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Topic: Potyvirus

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	Potyvirus
		The potyvirus group (named for its prototypical member, potato virus Y (PVY)) is the largest of the 34 plant virus groups and families currently recognised (Ward & Shukla, 1991).
		Potyvirus virions are non-enveloped (Langenberg & Zhang, 1997) filamentous particles, 680 to 900nm long and 11 to 15nm wide (Dougherty & Carrington, 1988; Riechmann, et al., 1992).
		Some potyvirus strains have a bipartite genome which contains identical proteins but are processed slightly different due to there being two strands of RNA and hence two polyproteins.
	www.uq.edu.au /vdu/VDUPotyvirus.htm (1932 words)

	APSnet Feature - Plum Pox Potyvirus Disease of Stone Fruits (Site not responding. Last check: 2007-10-29)
		Following recognition of symptomatic peach fruit and the positive confirmation of the causal agent of the disease in October, 1999, an official announcement of the presence of the dreaded disease in Adams County, Pennsylvania was made jointly by the Animal and Plant Health Inspection Service and the Pennsylvania Department of Agriculture.
		Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins.
		Verhoeven, J.Th.J., de Haas, A.M., Roenhorst, J.W. Outbreak and eradication of plum pox potyvirus in the Netherlands.
	www.apsnet.org /online/feature/PlumPox (4224 words)

	USNA/FNPRU: Dr. John Hammond
		The 3’-terminal region of several potyvirus isolates from Ornithogalum and Lachenalia, including thecoat protein gene, have been cloned and sequenced for comparison, and constructs are being prepared for transformation of interspecific Ornithogalum hybrids in order to evaluate virus resistance.
		To examine the use of potyvirus coat protein as a carrier for vaccine-significant epitopes.
		Hammond, J., and Kamo, K. Potyvirus resistance derived from antisense RNA and native or chimeric coat protein gene.
	www.usna.usda.gov /Research/~jh.html (2286 words)

	Recombination of Engineered Defective RNA Species Produces Infective Potyvirus In Planta -- Gal-On et al. 72 (6): 5268 ...
		Infectious in vitro RNA transcripts derived from the cloned cDNA of the cucurbit potyvirus, zucchini yellow mosaic virus.
		Analysis of the VPg-proteinase (NIa) encoded by the tobacco etch potyvirus: effects of mutations on subcellular transport, proteolytic processing, and genome amplification.
		Evidence that the potyvirus P1 protein functions as an accessory factor for genome amplification.
	jvi.asm.org /cgi/content/full/72/6/5268 (1942 words)

	DanforthCenter.org
		Balint, R., Plooy, I., and Steele, C. The nucleotide sequence of zucchini yellow mosaic potyvirus.
		Hammond, J., and Hammond, R. Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene.
		Xiao, X. W., Frenkel, M. J., Ward, C. W., and Shukla, D. Nucleotide sequence of the 3'-terminal region of the genome confirms that pea mosaic virus is a strain of bean yellow mosaic potyvirus.
	www.danforthcenter.org /iltab/potyviridae/references.htm (2141 words)

	ISMB 2006: Fortaleza, Brazil, August 6-10
		Potyvirus are ssRNA(+) viruses belonging to the family Potyviridae.
		HC-Pro is a multifunctional protein that contains three intrinsic functional domains: an N-terminal domain essential for aphid transmission, a central domain that binds nucleic acid in a nonspecific manner and is implicated in a variety of processes, including suppression of RNA silencing and a C-terminal domain that is reminiscent of cysteine-peptidases.
		Moreover, the economically important aphid-transmitted species of the genus Potyvirus were clustered together and showed a close phylogenetic relationship to members of the genus Rymovirus.
	ismb2006.cbi.cnptia.embrapa.br /poster_abstract.php?id=C-30 (1044 words)

	IDENTIFICATION OF PLANT VIRAL SYNERGISM GENES
		However, it is not clear that the positive correlation between nonpotyviral accumulation and symptom severity reflects a cause and effect relationship, and, in fact, the basis of synergistic disease is not understood.
		It is not clear how co-infection with a potyvirus influences PVX pathogenesis, but active replication and/or the concomitant gene expression from the potyviral genomic RNA is critical (Goodman and Ross, 1974 a and b).
		Jordan, R.L., and Hammond, J. Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies.
	www.isb.vt.edu /brarg/brasym95/shi95.htm (3678 words)

	A Cysteine-Rich Plant Protein Potentiates Potyvirus Movement through an Interaction with the Virus Genome-Linked ...
		The Potyvirus group is the major genus of the family Potyviridae.
		A potyvirus polymerase interacts with the viral coat protein and VPg in yeast cells.
		Potyvirus genome-linked protein (VPg) determines pea seed-borne mosaic virus pathotype-specific virulence in Pisum sativum.
	jvi.asm.org /cgi/content/full/78/5/2301 (6054 words)

