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Topic: Primase

Related Topics

Helicase

Lagging strand

RNA primer

DNA replication

DNA polymerase

	NationMaster - Encyclopedia: Primase
		Primase is activated by DNA helicase where it then synthesizes a short RNA primer approximately 11 ±1 nucleotides long, to which new nucleotides can be added by DNA polymerase.
		Primase is of key importance in DNA replication because no known DNA polymerases can initiate the synthesis of a DNA strand without initial RNA primers.
		The stability of this primer elongation complex is measured by challenging the protein-DNA complex with exonuclease III and monitoring the degradation of the radiolabeled primer strand as the complex dissociates.
	www.nationmaster.com /encyclopedia/Primase (591 words)

	Primase - Wikipedia, the free encyclopedia
		Primase is activated by DNA helicase where it then synthesizes a short RNA primer approximately 11 ±1 nucleotides long, to which new nucleotides can be added by DNA polymerase.
		Primase is of key importance in DNA replication because no known DNA polymerases can initiate the synthesis of a DNA strand without initial RNA primers.
		A primer is a short RNA segment that is complementary to a DNA segment, and is necessary to begin DNA replication.
	en.wikipedia.org /wiki/Primase (148 words)

	BACTERIAL PRIMASE HOME PAGE
		Primase is required because DNA polymerases cannot initiate polymer synthesis; DNA polymerases can only elongate polymers that have already been initiated.
		The sequences of the six primase ts mutations have been determined and there are good hints as to how some of these mutations alter the biochemistry of primase.
		The bacterial primases motif is the hinge polypeptide between subdomains 1 and 2 and there is a Hydrogen bond between Arg146 from motif 2 in sub1 and Asp347 from motif 6 in sub2 may stabilize the subdomain 1-2 interface.
	chem-mgriep2.unl.edu /replic/Primase.html (1698 words)

	DNA Primase
		DNA primases catalyse the synthesis of the short RNA primers on single stranded (ss) DNA templates that are used by DNA polymerase to initiate the synthesis of Okazaki fragments on the lagging strand.
		Primase is an essential enzyme in all well characterised systems of DNA replication.
		This primase is active in a general priming assay (K. Marians, unpublished data).
	sci.cancerresearchuk.org /labs/wigley/projects/primase/primase.html (572 words)

	Conditional Mutations in the Yeast DNA Primase Genes Affect Different Aspects of DNA Metabolism and Interactions in the ...
		Conditional Mutations in the Yeast DNA Primase Genes Affect Different Aspects of DNA Metabolism and Interactions in the DNA Polymerase {alpha}-Primase Complex -- Longhese et al.
		Conditional Mutations in the Yeast DNA Primase Genes Affect Different Aspects of DNA Metabolism and Interactions in the DNA Polymerase {alpha}-Primase Complex
		Identification of the nuclear localization signal of mouse DNA primase: nuclear transport of p46 subunit is facilitated by interaction with p54 subunit
	www.genetics.org /cgi/content/abstract/133/2/183 (499 words)

	Pfam 19.0 : DNA_primase_S (Site not responding. Last check: 2007-10-30)
		DNA primase synthesises the RNA primers for the Okazaki fragments in lagging strand DNA synthesis.
		DNA primase PUBMED:2023935 synthesizes the RNA primers for the Okazakifragments in lagging strand DNA synthesis.
		DNA primase is a heterodimer of large (p60) and small (p50) subunits in eukaryotes.
	pfam.wustl.edu /cgi-bin/getdesc?name=DNA_primase_S (173 words)

	DNA Primase
		DNA primases catalyse the synthesis of the short RNA primers on single stranded (ss) DNA templates that are used by DNA polymerase to initiate the synthesis of Okazaki fragments on the lagging strand.
		Primase is an essential enzyme in all well characterised systems of DNA replication.
		Two of the other motifs were proposed to be involved in Mg -mediated NTP binding motif, the motifs are all within the central part of the primase sequence, which probably constitutes the catalytic core of the enzyme.
	www.icnet.uk /labs/wigley/projects/primase/primase.html (572 words)

	Mechanism and Stoichiometry of Interaction of DnaG Primase with DnaB Helicase of Escherichia coli in RNA Primer ... (Site not responding. Last check: 2007-10-30)
		Primase initiates synthesis of the RNA primers (18-22).
		of primase in the presence (lanes 2, 4, 6, and 8) or in the absence (lanes 1, 3, 5, and 7) of 0.2 µ
		of primase in the absence (lane 1) or in the presence (lanes 2-6) of 0.1-0.3 µ
	intl.jbc.org /cgi/content/full/278/52/52253 (5435 words)

