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Topic: Protein electrophoresis

Related Topics

Serum protein electrophoresis

Blood proteins

Gel electrophoresis

Paraprotein

Agarose gel electrophoresis

Capillary electrophoresis

Albumin

Western blot

Protein

Serum

DNA electrophoresis

Immunoglobulin

Blood test

Blood

Molecular biology

	Serum protein electrophoresis - Wikipedia, the free encyclopedia
		Serum protein electrophoresis is a laboratory test that examines specific proteins in the blood called globulins.
		Increased beta-1 protein is typical of iron deficient anaemia, pregnancy, and oestrogen therapy.
		C reactive protein can be seen as a faint band when the level is grossly abnormal, e.g.
	en.wikipedia.org /wiki/Serum_protein_electrophoresis (833 words)

	1-01 Newsletter
		Protein electrophoresis is a practical and useful test in assessing health status of psittacine birds.
		It serves as the major reservoir of protein, is a main contributor of colloidal osmotic pressure, is involved in acid-base balance, and acts as a transport carrier for small molecules such as vitamins, minerals, hormones, fatty acids, and many drugs.
		Pre-albumin is a separate and distinct plasma protein fraction that precedes albumin on electrophoresis, and is involved in the transportation of thyroid hormones.
	www.antechdiagnostics.com /clients/antechNews/2001/1-01.htm (1150 words)

	American Family Physician: Understanding and interpreting serum protein electrophoresis
		Serum serum protein electrophoresis is a laboratory examination that commonly is used to identify patients with multiple myeloma and other disorders of protein.
		The subsets of these proteins and their relative quantity are the primary focus of the interpretation of serum protein electrophoresis.
		An M protein is characterized by the presence of a sharp, well-defined band with a single heavy chain and a similar band with a kappa or lambda light chain.
	www.findarticles.com /p/articles/mi_m3225/is_1_71/ai_n8702975 (1280 words)

	Protein Electrophoresis
		To compare proteins from two different types of plants, scientists sometimes carry out an experiment to separate the proteins from each other, so they can be examined separately (a protein electrophoresis experiment).
		A mixture of protein molecules is extracted from plant tissue and placed at one end of a slab of gelatin-like material.
		All of the proteins are pulled toward the positive pole of the electric field (+ in each of the three gel pictures), but because big molecules travel slowly through the gel matrix and small molecules travel more quickly, the proteins become separated according to their size.
	gslc.genetics.utah.edu /units/basics/conservation/tools/protein.cfm (342 words)

	Gale Encyclopedia of Medicine: Protein electrophoresis
		Protein electrophoresis is used to evaluate, diagnose and monitor a variety of diseases and conditions.
		Protein electrophoresis is used to determine the total amount of protein in the blood, and to establish the levels of other types of proteins called albumin, alpha
		Protein electrophoresis is performed on urine samples to classify kidney disorders that cause protein loss.
	www.findarticles.com /p/articles/mi_g2601/is_0011/ai_2601001128 (1104 words)

	Protein gel electrophoresis
		In a gel of uniform density the relative migration distance of a protein (Rf, the f as a subscript) is negatively proportional to the log of its mass.
		If proteins of known mass are run simultaneously with the unknowns, the relationship between Rf and mass can be plotted, and the masses of unknown proteins estimated.
		Protein separation by SDS-PAGE can be used to estimate relative molecular mass, to determine the relative abundance of major proteins in a sample, and to determine the distribution of proteins among fractions.
	www.ruf.rice.edu /~bioslabs/studies/sds-page/gellab2.html (665 words)

	Protein quantitation and electrophoresis
		Electrophoresis is a method for separating molecules in solution based on differences in their charge, size and/or shape.
		Proteins of known molecular weight are electrophoresed alongside with polypeptides of unknown molecular weight.
		The standard curve for electrophoresis is constructed by plotting the log of molecular weight of known proteins (y-axis) versus the distance migrated (x-axis) measured from the sample well.
	www.cas.vanderbilt.edu /bsci111a/protein-electro/supplemental.htm (1453 words)

