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Topic: Pseudomonas putida


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In the News (Thu 31 Dec 09)

  
  JGI Pseudomonas putida F1 Home   (Site not responding. Last check: 2007-11-06)
Pseudomonas putida F1 (Bacteria 6.2 Mb) is a versatile environmental isolate that is capable of growth on several aromatic hydrocarbons, including benzene, toluene, ethylbenzene and p -cymene.
Pseudomonas putida strains show optimal growth between 25-30?C and are easily isolated from soil and water by enrichment culture in mineral media with various carbon sources.
Pseudomonas putida F1 was isolated from a polluted creek in Urbana, IL by enrichment culture with ethylbenzene as the sole source of carbon and energy.
genome.jgi-psf.org /draft_microbes/psepu/psepu.home.html   (668 words)

  
 Pseudomonas.etc.   (Site not responding. Last check: 2007-11-06)
Generally, common to all constituent species of the genus Pseudomonas are certain physiological properties such as chemoorganotrophic nutrition, aerobic metabolism, absence of fermentation, absence of photosynthesis, inability to fix nitrogen, and capacity for growth at the expense of a large variety of organic substrates.
Pseudomonas species are renowned for their abilities to degrade compounds which are highly refractory to other organisms, including aliphatic and aromatic hydrocarbons, fatty acids, insecticides and other environmental pollutants.
Pseudomonas species, as well as species included in the newly-created genera Burkholderia and Ralstonia (ex-Pseudomonas) are among the most important bacteria that are pathogens of plants.
textbookofbacteriology.net /Pseudomonas.etc.html   (6220 words)

  
 Manganese Oxidation by Pseudomonas putida
Pseudomonas putida, as a species well-studied biochemically and genetically, should be useful as a model system for studying a mechanism of bacterial manganese oxidation.
Pseudomonas putida strains are best known to microbiologists for their ability to degrade aromatic hydrocarbons such as toluene, camphor, and naphthalene.
Pseudomonas putida has been recognized for many years as a common inhabitant of soils and fresh waters, and is described as "ubiquitous" by Palleroni (1992a).
genetics.med.harvard.edu /~depalma/mn/thesis.html   (15466 words)

  
 Pseudomonas - MicrobeWiki
Pseudomonas syringae pathovar tomato DC3000, the tomato and Arabidopsis thaliana pathogen, has genome 6.5 megabases in size that is compromised of a circular chromosome and two plasmids, which all encode for 5,763 ORFs.
Pseudomonas bacteria can be found in soil, marshes, coastal marine habitats, and plant and animal tissue; generally, these bacteria can tolerate a variety of physical conditions.
Pseudomonas aeruginosa is common in patients with compromised host defense mechanisms and is the "most common pathogen isolated from patients who have been hospitalized longer than one week" (Qarah and Cunha).
microbewiki.kenyon.edu /index.php/Pseudomonas   (773 words)

  
 Proline Catabolism by Pseudomonas putida: Cloning, Characterization, and Expression of the put Genes in the Presence of ...
putida KT2442 in vectors pLCR4, pLCR12, and pSLH4.
Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.
Espinosa-Urgel, M., Ramos, J.-L. Expression of a Pseudomonas putida Aminotransferase Involved in Lysine Catabolism Is Induced in the Rhizosphere.
jb.asm.org /cgi/content/full/182/1/91   (4636 words)

  
 U.S.-German research consortium sequences genome of versatile soil microbe
putida is a fast-growing bacterium that is found in most temperate soil and water habitats where oxygen is present.
putida was designated in 1982 by a National Institutes of Health advisory panel as the first "biosafety" host strain for gene cloning in Gram-negative soil bacteria.
The KT2440 strain of P. putida is used by researchers for the analysis and manipulation of genes from soil bacteria.
www.eurekalert.org /pub_releases/2002-12/tifg-urc120202.php   (949 words)

  
 STARVATION GENES, PROMOTERS AND STARVATION SURVIVAL FUSION MUTANTS OF PSEUDOMONAS PUTIDA
Pseudomonas putida ATCC 12633 was mutagenized by the suicide mini-Tn5::lacZ1 transcriptional fusion vector.
P putida MK1, used as the recipient strain for Tn5 mutagenesis, is a rifampicin-resistant derivative of P.
putida by conjugation in the presence of a helper plasmid.
www.isb.vt.edu /brarg/brasym94/kim.htm   (2185 words)

