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Topic: Protein splicing

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G protein coupled receptors

Protein data bank

G protein

protein folding

protein biosynthesis

Protein kinases

protein synthesis

protein structure prediction

integral membrane protein

subunit proteins

C reactive protein

protein electrophoresis

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	C&EN: TODAY'S HEADLINES - CONTROLLED PROTEIN SPLICING
		The compounds do this by initiating protein splicing--a process in which the primary sequence of a precursor protein is edited by removal of an internal segment.
		In the new technique, each of two protein fragments is derivatized with a protein-splicing construct--one-half of an "intein" linked to a protein that can bind a dimerizer ligand.
		An intein is an intervening protein domain that pops spontaneously out of any protein in which it's found, causing the flanking protein fragments (exteins) to become joined through a conventional peptide bond.
	pubs.acs.org /cen/topstory/8029/8029notw4.html (503 words)

	Intein (Site not responding. Last check: 2007-10-15)
		An intein is a segment of a protein that is able to excise itself and rejoin the remaining portions (the exteins) with a peptide bond.
		Inteins are very efficient at protein splicing and they have accordingly found an important role in biotechnology.
		Inteins have been engineered for particular applications such as protein synthesis, and the selective labeling of protein segments, which is useful for NMR studies of large proteins.
	www.sciencedaily.com /encyclopedia/intein (481 words)

	Understanding and Exploiting Protein Splicing (Site not responding. Last check: 2007-10-15)
		Protein splicing is a remarkable self-catalyzed post-translational process in which nonfunctional polypeptides undergo highly specific rearrangements to yield functional proteins.
		The process involves the excision of an internal protein (intein) and ligation of the flanking external proteins (exteins) to form a mature protein.
		Since the discovery of "protein introns" nearly a decade ago, considerable research has been carried out to determine the mechanism, function, and origin of protein splicing.
	www.chem.umn.edu /studsem/abstract9900/beyer02.15.00/abstract.html (118 words)

	Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein -- Southworth and Perler 184 (22): 6387 -- The ...
		Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein -- Southworth and Perler 184 (22): 6387 -- The Journal of Bacteriology
		Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein
		Shown are results for the control MP protein (lane 1), soluble cell extract (lane 2), flowthrough after affinity chromatography over amylose resin (lane 3), eluate from amylose column (lane 4), Genenase I-cleaved eluate (lane 5), and a broad-range protein marker (New England Biolabs) (lane 6) on a sodium dodecyl sulfate-polyacrylamide gel stained with Coomassie blue.
	jb.asm.org /cgi/content/full/184/22/6387 (1034 words)

	Protein Splicing Bibliography (Site not responding. Last check: 2007-10-15)
		Chen, L., Benner, J. and Perler, F.B. (2000) Protein splicing in the absence of an intein penultimate histidine.
		Evans, T.C. and Xu, M.Q. (2002) Mechanistic and kinetic considerations of protein splicing.
		Lew, B.M., Mills, K.V. and Paulus, H. Characteristics of protein splicing in trans mediated by a semisynthetic split intein.
	www.neb.com /inteins/int_bibliog.html (7893 words)

	Regulation of Alternative Splicing of CD45 by Antagonistic Effects of SR Protein Splicing Factors -- ten Dam et al. 164 ...
		Inhibition by SR proteins of splicing of a regulated adenovirus pre-mRNA.
		Splicing efficiency of human immunodeficiency virus type 1 tat RNA is determined by both a suboptimal 3' splice site and a 10 nucleotide exon splicing silencer element located within tat exon.
		The polypyrimidine tract binding protein binds upstream of neural cell-specific c-src exon N1 to repress the splicing of the intron downstream.
	www.jimmunol.org /cgi/content/full/164/10/5287 (5574 words)

	Identification and Characterization of a Novel Serine-Arginine-Rich Splicing Regulatory Protein -- Barnard and Patton ...
		The human and rat proteins are 86% identical, with the exception of a 16-amino-acid insertion in the human protein.
		Splicing extracts were incubated with the various resins, and flowthrough extracts were used in splicing reactions alone (lanes 1 to 3) or with the addition of purified SR proteins (lane 4).
		A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA.
	mcb.asm.org /cgi/content/full/20/9/3049 (7157 words)

	Protein Splicing and Auto-cleavage of Bacterial Intein-like Domains Lacking a C'-flanking Nucleophilic Residue -- Dassa ...
		Protein Splicing and Auto-cleavage of Bacterial Intein-like Domains Lacking a C'-flanking Nucleophilic Residue -- Dassa et al.
		Proteins were separated on SDS-PAGE and either stained with Coomassie Blue or detected by anti-M, anti-C, or anti-GroEL (Anti-G) antibodies.
		Proteins were separated on SDS-PAGE and either stained with Coomassie Blue or detected by anti-M, anti-C, or anti DnaK antibodies.
	www.jbc.org /cgi/content/full/279/31/32001 (4414 words)