	Canadian Food Inspection Agency - Plant Health Risk Assessment - Plum Pox Potyvirus - Fact Sheet (Site not responding. Last check: 2007-10-29)
		Infected fruits show chlorotic spots or rings, and diseased plums and apricots are deformed with internal browning of the flesh and pale rings or spots on the stones.
		Plum pox potyvirus is a filamentous virus with particles 750
		Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of
	www.inspection.gc.ca /english/sci/surv/plumpox/ppvfs_e.shtml (2244 words)

	Plum Pox Potyvirus
		Four strains of the plum pox potyvirus exist and they are usually transmitted by aphids, or the movement of nursery stock, budwood and grafting.
		Plum pox potyvirus (PPV - EPPO A2 quarantine pest) is naturally transmitted in orchards by several aphid species, and is also spread by...
		Plum pox potyvirus (PPV) is a devastating disease of wild and cultivated Prunus species, which include plums, peaches, apricots, nectarines, and many other stone fruits.
	www.cps-scp.ca /pathologynews/plumpoxpotyvirus.htm (8991 words)

	IDENTIFICATION OF PLANT VIRAL SYNERGISM GENES
		Plant viral synergisms may be divided into two major classes: the potyvirus-associated synergisms, in which one of the synergistic pair of viruses is a member of the potyvirus group of plant viruses, and the much less well characterized non-potyvirus synergisms, in which neither virus is a member of this group.
		The best studied of the potyvirus -associated synergisms is the interaction of PVX with a number of viruses in the potyvirus group, including potato virus Y (PVY), tobacco vein mottling virus (TVMV) and tobacco etch virus (TEV).
		PVX/potyviral synergism does not require a co-infecting potyvirus, and synergistic disease is mimicked in transgenic plants expressing the 5' proximal region of the potyviral genome and infected singly with PVX (Vance et al., 1995).
	www.nbiap.vt.edu /brarg/brasym96/vance96.htm (1157 words)

	[No title]
		Merits, A., Guo, D., and Saarma M. (1998) VPg, coat protein and five non-structural proteins of potato A potyvirus bind RNA in a sequence-unspecific manner.
		Andrejeva, J., Puurand, Ü, Merits, A., Rabenstein, F., Järvekülg, L., and Valkonen, J.P.T. Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus-host interactions and the same CP motif affects virus transmission and accumulation.
		Merits, A., Guo, D., Järvekülg, L., and Saarma, M. Biochemical and genetic evidence for interaction of potato A potyvirus encoded proteins P1 and P3 with proteins of putative replication complex.
	www.biocenter.helsinki.fi /bi/plantvir/publicat.html (702 words)

	[No title]
		All members of the potyvirus group (about 180) encode a protein, called the cylindrical inclusion protein (CIP), that aggregates in the cytoplasm of infected cells to form inclusions resembling pinwheels.
		This makes sense since the potyvirus particles are long and flexuous.Plant viruses shorter or longer than those of the potyvirus group do not induce cylindrical inclusions but often occur in very large numbers intracellularly.
		Calder, V.L., and Ingerfeld, M. The roles of the cylindrical inclusion protein of a potyvirus in the induction of vesicles and in cell-to-cell spread.
	www.helsinki.fi /~kankila/diagnos/ref1.html (907 words)

	Identification of an N-terminal domain of the plum pox potyvirus CI RNA helicase involved in self-interaction in a ...
		Potyvirus CI RNA helicase is a protein involved in RNA genome
		Arbatova, J., Lehto, K., Pehu, E. and Pehu, T. Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells.
		Roberts, I. M., Wang, D., Findlay, K. and Maule, A. Ultrastructural and temporal observations of the potyvirus cylindrical inclusions (CIs) show that the CI protein acts transiently in aiding virus movement.
	vir.sgmjournals.org /cgi/content/full/82/3/677 (4831 words)

	ARS \| Publication request: Detection and Characterization of Euphorbia Ringspot Potyvirus (Site not responding. Last check: 2007-10-29)
		A commercially-grown plant exhibiting ring-shaped chlorotic spots and leaf and flower deformations was shown to contain 650-850 nm flexuous particles and tested positive for potyvirus in ELISA using our genus Potyvirus broad spectrum reacting PTY-1 monoclonal antibody.
		Total RNA extracts from infected leaves were used in RT-PCR assays with generic potyvirus (and, later, EuRSV-specific) primers which amplify various highly conserved 335, c.700, or c.1600bp fragments from the 3' terminus of most potyviruses which includes the potyviral coat protein (CP) and the 3' non-coding region (3'NCR).
		Pairwise alignments and Clustal W analysis confirm the classification of this poorly described virus as a potyvirus member.
	ars.usda.gov /research/publications/publications.htm?seq_no_115=162554 (355 words)

