of immunoprecipition observed upon the addition of anti-hapten polyclonal serum to a solution of a small molecule-protein conjugate.
		Using the enhancement of fluorescence as a measure of binding, Parker was able to determine the average affinity of the anti-DANS polyclonal antibodies.
	www.antibodyresource.com /antibody.html (4912 words)

	Re: what is the difference between a monoclonal and polyclonal antibody?
		If the hybridoma makes antibody, the antibody-producing genes are from the spleen B cell, not the cancer B cell, but the cells will grow indefinitely in culture due to the transformed nature of the cancer B cell.
		Polyclonals are usually generated by immunizing an animal and then removing the "immune serum" and purifying the immunoglobulin-containing fraction.
		If you have a high-specificity monoclonal, it could be more specific than a polyclonal (and often cleaner in western blot due to the lack of "contaminating" antibodies in polyclonal sera that are not directed against your protein of interest).
	www.madsci.org /posts/archives/2004-08/1092972216.Im.r.html (408 words)

	Anti SMP30 [Regucalcin,Gluconolactonase(GNL)] polyclonal antibody : Cosmo Bio Co., Ltd.
		Citrullinated proteins are known to be involved in the onset of various diseases such as articular rheumatism, Alzheimer's disease, multiple sclerosis and psoriasis.
		GFAP is an astrocyte specific marker and is known to increase its expression level in various neurodegenerative disorder such as Alzheimer's disease, multiple sclerosis and prion disease.
		Mouse glial cells in mixed cerebral cortex cultures stained with GFAP antibody (ROI003) at 1:300 dilution.
	www.cosmobio.co.jp /export_e/products/antibodies/products_sml_20061213.asp (515 words)

	Technical Resources
		Monoclonal antibodies are produced from clones of a single hybridoma cell and as a result, recognize only a single epitope on the antigen and have a defined specificity.
		Labelling you primary antibody for use in direct detection can reduce the number of steps and washes in your procedure and is less prone to background that indirect methods.
		Biotinylated or enzyme-linked antibodies are often useful for immunohistochemistry, immunoassays, and immunoblots.
	www.ucalgary.ca /antibody/technical.htm (1525 words)

	The Antibody Resource Page \| contract antibody
		Antibody Solutions - (monoclonal, polyclonal) - developer and manufacturer of novel antibodies offering a complete range of antibody services with a performance guarantee.
		Biogenesis - (monoclonal, polyclonal) - manufacturer and supplier of polyclonal and monoclonal antibodies for research and IVD manufacturing.
		They also offer polyclonal antibodies and their related antigens and conjugates, this in addition to their capabilities for large scale purification by a variety of methods.
	www.antibodyresource.com /contractantibody.html (1830 words)

	Polyclonal Solutions (Site not responding. Last check: )
		Mice are a good choice when antigen is limiting, small quantities of sera are adequate and as a pre-cursor to monoclonal antibody development.
		Rats are a good choice for mouse-derived antigens, when small quantities of sera are sufficient and as a pre-cursor to monoclonal antibody development.
		Chickens are a good choice for highly-conserved mammalian antigens and to obtain large quantities of IgY antibody from egg s.
	www.antibody.com /PolySol.html (219 words)

	FAQs - Polyclonal Antibodies (Site not responding. Last check: )
		The titre (or titer) of an antibody sample is a measure of the antibody concentration determined under a defined set of conditions.
		Serial dilutions of the antibody sample (test bleed or purified antibody) are allowed to react with a known amount of antigen.
		The antibody titre is defined as the lowest dilution to bind significantly to the antigen and is expressed as the reciprocal dilution giving an optical density (OD) reading at 450nm of 1.
	www.covalab.co.uk /main.php?pag=pages&id=48&sid=1&ssid=2&l=3 (956 words)

	SDI - Strategic BioSolutions - Genomic Antibodies™ (Site not responding. Last check: )
		Genomic Antibodies™ are obtained by injecting animals with DNA encoding the protein antigen of interest.
		This is ideal for customers seeking antibody content for their antibody arrays and protein arrays.
		Antibodies can be directed to a region of the protein antigen, such as to the ligand binding site of a receptor.
	www.strategicbiosolutions.com /GAinfo.aspx (301 words)