  
 Pseudo-Outbreak of Pseudomonas putida in a Hospital Outpatient Clinic Originating from a Contaminated Commercial ...
In July and August 2000, Pseudomonas putida was recovered from 10 specimens originating from a tertiary-care hospital's ear, nose, and throat (ENT) outpatient clinic.
putida colonization/infection continued to be identified in the microbiology laboratory, however, and a more thorough secondary environmental investigation was launched.
Pseudomonas putida: newly recognized pathogen in patients with cancer.
www.phac-aspc.gc.ca /publicat/ccdr-rmtc/00vol26/dr2621eb.html   (543 words)

  
 Pseudomonas putida - Wikipedia, the free encyclopedia
Pseudomonas putida is a flagellated rod-shaped bacterium that has the ability to degrade organic solvents.
putida is a safe strain of bacteria, unlike P.
This may be of use in the effective recycling of Polystyrene foam, otherwise thought to be non-biodegradable.
en.wikipedia.org /wiki/Pseudomonas_putida   (145 words)

  
 Pseudomonas Migula 1894, genus
[PSALLIDAS (P.G.) and PANAGOPOULOS (C.G.): A new bacteriosis of almond caused by Pseudomonas amygdali sp.
Pseudomonas balearica accomodates the ¤ Pseudomonas stutzeri genomovar 6 strains.
WOLTERINK (A.F.W.M.), JONKER (A.B.), KENGEN (S.W.M.) and STAMS (A.J.M.): Pseudomonas chloritidismutans sp.
www.bacterio.cict.fr /p/pseudomonas.html   (8519 words)

  
 BioMed Central | Full text | Display of a thermostable lipase on the surface of a solvent-resistant bacterium, ...
To express TliA on the surface of a solvent resistant bacterium, Pseudomonas putida GM730 [28], we used the same strategy to construct the INPNC-TliA and INP-TliA fusion proteins, the expression of which was directed from the surface display vectors pJHC13 and pJHC14.
We demonstrate the immobilization of a thermostable lipase from Pseudomonas fluorescens in an active form on the cell surface of Pseudomonas putida, a solvent-resistant bacterium, and show that the enzyme catalyzes reactions in the presence of an organic solvent.
An organic solvent-resistant bacterium, Pseudomonas putida GM730 [28], was employed for the surface immobilization of lipase and used for whole-cell biocatalysis in organic solvents.
www.biomedcentral.com /1472-6750/6/23   (4320 words)

  
 Pseudomonas Infection
Multi-drug resistant Pseudomonas is one of the most serious and difficult hospital-acquired infections to treat and affects patients with ventilator-associated pneumonia, cystic fibrosis, low white cell counts, burns and diabetic ulcers.
Pseudomonas aeruginosa and P maltophilia account for approximately 80 percent of pseudomonads recovered from clinical specimens.
Because of the frequency with which it is involved in human disease, P aeruginosa has received the most attention.
www.phageinternational.com /pathogens/pseudomonas.htm   (544 words)

  
 Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts
Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: characterization, direct quantification, and in situ location of transconjugant cells.
The transfer of the plasmids pJKJ5 and TOL (pWWO) from Pseudomonas putida to the indigenous bacterial community on alfalfa sprouts was studied.
Transconjugants carrying either of the investigated plasmids mainly belonged to the genera Pseudomonas and ERWINIA: The numbers of transconjugant cells did not reach detectable levels until 6 days after the onset of germination, at which point these species constituted the majority of the indigenous bacteria.
www.sproutnet.com /Research/plasmid_transfer_from_pseudomona.htm   (331 words)

  
 Pseudomonas putida   (Site not responding. Last check: 2007-11-06)
"Pseudomonad" is a general term for a diverse group of bacteria which morphologically and physiologically resemble members of the genus Pseudomonas.
Most pseudomonads are motile by means of a single polar flagellum, although the agar-grown culture shown this video does not show motility:
putida is non-pathogenic, other pseudomonads can cause disease.
www-micro.msb.le.ac.uk /video/Pputida.html   (296 words)

  
 Metabolic comparison of the in-silico phenotype-genotype relationship of Pseudomonas putida and Pseudomonas aeruginosa   (Site not responding. Last check: 2007-11-06)
The relationship between the genotype and the phenotype is complex, highly non-linear and cannot be predicted from simply cataloguing and assigning gene functions to genes found in a genome.
We report on an in silico representation of Pseudomonas putida and Pseudomonas aeruginosa that describes their metabolic capacities within the scope of their environmental constraints.
Such in silico models can be as well used to choose the most informative knockouts and ultimately, to rationally design experiments relevant for the elucidation of the behavior of these bacteria in polluted environments or within the scope of their relationships (as pathogens) with an infected host.
www.iscb.org /ismb2003/posters/vdsATgbf.de_203.html   (402 words)