	Electrophoresis: The Test
		Electrophoresis is used to identify the presence or absence of aberrant proteins and to identify when different groups of proteins are increased or decreased in serum or urine.
		Electrophoresis is not usually necessary to assess the loss of small to moderate amounts of protein due to temporary conditions, such as a urinary tract infection or an acute inflammation.
		Protein electrophoresis may be ordered when a doctor is investigating symptoms that suggest multiple myeloma, such as bone pain, anemia, fatigue, unexplained fractures, and recurrent infections.
	www.labtestsonline.org /understanding/analytes/electrophoresis/test.html (916 words)

	Protein Electrophoresis \| aHealthyAdvantage (Site not responding. Last check: 2007-10-08)
		Electrophoresis is a technique used to separate different elements (fractions) of a blood sample into individual components.
		If serum protein electrophoresis has demonstrated a significant rise at the gammaglobulin level, immunoelectrophoresis is done to identify the specific globulin that is involved.
		A group of proteins in blood plasma whose levels can be measured by electrophoresis in order to diagnose or monitor a variety of serious illnesses.
	www.ahealthyadvantage.com /topic/topic100587356 (1087 words)

	Clinical Pathology Section -- NYS Animal Health Diagnostic Laboratory (Site not responding. Last check: 2007-10-08)
		Electrophoresis is indicated for determination of the underlying nature of a hyperglobulinemia or if multiple myeloma is suspected in a patient.
		Electrophoresis can be performed on urine, but similar to CSF, this requires concentrating because protein values are usually quite low in urine (we need a minimum of 600 mg/dL of protein to perform electrophoresis).
		Electrophoresis on urine is the preferred technique for identification of Bence Jones proteins in patients with suspected multiple myeloma.
	www.diaglab.vet.cornell.edu /clinpath/test/immun/electro.asp (557 words)

	Protein electrophoresis - Wikipedia, the free encyclopedia
		In chemistry and medicine, protein electrophoresis is a method of analysing a mixture of proteins by means of gel electrophoresis, mainly in blood serum (blood plasma is not suitable).
		There are two large classes of blood proteins: albumin and globulin.
		They are generally equal in proportion, but albumin is much smaller and lightly negatively charged, leading to an accumulation of albumin on the electrophoretic gel.
	en.wikipedia.org /wiki/Protein_electrophoresis (179 words)

	Protein Electrophoresis, 24-Hour Urine
		A serum protein electrophoresis should be reviewed concurrently if one has not been recently studied.
		In nonselective glomerular proteinuria, the urine electrophoretic pattern is often a nonspecific one which may be called "mirror image" to that of the serum.
		proteins, and transferrin are the predominant proteins identified on the urine protein electrophoresis, with a relative absence of heavier molecular weight proteins (ie, alpha
	www.labcorp.com /datasets/labcorp/html/chapter/mono/sc018900.htm (309 words)

	Serum Protein Electrophoresis (SPE): Test Overview (Site not responding. Last check: 2007-10-08)
		Proteins are substances made up of smaller building blocks called amino acids.
		Proteins carry a positive or a negative electrical charge, and they move in fluid when placed in an electrical field.
		Serum protein electrophoresis uses an electrical field to separate the proteins in the blood serum into groups of similar size, shape, and charge.
	www.meritcare.com /hwdb/showtopic.aspx?pd_hwid=hw43653 (305 words)

	REP High Resolution Protein Electrophoresis
		High Resolution electrophoresis is the method of choice for detecting monoclonal immunoglobulins in serum and urine.
		The superior resolution and sensitivity separates and detects proteins with similar electrophoretic mobilities and those present in low concentrations that may be invisible on other methods.
		High resolution electrophoresis is excellent for visualization of these multiple restricted bands and may help rule out multiple sclerosis as the basis for neurological dysfunction.
	www.helena.com /catalog/rephires.htm (238 words)

