
	Where results make sense

Topic: Ponceau s

In the News (Fri 17 Feb 12)

EXECUTIVE POWER

Iran

ANALYSIS

Pakistan

Family compact

	2.0 Protein Isolation
		Examples of the S values and corresponding molecular weights are the molecular weight of serum albumin of 69,000 with an S of 4.6, and a molecular weight of a monoclonal immunoglobulin G was determined to be 178,000 and its S was determined as 7.08.
		Ponceau S The sensitivities of detection with two of these dyes are: Coomassie Blue detects as little as 0.1 micrograms protein (about 2 picomoles) and the silver stain detects even less, 0.02 micrograms protein.
		In the clinical chemistry laboratory Ponceau S is used to stain blood serum proteins separated on agarose or cellulose acetate media.
	www.uth.tmc.edu /uth_orgs/educ_dev/fellowship/ross/PROTEINSLESSON2.html (5862 words)

	New Page 2
		S 24/25 Avoid contact with skin and eyes.
		S 28A After contact with skin, wash immediately with
		S 45 In case of accident or if you feel unwell, seek
	www.sefsc.noaa.gov /HTMLdocs/ponceau_s.htm (658 words)

	Ponceau S - Wikipedia, the free encyclopedia
		Ponceau S, Acid Red 112, or C.I. is a sodium salt of a diazo dye that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or PVDF membranes (Western blotting), as well as on cellulose acetate membranes.
		A Ponceau S stain is easily reversed with water washes, facilitating subsequent immunological detection.
		Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% TCA and 30% sulfosalicylic acid.
	en.wikipedia.org /wiki/Ponceau_S (132 words)

	Western blot - Pounceau S staining problem - BioForum
		I've had problems with ponceau stainings of PVDF and instead of the standard Sigma (0.1% ponceau, 5% acetic acid), i made my own ponceau as 0.2% ponceau in 3% TCA, and that worked.
		Unfortunately, the Ponceau lost and got tipped down the sink as I thought it had gone off (it does go off and there was no open or receipt date).
		Ponceau stain requires low pH to work, hence the glacial acetic acid or TCA used to prepare it.
	www.protocol-online.org /forums/index.php?showtopic=4811 (1227 words)

	Interaction of Focal Adhesion Kinase with Cytoskeletal Protein Talin -- Chen et al. 270 (28): 16995 -- Journal of ...
		The membranes were then stained with Ponceau S to show that similar amounts of fusion proteins were present in the binding assays.
		Ponceau S staining of the same membranes showed that similar amounts of the fusion proteins were present in all samples (Fig.
		B, the membranes were then stained with Ponceau S to verify that similar amounts of fusion proteins were present in all samples in the binding experiments (lanes2-4).
	www.jbc.org /cgi/content/full/270/28/16995 (5297 words)

	Pierce Biotechnology - MemCode Reversible Protein Stain Kits
		Those who use Ponceau S for staining are very familiar with its major shortcomings and will welcome Pierce MemCode Reversible Protein Stain, which overcomes the disadvantages of Ponceau S-based homemade and commercial formulations.
		Blot B was stained with 0.1% Ponceau S in 5% acetic acid for 5 minutes.
		The Panel B membrane was stained with 0.1% Ponceau S stained for 5 minutes and destained according to the published protocol.
	www.piercenet.com /Objects/View.cfm?type=ProductFamily&ID=6B411726-6D96-44E1-88B6-6B7901BC2DBF (789 words)

	Western Blots
		Ponceau is reversable protein dye; this staining allows you to check the transfer and see any bubbles that might have been in the sandwich, and to check the loading of the gel.
		The ponceau solution is reusable, so pour it back in the bottle after use.
		To remove the ponceau and block the blot, soak in blotto for >30 sec.
	info.med.yale.edu /mbb/koelle/protocols/protocol_western_blot.html (1242 words)