	New Disease Reports - Basella rugose mosaic virus, a new potyvirus infecting Basella rubra
		The only potyvirus previously reported to infect a member of the Basellaceae (Ullucus tuberosus) is Ullucus mosaic virus (Brunt et al., 1982).
		According to the species demarcation criteria for the Potyviridae (Adams et al., 2005) the BR isolate is a new potyvirus and is herein designated as Basella rugose mosaic virus (BaRMV).
		Identification and molecular characterization of Zantedeschia mild mosaic virus, a new calla lily-infecting potyvirus.
	www.bspp.org.uk /ndr/july2006/2006-20.asp (376 words)

	Bioline International Official Site (site up-dated regularly) (Site not responding. Last check: 2007-10-29)
		Antisera against TeMV (Shoyinka et al., 1987) and CMV (G. Thottappilly, unpublished) were prepared while the commercial universal potyvirus (PTY) monoclonal antibodies were purchased from AGDIA Inc., U.S.A. The antiserum to PVMV was the one used in a previous investigation (Lana et al., 1975).
		In the case of universal potyvirus monoclonal antibodies, plate trapped (antigen coated) indirect ELISA was used.
		After washing, universal potyvirus antibodies at a dilution of 1/1000 were added.
	www.bioline.org.br /request?cs98008 (2499 words)

	Welcome To Paradise- River Hill High School
		The symptomatic Tricyrtis was analyzed by ELISA using a panel of potyvirus specific monoclonal antibodies (McAbs).
		One-on-one comparisons of the CP amino acid and 3’ NCR nucleotide sequences in the Tricyrtis virus revealed less than 90.0% identity with other members of the potyvirus group, which suggests that this virus is a unique member of the potyvirus group.
		This is believed to be the first report of a potyvirus, not to mention any virus infection, in the genus Tricyrtis.
	www.howard.k12.md.us /riverhill/mstresearchtylervanburen.html (321 words)

	The Eukaryotic Translation Initiation Factor 4E Controls Lettuce Susceptibility to the Potyvirus Lettuce mosaic virus ...
		Keller KE, Johansen IE, Martin RR, Hampton RO (1998) Potyvirus genome-linked protein (VPg) determines Pea seed-borne mosaic virus pathotype-specific virulence in Pisum sativum.
		Montesclaros L, Nicol N, Ubalijoro E, Leclerc-Potvin C, Ganivet L, Laliberté JF, Fortin MG (1997) Response to potyvirus infection and genetic mapping of resistance loci to potyvirus infection in Lactuca.
		Murphy JF, Rhoads RE, Hunt AG, Shaw JG (1990) The VPg of tobacco etch virus RNA is the 49-kDa proteinase or the N-terminal 24-kDa part of the proteinase.
	www.plantphysiol.org /cgi/content/full/132/3/1272 (6581 words)

	FURTHER CHARACTERIZATION OF A POTYVIRUS IN ALSTROEMERIA ENDOWMENT SERIES
		With indirect ELISA, the virus did not react to alstroemeria mosaic virus antiserum and a broad spectrum potyvirus monoclonal antibody.
		However, the dissociated viral coat protein reacted strongly to a potyvirus group specific polyclonal antibody in western immunoblot.
		Since attempts to transmit this virus mechanically from Alstroemeria to other common virus indexing plants failed, we were unable to obtain single lesion isolates.
	www.actahort.org /books/377/377_5.htm (181 words)

	Expression of functionally active helper component protein of Tobacco etch potyvirus in the yeast Pichia pastoris -- ...
		Lopez-Moya, J. J., Wang, R. and Pirone, T. Context of the coat protein DAG motif affects potyvirus transmissibility by aphids.
		Peng, Y. H., Kadoury, D., Gal-On, A., Huet, H., Wang, Y. and Raccah, B. Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions.
		Sasaya, T., Torrance, L., Cowan, G. and Ziegler, A. Aphid transmission studies using helper component proteins of potato virus Y expressed from a vector derived from potato virus X. J Gen Virol 81, 1115–1119.
	vir.sgmjournals.org /cgi/content/full/85/1/241 (4706 words)

	USNA/FNPRU: Dr. Ramon Jordan
		Gillaspie, Jr., A.G., Hopkins, M.S., Pinnow, D.L., and Jordan, R.L. Characterization of a potyvirus of the bean yellow mosaic virus subgroup in Sesbania speciosa germplasm.
		Detection and characterization of a new potyvirus, Impatiens flower break virus, infecting New Guinea Impatiens.
		Jordan, R.L. and Hammond, J. Expression of viral genes and viral and antiviral proteins in transgenic plants to confer virus resistance.
	www.usna.usda.gov /Research/~rlj.html (953 words)

