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Topic: RRNA


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In the News (Fri 13 Nov 09)

  
  Ribosomal RNA - Wikipedia, the free encyclopedia
Ribosomal RNA (rRNA) is a type of RNA that is the central component of the ribosome, the protein manufacturing machinery of all living cells.
The function of the rRNA is to provide a mechanism for decoding mRNA into amino acids (at center of small ribosomal subunit) and to interact with the tRNAs during translation by providing peptidyl transferase activity (large subunit).
The rRNA genes of the other parent are suppressed and not generally transcribed, though reactivation of the suppressed or 'inferior' rRNA genes may occasionally occur.
en.wikipedia.org /wiki/RRNA   (423 words)

  
 Non-coding RNA - Wikipedia, the free encyclopedia
The most prominent examples of non-coding RNAs are transfer RNA (tRNA) and ribosomal RNA (rRNA), both of which are involved in the process of translation and gene expression.
Ribosomal RNA (rRNA) is the primary constituent of ribosomes.
Cytoplasmic rRNA genes are highly repetitive because of huge demand of ribosomes for protein synthesis ('gene dosage') in the cell.
en.wikipedia.org /wiki/Non-coding_RNA   (1248 words)

  
 rRNA   (Site not responding. Last check: 2007-10-08)
Ribosomal RNA (rRNA) is a component of the ribosomes, the protein synthetic factories in the cell.
Three of the rRNA molecules are synthesized in the nucleolus, and one is synthesized elsewhere.
These rRNA molecules and the 5 s rRNA combine with the ribosomal proteins in the nucleolus to form pre 40 s and pre 60 s ribosomal subunits.
www.biochem.uwo.ca /meds/medna/rRNA.html   (240 words)

  
 rRNA or proteins for phylogeny?
In both 16S and 23S rRNA unrooted trees, the length of the archaeal branch (i.e., the branch connecting the last common ancestor of all archaea to the point of trifurcation) is strikingly shorter than the bacterial and eucaryal branches (see Fig.
For example, while 18S rRNA analyses have suggested that Microsporidia are one of the most ancient eukaryotic lineages, recent analyses of their proteins performed by Keeling and Doolittle in Halifax (Canada) and by Hasegawa in Tokyo (Institute of Statistical Mathematics) indicate that they are close relatives of fungi.
Studies based on protein sequences of heatshock (HSP70) proteins and chaperonins have provided evidence that, firstly, the amitochondriate organisms all have had a mitochondrion which may have been lost secondarily, or that was converted from a mitochondrion to an anaerobic hydrogenosome as is the case in the trichomonads, belonging to the Parabasalia.
www.icp.ucl.ac.be /~opperd/private/tree_o_l.html   (792 words)

  
 Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach ...   (Site not responding. Last check: 2007-10-08)
on small-subunit (SSU) rRNA and the rRNA gene (6).
Percentages of cleaved 16S rRNA in the total 16S rRNA were directly estimated based on the peak areas of intact and cleaved 16S rRNA fragments in the electro- pherograms, and the percentages were plotted with the hybridization and digestion temperatures at which the respective reactions were performed.
Percentages of cleaved 16S rRNA in the total 16S rRNA were calculated in the same manner used for the graph in panel B and were plotted along with the hybridization and digestion temperatures used.
aem.asm.org /cgi/content/full/70/6/3650   (8223 words)

  
 RNase L-Independent Specific 28S rRNA Cleavage in Murine Coronavirus-Infected Cells -- Banerjee et al. 74 (19): 8793 -- ...
Coronaviruses are enveloped RNA viruses that cause gastrointestinal and upper respiratory tract illnesses in animals and humans.
This rRNA degradation is mediated by the 2-5A
Cytoplasmic RNA was extracted at 8 h p.i., and 28S rRNA cleavage was examined by Northern blot analysis using 5'-end-labeled probe 1.
jvi.asm.org /cgi/content/full/74/19/8793   (6717 words)

  
 lsu rRNA database   (Site not responding. Last check: 2007-10-08)
A database on the structure of lsu ribosomal subunit RNA (further abbreviated as lsu rRNA) is being maintained in our research group and made available to the scientific community.
The database includes indications of secondary structure elements for each sequence, based on the adopted rRNA secondary structure, which in turn is corroborated by the observation of compensating substitutions in the alignment.
Any selection of lsu ribosomal subunit rRNA sequences in our database can be easily obtained in a number of formats via the forms or the query interface.
www.psb.ugent.be /rRNA/lsu   (261 words)

  
 rRNA complementarity within mRNAs: A possible basis for mRNA-ribosome interactions and translational control -- Tranque ...
The positions of the rRNAs were determined by ethidium bromide staining of the agarose gels.
After cross-linking the probes to ribosomes, as described in Materials and Methods, the RNAs were purified away from protein, annealed to one of the oligonucleotides, and the rRNA at this position was digested with RNase H. The positions of the rRNAs were determined by ethidium bromide staining of the agarose gels.
The secondary structure of the 18S rRNA at nucleotides 1835-1865 is represented as determined in situ by Holmberg et al.
www.pnas.org /cgi/content/full/95/21/12238   (4640 words)