	An N-terminal fragment of the gene 4 helicase/primase of bacteriophage T7 retains primase activity in the absence of ...
		An N-terminal fragment of the gene 4 helicase/primase of bacteriophage T7 retains primase activity in the absence of helicase activity -- Frick et al.
		An N-terminal fragment of the gene 4 helicase/primase of bacteriophage T7 retains primase activity in the absence of helicase activity
		Primase and helicase activities of bacteriophage T7 are present in a single polypeptide coded by gene 4.
	www.pnas.org /cgi/content/short/95/14/7957 (749 words)

	Ask A Scientist - Telomeres and primase
		In DNA replication, the lagging strand is initiated by DNA primase, which synthesizes a short RNA oligonucleotide, which is then extended by DNA polymerase.
		At the very end of the chromosome, even if primase could initiate RNA primer synthesis from the first available nucleotide, there is no mechanism to replace this RNA primer with new DNA, since there is no upstream 3' end of DNA.
		The length of the primer made by primase is between 7 and 10 nucleotides, which corresponds to the length of 1 to 2 telomeric repeats.
	www.hhmi.org /cgi-bin/askascientist/highlight.pl?kw=&file=answers/genetics/ans_035.html (612 words)

	Identification and subcellular localization of the polypeptide for chick DNA primase with a specific monoclonal ...
		Identification and subcellular localization of the polypeptide for chick DNA primase with a specific monoclonal antibody -- Hirose et al.
		Identification and subcellular localization of the polypeptide for chick DNA primase with a specific monoclonal antibody
		in serum-deprived cultures, the expression of DNA primase and its
	www.jbc.org /cgi/content/abstract/263/6/2925 (367 words)

	DigitalCommons@University of Nebraska - Lincoln \| Primase structure and function
		Primase is the ssDNA-dependent RNA polymerase that synthesizes RNA primers during DNA replication.
		Primase has “RNAP” motif, a sequence found in all RNA polymerases which may be involved in chain initiation.
		Using artificial ssDNA templates, primase has been shown to be the slowest and most error-prone polymerase yet studied.
	digitalcommons.unl.edu /chemfacpub/2 (308 words)

	Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural ...
		primases in the archaeo-eukaryotic and bacterial lineages is
		primases, ORF7 and ORF49, are fused to a UL9-like helicase of
		the TOPRIM primase is fused to a UL9 helicase, whereas in the
	nar.oxfordjournals.org /cgi/content/full/33/12/3875 (9691 words)

	p12DOC-1, a growth suppressor, associates with DNA polymerase {alpha}/primase -- MATSUO et al. 14 (10): 1318 -- The ...
		:primase is the principal polymerase in eukaryotic DNA replication.
		:primase inhibitor aphidicolin (30 µg/ml) and absence of pol-
		:primase, the principal enzyme for DNA replication, is composed of
	www.fasebj.org /cgi/content/full/14/10/1318 (3109 words)

	BioMed Central \| Full text \| Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected ...
		The chromatin binding and sub-nuclear distribution of DNA primase catalytic subunit (Spp1) is affected by Cdc23 cleavage and also by inactivation of Cdc23 using a degron allele, implying that DNA polymerase α-primase function is dependent on Cdc23.
		In contrast to the effect on Spp1, the chromatin association of the Psf2 subunit of the GINS complex is not affected by Cdc23 inactivation.
		An earlier study has shown that the primase catalytic subunit, Spp1, is functional when C-terminally tagged with GFP and nuclear throughout the cell cycle [36].
	www.biomedcentral.com /1471-2199/6/13 (5590 words)

	Pfam 19.0 : DNA_primase_lrg (Site not responding. Last check: 2007-10-30)
		DNA primase is the polymerase that synthesises small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
		DNA primase is a heterodimer of two subunits, the small subunit Pri1 (48 kDa in yeast), and the large subunit Pri2 (58 kDa in the yeast S. cerevisiae) [1].
		DNA primase is a heterodimer of two subunits, the small subunit Pri1 (48 kDa in yeast), and the large subunit Pri2 (58 kDa in the yeast Saccharomyces cerevisiae) PUBMED:2528682.
	pfam.wustl.edu /cgi-bin/getdesc?name=DNA_primase_lrg (241 words)