	The adenovirus E4-ORF4 splicing enhancer protein interacts with a subset of phosphorylated SR proteins -- Estmer ... (Site not responding. Last check: 2007-10-15)
		with a subset of HeLa SR proteins: SF2/ASF and SRp30c.
		Kanopka,A., Mühlemann,O. and Akusjärvi,G. (1996) Inhibition by SR proteins of splicing of a regulated adenovirus pre-mRNA.
		Roscigno,R.F. and Garcia-Blanco,M.A. (1995) SR proteins escort the U4/U6•U5 tri-snRNP to the spliceosome.
	embojournal.npgjournals.com /cgi/content/full/20/4/864 (5202 words)

	The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and ... (Site not responding. Last check: 2007-10-15)
		Splicing reactions were performed and analysed as in Figure 1A, with the 20 pmol of recombinant SRp30c added to the RNA substrate (IIIa) 5 min prior to addition of HeLa-NE.
		The indicated amounts of recombinant proteins were incubated with substrate RNA (IIIa) for 5 min prior to addition of HeLa-NE and complex analysis as in Figure 1E.
		Burnette, W.N. `Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal.
	embojournal.npgjournals.com /cgi/content/full/18/4/1014 (7134 words)

	Gene
		Thus, the structure of the DNA double helix had all the characteristics that were to be expected from a molecule serving as an autocatalytic hereditary entity (Chadarevian 2002).
		In more detail, the transfer of information from nucleic acid to nucleic acid, or from nucleic acid to protein may be possible, but transfer from protein to protein, or from protein to nucleic acid is impossible.
		A spliced messenger sometimes may comprise a fraction as little as ten percent or less of the primary transcript.
	plato.stanford.edu /entries/gene (9187 words)

	Using Protein Splicing (Site not responding. Last check: 2007-10-15)
		Nature had devised the scheme of protein splicing millions of years prior to its discovery in 1990, but since then, several groups have made incredible strides to use the system to advance science.
		Tom Muir's group has made significant use of protein splicing to study the Src homology (SH) domains of the Abelson non-receptor protein tyrosine kinase (Abl).
		The SH3 domain is also isolated by treatment of the fusion protein, still bound to the chitin beads, with thiophenol or DTT, trapping the C-terminal residue of the SH3 domain in a highly reactive thioester.
	udel.edu /~clined/UsingProteinSplicing.htm (369 words)

	Dalhousie Biochemistry Publications (Site not responding. Last check: 2007-10-15)
		Russell, A.G., Shutt, T.E., Watkins, R.F. and Gray, M.W. (2005) An ancient spliceosomal intron in the ribosomal protein L7a gene (Rpl7a) of Giardia lamblia.
		J.P. Wyles and N.D. Ridgway (2004) VAMP-associated protein regulates partitioning of oxysterol-binding protein-related protein -9 between the endoplasmic reticulum and Golgi apparatus.
		Yan, S.R., Al-Hertani, W., Byers, D., and Bortolussi, R. (2002) Lipopolysaccharide-binding protein- and CD14-dependent activation of mitogen-activated protein kinase p38 by lipopolysaccharide in human neutrophils is associated with priming of respiratory burst.
	www.biochem.dal.ca /publications/index.php (5189 words)

	A Conserved Drosophila Transportin-Serine/Arginine-rich (SR) Protein Permits Nuclear Import of Drosophila SR Protein ...
		A Conserved Drosophila Transportin-Serine/Arginine-rich (SR) Protein Permits Nuclear Import of Drosophila SR Protein Splicing Factors and Their Antagonist Repressor Splicing Factor 1 -- Allemand et al.
		Members of the highly conserved serine/arginine-rich (SR) protein family are nuclear factors involved in splicing of metazoan
		The SR Protein SC35 Is Responsible for Aberrant Splicing of the E1{alpha} Pyruvate Dehydrogenase mRNA in a Case of Mental Retardation with Lactic Acidosis
	www.molbiolcell.org /cgi/content/abstract/13/7/2436 (578 words)

	Pre-mRNA splicing in the absence of an SR protein RS domain -- Zhu and Krainer 14 (24): 3166 -- Genes and Development
		Pre-mRNA splicing in the absence of an SR protein RS domain -- Zhu and Krainer 14 (24): 3166 -- Genes and Development
		Pre-mRNA splicing in the absence of an SR protein RS domain
		SR proteins are essential pre-mRNA splicing factors that act at the earliest stages of splice-site recognition and spliceosome
	www.genesdev.org /cgi/content/abstract/14/24/3166 (690 words)

	Links -- Bioinformatics Core Facilities
		PKR - Protein kinase sequences, enzymology, genetics, and molecular and structural properties.
		Genome@home uses a computer algorithm (SPA), based on the physical and biochemical rules by which genes and proteins behave, to design new proteins (and hence new genes) that have not been found in nature.
		Protein Extensible Markup Language (PROXIML) - Problems associated with existing protein data formats, such as PDB and mmCIF, indicate a need for a more self-describing and machine-readable approach to exchanging protein-related data.
	www.biotech.unl.edu /bioinformatics/links.html (2470 words)

	Protein Splicing (Site not responding. Last check: 2007-10-15)
		This page will [soon] focus on the structural aspects involved in protein splicing.
		The Intein Database - brought to you by F.B. Perler of New England Biolabs
		Paulus, H. Protein Splicing and Related Forms of Protein Autoprocessing.
	www.stanford.edu /~gmartine/protein_splicing.html (83 words)

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