  
 EPA: TSCA Environmental Release Application (TERA) for Pseudomonas putida - Biotechnology Program Under Toxic ...
putida is a common, wide-spread, soil bacterium that is not pathogenic to plants or animals.
Since the genetic modifications involved in creating the TERA strain is expected to have no effect on pathogenicity (i.e., on the ability of the microorganisms to infect and to cause disease), it is believed that the strain presents a low ecological hazard for small-scale field testing.
In the TERA approval letter, EPA advised the submitter that this antibiotic is among the least desirable antibiotic resistance markers.
www.epa.gov /docs/opptintr/biotech/pubs/submissions/r010002.htm   (736 words)

  
 Physiological Stress in Batch Cultures of Pseudomonas putida 54G During Toluene Degradation
97-010 Physiological stress associated with toluene exposure in batch cultures of Pseudomonas putida 65G was investigated.
putida 54G cells were grown using a continuous vapor phase feed stream containing 150 ppmv or 750 ppmv toluene as the sole carbon and energy source.
Mirpuri, R.G., W.L. Jones, and H.F. Ridgway, "Physiological Stress in Batch Cultures of Pseudomonas putida 54G During Toluene Degradation," J.
www.erc.montana.edu /res-lib99-sw/pubs/Abstracts/1997/97010.htm   (268 words)

  
 Role of Pseudomonas putida Indoleacetic Acid in Development of the Host Plant Root System -- Patten and Glick 68 (8): ...
putida GR12-2 or with a 1:1 mixture of the wild-type
A mutation in the indole-3-acetic acid biosynthesis pathway of Pseudomonas syringae pv.
Isolation and characterization of mutants of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 that overproduce indoleacetic acid.
aem.asm.org /cgi/content/full/68/8/3795   (4851 words)

  
 Molecular Characterization of the Gallate Dioxygenase from Pseudomonas putida KT2440: THE PROTOTYPE OF A NEW SUBGROUP ...
Molecular Characterization of the Gallate Dioxygenase from Pseudomonas putida KT2440: THE PROTOTYPE OF A NEW SUBGROUP OF EXTRADIOL DIOXYGENASES -- Nogales et al.
putida strain KT2440 (12, 13) was used throughout this study.
putida strains (33, 35), the gene and the enzyme
www.jbc.org /cgi/content/full/280/42/35382   (5018 words)

  
 Roles of dimerization in folding and stability of ketosteroid isomerase from Pseudomonas putida biotype B -- Kim et al. ...
Roles of dimerization in folding and stability of ketosteroid isomerase from Pseudomonas putida biotype B -- Kim et al.
Roles of dimerization in folding and stability of ketosteroid isomerase from Pseudomonas putida biotype B
Bowie, JU and Sauer, R.T. Equilibrium dissociation and unfolding of the arc repressor dimer.
www.proteinscience.org /cgi/content/full/10/4/741   (6416 words)

  
 Epidemiologic Notes and Reports Reported Contamination of Heparin Sodium with Pseudomonas putida   (Site not responding. Last check: 2007-11-06)
On three occasions, from December 1985 to January 1986, Pseudomonas putida was isolated from routine surveillance cultures of bone marrow harvested from three donors at a single hospital in Minnesota.
Heparin sodium without preservatives may be selected for use in clinical situations in which preservatives might have undesirable effects, such as for maintaining patency of intravenous catheters in neonates or anticoagulation of bone marrow harvested for transplantation.
Hospitals that have identified patients with P. putida bloodstream infections in the past year are requested to report their findings through local and state health departments to CDC's Hospital Infections Program, telephone (404) 329-3406.
www.cdc.gov /mmwr/preview/mmwrhtml/00000690.htm   (584 words)

  
 Antibiotic-Dependent Induction of Pseudomonas putida DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding ...
putida DOT-T1E cultures were grown for 4 h in the absence and presence of different antibiotics at sub-MICs: streptomycin (Sm), 8 µg/ml; chloramphenicol (Cm), 30 µg/ml; nalidixic acid (Nal), 30 µg/ml; tetracycline (Tc), 1 µg/ml; carbenicillin (Cb), 120 µg/ml; and gentamicin (Gm), 0.25 µg/ml.
TtgR binds to the ttgABC-ttgR intergenic region and is released in the presence of chloramphenicol and tetracycline.
Cloning, sequencing, and phenotypic characterization of the rpoS gene from Pseudomonas putida KT2440.
aac.asm.org /cgi/content/full/47/10/3067   (4787 words)