	Thermo Electron Corporation - - Proteomics - Gel Electrophoresis Introduction
		Electrophoresis is the process whereby ions move through a medium in response to an applied electric field.
		Separation of ions by electrophoresis exploits the fact that the rate of motion of charged particles in any particular applied electric field is directly proportional to their charge and inversely proportional to their size and the viscosity of the medium.
		Methodologies employed for the isolation of proteins from gels and subsequent processing for mass spectrometric analysis are dependent on the source of the sample, the desired end result and nature of the target protein.
	www.thermo.com /com/cda/resources/resources_detail/1,,35,00.html (739 words)

	REP Protein Electrophoresis
		As an indicator of liver and kidney disease, immune deficiencies, malignancies of the immune system, acute and chronic infection, genetic deficiencies, central nervous system disease and numerous other pathologies, serum protein electrophoresis exceeds any other single test in its ability to detect so broad a spectrum of disease states.
		The Serum Protein Diagnostic Program, an integral function, can be activated to automatically interpret protein patterns based on scanned data for fractional values, total protein, patient age and pattern tracing characteristics.
		Protein bands are stained with Ponceau S or Acid Blue and quantitated by densitometry.
	www.helena.com /catalog/repserumprotein.htm (189 words)

	Serum Protein Electrophoresis (SPE) - [Medical Test] - Quest Diagnostics Patient Health Library (Site not responding. Last check: 2007-10-08)
		Electrophoresis on protein in urine may also be done, especially if the results of the serum protein electrophoresis test are abnormal.
		Normally very little protein is found in urine, but certain diseases (such as multiple myeloma) cause large amounts of protein to leak into the urine.
		Although abnormal protein levels may be found in many conditions (such as kidney disease, chronic liver disease, systemic lupus erythematosus, rheumatoid arthritis, or leprosy), serum protein electrophoresis is usually not done to diagnose these conditions.
	www.questdiagnostics.com /kbase/topic/medtest/hw43650/consider.htm (215 words)

	Electrophoresis: The Test Sample (Site not responding. Last check: 2007-10-08)
		Protein electrophoresis is a method for separating the proteins found in blood (serum) or urine.
		These separated proteins may be detected by the use of a dye that binds to (stains) all of the proteins and reveals a characteristic pattern of bands.
		Specific proteins of interest can be identified by first mixing them into the gel with antibodies, then washing away all the other proteins prior to staining.
	www.labtestsonline.org.uk /understanding/analytes/electrophoresis/sample.html (590 words)

	Protein Electrophoresis: PageRuler™ Prestained Protein Ladder
		PageRuler™ Prestained Protein Ladders (PageRuler™ Prestained Protein Ladder Plus and PageRuler™ Prestained Protein Ladder) are designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency and approximate sizing of proteins (1-3).
		Each lot of PageRuler™ Prestained Protein Ladder is calibrated against a precisely sized PageRuler™ Unstained Protein Ladder (#SM0661) in Tris-glycine gel and the calculated apparent molecular weights are reported in the product’s Certificate of Analysis.
		Burnette, W.N., “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal Biochem., 112 (2), 195-203, 1981.
	www.fermentas.com /catalog/electrophoresis/marksm0671.htm (440 words)

	Protein Staining Reagents for Protein Electrophoresis (Site not responding. Last check: 2007-10-08)
		ProteoProfile PTM Marker (P 1745), containing glycosylated and non–glycosylated proteins, was separated by electrophoresis on a 4–20% SDS–PAGE gel.
		Proteins can be easily destained with 25% (v/v) isopropanol and 10% (v/v) acetic acid for further analysis.
		Ponceau S (sodium salt) may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or PVDF membranes (Western blotting), as well as on cellulose acetate membranes.
	www.sigmaaldrich.com /Area_of_Interest/Life_Science/Proteomics_and_Protein_Expr/Protein_Analysis/Protein_Electrophoresis/Additional_Protein_Staining_Reagents.html (813 words)

