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Topic: Polymerase


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In the News (Sun 20 Dec 09)

  
  Polymerase Summary
Such studies also showed that the polymerase I is primarily involved in the repair of DNA damage, although it is also required for replication, and that polymerase III is the major replicative enzyme.
A polymerase (EC 2.7.7.6/7/19/48/49) is an enzyme whose central function is associated with polymers of nucleic acids such as RNA and DNA.
One particular polymerase, from the thermophilic bacterium, Thermus aquaticus (Taq, pronounced "tack") (PDB 1BGX, EC 2.7.7.7) is of vital commercial importance due to its use in the polymerase chain reaction, a widely-used technique of molecular biology.
www.bookrags.com /Polymerase   (706 words)

  
 BioMed Central | Full text | The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient ...
Three structurally and functionally distinct DNA polymerases, known as DNA polymerases α, δ, and ε (Pol α,-δ, and -ε, respectively), are required for chromosomal DNA replication in yeasts [1-3].
Because this portion of the enzyme includes all known DNA polymerase and exonuclease motifs, these results suggest that the DNA polymerase activity of Pol ε is dispensable for chromosomal DNA replication in yeast [15,16,27].
However, because the amino-terminal portion of Pol ε, that is required for its DNA polymerase- and exonuclease activities, is dispensable for yeast DNA replication, repair, and viability [15,16,27], the role of Pol ε during DNA replication has remained obscure.
www.biomedcentral.com /1471-2091/7/21   (5878 words)

  
 genome.gov | PCR Fact Sheet
Because significant amounts of a sample of DNA are necessary for molecular and genetic analyses, studies of isolated pieces of DNA are nearly impossible without PCR amplification.
To amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or separates into two pieces of single-stranded DNA.
Next, an enzyme called "Taq polymerase" synthesizes - builds - two new strands of DNA, using the original strands as templates.
www.genome.gov /10000207   (313 words)

  
 3D Structures from Darst Lab
Transcription elongation factors stimulate the activity of the RNA polymerase by increasing the overall elongation rate and the completion of RNA chains.
The three-dimensional structure RNA polymerase holoenzyme was determined by electron microscopy of negatively stained, two-dimensional crystals tilted at various angles to the incident electron beam in Roger Kornberg lab (Stanford University) by Darst et al.
The structure of Yeast RNA Polymerase II (pol II) was determined by 3D reconstitution from electron micrographs of 2D crystals at ~16 Å in Roger Kornberg lab (Stanford University) by Darst et al.
www.rockefeller.edu /labheads/darst/structures.htm   (602 words)

  
 MSN Encarta - Romania
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 Bacterial RNA Polymerase   (Site not responding. Last check: 2007-10-26)
Structure of RNA polymerase holoenzyme - from Seth Darst's laboratory at Rockefeller University
It is clear that the sigma subunit is intimately associated with the enzyme and literally "buries" into the interior of the complex.
It directs RNA polymerase to the promoter and ensures that transcription is initiated only where it is supposed to be initiated.
www.mun.ca /biochem/courses/3107/Lectures/Topics/RNAP_bacterial.html   (1346 words)

  
 Subunit Structure of Mitochondrial DNA Polymerase from Drosophila Embryos -- Olson et al. 270 (48): 28932 -- Journal of ...
Subunit Structure of Mitochondrial DNA Polymerase from Drosophila Embryos -- Olson et al.
That and the potent inhibition by the rabbit antiserum of the DNA polymerase and 3`
Catalytic Subunit of Mitochondrial DNA Polymerase from Drosophila Embryos.
www.jbc.org /cgi/content/abstract/270/48/28932   (635 words)

  
 PCR (Polymerase Chain Reaction) - HIV: health and medical information about HIV and AIDS
PCR (Polymerase Chain Reaction) is a key technique in molecular genetics that permits the analysis of any short sequence of DNA (or RNA) without having to clone it.
Annealing: At 54°C, hydrogen bonds form and break between the single-stranded "primer" and the single-stranded "template." (The template provides the pattern to be copied.) The more stable bonds last longer and on that little length of double-stranded DNA (the joined primer and template), the polymerase attaches and starts copying the template.
As a result, the attraction, created by the hydrogen bonds, of the primers to the template is stronger than the forces breaking these attractions.
www.medicinenet.com /pcr_polymerase_chain_reaction/article.htm   (525 words)

  
 Invitrogen - PlatinumTaq DNA Polymerase
Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with proprietary antibody that inhibits polymerase activity.
This results in a DNA polymerase which is activated in a temperature dependent manner (at 94ºC.) during the start of PCR.
II Preamplification System and cDNA was amplified using gene specific primers for a 1.5 kb region of the DNA polymerase E (pol E) mRNA.
www.invitrogen.com /content.cfm?pageid=554   (510 words)

  
 Catalytic Subunit of Mitochondrial DNA Polymerase from Drosophila Embryos. CLONING, BACTERIAL OVEREXPRESSION, AND ...
A full-length cDNA of the catalytic subunit of mitochondrial DNA polymerase from Drosophila embryos has been obtained, and its nucleotide sequence was determined.
The cDNA clone encodes a polypeptide with a deduced amino acid sequence of 1145 residues and a predicted molecular mass of 129.9 kDa.
exonuclease activity in the polymerase catalytic subunit of animal mitochondrial DNA polymerase.
www.jbc.org /cgi/content/abstract/271/38/23389   (784 words)

  
 The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA ...
The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I -- Bedford et al.
DNA whereas T7 DNA polymerase is responsible for the replication
DNA Polymerases that Propagate the Eukaryotic DNA Replication Fork
www.pnas.org /cgi/content/short/94/2/479   (730 words)

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