	Mouse DNA Primase, p58 Antibodies,
		In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers.
		The eukaryotic primase is composed of two subunits, p49 (human 420 aa; chromosome 12q13) and p58 (human 509 aa, chromosome 6p12-p11.1), which purify as a complex tightly bound to DNA polymerase alpha.
		Primase initiates synthesis with a triphosphate purine moiety at the 5'-end.
	www.4adi.com /data/dna/dnpr14.html (342 words)

	G Nigel Godson Ph.D., D.Sc.
		We are studying the structure and mechanism of action of Escherichia coli primase and human primase.
		We are developing evidence for a model of action of Escherichia coli primase that we think is applicable to all primases.
		To support this model, we are studying the structure of primase and its interaction with the template DNA.
	www.med.nyu.edu /Research/G.Godson-res.html (267 words)

	University of Colorado at Boulder :: Chemistry and Biochemistry :: R. Kuchta
		Presently, we are studying the cellular DNA polymerase alpha-primase complex, an enzyme complex required for the initiation of all new strands of DNA during S phase, and Herpes DNA primase, an enzyme required for initiating new strands of Herpes DNA in virally infected cells.
		Other questions of interest include the arrangement of the primase and polymerase active sites, how the polymerase and primase communicate with and affect each other's activity, and the functional significance of the homology between the cellular primase and DNA polymerase beta.
		Kirk, B. and Kuchta, R. (1999) "Arg304 of Human DNA Primase Is a Key Contributor to Catalysis and NTP Binding: Primase and the Family X Polymerases Share Significant Sequence Homology" Biochemistry, 38, 7727-7736.
	www.colorado.edu /chem/DEC/people/kuchtar.html (1040 words)

	Herpes simplex virus helicase-primase inhibitors are active in animal models of human disease - Nature Medicine
		Inhibition of the enzymatic activities was through stabilization of the interaction between the helicase-primase and DNA substrates, preventing the progression through helicase or primase catalytic cycles.
		Primase assays were performed with enzyme containing UL5(K103A), increasing priming activity 15-fold.
		M biotinylated UTP, 100 nM of the 51-mer primase template and 160 nM of the UL5(K103A) helicase-primase.
	www.nature.com /nm/journal/v8/n4/full/nm0402-386.html (4147 words)

	Misincorporation of nucleotides by calf thymus DNA primase and elongation of primers containing multiple noncognate ...
		Misincorporation of nucleotides by calf thymus DNA primase and elongation of primers containing multiple noncognate nucleotides by DNA polymerase alpha -- Sheaff and Kuchta 269 (30): 19225 -- Journal of Biological Chemistry
		Misincorporation of nucleotides by calf thymus DNA primase and elongation of primers containing multiple noncognate nucleotides by DNA polymerase alpha
		Misincorporation of nucleotides by calf thymus DNA primase was examined
	www.jbc.org /cgi/content/abstract/269/30/19225 (342 words)

	[No title] (Site not responding. Last check: 2007-10-30)
		204 : Q730P2_BACC1 0.38 0.51 79 311 198 427 230 1 3 571 Q730P2 DNA primase (EC 2.7.7.-).
		207 : Q71IC5_LACDL 0.38 0.48 79 247 90 258 167 2 4 261 Q71IC5 DNA primase (EC 2.7.7.-) (Fragment).
		233 : Q72J83_THET2 0.36 0.41 79 309 183 411 227 4 6 590 Q72J83 DNA primase (EC 2.7.7.-).
	srs.embl-heidelberg.de:8000 /srs5bin/cgi-bin/wgetz?-e+[HSSP-ID:'1dde'] (6041 words)

	University of Nebraska-Lincoln - Scarlets Web - 6/10/99
		The stumbling ballerina, in scientific terms, is primase, a special kind of RNA polymerase; an enzyme that initiates, or primes, DNA synthesis by initiating the synthesis of nucleic acid polymers, the building blocks of the replicated strand.
		He says that the errors that primase makes could be an important factor in natural mutagenesis, the process by which mutant genes are formed.
		By learning how primase works, it may be possible to develop drugs that act against primase so that it will kill the bacterium within which it acts and prevent that bacterium from mutating into a drug-resistant strain.
	www.unl.edu /scarlet/v9n17/v9n17features.html (4212 words)

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