	Polyclonal Antibody to Neuroketals
		This antibody to NK (Catalog Number AB5611) has been shown to be immunoreactive with a neuroketal/neuroprostane-modified protein by ELISA.
		Because neuroketals and neuroprostanes are not species-specific, this antibody should be applicable detect these compounds in a number of species.
		This antibody should be a valuable tool for scientists working to understand the role of neuroketal/neuroprostane modification in neurodegenerative diseases associated with oxidative stress.
	www.chemicon.com /featured/Neuroketals.asp (372 words)

	human Survivin (C-terminus) antibody: purified antibody
		The polyclonal antibody reacts with human Survinin; the antibody was raised against residues 129-142 (C-terminus) of human Survivin, a 17 kDa protein expressed predominantly in embryonic tissues, and in most transformed human cell lines and cancers tested to date.
		Survivin is a member of the IAPs (Inhibitor of Apoptosis Proteins), a family of conserved proteins that bind and inhibit proteases involved in the apoptotic pathway.
		This polyclonal antibody can be used for immunoblotting (1:500-1:1000 dilution) of human Survivin from MOLT4 cell lysate as a positive control.
	www.ebioscience.com /ebioscience/specs/antibody_14/14-6234.htm (244 words)

	Polyclonal Antibody Development, Antiserum- Rabbit, Goat, Sheep, Chicken (Site not responding. Last check: )
		ELISA determination results of antibody titer are faxed or e-mailed to you for your data package.
		To begin a polyclonal development project, please fill out the please fill out the Polyclonal Antibody Production Checklist and e-mail or fax to MBS, and contact a service associate or call 1-800-925-9476 or 207-797-5454 to discuss the specifics of your project.
		SRV503 Purification of Polyclonal Antiserum (Rabbits, Goats, and Sheep)
	www.mainebiotechnology.com /polyclonal-antibody-development.asp (500 words)

	Aviva Antibody (Site not responding. Last check: )
		AVIVA now offers antibody services (polyclonal antibodies, monoclonal antibodies from Hybridoma or recombinant phage antibodies) to the research community.
		In most cases high titer polyclonal chicken antibodies can be obtained in one month immunization protocol.
		Since chicken antibodies do not cross-react with mammalian IgGs, bind to Fc receptors, interact with rheumatoid factors or with HAMA (human anti-murine antibodies) clear background usually is achieved using chicken IgY in immunoassays.
	www.aviva-antibody.com /services.html (605 words)

	human Stat2 p113 antibody: purified antibody
		The polyclonal antibody reacts with human Stat2; the antibody was raised against a peptide mapping to the carboxy terminus of human Stat2 p113.
		Signal Transducers and Activators of Transcription (STATs) are a family of cytoplasmic proteins that participate in cellular responses to cytokines and growth factors (1).
		It is recommended that this antibody be titrated fro optimal performance in the assay of interest.
	www.ebioscience.com /ebioscience/specs/antibody_14/14-6726.htm (436 words)

	Antibody Production, contract services
		Polyclonal Antibody Production, Custom Immunization Services: Immunization and antisera production in two rabbits or mice, or rats, or chicken, without peptide synthesis, and conjugation
		Polyclonal antibody production: Antibody purification by peptide affinity column.
		Polyclonal antibody production: Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column
	www.cytomol.com /antibody.html (224 words)

	Custom Antibody Development from Abgent
		Abgent's custom polyclonal and monoclonal antibody development team provides multiple options to quickly meet your unique needs.
		The secreted antibody will be purified by Protein G affinity chromatography and send to the customer.
		Antibodies are eluted using both pH11 and pH2.5 buffers and neutralized immediately followed by dialysis in PBS.
	www.abgent.com /doc/antibody_develop (787 words)

	Polyclonal Antibody Production
		These protocols are designed to provide a basic approach that can be extended and enhanced by using the available options.
		It can be lengthened until the desired quantity of antibody is obtained.
		The standard peptide protocol lasts 73 days, and it too can be modified until the desired quantity of antibody is obtained.
	www.bioresearchonline.com /Content/ProductShowcase/product.asp?DocID={39D69DA1-DD40-11D4-A76E-00D0B7694F32}&VNETCOOKIE=NO (429 words)

	Custom Peptide Polyclonal Antiserum! Custom antibodies for research; in rabbit, goat or chicken, using a synthetic ...
		Custom antibodies for research; in rabbit, goat or chicken, using a synthetic peptide or your recombinant protein as immunogen.
		Your custom anti-peptide polyclonal antibody project requires a collection of very different services and it is desirable to have them all from a single source.
		Place an order for your custom antibody either by filling out our order form or communicate your requirements using any of the other ways of contacting us which may be more suitable.
	www.innovagen.se /custom-peptide-polyclonal-antisera/custom-peptide-polyclonal-antisera.asp (286 words)

	New England Peptide Polyclonal Antibody Production (Site not responding. Last check: )
		After bleeds are done, the antiserum is tested for reactivity of the antibodies with peptide using an Enzyme Linked Immuno-Sorbent Assay.
		The peptide is coated onto a 96-well plate, followed by dilutions of antiserum which contain the antibody.
		If we design your antibody sequence, we guarantee a minimum ELISA titer of 1:15,000 for at least one rabbit and will keep boosting at no charge until this value is reached.
	www.newenglandpeptide.com /web/html/antitips.html (495 words)