	Fenteany Research Group: Methods (Site not responding. Last check: 2007-10-11)
		Ponceau S: 0.1% w/v in 1% Acetic acid, reusable
		(Optional) Stain blot for proteins using ponceau S. Wash blot briefly in water then incubate in ponceau S solution for 1 min.
		Rinse blot several times with TBS-Tween to remove ponceau S. Block blot in 5% milk/TBS/Tween in the container, 2hr, RT, on shaker; OR o/n in 4oC on a rocker.
	www.biochemweb.org /fenteany/methods/western_blot.html (636 words)

	individual book page (Site not responding. Last check: 2007-10-11)
		In this volume, the focus is on the authors who competed for the prize of 1835, the only year in which the theme of the contest was restricted to Amerindian (particularly Delaware) linguistics.
		The two competitors, Peter S. Du Ponceau and Constantine S. Rafinesque, both lived in the United States, but were of French and Swiss extraction and able to write their essays in French.
		Then follows a reprint with annotations of Du Ponceau's famous “Mémoire sur le système grammatical des langues de quelques nations indiennes de l'Amérique du Nord,” published in 1838 and based upon his prizewinning 1835 Volney manuscript which is no longer extant.
	wings.buffalo.edu /linguistics/ssila/books/indbook/b551.htm (248 words)

	Technical Support - Applications (Site not responding. Last check: 2007-10-11)
		We performed an internal study on the effectiveness of 1PS media using an aqueous soluble dye, Ponceau S and two halogenated solvents Dichloromethane and Chloroform.
		The Ponceau S dye was made up into a 0.02% solution in both 1M HCl and 1M NaOH.
		A standard curve was constructed and the OD max was taken in both of the Dye solutions and against the blank readings in all cases.
	www.whatman.com /repository/documents/s3/tech_appli_010.html (416 words)

	Redistribution of pulmonary EC-SOD after exposure to asbestos -- Tan et al. 97 (5): 2006 -- Journal of Applied ...
		Ponceau S staining was performed by immersing blots in a 1%
		Mossman BT, Marsh JP, Sesko A, Hill S, Shatos MA, Doherty J, Petruska J, Adler KB, Hemenway D, Mickey R, Vacek P, and Kagan E. Inhibition of lung injury, inflammation, and interstitial pulmonary fibrosis by polyethylene glycol-conjugated catalase in a rapid inhalation model of asbestosis.
		Uchida K, Kato Y, and Kawakishi S. A novel mechanism for oxidative cleavage of prolyl peptides induced by the hydroxyl radical.
	jap.physiology.org /cgi/content/full/97/5/2006 (3813 words)

	Du Ponceau on Chinese
		Letter from Peter S. Du Ponceau to Captain Basil Hall, R. Philadelphia, 7th July, 1828.
		It is therefore with great pleasure that I comply with your request, in giving some further development to the ideas which I then threw out to you, and which derive all their value from your having thought them worthy to be kept in mind.
		PETER S. Capt. BASIL HALL, R. S., andc.
	www.pinyin.info /readings/texts/duponceau.html (3146 words)

	Mitogen-activated protein kinase (MAPK) pathway activation: effects of age and acute exercise on human skeletal muscle ...
		The exercise involved a concentric and an eccentric component, in which the subject was required to lift the weight to full knee extension (concentric) and lower the weight to the starting position (eccentric).
		Blots were also stained with Ponceau S (Sigma) to verify equal loading and transfer.
		The purpose of this investigation was to examine the interaction of age and the activation of MAPK signalling proteins in response to resistance exercise.
	jp.physoc.org /cgi/content/full/547/3/977 (6649 words)

	Support, RNA Electrophoresis: Protocol for Semi-dry Transfer for Western Blotting
		Alternatively, the extent of protein transfer can be determined by staining the polyacrylamide gel after the transfer or by staining the protein directly on the membrane.
		Proteins on PVDF membranes can be visualized with the PageBlue™ Protein Staining Solution, while nitrocellulose and PVDF membranes can be stained with India Ink, Amido Black or Ponceau S and other dyes.
		Stain the membrane with the Ponceau S staining solution under agitation.
	www.fermentas.com /techinfo/electrophoresis/pproteintransfer.htm (410 words)

	[No title]
		The initial current should be approximately 100 mA/gel seq level0 \ROMANIII.seq level1 \h \r0 Staining with Ponceau* S seq level1 \*ALPHABETICA.
		Place the membranes in a staining container and add enough Ponceau S stain to float the membranes.
		Ponceau S Stain 6 ml Ponceau S concentrate (Sigma: 2% Ponceau S; 30% TCA; 30% sulfosalicylic acid) to 60 ml with water.
	www.trnty.edu /faculty/boomsma/methods/protein/WesternBlot.doc (1163 words)