  
 Rearranged Subtelomeric rRNA Genes in Giardia duodenalis -- Upcroft et al. 4 (2): 484 -- Eukaryotic Cell   (Site not responding. Last check: 2007-10-08)
The rRNA gene unit is inserted into the spacer separating it from the TGU at position 2523 in the LS rRNA (5, 10, 15).
The rRNA gene unit repeat notation defines the last base of the unit (5566) within the SS rRNA gene.
Chromosomes of Giardia strain WB were separated in the first dimension (D1) as indicated by a vertical arrow (A) and after NotI cleavage in the second dimension (D2) as indicated by a horizontal arrow (15).
ec.asm.org /cgi/content/full/4/2/484   (1917 words)

  
 An Escherichia coli strain with all chromosomal rRNA operons inactivated: Complete exchange of rRNA genes between ...
The amount of total RNA was obtained by measuring the absorption at 260 nm of RNA hydrolysates as described in refs.
Inactivated rRNA operons are indicated by capital letters derived from their specific operon names (for example, A for rrnA).
DNA primers annealed to 16S rRNA molecules were extended in the presence of one dideoxynucleotide (ddATP) and three deoxynucleotides (dGTP, dCTP, and dTTP).
www.pnas.org /cgi/content/full/96/5/1971   (4694 words)

  
 Life History Implications of rRNA Gene Copy Number in Escherichia coli -- Stevenson and Schmidt 70 (11): 6670 -- ...
Outline of procedure used to delete the rRNA operon rrnA from the chromosome of E.
Homologous recombination events are depicted as crossed dotted lines, which encompass regions of homology; plasmid-derived regions of DNA are depicted in grey.
Locations of hybridized bands corresponding to the 16S rRNA gene from each rRNA operon (bands A through E, G, and H) are indicated for the parental strain.
aem.asm.org /cgi/content/full/70/11/6670   (5421 words)

  
 rRNA definition - Medical Dictionary definitions of popular medical terms
rRNA: Ribosomal RNA, a molecular component of a ribosome, the cell's essential protein factory.
A tiny particulate structure located in the cytoplasm of the cell (outside the nucleus), the ribosome is composed of two subunits, one larger than the other.
Although the ribosome consists of both ribosomal RNA (rRNA) and protein, the "active site" on the large unit of the ribosome -- the site of the chemical reaction that changes genetic information into the beginnings of a protein -- contains only rRNA.
www.medicinenet.com /script/main/art.asp?articlekey=16316   (246 words)

  
 Changes in rRNA Levels during Stress Invalidates Results from mRNA Blotting: Fluorescence In Situ rRNA Hybridization ...
The oligonucleotide probes used in the present study are shown in Table 1.
The values were calculated by multiplying the cellular levels of rRNA shown in Fig.
RNA slot blotting was used to estimate the amounts of 16S and 23S rRNA during heat shock by using a standard procedure.
jb.asm.org /cgi/content/full/183/16/4747   (3932 words)

  
 Atypical Processing in Domain III of 23S rRNA of Rhizobium leguminosarum ATCC 10004T at a Position Homologous to an ...
A part of the rRNA sequence is indicated on the side of the panel, and the position of the mature 3' end of the proximal 1.3-kb rRNA fragment is shown by an arrow.
is absent from the proximal 1.3-kb rRNA fragment.
Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp.
jb.asm.org /cgi/content/full/184/12/3176   (6219 words)

  
 RecA-Mediated Gene Conversion and Aminoglycoside Resistance in Strains Heterozygous for rRNA -- Prammananan et al. 43 ...
Analysis of 16S rRNA position 1408 was performed by amplification of genomic DNA with primer 285 and primer 261 (the primers
Analysis of the 23S rRNA genes of transformants grown on LB medium and LB medium containing clarithromycin was performed and
rRNA operons and a heterozygous rRNA genotype, RecA-mediated gene
aac.asm.org /cgi/content/full/43/3/447   (4387 words)

  
 Use of 16S rRNA, 23S rRNA, and gyrB Gene Sequence Analysis To Determine Phylogenetic Relationships of Bacillus cereus ...
We did not include in Table 3 the substitutions of A for G, T, and C reported earlier for the 23S rRNA sequences of B.
Superscript letters: a, 16S rRNA sequences are available (Table 2); b, whole-genome sequences are available (Table 3); c, 5 of 11 23S rRNA genes contain T(594); d, 4 of 12 23S RNA genes contain the insertion G(1218-129); e, 6 of 12 23S rRNA genes have A(1559); f, 7 of 11 23S rRNA genes in B.
Names of the subgroups were identified through 16S rRNA sequences of their members (Table 4) and are displayed with bold on the right sides of the brackets.
jcm.asm.org /cgi/content/full/42/8/3711   (6836 words)

  
 SSU rRNA Secondary Structure Prediction and Alignment Main   (Site not responding. Last check: 2007-10-08)
This server allows you to enter an RNA sequence and predict the secondary structure interactions interpreting the sequence as a bacterial small subunit ribosomal RNA.
If a non-bacterial SSU rRNA sequence is submitted then the grammar will try to interpret the sequence as bacterial as best it can.
If a segment of SSU rRNA sequence is submitted then the grammar will try to interpret the segment as the full sequence and will "spread" the segment across the grammar.
www.cse.ucsc.edu /research/compbio/ssurrna.html   (423 words)

  
 Ribosomal Database Project II - Release 9
Use the RDP Pipeline to process sequence libraries from raw sequencer output to analysis.
Hierarchy Browser - Browse a phylogenetic hierarchy and compile a list of 16S rRNA sequences for download or use.
Classifier - Assign 16S rRNA sequences to our taxonomical hierarchy.
rdp.cme.msu.edu   (240 words)

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