	Potyvirus WIT (Site not responding. Last check: 2007-10-29)
		Potyviruses belong to the family Potyviridae: named after their prototypical member, potato virus Y. Potyviruses comprise the largest group of viruses that infect plants and include up to 30% of all plant viruses.
		Potyviruses cause significant agricultural, horticultural and ornamental crop losses, and include the most destructive and most feared viral disease of stonefruit (e.g, peaches, plums, apricots), the Plum pox potyvirus (PPV).
		The viruses are transmitted by a number of means including a vector (insect Aphididae), grafting, contact between plants, seed, pollen to the seed, pollen to the pollinated plant.
	www.ag.iastate.edu /departments/micro/grad/gdpotyvirus.html (116 words)

	poty.html
		Maiti, I. B., Murphy, J. F., Shaw, J. G., and Hunt, A. (1993) Plants that express a potyvirus proteinase gene are resistant to virus infection.
		Graybosch, R., Hellmann, G. M., Shaw, J. G., Rhoads, R. E., and Hunt, A. Expression of a potyvirus non-structural protein in transgenic tobacco.
		Brantley, J. and Hunt, A. The N-terminal protein of the polyprotein encoded by the potyvirus tobacco vein mottling virus is an RNA binding protein.
	www.uky.edu /~aghunt00/poty.html (1108 words)

	Genetics Program at Michigan State University
		Our research is directed primarily toward plant-virus interactions, specifically potyvirus infection of cucurbit crops.
		From the host side we are genetically and physiologically characterizing naturally occurring potyvirus resistance in cucumber; from the virus side we are investigating viral domains that influence host range and/or trigger host defense responses.
		We are also identifying and characterizing host proteins that directly interact with viral proteins and are studying the interaction between viral RNA-dependent RNA polymerase and host poly- (A) -binding protein.
	www.ns.msu.edu /genetics/faculty/grumet.html (355 words)

	New Disease Reports - First report of Bean common mosaic potyvirus in Western Australia
		Extracts of symptomatic leaf samples tested positive with generic potyvirus monoclonal antibody in ELISA and infected Chenopodium quonoa, C.
		For molecular identification, total RNA was isolated from symptomatic leaf tissues using an RNeasy Plant Mini Kit (Qiagen).
		The RNA samples were tested using RT-PCR and generic potyvirus specific primers that amplify a 1700 bp fragment from the 3’ end of the genome [5’GTTTTCCCAGTCACGA C(T) ; 5’GGNAAYAAYAGYGGNCARCC] (Chen et al., 2001).
	www.bspp.org.uk /ndr/jan2005/2005-03.asp (462 words)

	[No title]
		Symptoms; Fruit symptoms of plum pox potyvirus infection on apricot.
		Symptoms; Fruit symptoms of plum pox potyvirus infection on plum.
		Symptoms; Leaf symptoms of plum pox potyvirus infection on plum cv.
	www.invasive.org /browse/subject.cfm?sub=11223 (196 words)

	PestTracker Invasive Pathogen: Plum Pox, Potyvirus Group (PPV)
		PestTracker Invasive Pathogen: Plum Pox, Potyvirus Group (PPV)
		Penn State University, Fruit Research and Extension Center, Biglerville, Pa., for making these images available.
		Plum Pox Potyvirus Disease of Stone Fruits [APS.net]
	ceris.purdue.edu /napis/pests/ppox/index.html (263 words)

	Re: Cypripedium Potyvirus (Site not responding. Last check: 2007-10-29)
		I can't say if this is a potyvirus, as I have never had it myself (lucky me!) but I would try to have it tested.
		A virus is systemic and can only be killed if you kill every infected cell and for a virus that means the whole plant including the rhizome...
		: I can't say if this is a potyvirus, as I have never had it myself (lucky me!) but I would try to have it tested.
	www.cypripedium.de /forum/messages/2213.html (248 words)

	(WO/1999/051749) RECOMBINANT POTYVIRUS CONSTRUCT AND USE THEREOF (Site not responding. Last check: 2007-10-29)
		The present invention relates to a recombinant potyvirus infectious nucleic acid, preferably cDNA or RNA, construct useful for plant cross protection, comprising a full length clone characterized in that its HC-Pro gene conserved FRNK box sequence contains a substitution, preferably a substitution of Arg.
		The present invention further relates to a method for the production of a mild strain of potyvirus utilizing the above mentioned construct and to a method for protecting plants from viral infection and for transient expression of foreign nucleic acid (genes) in plants, using said construct.
		The present invention also relates to a method for providing protection against viral infection in plants comprising inoculating plants, by mechanical inoculation or by bombardment, with the recombinant potyvirus infectious construct.
	www.wipo.int /pctdb/en/wo.jsp?KEY=99/51749.991209 (314 words)

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