	Protein Electrophoresis, Cellulose Acetate
		Electrophoresis is used to identify and study charged molecules and macromolecules.
		Standard proteins will be available in the laboratory, and are identified by name, known molecular weight, pI and the net charge each carries at the pH of the electrophoresis buffer we are using.
		Serum proteins are run for 45 minutes at 2 ma/strip and 300-350V (at no time should current exceed 3 ma/strip or the voltage exceed 450V!).
	employees.oneonta.edu /helsertl/ProtElectro.html (1746 words)

	Colorful Electrophoresis
		Electrophoresis is a standard molecular biological technique for separating nucleic acids and proteins by size.
		While the materials needed for nucleic acid or protein electrophoresis are expensive and may involve the handling of toxic chemicals, Kitchen Electrophoresis provides a safe, inexpensive and rapid method for demonstrating the principles of this important technique.
		In Kitchen Electrophoresis, agar agar (obtained from a health food store or Asian market) replaces agarose for the gel, a weak solution of table salt and water is used instead of a buffer solution, and liquid food colors or scientific stains simulate DNA, RNA or protein samples.
	gslc.genetics.utah.edu /units/activities/electrophoresis (891 words)

	Somagen Diagnostics Inc - Chemistry - Protein Electrophoresis
		Proteins are detected directly at a wavelength of 200nm eliminating the need for staining while providing a high degree of precision and accuracy.
		The concentrator you choose becomes as important as the assay that is being used to detect the proteins.
		If you have a protein that you are trying to capture, you want to ensure that the pore size of the membrane is small enough that you are not losing valuable protein that you are indeed, trying to ultimately detect and measure.
	www.somagen.com /html/chemistry/chem_protein.htm (1101 words)

	Teacher Guide: Muscle Protein Electrophoresis Experiment
		The process of electrophoresis involves an oxidation/reduction reaction (formation of H2 gas at the negative electrode and O2 gas at the positive electrode) that alters the pH at each electrode.
		The SDS is a detergent that denatures and sticks to proteins, keeping them unfolded and negatively charged during electrophoresis.
		Once the protein samples are loaded, you will not be able to determine which is which.
	biotech.biology.arizona.edu /labs/Muscle_teach.html (1197 words)

	2D Gel Electrophoresis, Proteomics, Protein Characterization
		High abundance proteins including albumins and immunoglobins are removed from serum and plasma prior to electrophoresis.
		Selection of proteins can also be achieved by studying individual cellular compartments (organelles) purified by differential centrifugation.
		Depending on the protein(s) of interest, 20 % gels are used to resolve small proteins, 12 % gels to resolve mid-range proteins, and 7.5% gels for large proteins.
	www.paragonbioservices.com /Proteomics/electrophoresis.htm (290 words)

	ProteomeLab™ PA 800 Protein Characterization System -- Protein Electrophoresis (Site not responding. Last check: 2007-10-08)
		The ProteomeLab PA 800 resolves and quantifies proteins by their isoelectric point and molecular weight, generates high-resolution peptide maps and carbohydrate profiles and provides front-end separation to mass spectrometry.
		And the separation is by capillary gel electrophoresis, using a formulation of polymers optimized for the resolution of proteins with a wide molecular weight range.
		The distribution of glycans released from a glycoprotein yields a fingerprint useful in protein identification.
	www.beckman.com /products/instrument/protein/proteomelab_pa800_dcr.asp (1070 words)

	LabPlus Handbook (Site not responding. Last check: 2007-10-08)
		Sometimes a myeloma may produce light chains only, in which case the serum protein electrophoresis may be normal.
		Urine Protein Electrophoresis: a very small amount ("faint trace") of albumin is not abnormal, particularly after the laboratory has concentrated the urine prior to performing the electrophoresis.
		The additional presence of other protein fractions indicates that the glomeruli are significantly effected; sufficient to allow the larger proteins to filter through.
	www.adhb.govt.nz /LabPlusHandbook/handbook/ManualComments__Protein_Electrophoresi.htm (737 words)

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