	Western Blotting (Site not responding. Last check: 2007-10-11)
		Ensure good protein transfer by staining for 1 minute with Ponceau S followed by complete destain in distilled water.
		Place membrane into blocking solution for 30 minutes to 2 hours at room temperature, or overnight at 4°C. Wash the membrane briefly in wash buffer and then place in primary antibody diluted in wash buffer (a concentration of 1-10µg/ml is generally acceptable).
		0.1g Ponceau S (Sigma P3504), distilled water 95ml, acetic acid 5mls.
	www.serotec.com /html/ws11.htm (352 words)

	viral immunity and pathogenesis group. western blot with platelets.
		:: w e s t e r n b l o t w i t h p l a t e l e t p r o t e i n s ::
		Western blot is a wide used technique to identify a target protein/s for the certain antibody.
		Ponceau S, 1 L, keep at RT = Ponceau S, 1g + acetic acid (glacial), 50 ml + ddH
	www.icp.ucl.ac.be /mexp/vipg_plwb.html (683 words)

	Loyola Univ. Health Univ. Health Sys- Microbiology & Immunology: Knight Lab: Western Blot
		Ponceau S staining can be done to reveal a protein profile on membrane.
		Stain membrane 10 min with 1X Ponceau S (diluted in water), discard Ponceau S solution, rinse membrane several times with large volume PBS or water (100-150 ml) to remove red background.
		Visualize by copying on Xerox machine and remove Ponceau S staining completely by washing in PBS or in water.(see Molecular Cloning, 1989, p.18.67 by Maniatis at al.)
	www.meddean.luc.edu /lumen/DeptWebs/Microbio/KNIGHT/protoc/western.htm (533 words)

	A Protein (M9) Associated with Monoclonal Antibody-Mediated Agglutination of Mycoplasma gallisepticum Is a Member of ...
		(C) The immunoaffinity- and gel-purified protein sample was concentrated, subjected to SDS-PAGE, blotted onto a PVDF membrane, and stained with Ponceau S. One of four identical lanes is shown, each of which contained 10 µg of M9 protein.
		M9 protein bands by staining the membrane with Ponceau S (1),
		Liu, L., Panangala, V. S., Dybvig, K. Trinucleotide GAA Repeats Dictate pMGA Gene Expression in Mycoplasma gallisepticum by Affecting Spacing between Flanking Regions.
	iai.asm.org /cgi/content/full/66/11/5570 (3577 words)

	Boston Bioproducts Inc - Protein Molecular Weight Markers
		Proteins immobilized on the membrane can be stained with Ponceau S or India ink.
		Staining with Ponceau S is compatible with all methods of immunological detection because the stain is washed during the detection process.
		Add fresh Ponceau S soln and incubate for 5-15 min at RT with agitation.
	www.bostonbioproducts.com /products/RF_Protein_Analysis/3.htm (1184 words)

	Thyroid Hormone Regulates the Cell Cycle Inhibitor p27Kip1 in Postnatal Murine Sertoli Cells -- Holsberger et al. 144 ...
		Membranes were stained with Ponceau S to verify even transfer and equal loading of protein, then p27
		expression was normalized to a family of 60-kDa proteins detected by Ponceau S staining and quantitated.
		Jirawatnotai S, Moons DS, Stocco CO, Franks R, Hales DB, Gibori G, Kiyokawa H 2003 The cyclin-dependent kinase inhibitors p27Kip1 and p21Cip1 cooperate to restrict proliferative life span in differentiating ovarian cells.
	endo.endojournals.org /cgi/content/full/144/9/3732 (3940 words)

	Nitrocellulose (Site not responding. Last check: 2007-10-11)
		The size and purity of the isolated proteins can be assessed by using a reversible stain such as Ponceau S. Generally, a band that can be visualized by Ponceau S contains enough protein for use in a custom antibody project.
		Note: It is important to use pure Nitrocellulose as Nitrocellulose reinforced with acetate or plastics can interfere with processing steps used by Quality Bioresources, Inc.
		Stain nitrocellulose with 0.1% Ponceau S (w/v) in 5% acetic acid (ex.
	www.qualbio.com /nitrocel.htm (168 words)

	The Documentary History of the Supreme Court of the United States, 1789-1800; Volume 7; Edited by Maeva Marcus
		Peter S. Du Ponceau to Edward Livingston-August 5, 1796
		Peter S. Du Ponceau to Edward Livingston-August 8, 1796
		Peter S. Du Ponceau to Edward Livingston-August 10, 1796
	www.columbia.edu /cu/cup/catalog/data/023112/0231126468.HTM (1556 words)

