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Topic: RNase H

Related Topics

Reverse transcriptase

Nuclease

PolIII holoenzyme

Phosphodiester bond

DNA replication

Black Rod

Amazon parrot

DNA holoenzyme

	Pfam 18.0 : RnaseH (Site not responding. Last check: 2007-10-09)
		The RNase H domain is responsible for hydrolysis of the RNA portion of RNA x DNA hybrids, and this activity requires the presence of divalent cations (Mg2+ or Mn2+) that bind its active site.
		This domain is a part of a large family of homologous RNase H enzymes of which the RNase HI protein from Escherichia coli is the best characterized MEDLINE:9741851.
		Retroviral RNase H is synthesised as part of the POL polyprotein that contains; an aspartyl protease, a reverse transcriptase, RNase H and integrase.
	pfam.wustl.edu /cgi-bin/getdesc?name=RnaseH (299 words)

	Comparison of Second-Strand Transfer Requirements and RNase H Cleavages Catalyzed by Human Immunodeficiency Virus Type ...
		RNase H cleavages require a second binding event by RT (23).
		The position of RNase H cleavage is indicated by a nick in the RNA strand.
		Catalytically distinct conformations of the ribonuclease H of HIV-1 reverse transcriptase by substrate cleavage patterns and inhibition by azidothymidylate and N-ethylmaleimide.
	jvi.asm.org /cgi/content/full/74/20/9668 (6117 words)

	Isolation of RNase H Genes That Are Essential for Growth of Bacillus subtilis 168 -- Itaya et al. 181 (7): 2118 -- The ... (Site not responding. Last check: 2007-10-09)
		RNase H (EC 3.1.26.4) endonucleolytically cleaves RNA in RNA-DNA hybrid molecules (26).
		to that observed for RNase H of HIV reverse transcriptase (28).
		RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro.
	jb.asm.org /cgi/content/full/181/7/2118 (3513 words)

	Human immunodeficiency virus reverse transcriptase-associated RNase H activity. (Site not responding. Last check: 2007-10-09)
		Biochemical characteristics of the RNase H activity associated with immunoaffinity purified human immunodeficiency virus (HIV) reverse transcriptase (RT) were examined.
		The optimum pH for HIV RNase H activity was between 8.0 and 8.5, in contrast to an optimum pH of 7.5 to 8.0 for HIV RT activity.
		The association of RNase H activity with the p66 component of HIV RT was demonstrated by activity gel analysis.
	www.aegis.com /aidsline/1989/aug/M8980035.html (464 words)

	RNase H Requirements for the Second Strand Transfer Reaction of Human Immunodeficiency Virus Type 1 Reverse ... (Site not responding. Last check: 2007-10-09)
		The catalytic triad of the RNase H domain consists of Asp 443, Glu 478, and Asp 498 (10, 11, 37).
		was the degradation of the 58-mer by RNase H, yielding 5'-end-labeled
		E478Q RT is an endoribonuclease in the presence of manganese and lacks all RNase H activity in the presence of magnesium.
	jvi.asm.org /cgi/content/full/73/8/6573 (6261 words)

	Activation/Attenuation Model for RNase H. A ONE-METAL MECHANISM WITH SECOND-METAL INHIBITION -- Keck et al. 273 (51): ...
		H mechanism is similar to that proposed for DNase I (17), with
		Soluble RNase H Activity Assays-- Synthesis and purification of radiolabeled RNA·DNA hybrid substrate was performed as described previously (27).
		The first model for RNase H activation is a one-metal mechanism analogous to the proposed mechanism for DNase I activity (17).
	www.jbc.org /cgi/content/full/273/51/34128 (4681 words)

	RNase H, RNases and Inhibitors, New England Biolabs
		RNase H, RNases and Inhibitors, New England Biolabs
		RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA.
		Incubation of a 50 μl reaction containing 50 units of RNase H with 1 μg of ΦX174 RF I DNA for 4 hours at 37ºC resulted in
	www.neb.com /nebecomm/products/productM0297.asp (285 words)

	Saccharomyces cerevisiae RNase H(35) Functions in RNA Primer Removal during Lagging-Strand DNA Synthesis, Most ... (Site not responding. Last check: 2007-10-09)
		RNase H(35) protein was purified on a Ni column with an FPLC system, and fractions were analyzed by SDS-polyacrylamide gel electrophoresis.
		In the absence of RNase H(35), Rad27p as a FEN removes the displaced single-stranded RNA primer adjacent to the newly synthesized DNA.
		Structure-specific cleavage of the RNA primer from Okazaki fragments by calf thymus RNase H. Biol.
	mcb.asm.org /cgi/content/full/19/12/8361 (7776 words)