	News Story
		The grant supports research aimed at generating human polyclonal antibodies in chicken eggs for use as passive immunotherapy to combat the growth and infection of pathogens or to block the action of toxins.
		Robert Kay, Ph.D., president and chief executive officer of Origen Therapeutics commented, "Polyclonal antibodies are very effective in fighting infectious diseases organisms, including those with potential for use as agents of bioterrorism.
		At present, human polyclonal antibodies can only be obtained from immunized human donors, limiting the number of immunogens that can safely and ethically be used to produce these therapeutics.
	www.biospace.com /news_story.aspx?StoryID=15188620&full=1 (599 words)

	Monoclonal polyclonal antibody production recombinant protein expression cloning genetic immunization
		Because You Can Trust Us For each service we offer, a technical adviser will be by your side to inform you about the latest results and about the different options available at each step of the process.
		Neurosciences antibodies alzheimer parkison beta Amyloid Prion Parkin Paired Helical Filaments alpha synuclein HLA-G adiponectin guinea pig polyclonal antibody development chinese hamster polyclonal antibody development rat monoclonal antibody development caspase secondary antibodies.
		Antibody manufacturing antibody synthesis and conjugation Bax Bcl-2 Fas PARP Retinoblastoma PCNA Cyclin D1 beta Gal VEGF EGFR JAK1 HspBP1 SIRT1 c-Fos STAT1 STAT3 STAT4 antigen retriever HLA-DR Albumin alpha-Fetoprotein Phospho-STAT1 (Ser727) Phosphotyrosine Neurofilament Vimentine.
	antibody.proteogenix.fr /services/services.html (179 words)

	Polyclonal Antibody to mouse Dicer
		Antibody in 200 ul PBS with 0.2% gelatin and 0.05% sodium azide.
		Rabbit anti-mouse Dicer polyclonal antibody was raised against a peptide corresponding to amino acids 132-150 and 1017-1033 of mouse Dicer.
		These sequences are over 90% homologous in human and rat, but testing shows no response in human and rat ovary at the recommended testing conditions.
	www.imgenex.com /antibody_details.php?catalog=IMG-538 (274 words)

	Hematech Receives Milestone Payment from its Polyclonal Antibody Collaboration with Kirin
		Currently, there is a critical shortage of immunoglobulin, or broad-spectrum polyclonal antibodies, traditionally used for the treatment of many immune system disorders.
		The Kirin technology enables the Hematech researchers to insert human antibody genes into the Hematech-derived bovine cell lines, in which their endogenous genes have been knocked out to block the production of bovine antibody.
		The Company's long term focus is on addressing the severe shortage of broad specificity human polyclonal antibodies used in the treatment of immune deficiencies.
	www.prnewswire.com /cgi-bin/stories.pl?ACCT=104&STORY=/www/story/03-05-2001/0001440403&EDATE= (550 words)

	Clontechniques OCT 2003 - BD Living Colors HcRed Polyclonal Antibody (Site not responding. Last check: )
		The new BD Living Colors™ HcRed Polyclonal Antibody is recommended for Western blot detection of HcRed1 protein, a far-red fluorescent protein from the reef coral Heteractis crispa (1, 2).
		This rabbit antibody was raised against a highly-antigenic HcRed peptide and then affinity purified using the same antigen.
		Cellular lysates were prepared 48 hr post-transfection and analyzed by Western blot with the HcRed Polyclonal Antibody (1:1,000).
	www.clontech.com /clontech/archive/OCT03UPD/LivingColor.shtml (270 words)

	MCFD2 monoclonal antibody (M01), clone 3A5-G4-Abnova Corporation
		Mouse monoclonal antibody raised against a full length recombinant MCFD2.
		Antibody Reactive Against Recombinant Protein on ELISA and Western Blot
		Immunoperoxidase of monoclonal antibody to MCFD2 (H00090411-M01) on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma.
	www.abnova.com.tw /product_search/PS_detail_antibody_gen.asp?product_select_head=antibody&catalog_id=H00090411-M01&geneid=90411&family_id= (66 words)

	NFH (Neurofilament, 200 kDa) chicken polyclonal antibody-Aves Labs
		Home: Products : Antibodies and Other Reagents : Neural Cell Type-Specific : NFH (Neurofilament, 200 kDa) chicken polyclonal antibody
		Regular vials contain 1000 ul of this antibody preparation; Sampler vials contain 200 ul of this mixture.
		Antibodies were analyzed by immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
	www.aveslab.com /ccp1328-nfh-28neurofilament--200-kda29-chicken-polyclonal-a-nfh.htm (255 words)

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