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 | | In 20 mM HEPES, pH 7.4, and varying concentrations of chloride and bicarbonate with total anion concentration of 150 mM, SPQ is quenched dynamically by chloride with an apparent Stern-Volmer quenching constant 0.071 (0.016 mM-1 at 25 °C. HEPES alone quenched SPQ fluorescence with a quenching constant of 0.030 (0.003 mM-1. |
 | | The apparent quenching constant for chloride in the presence of 20 mM HEPES and bicarbonate, calculated from equation seq eqn eqnKq 3, is 0.072 mM-1. |
 | | Dynamic quenching of SPQ by chloride and bicarbonate solutions. The fluorescence of a solution of 500 (M SPQ, 20 mM HEPES, pH 7.4, and varying chloride and bicarbonate concentrations (total anion concentration = 150 mM) was measured at 25 °C. The data represent the average of three experiments. |
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