	Novel HIV-1 RNAse H inhibitors (Site not responding. Last check: 2007-10-09)
		This invention relates to novel inhibitors of HIV-1 RT RNAse H. This class of compounds is characterized as small oligonucleotide hairpins.
		HIV-1 RNAse H is critical to the virus life cycle and its replication, rendering it an attractive target for therapeutic intervention.
		The compounds of this invention form the basis of drug discovery and optimization targeted to HIV RNAse H as a viable alternate strategy for therapeutic intervention.
	www.mcgill.ca /ott/technologies/lifesci/03050 (172 words)

	Inhibition of HIV-1 Ribonuclease H by a Novel Diketo Acid, 4-[5-(Benzoylamino)thien-2-yl]-2,4-dioxobutanoic Acid -- ...
		Inhibition of Polymerase-independent RNase H Cleavage-- There are striking structural and functional homologies between HIV-1 integrase and the RNase H domain of HIV-1 RT.
		In RNase H cleavage assays, the activity of Compound I against full-length wild type HIV-1 RT (IC, Table I) was
		RNase H domain and demonstrate a requirement for divalent cation.
	www.jbc.org /cgi/content/full/278/5/2777 (3408 words)

	Cambio Ltd : Mutation Discovery and Screening : Hybridase Thermostable RNase H (Site not responding. Last check: 2007-10-09)
		In contrast to E. coli RNaseH, which is rapidly inactivated at 55°C, Hybridase RNase H is active at high temperatures.
		Unit Definition: One unit of Hybridase RNase H results in the acid-solubilisation of 1nmole of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50mM Tris-HCl (pH 7.5), 100mM NaCl, and 10mM MgCl
		Quality Control: Hybridase Thermostable RNase H is tested for RNA degradation in an RNA:DNA hybrid and for the absence of detectable DNA exo- or endonuclease, and non-RNase H RNase activities.
	www.cambio.co.uk /l3.php?l1_category_id=7&l2_category_id=140 (343 words)

	Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H -- Wisniewski et al. 97 (22): 11978 -- ...
		RNase H is the complete removal of the original genomic RNA during
		An RNase H time course assay of the primary and secondary cuts using an excess of substrate to enzyme.
		The polymerase-independent mode of RNase H activity is also a key component of the minus strand transfer reaction.
	www.pnas.org /cgi/content/full/97/22/11978 (4408 words)

	Metal binding and activation of the ribonuclease H domain from Moloney murine leukemia virus -- Goedken and Marqusee 12 ...
		H domain from HIV RT, however, is inactive.
		RNase H activity is decreased in all mutants
		H, and because Mn is the optimal metal for this RT, we investigated
	peds.oxfordjournals.org /cgi/content/full/12/11/975 (3579 words)

	Reverse transcriptase - EvoWiki (Site not responding. Last check: 2007-10-09)
		Reverse transcriptase produces a complementary DNA strand to produce a double helix DNA/RNA hybrid.
		RNase H then degrades the RNA chain and DNA directed DNA polymerase replaces the RNA chain with a complementary DNA chain.
		The reverse transcriptase coded by HIV has an error rate as high as 1 in 2000 bases, accounting for the rapid evolution of HIV, and difficulty in developing effective drugs.
	www.evowiki.org /index.php/Reverse_transcriptase (207 words)

	[No title] (Site not responding. Last check: 2007-10-09)
		Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA of DNA-RNA hybrids, producing 5’ phosphate-terminated oligoribonucleotides that are 2-9 bases in length.
		Stratagene purifies RNase H to homogeneity from bacteria expressing the E.
		Stratagene’s RNase H is available in two convenient unit concentrations: 2 U/µl and 10 U/ml.
	www.stratagene.com /products/displayProduct.aspx?pid=16 (151 words)

	Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA -- Sarafianos et al. 20 ... (Site not responding. Last check: 2007-10-09)
		Champoux,J.J., Gilboa,E. and Baltimore,D. (1984) Mechanism of RNA primer removal by the RNase H activity of avian myeloblastosis virus reverse transcriptase.
		Resnick,R., Omer,C.A. and Faras,A.J. (1984) Involvement of retrovirus RT-associated RNase H in the initiation of strong-stop (+) DNA synthesis and the generation of the long terminal repeat.
		Yang,W., Hendrickson,W.A., Crouch,R.J. and Satow,Y. (1990) Structure of RNase H phased at 2 Å resolution by MAD analysis of the selenomethionyl protein.
	embojournal.npgjournals.com /cgi/content/full/20/6/1449 (7355 words)

	Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7 -- Ohtani et al. ...
		Drolet,M., Phoenix,P., Menzel,R., Masse,E., Liu,L.F. and Crouch,R.J. ((1995)) Overexpression of RNase H partially complements the growth defect of an Escherichia coli delta topA mutant: R-loop formation is a major problem in the absence of DNA topoisomerase I. Proc.
		Arudchandran,A., Cerritelli,S., Narimatsu,S., Itaya,M., Shin,D.Y., Shimada,Y. and Crouch,R.J. ((2000)) The absence of ribonuclease H1 or H2 alters the sensitivity of Saccharomyces cerevisiae to hydroxyurea, caffeine and ethyl methanesulphonate: implications for roles of RNases H in DNA replication and repair.
		Itaya,M., Omori,A., Kanaya,S., Crouch,R.J., Tanaka,T. and Kondo,K. ((1999)) Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
	nar.oxfordjournals.org /cgi/content/full/32/19/5809 (5733 words)

	Replication Errors during In Vivo Ty1 Transposition Are Linked to Heterogeneous RNase H Cleavage Sites -- Mules et al. ...
		RNase H cleavage of the 5' end of the RNA exposes minus-strand cDNA sequences complementary to the repeat (R) region and plays
		Cleavage specificities of Moloney murine leukemia virus RNase H implicated in the second strand transfer during reverse transcription.
		Abortive reverse transcription by mutants of Moloney murine leukemia virus deficient in the reverse transcriptase-associated RNase H function.
	mcb.asm.org /cgi/content/full/18/2/1094 (6646 words)

	ipedia.com: Reverse transcriptase Article (Site not responding. Last check: 2007-10-09)
		An example of this kind of enzyme is the reverse transcriptase from the human immunodeficiency virus type 1 (PDB 1HMV, EC 2.7.7.49).
		In addition to the transcription function, retroviral reverse transcriptase carries a RNase domain, which belongs to the RNase H family.
		Reverse transcriptase is commonly used in the field of research to be able to apply the polymerase chain reaction technique to RNA.
	www.ipedia.com /reverse_transcriptase.html (221 words)

	Molecular Cytogenetics and Genome Organization - Pat Heslop-Harrison
		Carvalho A, Guedes-Pinto H, Martin A, Heslop-Harrison P, Lima-Brito J. Genome discrimination and chromosome pairing in the Hordeum chilense × Aegilops tauschii amphiploid.
		The H. chilense total genomic DNA and the ribosomal sequence pTa71 probes, allowed the unequivocal discrimination of the 14 chromosomes of Hch genome-origin and the 14 chromosomes of D genome-origin.
		Chaves R, Heslop-Harrison JS, Guedes-Pinto H. Centromeric heterochromatin in the cattle rob(1;29) translocation: alpha satellite I sequences, in situ Msp I digestion patterns, chromomycin staining and C-bands.
	www.le.ac.uk /biology/phh4/titleabst.htm (13464 words)

	BioAfrica - HIV-1 Reverse Transcriptase [(p66) RT and RNase H] sequence, structure and function information
		BioAfrica - HIV-1 Reverse Transcriptase [(p66) RT and RNase H] sequence, structure and function information
		HIV RT/RNase H forms a heterodimer with RT/RNase H polyprotein and reverse transcribes viral RNA into DNA.
		RNase H activity removes RNA strand from RNA/DNA duplex
	www.bioafrica.net /proteomics/POL-p66prot.html (518 words)

	BioAfrica - HIV-1 RNase H (RNH) sequence, structure and function information
		BioAfrica - HIV-1 RNase H (RNH) sequence, structure and function information
		PDB: 1o1w (HIV-1 RNase H) SwissProt: P04585 (HIV-1 HXB2 POL)
		HIV RNase H removes the original RNA template from the first DNA strand allowing synthesis of the complementary strand of DNA.
	www.bioafrica.net /proteomics/POL-RNHprot.html (307 words)

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