	Wnt-3A Enhances Bone Morphogenetic Protein-2-mediated Chondrogenesis of Murine C3H10T1/2 Mesenchymal Cells -- Fischer ...
		Equal protein loading was confirmed by either Coomassie Blue staining of parallel gels or Ponceau S staining of the immunoblot.
		Equal protein loading was confirmed by Ponceau S staining of the immunoblot.
		B, Ponceau S staining of the above immunoblot to visualize equivalent protein loading.
	www.jbc.org /cgi/content/full/277/34/30870 (5069 words)

	PONCEAU 4R - Food colors - WELCOME TO SAUJANYA DYE CHEM- MANUFACTURERS & EXPORTER OF DYES,
		QUINOLINE YELLOW-WS Ponceau 4R consists essentially of trisodium 2-hydroxy-1(4-sulfonato-1-naphthylazo) naphthalene-6,8-disulfonate and subsidary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components.
		Ponceau 4R is described as the sodium salt.
		The calcium and the potassium salt are also permitted.
	www.saujanya.com /ponceau.htm (157 words)

	Dietary Soy Protein Isolate and Isoflavones Modulate Hepatic Thyroid Hormone Receptors in Rats -- Xiao et al. 134 (4): ...
		Potter, S. (1998) Soy protein and cardiovascular disease: the impact of bioactive components in soy.
		Wright, S. & Salter, A. (1993) The effect of dietary casein and soy protein on cholesterol metabolism in hamsters.
		Xiao, C. W., Yan, X., Li, Y., Reddy, S. & Tsang, B. (2003) Resistance of human ovarian cancer cells to tumor necrosis factor alpha is a consequence of nuclear factor kappaB-mediated induction of Fas-associated death domain-like interleukin-1beta-converting enzyme-like inhibitory protein.
	jn.nutrition.org /cgi/content/full/134/4/743 (4621 words)

	Role of Protein Kinase C in Control of Ethanol-Modulated beta -Endorphin Release from Hypothalamic Neurons in Primary ...
		After transfer, the membrane was stained with ponceau S solution.
		Battaini F, Vesco R, Govoni S and Trabucchi M (1989) Chronic alcohol consumption decreases the phorbol ester binding in selected rat brain area.
		Kharbanda S, Nakamura T and Kufe D (1993) Induction of the c-jun proto-oncogene by a protein kinase C-dependent mechanism during exposure of human epidermal keratinocytes to ethanol.
	jpet.aspetjournals.org /cgi/content/full/301/1/119 (4641 words)

	BioTrace™ NT Nitrocellulose Transfer Membrane for Western Transfer and Sequencing
		After Western transfer, membranes should be rinsed in transfer buffer to remove excess gel fragments.
		Ponceau S Staining Protocol for Reversible Total Protein Detection
		Soak membranes in 0.01% Ponceau S, 3% trichloroacetic acid for 1 minute.
	www.pall.com /924_33009.asp?sectionid=instructions_for_use (496 words)

	RGS2/G0S8 is a selective inhibitor of Gqalpha function -- Heximer et al. 94 (26): 14389 -- Proceedings of the ...
		to be detected by Ponceau S staining (Fig.
		S for 1 hr at 30°C. Activated Gq (1 nM final concentration)
		S. Heximer, R. Knutsen, X. Sun, K. Kaltenbronn, M.-H. Rhee, N. Peng, A. Oliveira-dos-Santos, J. Penninger, A. Muslin, T. Steinberg, J. Wyss, R. Mecham, and K. Blumer
	www.pnas.org /cgi/content/full/94/26/14389 (4586 words)

	ponceau 4r by Standardcon - Manufacturer & Exporter of Aluminium Lake Colours & Food Colours
		ponceau 4r by Standardcon - Manufacturer and Exporter of Aluminium Lake Colours and Food Colours
		A red synthetic coal tar or azo dye.
		Fields marked with * are compulsory, as it will help us to respond
	www.standardcon.com /ponceau4r.htm (64 words)

	Du Ponceau: Jurisdiction (1824)
		"A Dissertation on the nature and extent of the Jurisdiction of the Courts of the United States, being a Valedictory Address delivered to the students of the Law Academy of Philadelphia, at the close of the academical year, on the 22d April, 1824, by Peter S. Du Ponceau, LL.D. Provost of the Academy.
		To which are added, A brief sketch of the National Judiciary Powers exercised in the United States prior to the adoption of the present Federal Constitution, by Thomas Sergeant, Esq.
		By Peter S. Du Ponceau, LL.D. Constitution of the United States,...
	www.constitution.org /cmt/psdp/juris.htm (14589 words)

Try your search on: Qwika (all wikis)