  
 Species-specific repetitive extragenic palindromic (REP) sequences in Pseudomonas putida -- Aranda-Olmedo et al. 30 ...
Pseudomonas putida KT2440 carrying the indicated plasmids was grown until exponential phase was reached, and ß-galactosidase was determined as described in Materials and Methods.
Pseudomonas putida REP sequences are favoured in extragenic spaces between convergent genes
Esteve-Núñez,A., Lucchesi,G., Philipp,B., Schink,B. and Ramos,J.L. (2000) Respiration of 2,4,6-trinitrotoluene by Pseudomonas sp.
nar.oxfordjournals.org /cgi/content/full/30/8/1826   (5293 words)

  
 Transcriptional regulation of styrene degradation in Pseudomonas putida CA-3 -- O’Leary et al. 147 (4): 973 -- ...
Hester, K. L., Madhusudhan, K. and Sokatch, J. Catabolite repression control by crc in 2xYT medium is mediated by posttranscriptional regulation of bkdR expression in Pseudomonas putida.
Martinez-Blanco, H., Reglero, A., Rodriquez-Aparacio, L. and Luengo, J. Purification and biochemical characterisation of phenylacetyl-coA ligase from Pseudomonas putida.
Role of the crc Gene in Catabolic Repression of the Pseudomonas putida GPo1 Alkane Degradation Pathway
mic.sgmjournals.org /cgi/content/full/147/4/973   (3866 words)

  
 Metallo-{beta}-Lactamases in Clinical Pseudomonas Isolates in Taiwan and Identification of VIM-3, a Novel Variant of ...
Metallo-{beta}-Lactamases in Clinical Pseudomonas Isolates in Taiwan and Identification of VIM-3, a Novel Variant of the VIM-2 Enzyme -- Yan et al.
putida isolates appeared to carry the MBL gene on plasmids.
Yomoda, S., Okubo, T., Takahashi, A., Murakami, M., Iyobe, S. Presence of Pseudomonas putida Strains Harboring Plasmids Bearing the Metallo-{beta}-Lactamase Gene blaIMP in a Hospital in Japan.
aac.asm.org /cgi/content/abstract/45/8/2224   (1261 words)

  
 Transient XylR binding to the UAS of the Pseudomonas putida {sigma}54 promoter Pu revealed with high intensity UV ...
Abril,M.A., Buck,M. and Ramos,J.L. (1991) Activation of the Pseudomonas TOL plasmid upper pathway operon.
Garmendia,J. and de Lorenzo,V. (2000) The role of the interdomain B linker in the activation of the XylR protein of Pseudomonas putida.
Cases,I. and de Lorenzo,V. (2003) The sigma 54 regulon (sigmulon) of Pseudomonas putida.
nar.oxfordjournals.org /cgi/content/full/31/23/6926   (6390 words)

  
 Molecular Analysis of Pseudomonas aeruginosa Protease IV Expressed in Pseudomonas putida -- Traidej et al. 44 (1): 190 ...
Molecular Analysis of Pseudomonas aeruginosa Protease IV Expressed in Pseudomonas putida -- Traidej et al.
Articles by Traidej, M. Articles by O’Callaghan, R. Molecular Analysis of Pseudomonas aeruginosa Protease IV Expressed in Pseudomonas putida
putida, to elucidate the molecular properties and virulence
www.iovs.org /cgi/content/abstract/44/1/190   (431 words)

  
 Pseudomonas Genome Project: Related Links   (Site not responding. Last check: 2007-11-06)
Pseudomonas 2005: 10th International Congress on Pseudomonas - Palais de Congres, Marseille, France.
Pseudomonas syringae DC3000 annotation project - Provides utility to browse the Pseudomonas syringae DC3000 genomic sequence and current annotation.
Pseudomonas aeruginosa Genome Database (currently inactive) – John Mattick, Institute for Molecular and Cellular Biology, University of Queensland, Brisbane, Australia
www.pseudomonas.com /related_links.html   (393 words)

  
 Isonitrile Hydratase from Pseudomonas putida N19-2. CLONING, SEQUENCING, GENE EXPRESSION, AND IDENTIFICATION OF ITS ...
Isonitrile hydratase is a novel enzyme in Pseudomonas putida N19-2 that catalyzes the conversion of isonitriles to N-substituted
putida N19-2 was isolated previously from soil (20).
putida N19-2 was cultured at 28 °C for 12 h in 20 ml of LB medium
www.jbc.org /cgi/content/full/277/48/45860   (4272 words)

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